This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.10 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 338,886 (13.9%) Reads that were too short: 11,733 (0.5%) Reads written (passing filters): 327,153 (13.4%) Total basepairs processed: 735,190,694 bp Total written (filtered): 93,501,163 bp (12.7%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 338886 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1786 596.3 0 1786 7 2287 596.3 0 2287 8 140859 596.3 0 140859 9 164449 596.3 0 164449 10 157 596.3 0 157 11 48 596.3 0 48 12 34 596.3 0 34 13 32 596.3 0 32 14 22 596.3 0 22 15 25 596.3 0 25 16 29 596.3 0 29 17 21 596.3 0 21 18 23 596.3 0 23 19 21 596.3 0 21 20 24 596.3 0 24 21 24 596.3 0 24 22 26 596.3 0 26 23 30 596.3 0 30 24 37 596.3 0 37 25 35 596.3 0 35 26 27 596.3 0 27 27 29 596.3 0 29 28 28 596.3 0 28 29 33 596.3 0 33 30 34 596.3 0 34 31 22 596.3 0 22 32 18 596.3 0 18 33 14 596.3 0 14 34 30 596.3 0 30 35 25 596.3 0 25 36 42 596.3 0 42 37 23 596.3 0 23 38 33 596.3 0 33 39 27 596.3 0 27 40 37 596.3 0 37 41 24 596.3 0 24 42 38 596.3 0 38 43 27 596.3 0 27 44 25 596.3 0 25 45 29 596.3 0 29 46 24 596.3 0 24 47 23 596.3 0 23 48 26 596.3 0 26 49 36 596.3 0 36 50 21 596.3 0 21 51 28 596.3 0 28 52 33 596.3 0 33 53 26 596.3 0 26 54 22 596.3 0 22 55 26 596.3 0 26 56 26 596.3 0 26 57 33 596.3 0 33 58 26 596.3 0 26 59 22 596.3 0 22 60 19 596.3 0 19 61 23 596.3 0 23 62 29 596.3 0 29 63 33 596.3 0 33 64 28 596.3 0 28 65 34 596.3 0 34 66 32 596.3 0 32 67 44 596.3 0 44 68 30 596.3 0 30 69 114 596.3 0 114 70 43 596.3 0 43 71 177 596.3 0 177 72 30 596.3 0 30 73 25 596.3 0 25 74 35 596.3 0 35 75 29 596.3 0 29 76 55 596.3 0 55 77 61 596.3 0 61 78 83 596.3 0 83 79 55 596.3 0 55 80 484 596.3 0 484 81 103 596.3 0 103 82 147 596.3 0 147 83 49 596.3 0 49 84 32 596.3 0 32 85 41 596.3 0 41 86 44 596.3 0 44 87 35 596.3 0 35 88 28 596.3 0 28 89 32 596.3 0 32 90 33 596.3 0 33 91 44 596.3 0 44 92 49 596.3 0 49 93 53 596.3 0 53 94 47 596.3 0 47 95 61 596.3 0 61 96 823 596.3 0 823 97 717 596.3 0 717 98 910 596.3 0 910 99 61 596.3 0 61 100 53 596.3 0 53 101 39 596.3 0 39 102 31 596.3 0 31 103 38 596.3 0 38 104 30 596.3 0 30 105 41 596.3 0 41 106 37 596.3 0 37 107 25 596.3 0 25 108 34 596.3 0 34 109 42 596.3 0 42 110 46 596.3 0 46 111 31 596.3 0 31 112 41 596.3 0 41 113 60 596.3 0 60 114 50 596.3 0 50 115 31 596.3 0 31 116 36 596.3 0 36 117 26 596.3 0 26 118 26 596.3 0 26 119 40 596.3 0 40 120 40 596.3 0 40 121 26 596.3 0 26 122 31 596.3 0 31 123 39 596.3 0 39 124 46 596.3 0 46 125 29 596.3 0 29 126 63 596.3 0 63 127 74 596.3 0 74 128 81 596.3 0 81 129 93 596.3 0 93 130 423 596.3 0 423 131 162 596.3 0 162 132 231 596.3 0 231 133 172 596.3 0 172 134 134 596.3 0 134 135 46 596.3 0 46 136 45 596.3 0 45 137 56 596.3 0 56 138 78 596.3 0 78 139 199 596.3 0 199 140 40 596.3 0 40 141 41 596.3 0 41 142 83 596.3 0 83 143 57 596.3 0 57 144 34 596.3 0 34 145 31 596.3 0 31 146 51 596.3 0 51 147 96 596.3 0 96 148 1246 596.3 0 1246 149 286 596.3 0 286 150 261 596.3 0 261 151 24 596.3 0 24 152 31 596.3 0 31 153 33 596.3 0 33 154 54 596.3 0 54 155 32 596.3 0 32 156 34 596.3 0 34 157 38 596.3 0 38 158 46 596.3 0 46 159 37 596.3 0 37 160 37 596.3 0 37 161 42 596.3 0 42 162 37 596.3 0 37 163 29 596.3 0 29 164 45 596.3 0 45 165 28 596.3 0 28 166 24 596.3 0 24 167 39 596.3 0 39 168 15 596.3 0 15 169 27 596.3 0 27 170 33 596.3 0 33 171 42 596.3 0 42 172 33 596.3 0 33 173 41 596.3 0 41 174 43 596.3 0 43 175 40 596.3 0 40 176 32 596.3 0 32 177 38 596.3 0 38 178 55 596.3 0 55 179 44 596.3 0 44 180 50 596.3 0 50 181 42 596.3 0 42 182 33 596.3 0 33 183 60 596.3 0 60 184 77 596.3 0 77 185 115 596.3 0 115 186 168 596.3 0 168 187 991 596.3 0 991 188 2338 596.3 0 2338 189 743 596.3 0 743 190 358 596.3 0 358 191 53 596.3 0 53 192 42 596.3 0 42 193 26 596.3 0 26 194 33 596.3 0 33 195 28 596.3 0 28 196 28 596.3 0 28 197 36 596.3 0 36 198 46 596.3 0 46 199 32 596.3 0 32 200 34 596.3 0 34 201 38 596.3 0 38 202 33 596.3 0 33 203 40 596.3 0 40 204 30 596.3 0 30 205 33 596.3 0 33 206 42 596.3 0 42 207 52 596.3 0 52 208 40 596.3 0 40 209 252 596.3 0 252 210 299 596.3 0 299 211 395 596.3 0 395 212 86 596.3 0 86 213 27 596.3 0 27 214 47 596.3 0 47 215 32 596.3 0 32 216 40 596.3 0 40 217 50 596.3 0 50 218 39 596.3 0 39 219 58 596.3 0 58 220 44 596.3 0 44 221 35 596.3 0 35 222 33 596.3 0 33 223 74 596.3 0 74 224 59 596.3 0 59 225 78 596.3 0 78 226 148 596.3 0 148 227 106 596.3 0 106 228 77 596.3 0 77 229 54 596.3 0 54 230 103 596.3 0 103 231 55 596.3 0 55 232 61 596.3 0 61 233 104 596.3 0 104 234 102 596.3 0 102 235 160 596.3 0 160 236 120 596.3 0 120 237 88 596.3 0 88 238 74 596.3 0 74 239 132 596.3 0 132 240 201 596.3 0 201 241 197 596.3 0 197 242 380 596.3 0 380 243 360 596.3 0 360 244 137 596.3 0 137 245 61 596.3 0 61 246 321 596.3 0 321 247 120 596.3 0 120 248 108 596.3 0 108 249 261 596.3 0 261 250 241 596.3 0 241 251 367 596.3 0 367 252 139 596.3 0 139 253 334 596.3 0 334 254 758 596.3 0 758 255 631 596.3 0 631 256 153 596.3 0 153 257 72 596.3 0 72 258 85 596.3 0 85 259 86 596.3 0 86 260 127 596.3 0 127 261 123 596.3 0 123 262 268 596.3 0 268 263 63 596.3 0 63 264 66 596.3 0 66 265 84 596.3 0 84 266 77 596.3 0 77 267 46 596.3 0 46 268 140 596.3 0 140 269 71 596.3 0 71 270 144 596.3 0 144 271 168 596.3 0 168 272 177 596.3 0 177 273 171 596.3 0 171 274 176 596.3 0 176 275 96 596.3 0 96 276 30 596.3 0 30 277 28 596.3 0 28 278 38 596.3 0 38 279 40 596.3 0 40 280 43 596.3 0 43 281 58 596.3 0 58 282 61 596.3 0 61 283 96 596.3 0 96 284 47 596.3 0 47 285 53 596.3 0 53 286 28 596.3 0 28 287 35 596.3 0 35 288 38 596.3 0 38 289 35 596.3 0 35 290 49 596.3 0 49 291 30 596.3 0 30 292 54 596.3 0 54 293 50 596.3 0 50 294 61 596.3 0 61 295 53 596.3 0 53 296 55 596.3 0 55 297 63 596.3 0 63 298 69 596.3 0 69 299 42 596.3 0 42 300 41 596.3 0 41 301 225 596.3 0 225 Finished in 14.47 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 327,153 Reads with adapters: 140,039 (42.8%) Reads that were too short: 281 (0.1%) Reads written (passing filters): 139,758 (42.7%) Total basepairs processed: 93,501,163 bp Total written (filtered): 38,570,657 bp (41.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 140039 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 31 0.0 3 0 0 29 2 16 1162 0.0 3 0 1137 22 3 17 137931 0.0 3 134771 3029 117 14 18 294 0.0 3 15 265 13 1 19 88 0.0 3 1 2 75 10 20 209 0.0 3 128 3 0 78 21 1 0.0 3 1 24 1 0.0 3 1 32 1 0.0 3 1 34 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 70 9 0.0 3 0 0 0 9 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 216 7 0.0 3 0 0 0 7 217 216 0.0 3 0 1 1 214 218 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 11 0.0 3 0 0 0 11 281 8 0.0 3 0 0 0 8 282 13 0.0 3 0 0 0 13 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.89 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 139,758 Reads with adapters: 1,133 (0.8%) Reads written (passing filters): 139,758 (100.0%) Total basepairs processed: 38,570,657 bp Total written (filtered): 38,567,039 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1133 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.6% C: 7.6% G: 6.6% T: 4.1% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 998 2183.7 0 998 4 108 545.9 0 108 5 18 136.5 0 18 6 1 34.1 0 1 12 8 0.0 1 0 8 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.