This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.57 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 67,507 (2.8%) Reads that were too short: 12,209 (0.5%) Reads written (passing filters): 55,298 (2.3%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,454,675 bp (2.0%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 67507 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 539 596.3 0 539 7 21251 596.3 0 21251 8 17094 596.3 0 17094 9 42 596.3 0 42 10 69 596.3 0 69 11 24 596.3 0 24 12 24 596.3 0 24 13 18 596.3 0 18 14 27 596.3 0 27 15 18 596.3 0 18 16 15 596.3 0 15 17 17 596.3 0 17 18 13 596.3 0 13 19 25 596.3 0 25 20 33 596.3 0 33 21 33 596.3 0 33 22 25 596.3 0 25 23 20 596.3 0 20 24 25 596.3 0 25 25 17 596.3 0 17 26 11 596.3 0 11 27 12 596.3 0 12 28 11 596.3 0 11 29 18 596.3 0 18 30 27 596.3 0 27 31 17 596.3 0 17 32 23 596.3 0 23 33 14 596.3 0 14 34 16 596.3 0 16 35 22 596.3 0 22 36 36 596.3 0 36 37 35 596.3 0 35 38 71 596.3 0 71 39 37 596.3 0 37 40 66 596.3 0 66 41 21 596.3 0 21 42 25 596.3 0 25 43 35 596.3 0 35 44 19 596.3 0 19 45 18 596.3 0 18 46 15 596.3 0 15 47 15 596.3 0 15 48 157 596.3 0 157 49 48 596.3 0 48 50 21 596.3 0 21 51 28 596.3 0 28 52 13 596.3 0 13 53 33 596.3 0 33 54 22 596.3 0 22 55 35 596.3 0 35 56 24 596.3 0 24 57 21 596.3 0 21 58 16 596.3 0 16 59 25 596.3 0 25 60 52 596.3 0 52 61 59 596.3 0 59 62 36 596.3 0 36 63 19 596.3 0 19 64 40 596.3 0 40 65 33 596.3 0 33 66 26 596.3 0 26 67 27 596.3 0 27 68 45 596.3 0 45 69 175 596.3 0 175 70 252 596.3 0 252 71 171 596.3 0 171 72 426 596.3 0 426 73 615 596.3 0 615 74 308 596.3 0 308 75 31 596.3 0 31 76 19 596.3 0 19 77 18 596.3 0 18 78 27 596.3 0 27 79 28 596.3 0 28 80 48 596.3 0 48 81 87 596.3 0 87 82 146 596.3 0 146 83 128 596.3 0 128 84 368 596.3 0 368 85 1098 596.3 0 1098 86 2418 596.3 0 2418 87 30 596.3 0 30 88 22 596.3 0 22 89 21 596.3 0 21 90 75 596.3 0 75 91 61 596.3 0 61 92 26 596.3 0 26 93 20 596.3 0 20 94 24 596.3 0 24 95 17 596.3 0 17 96 37 596.3 0 37 97 51 596.3 0 51 98 21 596.3 0 21 99 31 596.3 0 31 100 33 596.3 0 33 101 32 596.3 0 32 102 39 596.3 0 39 103 27 596.3 0 27 104 25 596.3 0 25 105 30 596.3 0 30 106 24 596.3 0 24 107 22 596.3 0 22 108 32 596.3 0 32 109 20 596.3 0 20 110 71 596.3 0 71 111 304 596.3 0 304 112 334 596.3 0 334 113 30 596.3 0 30 114 25 596.3 0 25 115 28 596.3 0 28 116 24 596.3 0 24 117 19 596.3 0 19 118 20 596.3 0 20 119 18 596.3 0 18 120 16 596.3 0 16 121 30 596.3 0 30 122 27 596.3 0 27 123 37 596.3 0 37 124 52 596.3 0 52 125 31 596.3 0 31 126 18 596.3 0 18 127 21 596.3 0 21 128 22 596.3 0 22 129 28 596.3 0 28 130 34 596.3 0 34 131 53 596.3 0 53 132 58 596.3 0 58 133 47 596.3 0 47 134 28 596.3 0 28 135 24 596.3 0 24 136 32 596.3 0 32 137 30 596.3 0 30 138 37 596.3 0 37 139 31 596.3 0 31 140 22 596.3 0 22 141 27 596.3 0 27 142 50 596.3 0 50 143 209 596.3 0 209 144 60 596.3 0 60 145 86 596.3 0 86 146 75 596.3 0 75 147 131 596.3 0 131 148 152 596.3 0 152 149 104 596.3 0 104 150 214 596.3 0 214 151 83 596.3 0 83 152 82 596.3 0 82 153 72 596.3 0 72 154 21 596.3 0 21 155 34 596.3 0 34 156 80 596.3 0 80 157 103 596.3 0 103 158 119 596.3 0 119 159 38 596.3 0 38 160 29 596.3 0 29 161 32 596.3 0 32 162 28 596.3 0 28 163 31 596.3 0 31 164 27 596.3 0 27 165 34 596.3 0 34 166 37 596.3 0 37 167 35 596.3 0 35 168 129 596.3 0 129 169 100 596.3 0 100 170 133 596.3 0 133 171 48 596.3 0 48 172 23 596.3 0 23 173 24 596.3 0 24 174 37 596.3 0 37 175 36 596.3 0 36 176 199 596.3 0 199 177 151 596.3 0 151 178 237 596.3 0 237 179 23 596.3 0 23 180 17 596.3 0 17 181 22 596.3 0 22 182 27 596.3 0 27 183 49 596.3 0 49 184 101 596.3 0 101 185 30 596.3 0 30 186 36 596.3 0 36 187 92 596.3 0 92 188 118 596.3 0 118 189 114 596.3 0 114 190 64 596.3 0 64 191 51 596.3 0 51 192 32 596.3 0 32 193 32 596.3 0 32 194 58 596.3 0 58 195 82 596.3 0 82 196 160 596.3 0 160 197 314 596.3 0 314 198 708 596.3 0 708 199 600 596.3 0 600 200 98 596.3 0 98 201 53 596.3 0 53 202 233 596.3 0 233 203 409 596.3 0 409 204 442 596.3 0 442 205 396 596.3 0 396 206 193 596.3 0 193 207 46 596.3 0 46 208 42 596.3 0 42 209 78 596.3 0 78 210 83 596.3 0 83 211 52 596.3 0 52 212 66 596.3 0 66 213 158 596.3 0 158 214 154 596.3 0 154 215 162 596.3 0 162 216 92 596.3 0 92 217 66 596.3 0 66 218 74 596.3 0 74 219 83 596.3 0 83 220 51 596.3 0 51 221 51 596.3 0 51 222 124 596.3 0 124 223 773 596.3 0 773 224 655 596.3 0 655 225 476 596.3 0 476 226 40 596.3 0 40 227 33 596.3 0 33 228 44 596.3 0 44 229 34 596.3 0 34 230 22 596.3 0 22 231 22 596.3 0 22 232 21 596.3 0 21 233 33 596.3 0 33 234 65 596.3 0 65 235 67 596.3 0 67 236 74 596.3 0 74 237 55 596.3 0 55 238 113 596.3 0 113 239 88 596.3 0 88 240 50 596.3 0 50 241 79 596.3 0 79 242 169 596.3 0 169 243 106 596.3 0 106 244 113 596.3 0 113 245 160 596.3 0 160 246 145 596.3 0 145 247 52 596.3 0 52 248 50 596.3 0 50 249 37 596.3 0 37 250 61 596.3 0 61 251 48 596.3 0 48 252 43 596.3 0 43 253 47 596.3 0 47 254 37 596.3 0 37 255 52 596.3 0 52 256 47 596.3 0 47 257 82 596.3 0 82 258 146 596.3 0 146 259 207 596.3 0 207 260 192 596.3 0 192 261 61 596.3 0 61 262 52 596.3 0 52 263 49 596.3 0 49 264 43 596.3 0 43 265 39 596.3 0 39 266 33 596.3 0 33 267 31 596.3 0 31 268 28 596.3 0 28 269 34 596.3 0 34 270 70 596.3 0 70 271 256 596.3 0 256 272 193 596.3 0 193 273 166 596.3 0 166 274 48 596.3 0 48 275 38 596.3 0 38 276 54 596.3 0 54 277 104 596.3 0 104 278 112 596.3 0 112 279 76 596.3 0 76 280 44 596.3 0 44 281 77 596.3 0 77 282 214 596.3 0 214 283 293 596.3 0 293 284 439 596.3 0 439 285 114 596.3 0 114 286 117 596.3 0 117 287 78 596.3 0 78 288 45 596.3 0 45 289 48 596.3 0 48 290 40 596.3 0 40 291 36 596.3 0 36 292 84 596.3 0 84 293 65 596.3 0 65 294 72 596.3 0 72 295 54 596.3 0 54 296 90 596.3 0 90 297 207 596.3 0 207 298 347 596.3 0 347 299 434 596.3 0 434 300 153 596.3 0 153 301 78 596.3 0 78 Finished in 2.90 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 55,298 Reads with adapters: 19,135 (34.6%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 19,105 (34.5%) Total basepairs processed: 14,454,675 bp Total written (filtered): 5,073,010 bp (35.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19135 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 4 18 13 0.0 3 0 0 12 1 19 319 0.0 3 0 274 28 10 7 20 17295 0.0 4 16231 862 149 44 9 21 37 0.0 4 5 29 1 1 1 22 4 0.0 4 1 1 2 23 75 0.0 4 0 1 0 49 25 30 3 0.0 4 0 0 0 0 3 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 1 84 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 1 109 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 18 0.0 4 0 0 0 0 18 127 1320 0.0 4 0 0 0 0 1320 128 3 0.0 4 0 0 0 0 3 129 1 0.0 4 0 0 0 0 1 130 8 0.0 4 0 0 0 0 8 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 19,105 Reads with adapters: 3,527 (18.5%) Reads written (passing filters): 19,105 (100.0%) Total basepairs processed: 5,073,010 bp Total written (filtered): 5,034,500 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3527 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 8.1% G: 86.7% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 35 298.5 0 35 4 9 74.6 0 9 9 26 0.1 0 2 24 10 497 0.0 1 36 461 11 2923 0.0 1 98 2825 12 24 0.0 1 0 24 13 6 0.0 1 0 6 14 4 0.0 1 0 4 151 1 0.0 1 0 1 170 1 0.0 1 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.