This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.73 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 285,388 (11.7%) Reads that were too short: 26,280 (1.1%) Reads written (passing filters): 259,108 (10.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 64,038,010 bp (8.7%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 285388 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9390 596.3 0 9390 7 85480 596.3 0 85480 8 836 596.3 0 836 9 76759 596.3 0 76759 10 45 596.3 0 45 11 21 596.3 0 21 12 30 596.3 0 30 13 12 596.3 0 12 14 15 596.3 0 15 15 13 596.3 0 13 16 23 596.3 0 23 17 31 596.3 0 31 18 23 596.3 0 23 19 23 596.3 0 23 20 12 596.3 0 12 21 20 596.3 0 20 22 11 596.3 0 11 23 21 596.3 0 21 24 26 596.3 0 26 25 23 596.3 0 23 26 26 596.3 0 26 27 15 596.3 0 15 28 20 596.3 0 20 29 22 596.3 0 22 30 22 596.3 0 22 31 30 596.3 0 30 32 22 596.3 0 22 33 18 596.3 0 18 34 25 596.3 0 25 35 17 596.3 0 17 36 22 596.3 0 22 37 23 596.3 0 23 38 26 596.3 0 26 39 23 596.3 0 23 40 19 596.3 0 19 41 24 596.3 0 24 42 17 596.3 0 17 43 25 596.3 0 25 44 18 596.3 0 18 45 14 596.3 0 14 46 15 596.3 0 15 47 29 596.3 0 29 48 11 596.3 0 11 49 7 596.3 0 7 50 19 596.3 0 19 51 26 596.3 0 26 52 25 596.3 0 25 53 20 596.3 0 20 54 25 596.3 0 25 55 18 596.3 0 18 56 58 596.3 0 58 57 56 596.3 0 56 58 199 596.3 0 199 59 23 596.3 0 23 60 20 596.3 0 20 61 17 596.3 0 17 62 27 596.3 0 27 63 23 596.3 0 23 64 15 596.3 0 15 65 21 596.3 0 21 66 24 596.3 0 24 67 29 596.3 0 29 68 34 596.3 0 34 69 23 596.3 0 23 70 22 596.3 0 22 71 42 596.3 0 42 72 36 596.3 0 36 73 81 596.3 0 81 74 19 596.3 0 19 75 25 596.3 0 25 76 20 596.3 0 20 77 21 596.3 0 21 78 24 596.3 0 24 79 28 596.3 0 28 80 14 596.3 0 14 81 23 596.3 0 23 82 34 596.3 0 34 83 33 596.3 0 33 84 24 596.3 0 24 85 41 596.3 0 41 86 34 596.3 0 34 87 104 596.3 0 104 88 59 596.3 0 59 89 123 596.3 0 123 90 292 596.3 0 292 91 153 596.3 0 153 92 40 596.3 0 40 93 30 596.3 0 30 94 37 596.3 0 37 95 30 596.3 0 30 96 28 596.3 0 28 97 32 596.3 0 32 98 29 596.3 0 29 99 27 596.3 0 27 100 35 596.3 0 35 101 37 596.3 0 37 102 61 596.3 0 61 103 168 596.3 0 168 104 193 596.3 0 193 105 178 596.3 0 178 106 138 596.3 0 138 107 64 596.3 0 64 108 63 596.3 0 63 109 54 596.3 0 54 110 25 596.3 0 25 111 36 596.3 0 36 112 37 596.3 0 37 113 51 596.3 0 51 114 29 596.3 0 29 115 40 596.3 0 40 116 67 596.3 0 67 117 32 596.3 0 32 118 52 596.3 0 52 119 80 596.3 0 80 120 45 596.3 0 45 121 40 596.3 0 40 122 53 596.3 0 53 123 102 596.3 0 102 124 36 596.3 0 36 125 35 596.3 0 35 126 43 596.3 0 43 127 78 596.3 0 78 128 118 596.3 0 118 129 253 596.3 0 253 130 62 596.3 0 62 131 131 596.3 0 131 132 154 596.3 0 154 133 189 596.3 0 189 134 273 596.3 0 273 135 111 596.3 0 111 136 132 596.3 0 132 137 149 596.3 0 149 138 160 596.3 0 160 139 248 596.3 0 248 140 293 596.3 0 293 141 322 596.3 0 322 142 384 596.3 0 384 143 642 596.3 0 642 144 560 596.3 0 560 145 929 596.3 0 929 146 12004 596.3 0 12004 147 10044 596.3 0 10044 148 19334 596.3 0 19334 149 677 596.3 0 677 150 9426 596.3 0 9426 151 3872 596.3 0 3872 152 17683 596.3 0 17683 153 100 596.3 0 100 154 60 596.3 0 60 155 63 596.3 0 63 156 388 596.3 0 388 157 244 596.3 0 244 158 255 596.3 0 255 159 264 596.3 0 264 160 151 596.3 0 151 161 141 596.3 0 141 162 128 596.3 0 128 163 48 596.3 0 48 164 34 596.3 0 34 165 32 596.3 0 32 166 40 596.3 0 40 167 40 596.3 0 40 168 47 596.3 0 47 169 39 596.3 0 39 170 34 596.3 0 34 171 25 596.3 0 25 172 24 596.3 0 24 173 31 596.3 0 31 174 33 596.3 0 33 175 92 596.3 0 92 176 141 596.3 0 141 177 57 596.3 0 57 178 27 596.3 0 27 179 31 596.3 0 31 180 40 596.3 0 40 181 63 596.3 0 63 182 64 596.3 0 64 183 47 596.3 0 47 184 62 596.3 0 62 185 64 596.3 0 64 186 105 596.3 0 105 187 65 596.3 0 65 188 58 596.3 0 58 189 38 596.3 0 38 190 36 596.3 0 36 191 25 596.3 0 25 192 30 596.3 0 30 193 26 596.3 0 26 194 31 596.3 0 31 195 47 596.3 0 47 196 30 596.3 0 30 197 25 596.3 0 25 198 26 596.3 0 26 199 37 596.3 0 37 200 36 596.3 0 36 201 51 596.3 0 51 202 67 596.3 0 67 203 67 596.3 0 67 204 65 596.3 0 65 205 58 596.3 0 58 206 48 596.3 0 48 207 51 596.3 0 51 208 127 596.3 0 127 209 71 596.3 0 71 210 53 596.3 0 53 211 57 596.3 0 57 212 223 596.3 0 223 213 99 596.3 0 99 214 90 596.3 0 90 215 49 596.3 0 49 216 54 596.3 0 54 217 49 596.3 0 49 218 34 596.3 0 34 219 61 596.3 0 61 220 135 596.3 0 135 221 100 596.3 0 100 222 121 596.3 0 121 223 60 596.3 0 60 224 71 596.3 0 71 225 145 596.3 0 145 226 72 596.3 0 72 227 41 596.3 0 41 228 46 596.3 0 46 229 53 596.3 0 53 230 67 596.3 0 67 231 56 596.3 0 56 232 56 596.3 0 56 233 69 596.3 0 69 234 66 596.3 0 66 235 90 596.3 0 90 236 92 596.3 0 92 237 65 596.3 0 65 238 94 596.3 0 94 239 92 596.3 0 92 240 73 596.3 0 73 241 80 596.3 0 80 242 59 596.3 0 59 243 78 596.3 0 78 244 92 596.3 0 92 245 68 596.3 0 68 246 66 596.3 0 66 247 50 596.3 0 50 248 60 596.3 0 60 249 83 596.3 0 83 250 89 596.3 0 89 251 114 596.3 0 114 252 145 596.3 0 145 253 142 596.3 0 142 254 247 596.3 0 247 255 461 596.3 0 461 256 4192 596.3 0 4192 257 4044 596.3 0 4044 258 7286 596.3 0 7286 259 1999 596.3 0 1999 260 105 596.3 0 105 261 101 596.3 0 101 262 170 596.3 0 170 263 126 596.3 0 126 264 94 596.3 0 94 265 73 596.3 0 73 266 68 596.3 0 68 267 78 596.3 0 78 268 114 596.3 0 114 269 75 596.3 0 75 270 37 596.3 0 37 271 57 596.3 0 57 272 42 596.3 0 42 273 46 596.3 0 46 274 94 596.3 0 94 275 118 596.3 0 118 276 59 596.3 0 59 277 58 596.3 0 58 278 48 596.3 0 48 279 95 596.3 0 95 280 218 596.3 0 218 281 299 596.3 0 299 282 253 596.3 0 253 283 142 596.3 0 142 284 93 596.3 0 93 285 121 596.3 0 121 286 60 596.3 0 60 287 78 596.3 0 78 288 74 596.3 0 74 289 132 596.3 0 132 290 90 596.3 0 90 291 27 596.3 0 27 292 51 596.3 0 51 293 79 596.3 0 79 294 70 596.3 0 70 295 96 596.3 0 96 296 94 596.3 0 94 297 54 596.3 0 54 298 70 596.3 0 70 299 56 596.3 0 56 300 50 596.3 0 50 301 73 596.3 0 73 Finished in 10.60 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 259,108 Reads with adapters: 78,071 (30.1%) Reads that were too short: 261 (0.1%) Reads written (passing filters): 77,810 (30.0%) Total basepairs processed: 64,038,010 bp Total written (filtered): 21,396,190 bp (33.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 78071 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 9 0.0 3 0 0 8 1 16 589 0.0 3 0 567 21 1 17 76919 0.0 3 75131 1720 61 7 18 130 0.0 3 8 112 9 1 19 68 0.0 3 0 1 52 15 20 65 0.0 3 0 0 0 65 25 1 0.0 3 0 1 33 5 0.0 3 0 0 0 5 36 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 1 71 1 0.0 3 0 1 72 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 5 0.0 3 0 1 0 4 133 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 163 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 183 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 216 5 0.0 3 0 0 0 5 217 228 0.0 3 0 0 6 222 218 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.78 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 77,810 Reads with adapters: 3,823 (4.9%) Reads written (passing filters): 77,810 (100.0%) Total basepairs processed: 21,396,190 bp Total written (filtered): 21,384,587 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3823 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.4% C: 6.9% G: 10.5% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3691 1215.8 0 3691 4 130 303.9 0 130 5 2 76.0 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.