This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.74 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 56,523 (2.3%) Reads that were too short: 5,935 (0.2%) Reads written (passing filters): 50,588 (2.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 13,778,183 bp (1.9%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 56523 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 613 596.3 0 613 7 17878 596.3 0 17878 8 21767 596.3 0 21767 9 39 596.3 0 39 10 26 596.3 0 26 11 23 596.3 0 23 12 25 596.3 0 25 13 19 596.3 0 19 14 25 596.3 0 25 15 19 596.3 0 19 16 23 596.3 0 23 17 16 596.3 0 16 18 12 596.3 0 12 19 17 596.3 0 17 20 15 596.3 0 15 21 17 596.3 0 17 22 27 596.3 0 27 23 28 596.3 0 28 24 21 596.3 0 21 25 24 596.3 0 24 26 17 596.3 0 17 27 17 596.3 0 17 28 17 596.3 0 17 29 26 596.3 0 26 30 24 596.3 0 24 31 15 596.3 0 15 32 22 596.3 0 22 33 10 596.3 0 10 34 21 596.3 0 21 35 20 596.3 0 20 36 29 596.3 0 29 37 17 596.3 0 17 38 24 596.3 0 24 39 21 596.3 0 21 40 27 596.3 0 27 41 30 596.3 0 30 42 24 596.3 0 24 43 31 596.3 0 31 44 14 596.3 0 14 45 21 596.3 0 21 46 22 596.3 0 22 47 20 596.3 0 20 48 26 596.3 0 26 49 35 596.3 0 35 50 17 596.3 0 17 51 28 596.3 0 28 52 28 596.3 0 28 53 16 596.3 0 16 54 20 596.3 0 20 55 36 596.3 0 36 56 35 596.3 0 35 57 36 596.3 0 36 58 22 596.3 0 22 59 28 596.3 0 28 60 41 596.3 0 41 61 33 596.3 0 33 62 21 596.3 0 21 63 26 596.3 0 26 64 19 596.3 0 19 65 20 596.3 0 20 66 31 596.3 0 31 67 31 596.3 0 31 68 39 596.3 0 39 69 159 596.3 0 159 70 301 596.3 0 301 71 219 596.3 0 219 72 561 596.3 0 561 73 780 596.3 0 780 74 385 596.3 0 385 75 26 596.3 0 26 76 22 596.3 0 22 77 20 596.3 0 20 78 19 596.3 0 19 79 35 596.3 0 35 80 44 596.3 0 44 81 73 596.3 0 73 82 117 596.3 0 117 83 63 596.3 0 63 84 278 596.3 0 278 85 123 596.3 0 123 86 364 596.3 0 364 87 22 596.3 0 22 88 19 596.3 0 19 89 20 596.3 0 20 90 21 596.3 0 21 91 30 596.3 0 30 92 17 596.3 0 17 93 10 596.3 0 10 94 34 596.3 0 34 95 10 596.3 0 10 96 14 596.3 0 14 97 33 596.3 0 33 98 24 596.3 0 24 99 25 596.3 0 25 100 33 596.3 0 33 101 23 596.3 0 23 102 16 596.3 0 16 103 24 596.3 0 24 104 12 596.3 0 12 105 19 596.3 0 19 106 30 596.3 0 30 107 20 596.3 0 20 108 21 596.3 0 21 109 19 596.3 0 19 110 33 596.3 0 33 111 227 596.3 0 227 112 308 596.3 0 308 113 21 596.3 0 21 114 17 596.3 0 17 115 35 596.3 0 35 116 16 596.3 0 16 117 26 596.3 0 26 118 13 596.3 0 13 119 27 596.3 0 27 120 17 596.3 0 17 121 20 596.3 0 20 122 14 596.3 0 14 123 23 596.3 0 23 124 39 596.3 0 39 125 16 596.3 0 16 126 32 596.3 0 32 127 16 596.3 0 16 128 24 596.3 0 24 129 29 596.3 0 29 130 18 596.3 0 18 131 29 596.3 0 29 132 36 596.3 0 36 133 25 596.3 0 25 134 21 596.3 0 21 135 19 596.3 0 19 136 21 596.3 0 21 137 15 596.3 0 15 138 29 596.3 0 29 139 20 596.3 0 20 140 27 596.3 0 27 141 14 596.3 0 14 142 26 596.3 0 26 143 205 596.3 0 205 144 31 596.3 0 31 145 44 596.3 0 44 146 39 596.3 0 39 147 41 596.3 0 41 148 157 596.3 0 157 149 109 596.3 0 109 150 247 596.3 0 247 151 29 596.3 0 29 152 30 596.3 0 30 153 15 596.3 0 15 154 20 596.3 0 20 155 26 596.3 0 26 156 31 596.3 0 31 157 64 596.3 0 64 158 55 596.3 0 55 159 21 596.3 0 21 160 19 596.3 0 19 161 32 596.3 0 32 162 24 596.3 0 24 163 17 596.3 0 17 164 22 596.3 0 22 165 30 596.3 0 30 166 19 596.3 0 19 167 27 596.3 0 27 168 143 596.3 0 143 169 74 596.3 0 74 170 103 596.3 0 103 171 26 596.3 0 26 172 32 596.3 0 32 173 18 596.3 0 18 174 22 596.3 0 22 175 26 596.3 0 26 176 79 596.3 0 79 177 78 596.3 0 78 178 102 596.3 0 102 179 20 596.3 0 20 180 22 596.3 0 22 181 25 596.3 0 25 182 26 596.3 0 26 183 44 596.3 0 44 184 119 596.3 0 119 185 18 596.3 0 18 186 35 596.3 0 35 187 46 596.3 0 46 188 54 596.3 0 54 189 60 596.3 0 60 190 39 596.3 0 39 191 33 596.3 0 33 192 27 596.3 0 27 193 21 596.3 0 21 194 37 596.3 0 37 195 44 596.3 0 44 196 66 596.3 0 66 197 131 596.3 0 131 198 235 596.3 0 235 199 291 596.3 0 291 200 85 596.3 0 85 201 24 596.3 0 24 202 81 596.3 0 81 203 160 596.3 0 160 204 209 596.3 0 209 205 132 596.3 0 132 206 78 596.3 0 78 207 21 596.3 0 21 208 33 596.3 0 33 209 30 596.3 0 30 210 30 596.3 0 30 211 37 596.3 0 37 212 25 596.3 0 25 213 55 596.3 0 55 214 57 596.3 0 57 215 61 596.3 0 61 216 48 596.3 0 48 217 38 596.3 0 38 218 44 596.3 0 44 219 39 596.3 0 39 220 28 596.3 0 28 221 32 596.3 0 32 222 59 596.3 0 59 223 320 596.3 0 320 224 318 596.3 0 318 225 242 596.3 0 242 226 22 596.3 0 22 227 18 596.3 0 18 228 31 596.3 0 31 229 22 596.3 0 22 230 22 596.3 0 22 231 21 596.3 0 21 232 17 596.3 0 17 233 19 596.3 0 19 234 35 596.3 0 35 235 31 596.3 0 31 236 45 596.3 0 45 237 33 596.3 0 33 238 35 596.3 0 35 239 43 596.3 0 43 240 35 596.3 0 35 241 29 596.3 0 29 242 49 596.3 0 49 243 34 596.3 0 34 244 33 596.3 0 33 245 62 596.3 0 62 246 44 596.3 0 44 247 29 596.3 0 29 248 26 596.3 0 26 249 38 596.3 0 38 250 36 596.3 0 36 251 34 596.3 0 34 252 25 596.3 0 25 253 41 596.3 0 41 254 35 596.3 0 35 255 26 596.3 0 26 256 30 596.3 0 30 257 32 596.3 0 32 258 68 596.3 0 68 259 160 596.3 0 160 260 174 596.3 0 174 261 43 596.3 0 43 262 28 596.3 0 28 263 26 596.3 0 26 264 25 596.3 0 25 265 24 596.3 0 24 266 19 596.3 0 19 267 13 596.3 0 13 268 32 596.3 0 32 269 30 596.3 0 30 270 34 596.3 0 34 271 91 596.3 0 91 272 82 596.3 0 82 273 58 596.3 0 58 274 22 596.3 0 22 275 24 596.3 0 24 276 33 596.3 0 33 277 47 596.3 0 47 278 43 596.3 0 43 279 39 596.3 0 39 280 29 596.3 0 29 281 39 596.3 0 39 282 90 596.3 0 90 283 167 596.3 0 167 284 236 596.3 0 236 285 57 596.3 0 57 286 42 596.3 0 42 287 48 596.3 0 48 288 25 596.3 0 25 289 24 596.3 0 24 290 18 596.3 0 18 291 36 596.3 0 36 292 35 596.3 0 35 293 42 596.3 0 42 294 34 596.3 0 34 295 38 596.3 0 38 296 55 596.3 0 55 297 115 596.3 0 115 298 163 596.3 0 163 299 197 596.3 0 197 300 78 596.3 0 78 301 43 596.3 0 43 Finished in 2.48 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 50,588 Reads with adapters: 17,666 (34.9%) Reads that were too short: 161 (0.3%) Reads written (passing filters): 17,505 (34.6%) Total basepairs processed: 13,778,183 bp Total written (filtered): 4,847,231 bp (35.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17666 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 121 0.0 3 0 120 1 17 17304 0.0 3 16901 393 9 1 18 39 0.0 3 3 35 1 19 8 0.0 3 1 0 5 2 20 18 0.0 3 2 0 0 16 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 1 139 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 1 156 9 0.0 3 0 0 0 9 183 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 1 216 5 0.0 3 0 0 0 5 217 118 0.0 3 0 0 3 115 218 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 17,505 Reads with adapters: 39 (0.2%) Reads written (passing filters): 17,505 (100.0%) Total basepairs processed: 4,847,231 bp Total written (filtered): 4,847,107 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 39 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 38.5% C: 48.7% G: 10.3% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 32 273.5 0 32 4 7 68.4 0 7