This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.45 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 294,634 (12.1%) Reads that were too short: 50,116 (2.1%) Reads written (passing filters): 244,518 (10.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 60,895,710 bp (8.3%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 294634 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 8732 596.3 0 8732 7 70328 596.3 0 70328 8 1211 596.3 0 1211 9 85421 596.3 0 85421 10 78 596.3 0 78 11 26 596.3 0 26 12 22 596.3 0 22 13 14 596.3 0 14 14 18 596.3 0 18 15 29 596.3 0 29 16 31 596.3 0 31 17 39 596.3 0 39 18 23 596.3 0 23 19 25 596.3 0 25 20 21 596.3 0 21 21 23 596.3 0 23 22 19 596.3 0 19 23 24 596.3 0 24 24 23 596.3 0 23 25 16 596.3 0 16 26 30 596.3 0 30 27 23 596.3 0 23 28 29 596.3 0 29 29 23 596.3 0 23 30 34 596.3 0 34 31 60 596.3 0 60 32 22 596.3 0 22 33 23 596.3 0 23 34 25 596.3 0 25 35 16 596.3 0 16 36 60 596.3 0 60 37 32 596.3 0 32 38 30 596.3 0 30 39 52 596.3 0 52 40 23 596.3 0 23 41 17 596.3 0 17 42 27 596.3 0 27 43 26 596.3 0 26 44 20 596.3 0 20 45 23 596.3 0 23 46 20 596.3 0 20 47 40 596.3 0 40 48 35 596.3 0 35 49 21 596.3 0 21 50 24 596.3 0 24 51 19 596.3 0 19 52 18 596.3 0 18 53 34 596.3 0 34 54 30 596.3 0 30 55 32 596.3 0 32 56 64 596.3 0 64 57 87 596.3 0 87 58 236 596.3 0 236 59 30 596.3 0 30 60 26 596.3 0 26 61 24 596.3 0 24 62 32 596.3 0 32 63 32 596.3 0 32 64 22 596.3 0 22 65 21 596.3 0 21 66 32 596.3 0 32 67 33 596.3 0 33 68 24 596.3 0 24 69 26 596.3 0 26 70 35 596.3 0 35 71 90 596.3 0 90 72 60 596.3 0 60 73 150 596.3 0 150 74 24 596.3 0 24 75 28 596.3 0 28 76 22 596.3 0 22 77 23 596.3 0 23 78 37 596.3 0 37 79 41 596.3 0 41 80 18 596.3 0 18 81 28 596.3 0 28 82 33 596.3 0 33 83 44 596.3 0 44 84 40 596.3 0 40 85 48 596.3 0 48 86 65 596.3 0 65 87 111 596.3 0 111 88 70 596.3 0 70 89 106 596.3 0 106 90 396 596.3 0 396 91 150 596.3 0 150 92 28 596.3 0 28 93 29 596.3 0 29 94 22 596.3 0 22 95 25 596.3 0 25 96 25 596.3 0 25 97 24 596.3 0 24 98 38 596.3 0 38 99 39 596.3 0 39 100 31 596.3 0 31 101 45 596.3 0 45 102 51 596.3 0 51 103 273 596.3 0 273 104 326 596.3 0 326 105 376 596.3 0 376 106 224 596.3 0 224 107 93 596.3 0 93 108 129 596.3 0 129 109 66 596.3 0 66 110 24 596.3 0 24 111 51 596.3 0 51 112 54 596.3 0 54 113 70 596.3 0 70 114 48 596.3 0 48 115 42 596.3 0 42 116 57 596.3 0 57 117 38 596.3 0 38 118 48 596.3 0 48 119 107 596.3 0 107 120 62 596.3 0 62 121 73 596.3 0 73 122 40 596.3 0 40 123 105 596.3 0 105 124 41 596.3 0 41 125 38 596.3 0 38 126 60 596.3 0 60 127 148 596.3 0 148 128 192 596.3 0 192 129 328 596.3 0 328 130 85 596.3 0 85 131 146 596.3 0 146 132 176 596.3 0 176 133 169 596.3 0 169 134 272 596.3 0 272 135 152 596.3 0 152 136 177 596.3 0 177 137 192 596.3 0 192 138 216 596.3 0 216 139 357 596.3 0 357 140 430 596.3 0 430 141 502 596.3 0 502 142 483 596.3 0 483 143 601 596.3 0 601 144 561 596.3 0 561 145 899 596.3 0 899 146 9702 596.3 0 9702 147 8168 596.3 0 8168 148 15970 596.3 0 15970 149 632 596.3 0 632 150 7991 596.3 0 7991 151 3460 596.3 0 3460 152 14439 596.3 0 14439 153 100 596.3 0 100 154 72 596.3 0 72 155 94 596.3 0 94 156 472 596.3 0 472 157 327 596.3 0 327 158 428 596.3 0 428 159 398 596.3 0 398 160 226 596.3 0 226 161 260 596.3 0 260 162 227 596.3 0 227 163 68 596.3 0 68 164 49 596.3 0 49 165 52 596.3 0 52 166 82 596.3 0 82 167 64 596.3 0 64 168 61 596.3 0 61 169 45 596.3 0 45 170 59 596.3 0 59 171 36 596.3 0 36 172 31 596.3 0 31 173 42 596.3 0 42 174 48 596.3 0 48 175 189 596.3 0 189 176 431 596.3 0 431 177 124 596.3 0 124 178 45 596.3 0 45 179 57 596.3 0 57 180 56 596.3 0 56 181 150 596.3 0 150 182 134 596.3 0 134 183 145 596.3 0 145 184 120 596.3 0 120 185 157 596.3 0 157 186 228 596.3 0 228 187 124 596.3 0 124 188 106 596.3 0 106 189 53 596.3 0 53 190 37 596.3 0 37 191 37 596.3 0 37 192 44 596.3 0 44 193 33 596.3 0 33 194 42 596.3 0 42 195 66 596.3 0 66 196 47 596.3 0 47 197 36 596.3 0 36 198 33 596.3 0 33 199 87 596.3 0 87 200 77 596.3 0 77 201 70 596.3 0 70 202 76 596.3 0 76 203 102 596.3 0 102 204 170 596.3 0 170 205 138 596.3 0 138 206 121 596.3 0 121 207 100 596.3 0 100 208 221 596.3 0 221 209 119 596.3 0 119 210 102 596.3 0 102 211 97 596.3 0 97 212 278 596.3 0 278 213 186 596.3 0 186 214 177 596.3 0 177 215 77 596.3 0 77 216 72 596.3 0 72 217 40 596.3 0 40 218 55 596.3 0 55 219 92 596.3 0 92 220 232 596.3 0 232 221 166 596.3 0 166 222 237 596.3 0 237 223 73 596.3 0 73 224 103 596.3 0 103 225 189 596.3 0 189 226 112 596.3 0 112 227 63 596.3 0 63 228 67 596.3 0 67 229 88 596.3 0 88 230 75 596.3 0 75 231 79 596.3 0 79 232 81 596.3 0 81 233 78 596.3 0 78 234 62 596.3 0 62 235 132 596.3 0 132 236 150 596.3 0 150 237 101 596.3 0 101 238 144 596.3 0 144 239 136 596.3 0 136 240 88 596.3 0 88 241 120 596.3 0 120 242 111 596.3 0 111 243 138 596.3 0 138 244 185 596.3 0 185 245 151 596.3 0 151 246 120 596.3 0 120 247 86 596.3 0 86 248 77 596.3 0 77 249 130 596.3 0 130 250 135 596.3 0 135 251 179 596.3 0 179 252 256 596.3 0 256 253 296 596.3 0 296 254 498 596.3 0 498 255 998 596.3 0 998 256 9518 596.3 0 9518 257 8732 596.3 0 8732 258 13507 596.3 0 13507 259 3314 596.3 0 3314 260 205 596.3 0 205 261 231 596.3 0 231 262 334 596.3 0 334 263 268 596.3 0 268 264 192 596.3 0 192 265 87 596.3 0 87 266 138 596.3 0 138 267 219 596.3 0 219 268 221 596.3 0 221 269 168 596.3 0 168 270 66 596.3 0 66 271 64 596.3 0 64 272 66 596.3 0 66 273 95 596.3 0 95 274 172 596.3 0 172 275 163 596.3 0 163 276 102 596.3 0 102 277 90 596.3 0 90 278 73 596.3 0 73 279 137 596.3 0 137 280 352 596.3 0 352 281 523 596.3 0 523 282 397 596.3 0 397 283 247 596.3 0 247 284 295 596.3 0 295 285 293 596.3 0 293 286 129 596.3 0 129 287 93 596.3 0 93 288 97 596.3 0 97 289 185 596.3 0 185 290 128 596.3 0 128 291 67 596.3 0 67 292 68 596.3 0 68 293 117 596.3 0 117 294 91 596.3 0 91 295 89 596.3 0 89 296 118 596.3 0 118 297 101 596.3 0 101 298 98 596.3 0 98 299 79 596.3 0 79 300 80 596.3 0 80 301 148 596.3 0 148 Finished in 11.41 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 244,518 Reads with adapters: 79,731 (32.6%) Reads that were too short: 58 (0.0%) Reads written (passing filters): 79,673 (32.6%) Total basepairs processed: 60,895,710 bp Total written (filtered): 21,622,661 bp (35.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 79731 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 1 4 18 33 0.0 3 0 0 24 7 2 19 864 0.0 3 0 735 89 30 10 20 76440 0.0 4 71523 3913 742 203 59 21 191 0.0 4 30 138 9 6 8 22 17 0.0 4 1 1 9 1 5 23 143 0.0 4 5 0 1 97 40 24 4 0.0 4 1 0 0 0 3 26 1 0.0 4 0 1 28 3 0.0 4 0 0 0 0 3 30 1 0.0 4 0 0 0 0 1 34 3 0.0 4 0 0 0 0 3 35 1 0.0 4 0 1 38 2 0.0 4 1 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 1 60 2 0.0 4 0 0 0 1 1 62 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 1 73 1 0.0 4 1 74 1 0.0 4 1 75 2 0.0 4 2 84 2 0.0 4 0 0 0 0 2 91 3 0.0 4 0 0 0 0 3 92 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 120 1 0.0 4 1 123 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 41 0.0 4 0 0 0 0 41 127 1890 0.0 4 0 0 0 0 1890 128 3 0.0 4 0 0 0 0 3 129 1 0.0 4 0 0 0 0 1 130 5 0.0 4 0 0 0 0 5 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 8 0.0 4 0 0 0 2 6 190 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 7 0.0 4 0 0 0 0 7 230 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 241 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 260 2 0.0 4 0 0 0 0 2 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 3 0.0 4 0 0 0 1 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.78 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 79,673 Reads with adapters: 5,962 (7.5%) Reads written (passing filters): 79,673 (100.0%) Total basepairs processed: 21,622,661 bp Total written (filtered): 21,465,115 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5962 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.5% C: 12.4% G: 74.1% T: 5.2% none/other: 2.9% Overview of removed sequences length count expect max.err error counts 3 341 1244.9 0 341 4 49 311.2 0 49 5 2 77.8 0 2 6 1 19.5 0 1 7 2 4.9 0 2 8 1 1.2 0 1 9 24 0.3 0 1 23 10 59 0.1 1 1 58 11 3761 0.0 1 170 3591 12 1247 0.0 1 33 1214 13 90 0.0 1 2 88 14 5 0.0 1 0 5 15 4 0.0 1 0 4 16 5 0.0 1 0 5 44 1 0.0 1 1 136 1 0.0 1 1 139 1 0.0 1 1 152 9 0.0 1 7 2 153 2 0.0 1 1 1 162 1 0.0 1 1 165 1 0.0 1 1 171 1 0.0 1 1 209 1 0.0 1 1 210 13 0.0 1 13 211 2 0.0 1 1 1 225 9 0.0 1 8 1 263 3 0.0 1 3 264 2 0.0 1 2 265 1 0.0 1 1 271 28 0.0 1 25 3 272 6 0.0 1 5 1 273 112 0.0 1 105 7 274 162 0.0 1 150 12 275 15 0.0 1 15