This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.67 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 118,655 (4.9%) Reads that were too short: 21,139 (0.9%) Reads written (passing filters): 97,516 (4.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 21,061,578 bp (2.9%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 118655 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1969 596.3 0 1969 7 22444 596.3 0 22444 8 932 596.3 0 932 9 19723 596.3 0 19723 10 37 596.3 0 37 11 49 596.3 0 49 12 14 596.3 0 14 13 27 596.3 0 27 14 9 596.3 0 9 15 30 596.3 0 30 16 15 596.3 0 15 17 16 596.3 0 16 18 17 596.3 0 17 19 15 596.3 0 15 20 13 596.3 0 13 21 18 596.3 0 18 22 18 596.3 0 18 23 30 596.3 0 30 24 20 596.3 0 20 25 20 596.3 0 20 26 21 596.3 0 21 27 20 596.3 0 20 28 14 596.3 0 14 29 16 596.3 0 16 30 35 596.3 0 35 31 12 596.3 0 12 32 15 596.3 0 15 33 18 596.3 0 18 34 15 596.3 0 15 35 20 596.3 0 20 36 17 596.3 0 17 37 13 596.3 0 13 38 28 596.3 0 28 39 18 596.3 0 18 40 126 596.3 0 126 41 20 596.3 0 20 42 74 596.3 0 74 43 19 596.3 0 19 44 15 596.3 0 15 45 15 596.3 0 15 46 27 596.3 0 27 47 17 596.3 0 17 48 10 596.3 0 10 49 27 596.3 0 27 50 28 596.3 0 28 51 16 596.3 0 16 52 16 596.3 0 16 53 23 596.3 0 23 54 19 596.3 0 19 55 116 596.3 0 116 56 61 596.3 0 61 57 147 596.3 0 147 58 30 596.3 0 30 59 35 596.3 0 35 60 39 596.3 0 39 61 38 596.3 0 38 62 25 596.3 0 25 63 9 596.3 0 9 64 39 596.3 0 39 65 27 596.3 0 27 66 44 596.3 0 44 67 26 596.3 0 26 68 54 596.3 0 54 69 20 596.3 0 20 70 20 596.3 0 20 71 45 596.3 0 45 72 30 596.3 0 30 73 30 596.3 0 30 74 12 596.3 0 12 75 87 596.3 0 87 76 20 596.3 0 20 77 22 596.3 0 22 78 21 596.3 0 21 79 19 596.3 0 19 80 15 596.3 0 15 81 13 596.3 0 13 82 25 596.3 0 25 83 32 596.3 0 32 84 63 596.3 0 63 85 33 596.3 0 33 86 29 596.3 0 29 87 27 596.3 0 27 88 31 596.3 0 31 89 40 596.3 0 40 90 51 596.3 0 51 91 57 596.3 0 57 92 43 596.3 0 43 93 34 596.3 0 34 94 26 596.3 0 26 95 34 596.3 0 34 96 17 596.3 0 17 97 26 596.3 0 26 98 23 596.3 0 23 99 8 596.3 0 8 100 19 596.3 0 19 101 28 596.3 0 28 102 29 596.3 0 29 103 66 596.3 0 66 104 60 596.3 0 60 105 306 596.3 0 306 106 291 596.3 0 291 107 147 596.3 0 147 108 21 596.3 0 21 109 21 596.3 0 21 110 31 596.3 0 31 111 32 596.3 0 32 112 24 596.3 0 24 113 28 596.3 0 28 114 50 596.3 0 50 115 158 596.3 0 158 116 30 596.3 0 30 117 35 596.3 0 35 118 30 596.3 0 30 119 17 596.3 0 17 120 25 596.3 0 25 121 35 596.3 0 35 122 28 596.3 0 28 123 58 596.3 0 58 124 28 596.3 0 28 125 47 596.3 0 47 126 61 596.3 0 61 127 316 596.3 0 316 128 55 596.3 0 55 129 188 596.3 0 188 130 96 596.3 0 96 131 48 596.3 0 48 132 135 596.3 0 135 133 137 596.3 0 137 134 211 596.3 0 211 135 80 596.3 0 80 136 85 596.3 0 85 137 183 596.3 0 183 138 79 596.3 0 79 139 88 596.3 0 88 140 146 596.3 0 146 141 128 596.3 0 128 142 248 596.3 0 248 143 193 596.3 0 193 144 242 596.3 0 242 145 322 596.3 0 322 146 442 596.3 0 442 147 7646 596.3 0 7646 148 11771 596.3 0 11771 149 11365 596.3 0 11365 150 117 596.3 0 117 151 80 596.3 0 80 152 192 596.3 0 192 153 204 596.3 0 204 154 391 596.3 0 391 155 296 596.3 0 296 156 84 596.3 0 84 157 64 596.3 0 64 158 87 596.3 0 87 159 68 596.3 0 68 160 30 596.3 0 30 161 42 596.3 0 42 162 38 596.3 0 38 163 54 596.3 0 54 164 53 596.3 0 53 165 42 596.3 0 42 166 38 596.3 0 38 167 104 596.3 0 104 168 426 596.3 0 426 169 100 596.3 0 100 170 200 596.3 0 200 171 58 596.3 0 58 172 114 596.3 0 114 173 195 596.3 0 195 174 165 596.3 0 165 175 128 596.3 0 128 176 94 596.3 0 94 177 72 596.3 0 72 178 129 596.3 0 129 179 102 596.3 0 102 180 37 596.3 0 37 181 38 596.3 0 38 182 59 596.3 0 59 183 67 596.3 0 67 184 99 596.3 0 99 185 69 596.3 0 69 186 94 596.3 0 94 187 182 596.3 0 182 188 1876 596.3 0 1876 189 975 596.3 0 975 190 2634 596.3 0 2634 191 94 596.3 0 94 192 37 596.3 0 37 193 30 596.3 0 30 194 44 596.3 0 44 195 73 596.3 0 73 196 130 596.3 0 130 197 112 596.3 0 112 198 122 596.3 0 122 199 197 596.3 0 197 200 293 596.3 0 293 201 2805 596.3 0 2805 202 1878 596.3 0 1878 203 2959 596.3 0 2959 204 600 596.3 0 600 205 94 596.3 0 94 206 121 596.3 0 121 207 79 596.3 0 79 208 57 596.3 0 57 209 44 596.3 0 44 210 42 596.3 0 42 211 15 596.3 0 15 212 21 596.3 0 21 213 24 596.3 0 24 214 55 596.3 0 55 215 87 596.3 0 87 216 53 596.3 0 53 217 58 596.3 0 58 218 22 596.3 0 22 219 25 596.3 0 25 220 47 596.3 0 47 221 50 596.3 0 50 222 32 596.3 0 32 223 44 596.3 0 44 224 73 596.3 0 73 225 127 596.3 0 127 226 107 596.3 0 107 227 102 596.3 0 102 228 321 596.3 0 321 229 167 596.3 0 167 230 475 596.3 0 475 231 94 596.3 0 94 232 58 596.3 0 58 233 37 596.3 0 37 234 79 596.3 0 79 235 91 596.3 0 91 236 87 596.3 0 87 237 43 596.3 0 43 238 65 596.3 0 65 239 42 596.3 0 42 240 73 596.3 0 73 241 108 596.3 0 108 242 38 596.3 0 38 243 102 596.3 0 102 244 99 596.3 0 99 245 119 596.3 0 119 246 49 596.3 0 49 247 80 596.3 0 80 248 93 596.3 0 93 249 154 596.3 0 154 250 175 596.3 0 175 251 129 596.3 0 129 252 121 596.3 0 121 253 94 596.3 0 94 254 59 596.3 0 59 255 30 596.3 0 30 256 45 596.3 0 45 257 38 596.3 0 38 258 88 596.3 0 88 259 118 596.3 0 118 260 127 596.3 0 127 261 101 596.3 0 101 262 110 596.3 0 110 263 87 596.3 0 87 264 83 596.3 0 83 265 64 596.3 0 64 266 72 596.3 0 72 267 54 596.3 0 54 268 71 596.3 0 71 269 422 596.3 0 422 270 468 596.3 0 468 271 463 596.3 0 463 272 173 596.3 0 173 273 73 596.3 0 73 274 93 596.3 0 93 275 196 596.3 0 196 276 177 596.3 0 177 277 150 596.3 0 150 278 95 596.3 0 95 279 101 596.3 0 101 280 206 596.3 0 206 281 286 596.3 0 286 282 500 596.3 0 500 283 337 596.3 0 337 284 216 596.3 0 216 285 164 596.3 0 164 286 302 596.3 0 302 287 539 596.3 0 539 288 353 596.3 0 353 289 107 596.3 0 107 290 108 596.3 0 108 291 347 596.3 0 347 292 496 596.3 0 496 293 777 596.3 0 777 294 1053 596.3 0 1053 295 600 596.3 0 600 296 764 596.3 0 764 297 261 596.3 0 261 298 72 596.3 0 72 299 85 596.3 0 85 300 107 596.3 0 107 301 92 596.3 0 92 Finished in 4.41 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 97,516 Reads with adapters: 21,885 (22.4%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 21,850 (22.4%) Total basepairs processed: 21,061,578 bp Total written (filtered): 5,899,564 bp (28.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21885 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 22 0.0 3 0 0 13 6 3 19 295 0.0 3 0 259 18 9 9 20 20449 0.0 4 19323 915 146 48 17 21 54 0.0 4 17 30 1 3 3 22 481 0.0 4 453 20 6 1 1 23 112 0.0 4 31 1 2 58 20 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 0 0 0 1 26 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 1 42 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 4 0.0 4 0 0 0 0 4 92 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 0 5 127 409 0.0 4 0 0 0 0 409 128 2 0.0 4 0 0 0 0 2 129 3 0.0 4 0 0 0 0 3 130 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 1 162 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 228 10 0.0 4 0 0 0 0 10 229 5 0.0 4 0 0 0 0 5 258 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 21,850 Reads with adapters: 4,906 (22.5%) Reads written (passing filters): 21,850 (100.0%) Total basepairs processed: 5,899,564 bp Total written (filtered): 5,846,605 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4906 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 8.6% G: 87.1% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 341.4 0 12 4 7 85.4 0 7 7 1 1.3 0 1 9 884 0.1 0 92 792 10 3476 0.0 1 226 3250 11 482 0.0 1 27 455 12 14 0.0 1 0 14 13 10 0.0 1 0 10 14 1 0.0 1 0 1 207 1 0.0 1 1 208 4 0.0 1 3 1 209 1 0.0 1 1 223 3 0.0 1 3 238 1 0.0 1 1 267 9 0.0 1 8 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.