This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.44 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 161,501 (6.6%) Reads that were too short: 46,244 (1.9%) Reads written (passing filters): 115,257 (4.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 29,270,055 bp (4.0%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 161501 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 844 596.3 0 844 7 37359 596.3 0 37359 8 909 596.3 0 909 9 42067 596.3 0 42067 10 41 596.3 0 41 11 14 596.3 0 14 12 17 596.3 0 17 13 19 596.3 0 19 14 9 596.3 0 9 15 13 596.3 0 13 16 16 596.3 0 16 17 33 596.3 0 33 18 7 596.3 0 7 19 14 596.3 0 14 20 10 596.3 0 10 21 10 596.3 0 10 22 10 596.3 0 10 23 11 596.3 0 11 24 11 596.3 0 11 25 16 596.3 0 16 26 21 596.3 0 21 27 22 596.3 0 22 28 27 596.3 0 27 29 15 596.3 0 15 30 23 596.3 0 23 31 14 596.3 0 14 32 24 596.3 0 24 33 12 596.3 0 12 34 13 596.3 0 13 35 18 596.3 0 18 36 16 596.3 0 16 37 16 596.3 0 16 38 21 596.3 0 21 39 19 596.3 0 19 40 28 596.3 0 28 41 139 596.3 0 139 42 223 596.3 0 223 43 283 596.3 0 283 44 19 596.3 0 19 45 20 596.3 0 20 46 13 596.3 0 13 47 15 596.3 0 15 48 14 596.3 0 14 49 10 596.3 0 10 50 27 596.3 0 27 51 36 596.3 0 36 52 19 596.3 0 19 53 9 596.3 0 9 54 12 596.3 0 12 55 3 596.3 0 3 56 13 596.3 0 13 57 27 596.3 0 27 58 11 596.3 0 11 59 22 596.3 0 22 60 11 596.3 0 11 61 13 596.3 0 13 62 30 596.3 0 30 63 21 596.3 0 21 64 21 596.3 0 21 65 22 596.3 0 22 66 29 596.3 0 29 67 32 596.3 0 32 68 12 596.3 0 12 69 80 596.3 0 80 70 19 596.3 0 19 71 69 596.3 0 69 72 108 596.3 0 108 73 86 596.3 0 86 74 257 596.3 0 257 75 103 596.3 0 103 76 78 596.3 0 78 77 83 596.3 0 83 78 58 596.3 0 58 79 58 596.3 0 58 80 64 596.3 0 64 81 40 596.3 0 40 82 106 596.3 0 106 83 52 596.3 0 52 84 47 596.3 0 47 85 23 596.3 0 23 86 19 596.3 0 19 87 26 596.3 0 26 88 21 596.3 0 21 89 15 596.3 0 15 90 67 596.3 0 67 91 32 596.3 0 32 92 40 596.3 0 40 93 34 596.3 0 34 94 47 596.3 0 47 95 47 596.3 0 47 96 41 596.3 0 41 97 48 596.3 0 48 98 57 596.3 0 57 99 39 596.3 0 39 100 36 596.3 0 36 101 60 596.3 0 60 102 88 596.3 0 88 103 110 596.3 0 110 104 225 596.3 0 225 105 239 596.3 0 239 106 255 596.3 0 255 107 250 596.3 0 250 108 206 596.3 0 206 109 187 596.3 0 187 110 123 596.3 0 123 111 164 596.3 0 164 112 157 596.3 0 157 113 204 596.3 0 204 114 161 596.3 0 161 115 139 596.3 0 139 116 184 596.3 0 184 117 129 596.3 0 129 118 213 596.3 0 213 119 200 596.3 0 200 120 262 596.3 0 262 121 168 596.3 0 168 122 181 596.3 0 181 123 186 596.3 0 186 124 530 596.3 0 530 125 202 596.3 0 202 126 651 596.3 0 651 127 184 596.3 0 184 128 280 596.3 0 280 129 172 596.3 0 172 130 192 596.3 0 192 131 236 596.3 0 236 132 322 596.3 0 322 133 2254 596.3 0 2254 134 1529 596.3 0 1529 135 3793 596.3 0 3793 136 168 596.3 0 168 137 158 596.3 0 158 138 120 596.3 0 120 139 152 596.3 0 152 140 138 596.3 0 138 141 131 596.3 0 131 142 138 596.3 0 138 143 122 596.3 0 122 144 175 596.3 0 175 145 218 596.3 0 218 146 134 596.3 0 134 147 193 596.3 0 193 148 151 596.3 0 151 149 127 596.3 0 127 150 113 596.3 0 113 151 116 596.3 0 116 152 133 596.3 0 133 153 85 596.3 0 85 154 141 596.3 0 141 155 138 596.3 0 138 156 180 596.3 0 180 157 233 596.3 0 233 158 210 596.3 0 210 159 387 596.3 0 387 160 414 596.3 0 414 161 479 596.3 0 479 162 1047 596.3 0 1047 163 765 596.3 0 765 164 1739 596.3 0 1739 165 299 596.3 0 299 166 242 596.3 0 242 167 215 596.3 0 215 168 180 596.3 0 180 169 170 596.3 0 170 170 177 596.3 0 177 171 203 596.3 0 203 172 223 596.3 0 223 173 217 596.3 0 217 174 124 596.3 0 124 175 132 596.3 0 132 176 129 596.3 0 129 177 151 596.3 0 151 178 197 596.3 0 197 179 212 596.3 0 212 180 245 596.3 0 245 181 187 596.3 0 187 182 185 596.3 0 185 183 467 596.3 0 467 184 297 596.3 0 297 185 498 596.3 0 498 186 201 596.3 0 201 187 282 596.3 0 282 188 229 596.3 0 229 189 300 596.3 0 300 190 201 596.3 0 201 191 210 596.3 0 210 192 174 596.3 0 174 193 190 596.3 0 190 194 122 596.3 0 122 195 139 596.3 0 139 196 132 596.3 0 132 197 138 596.3 0 138 198 136 596.3 0 136 199 137 596.3 0 137 200 189 596.3 0 189 201 261 596.3 0 261 202 149 596.3 0 149 203 139 596.3 0 139 204 130 596.3 0 130 205 162 596.3 0 162 206 171 596.3 0 171 207 243 596.3 0 243 208 265 596.3 0 265 209 237 596.3 0 237 210 236 596.3 0 236 211 437 596.3 0 437 212 738 596.3 0 738 213 9460 596.3 0 9460 214 2265 596.3 0 2265 215 4384 596.3 0 4384 216 762 596.3 0 762 217 424 596.3 0 424 218 229 596.3 0 229 219 210 596.3 0 210 220 198 596.3 0 198 221 293 596.3 0 293 222 268 596.3 0 268 223 314 596.3 0 314 224 364 596.3 0 364 225 864 596.3 0 864 226 897 596.3 0 897 227 1011 596.3 0 1011 228 435 596.3 0 435 229 409 596.3 0 409 230 261 596.3 0 261 231 216 596.3 0 216 232 177 596.3 0 177 233 127 596.3 0 127 234 169 596.3 0 169 235 171 596.3 0 171 236 197 596.3 0 197 237 266 596.3 0 266 238 169 596.3 0 169 239 183 596.3 0 183 240 148 596.3 0 148 241 155 596.3 0 155 242 173 596.3 0 173 243 154 596.3 0 154 244 125 596.3 0 125 245 136 596.3 0 136 246 154 596.3 0 154 247 178 596.3 0 178 248 241 596.3 0 241 249 292 596.3 0 292 250 546 596.3 0 546 251 453 596.3 0 453 252 873 596.3 0 873 253 367 596.3 0 367 254 494 596.3 0 494 255 453 596.3 0 453 256 288 596.3 0 288 257 205 596.3 0 205 258 202 596.3 0 202 259 179 596.3 0 179 260 139 596.3 0 139 261 177 596.3 0 177 262 203 596.3 0 203 263 361 596.3 0 361 264 373 596.3 0 373 265 542 596.3 0 542 266 389 596.3 0 389 267 225 596.3 0 225 268 260 596.3 0 260 269 278 596.3 0 278 270 399 596.3 0 399 271 326 596.3 0 326 272 256 596.3 0 256 273 259 596.3 0 259 274 286 596.3 0 286 275 207 596.3 0 207 276 311 596.3 0 311 277 333 596.3 0 333 278 400 596.3 0 400 279 294 596.3 0 294 280 192 596.3 0 192 281 269 596.3 0 269 282 272 596.3 0 272 283 278 596.3 0 278 284 266 596.3 0 266 285 326 596.3 0 326 286 292 596.3 0 292 287 256 596.3 0 256 288 260 596.3 0 260 289 393 596.3 0 393 290 361 596.3 0 361 291 366 596.3 0 366 292 242 596.3 0 242 293 231 596.3 0 231 294 301 596.3 0 301 295 342 596.3 0 342 296 272 596.3 0 272 297 236 596.3 0 236 298 228 596.3 0 228 299 237 596.3 0 237 300 312 596.3 0 312 301 848 596.3 0 848 Finished in 5.76 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 115,257 Reads with adapters: 38,245 (33.2%) Reads that were too short: 52 (0.0%) Reads written (passing filters): 38,193 (33.1%) Total basepairs processed: 29,270,055 bp Total written (filtered): 10,383,426 bp (35.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38245 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 20 0.0 3 0 0 18 1 1 19 686 0.0 3 0 622 43 17 4 20 36602 0.0 4 34490 1776 256 60 20 21 68 0.0 4 10 53 3 0 2 22 6 0.0 4 1 0 3 1 1 23 56 0.0 4 0 0 1 44 11 31 1 0.0 4 0 0 0 0 1 33 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 43 1 0.0 4 1 63 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 80 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 1 1 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 1 111 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 126 12 0.0 4 0 0 0 0 12 127 722 0.0 4 0 0 0 0 722 128 3 0.0 4 0 0 0 0 3 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 167 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 2 0.0 4 0 0 0 0 2 189 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 1 265 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 292 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.34 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 38,193 Reads with adapters: 3,141 (8.2%) Reads written (passing filters): 38,193 (100.0%) Total basepairs processed: 10,383,426 bp Total written (filtered): 10,346,495 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3141 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 12.9% G: 79.6% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 161 596.8 0 161 4 14 149.2 0 14 5 2 37.3 0 2 8 1 0.6 0 1 9 11 0.1 0 0 11 10 39 0.0 1 1 38 11 2379 0.0 1 111 2268 12 489 0.0 1 11 478 13 18 0.0 1 1 17 14 1 0.0 1 0 1 15 4 0.0 1 0 4 20 4 0.0 1 3 1 152 6 0.0 1 3 3 153 2 0.0 1 0 2 171 1 0.0 1 1 192 1 0.0 1 1 210 1 0.0 1 1 225 4 0.0 1 4 226 1 0.0 1 1 270 1 0.0 1 1 273 1 0.0 1 1