This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.26 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 101,250 (4.1%) Reads that were too short: 11,035 (0.5%) Reads written (passing filters): 90,215 (3.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 20,133,036 bp (2.7%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 101250 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1599 596.3 0 1599 7 20163 596.3 0 20163 8 659 596.3 0 659 9 24145 596.3 0 24145 10 41 596.3 0 41 11 13 596.3 0 13 12 4 596.3 0 4 13 10 596.3 0 10 14 5 596.3 0 5 15 13 596.3 0 13 16 6 596.3 0 6 17 6 596.3 0 6 18 6 596.3 0 6 19 9 596.3 0 9 20 13 596.3 0 13 21 11 596.3 0 11 22 11 596.3 0 11 23 8 596.3 0 8 24 7 596.3 0 7 25 7 596.3 0 7 26 5 596.3 0 5 27 8 596.3 0 8 28 7 596.3 0 7 29 8 596.3 0 8 30 12 596.3 0 12 31 12 596.3 0 12 32 13 596.3 0 13 33 12 596.3 0 12 34 7 596.3 0 7 35 7 596.3 0 7 36 8 596.3 0 8 37 15 596.3 0 15 38 20 596.3 0 20 39 9 596.3 0 9 40 24 596.3 0 24 41 13 596.3 0 13 42 9 596.3 0 9 43 7 596.3 0 7 44 13 596.3 0 13 45 13 596.3 0 13 46 17 596.3 0 17 47 9 596.3 0 9 48 7 596.3 0 7 49 10 596.3 0 10 50 8 596.3 0 8 51 8 596.3 0 8 52 5 596.3 0 5 53 12 596.3 0 12 54 7 596.3 0 7 55 68 596.3 0 68 56 31 596.3 0 31 57 73 596.3 0 73 58 12 596.3 0 12 59 16 596.3 0 16 60 12 596.3 0 12 61 9 596.3 0 9 62 12 596.3 0 12 63 12 596.3 0 12 64 10 596.3 0 10 65 20 596.3 0 20 66 8 596.3 0 8 67 9 596.3 0 9 68 13 596.3 0 13 69 11 596.3 0 11 70 15 596.3 0 15 71 12 596.3 0 12 72 11 596.3 0 11 73 12 596.3 0 12 74 12 596.3 0 12 75 61 596.3 0 61 76 22 596.3 0 22 77 19 596.3 0 19 78 12 596.3 0 12 79 14 596.3 0 14 80 11 596.3 0 11 81 18 596.3 0 18 82 10 596.3 0 10 83 14 596.3 0 14 84 19 596.3 0 19 85 14 596.3 0 14 86 31 596.3 0 31 87 15 596.3 0 15 88 20 596.3 0 20 89 26 596.3 0 26 90 22 596.3 0 22 91 19 596.3 0 19 92 16 596.3 0 16 93 16 596.3 0 16 94 10 596.3 0 10 95 16 596.3 0 16 96 22 596.3 0 22 97 19 596.3 0 19 98 13 596.3 0 13 99 11 596.3 0 11 100 10 596.3 0 10 101 9 596.3 0 9 102 23 596.3 0 23 103 46 596.3 0 46 104 24 596.3 0 24 105 92 596.3 0 92 106 123 596.3 0 123 107 67 596.3 0 67 108 13 596.3 0 13 109 16 596.3 0 16 110 8 596.3 0 8 111 10 596.3 0 10 112 18 596.3 0 18 113 23 596.3 0 23 114 18 596.3 0 18 115 120 596.3 0 120 116 18 596.3 0 18 117 23 596.3 0 23 118 22 596.3 0 22 119 17 596.3 0 17 120 15 596.3 0 15 121 18 596.3 0 18 122 17 596.3 0 17 123 41 596.3 0 41 124 14 596.3 0 14 125 27 596.3 0 27 126 31 596.3 0 31 127 269 596.3 0 269 128 37 596.3 0 37 129 170 596.3 0 170 130 64 596.3 0 64 131 52 596.3 0 52 132 91 596.3 0 91 133 94 596.3 0 94 134 192 596.3 0 192 135 79 596.3 0 79 136 69 596.3 0 69 137 139 596.3 0 139 138 73 596.3 0 73 139 70 596.3 0 70 140 159 596.3 0 159 141 101 596.3 0 101 142 169 596.3 0 169 143 173 596.3 0 173 144 216 596.3 0 216 145 221 596.3 0 221 146 311 596.3 0 311 147 7240 596.3 0 7240 148 11423 596.3 0 11423 149 11030 596.3 0 11030 150 90 596.3 0 90 151 46 596.3 0 46 152 96 596.3 0 96 153 63 596.3 0 63 154 116 596.3 0 116 155 112 596.3 0 112 156 48 596.3 0 48 157 23 596.3 0 23 158 33 596.3 0 33 159 25 596.3 0 25 160 20 596.3 0 20 161 14 596.3 0 14 162 16 596.3 0 16 163 17 596.3 0 17 164 26 596.3 0 26 165 17 596.3 0 17 166 30 596.3 0 30 167 73 596.3 0 73 168 396 596.3 0 396 169 69 596.3 0 69 170 143 596.3 0 143 171 35 596.3 0 35 172 50 596.3 0 50 173 67 596.3 0 67 174 107 596.3 0 107 175 50 596.3 0 50 176 65 596.3 0 65 177 35 596.3 0 35 178 51 596.3 0 51 179 43 596.3 0 43 180 19 596.3 0 19 181 25 596.3 0 25 182 31 596.3 0 31 183 38 596.3 0 38 184 71 596.3 0 71 185 35 596.3 0 35 186 55 596.3 0 55 187 91 596.3 0 91 188 1013 596.3 0 1013 189 558 596.3 0 558 190 1558 596.3 0 1558 191 62 596.3 0 62 192 15 596.3 0 15 193 26 596.3 0 26 194 25 596.3 0 25 195 40 596.3 0 40 196 46 596.3 0 46 197 66 596.3 0 66 198 80 596.3 0 80 199 119 596.3 0 119 200 201 596.3 0 201 201 2871 596.3 0 2871 202 1640 596.3 0 1640 203 3715 596.3 0 3715 204 557 596.3 0 557 205 19 596.3 0 19 206 12 596.3 0 12 207 16 596.3 0 16 208 17 596.3 0 17 209 19 596.3 0 19 210 20 596.3 0 20 211 11 596.3 0 11 212 6 596.3 0 6 213 14 596.3 0 14 214 19 596.3 0 19 215 42 596.3 0 42 216 18 596.3 0 18 217 38 596.3 0 38 218 10 596.3 0 10 219 8 596.3 0 8 220 16 596.3 0 16 221 19 596.3 0 19 222 8 596.3 0 8 223 21 596.3 0 21 224 26 596.3 0 26 225 63 596.3 0 63 226 30 596.3 0 30 227 45 596.3 0 45 228 173 596.3 0 173 229 93 596.3 0 93 230 243 596.3 0 243 231 21 596.3 0 21 232 16 596.3 0 16 233 19 596.3 0 19 234 20 596.3 0 20 235 30 596.3 0 30 236 32 596.3 0 32 237 14 596.3 0 14 238 37 596.3 0 37 239 17 596.3 0 17 240 14 596.3 0 14 241 28 596.3 0 28 242 36 596.3 0 36 243 31 596.3 0 31 244 36 596.3 0 36 245 33 596.3 0 33 246 12 596.3 0 12 247 15 596.3 0 15 248 29 596.3 0 29 249 29 596.3 0 29 250 52 596.3 0 52 251 35 596.3 0 35 252 34 596.3 0 34 253 36 596.3 0 36 254 23 596.3 0 23 255 11 596.3 0 11 256 19 596.3 0 19 257 17 596.3 0 17 258 27 596.3 0 27 259 36 596.3 0 36 260 48 596.3 0 48 261 45 596.3 0 45 262 31 596.3 0 31 263 19 596.3 0 19 264 21 596.3 0 21 265 21 596.3 0 21 266 27 596.3 0 27 267 19 596.3 0 19 268 24 596.3 0 24 269 110 596.3 0 110 270 118 596.3 0 118 271 126 596.3 0 126 272 34 596.3 0 34 273 29 596.3 0 29 274 36 596.3 0 36 275 56 596.3 0 56 276 78 596.3 0 78 277 90 596.3 0 90 278 33 596.3 0 33 279 36 596.3 0 36 280 50 596.3 0 50 281 55 596.3 0 55 282 133 596.3 0 133 283 100 596.3 0 100 284 78 596.3 0 78 285 47 596.3 0 47 286 160 596.3 0 160 287 276 596.3 0 276 288 130 596.3 0 130 289 21 596.3 0 21 290 43 596.3 0 43 291 131 596.3 0 131 292 175 596.3 0 175 293 233 596.3 0 233 294 271 596.3 0 271 295 140 596.3 0 140 296 136 596.3 0 136 297 66 596.3 0 66 298 18 596.3 0 18 299 30 596.3 0 30 300 28 596.3 0 28 301 36 596.3 0 36 Finished in 3.73 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 90,215 Reads with adapters: 20,804 (23.1%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 20,772 (23.0%) Total basepairs processed: 20,133,036 bp Total written (filtered): 5,738,630 bp (28.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20804 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 4 1 16 124 0.0 3 0 122 2 17 19968 0.0 3 19537 417 10 4 18 66 0.0 3 12 53 1 19 520 0.0 3 484 10 5 21 20 15 0.0 3 0 1 0 14 21 1 0.0 3 0 0 0 1 22 7 0.0 3 0 0 1 6 27 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 16 0.0 3 0 0 0 16 45 39 0.0 3 0 0 0 39 46 2 0.0 3 0 0 0 2 47 1 0.0 3 1 64 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 79 8 0.0 3 0 0 0 8 86 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 91 3 0.0 3 0 0 0 3 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.73 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 20,772 Reads with adapters: 45 (0.2%) Reads written (passing filters): 20,772 (100.0%) Total basepairs processed: 5,738,630 bp Total written (filtered): 5,738,480 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 45 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.2% C: 42.2% G: 26.7% T: 8.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 32 324.6 0 32 4 11 81.1 0 11 5 2 20.3 0 2