This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.90 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 374,502 (15.3%) Reads that were too short: 328,878 (13.5%) Reads written (passing filters): 45,624 (1.9%) Total basepairs processed: 735,190,694 bp Total written (filtered): 10,552,860 bp (1.4%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 374502 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 239 596.3 0 239 7 361 596.3 0 361 8 11713 596.3 0 11713 9 13607 596.3 0 13607 10 51 596.3 0 51 11 49 596.3 0 49 12 35 596.3 0 35 13 46 596.3 0 46 14 37 596.3 0 37 15 34 596.3 0 34 16 34 596.3 0 34 17 46 596.3 0 46 18 37 596.3 0 37 19 26 596.3 0 26 20 76 596.3 0 76 21 28 596.3 0 28 22 60 596.3 0 60 23 32 596.3 0 32 24 44 596.3 0 44 25 33 596.3 0 33 26 51 596.3 0 51 27 42 596.3 0 42 28 28 596.3 0 28 29 30 596.3 0 30 30 20 596.3 0 20 31 32 596.3 0 32 32 27 596.3 0 27 33 13 596.3 0 13 34 34 596.3 0 34 35 43 596.3 0 43 36 25 596.3 0 25 37 36 596.3 0 36 38 38 596.3 0 38 39 44 596.3 0 44 40 34 596.3 0 34 41 34 596.3 0 34 42 27 596.3 0 27 43 24 596.3 0 24 44 21 596.3 0 21 45 27 596.3 0 27 46 36 596.3 0 36 47 36 596.3 0 36 48 27 596.3 0 27 49 46 596.3 0 46 50 29 596.3 0 29 51 40 596.3 0 40 52 30 596.3 0 30 53 35 596.3 0 35 54 31 596.3 0 31 55 29 596.3 0 29 56 34 596.3 0 34 57 29 596.3 0 29 58 23 596.3 0 23 59 26 596.3 0 26 60 27 596.3 0 27 61 33 596.3 0 33 62 27 596.3 0 27 63 65 596.3 0 65 64 79 596.3 0 79 65 35 596.3 0 35 66 68 596.3 0 68 67 98 596.3 0 98 68 27 596.3 0 27 69 43 596.3 0 43 70 28 596.3 0 28 71 42 596.3 0 42 72 33 596.3 0 33 73 44 596.3 0 44 74 33 596.3 0 33 75 40 596.3 0 40 76 55 596.3 0 55 77 54 596.3 0 54 78 36 596.3 0 36 79 34 596.3 0 34 80 36 596.3 0 36 81 34 596.3 0 34 82 35 596.3 0 35 83 26 596.3 0 26 84 39 596.3 0 39 85 46 596.3 0 46 86 57 596.3 0 57 87 33 596.3 0 33 88 44 596.3 0 44 89 65 596.3 0 65 90 71 596.3 0 71 91 73 596.3 0 73 92 63 596.3 0 63 93 47 596.3 0 47 94 37 596.3 0 37 95 43 596.3 0 43 96 28 596.3 0 28 97 37 596.3 0 37 98 24 596.3 0 24 99 41 596.3 0 41 100 35 596.3 0 35 101 25 596.3 0 25 102 55 596.3 0 55 103 34 596.3 0 34 104 49 596.3 0 49 105 42 596.3 0 42 106 41 596.3 0 41 107 43 596.3 0 43 108 22 596.3 0 22 109 42 596.3 0 42 110 28 596.3 0 28 111 38 596.3 0 38 112 41 596.3 0 41 113 40 596.3 0 40 114 34 596.3 0 34 115 37 596.3 0 37 116 60 596.3 0 60 117 48 596.3 0 48 118 49 596.3 0 49 119 40 596.3 0 40 120 55 596.3 0 55 121 38 596.3 0 38 122 43 596.3 0 43 123 43 596.3 0 43 124 54 596.3 0 54 125 59 596.3 0 59 126 131 596.3 0 131 127 208 596.3 0 208 128 65 596.3 0 65 129 74 596.3 0 74 130 57 596.3 0 57 131 447 596.3 0 447 132 871 596.3 0 871 133 543 596.3 0 543 134 49 596.3 0 49 135 53 596.3 0 53 136 44 596.3 0 44 137 40 596.3 0 40 138 48 596.3 0 48 139 50 596.3 0 50 140 49 596.3 0 49 141 65 596.3 0 65 142 48 596.3 0 48 143 65 596.3 0 65 144 44 596.3 0 44 145 42 596.3 0 42 146 69 596.3 0 69 147 54 596.3 0 54 148 69 596.3 0 69 149 48 596.3 0 48 150 76 596.3 0 76 151 53 596.3 0 53 152 66 596.3 0 66 153 68 596.3 0 68 154 93 596.3 0 93 155 78 596.3 0 78 156 81 596.3 0 81 157 70 596.3 0 70 158 61 596.3 0 61 159 64 596.3 0 64 160 61 596.3 0 61 161 75 596.3 0 75 162 53 596.3 0 53 163 65 596.3 0 65 164 66 596.3 0 66 165 74 596.3 0 74 166 86 596.3 0 86 167 83 596.3 0 83 168 84 596.3 0 84 169 86 596.3 0 86 170 74 596.3 0 74 171 77 596.3 0 77 172 82 596.3 0 82 173 244 596.3 0 244 174 194 596.3 0 194 175 239 596.3 0 239 176 167 596.3 0 167 177 77 596.3 0 77 178 86 596.3 0 86 179 122 596.3 0 122 180 207 596.3 0 207 181 231 596.3 0 231 182 160 596.3 0 160 183 110 596.3 0 110 184 104 596.3 0 104 185 658 596.3 0 658 186 262 596.3 0 262 187 388 596.3 0 388 188 114 596.3 0 114 189 122 596.3 0 122 190 122 596.3 0 122 191 154 596.3 0 154 192 265 596.3 0 265 193 1833 596.3 0 1833 194 1473 596.3 0 1473 195 1671 596.3 0 1671 196 142 596.3 0 142 197 91 596.3 0 91 198 93 596.3 0 93 199 213 596.3 0 213 200 310 596.3 0 310 201 319 596.3 0 319 202 200 596.3 0 200 203 89 596.3 0 89 204 90 596.3 0 90 205 113 596.3 0 113 206 143 596.3 0 143 207 165 596.3 0 165 208 124 596.3 0 124 209 98 596.3 0 98 210 114 596.3 0 114 211 185 596.3 0 185 212 133 596.3 0 133 213 321 596.3 0 321 214 189 596.3 0 189 215 191 596.3 0 191 216 153 596.3 0 153 217 147 596.3 0 147 218 176 596.3 0 176 219 308 596.3 0 308 220 255 596.3 0 255 221 303 596.3 0 303 222 386 596.3 0 386 223 630 596.3 0 630 224 1081 596.3 0 1081 225 391 596.3 0 391 226 401 596.3 0 401 227 804 596.3 0 804 228 416 596.3 0 416 229 481 596.3 0 481 230 761 596.3 0 761 231 1084 596.3 0 1084 232 6653 596.3 0 6653 233 9429 596.3 0 9429 234 8313 596.3 0 8313 235 2376 596.3 0 2376 236 1654 596.3 0 1654 237 23019 596.3 0 23019 238 44602 596.3 0 44602 239 43365 596.3 0 43365 240 8436 596.3 0 8436 241 1537 596.3 0 1537 242 1533 596.3 0 1533 243 2481 596.3 0 2481 244 1389 596.3 0 1389 245 563 596.3 0 563 246 517 596.3 0 517 247 854 596.3 0 854 248 1662 596.3 0 1662 249 758 596.3 0 758 250 1976 596.3 0 1976 251 2976 596.3 0 2976 252 2646 596.3 0 2646 253 2750 596.3 0 2750 254 4002 596.3 0 4002 255 1415 596.3 0 1415 256 1714 596.3 0 1714 257 24338 596.3 0 24338 258 52882 596.3 0 52882 259 44136 596.3 0 44136 260 13576 596.3 0 13576 261 115 596.3 0 115 262 101 596.3 0 101 263 98 596.3 0 98 264 84 596.3 0 84 265 87 596.3 0 87 266 78 596.3 0 78 267 100 596.3 0 100 268 277 596.3 0 277 269 129 596.3 0 129 270 222 596.3 0 222 271 154 596.3 0 154 272 1190 596.3 0 1190 273 78 596.3 0 78 274 66 596.3 0 66 275 77 596.3 0 77 276 97 596.3 0 97 277 55 596.3 0 55 278 70 596.3 0 70 279 97 596.3 0 97 280 107 596.3 0 107 281 95 596.3 0 95 282 107 596.3 0 107 283 90 596.3 0 90 284 72 596.3 0 72 285 95 596.3 0 95 286 118 596.3 0 118 287 126 596.3 0 126 288 112 596.3 0 112 289 132 596.3 0 132 290 225 596.3 0 225 291 625 596.3 0 625 292 672 596.3 0 672 293 620 596.3 0 620 294 291 596.3 0 291 295 134 596.3 0 134 296 132 596.3 0 132 297 108 596.3 0 108 298 74 596.3 0 74 299 69 596.3 0 69 300 95 596.3 0 95 301 120 596.3 0 120 Finished in 1.99 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 45,624 Reads with adapters: 11,421 (25.0%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 11,391 (25.0%) Total basepairs processed: 10,552,860 bp Total written (filtered): 3,139,709 bp (29.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11421 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 3 16 81 0.0 3 0 79 1 1 17 11222 0.0 3 11007 208 6 1 18 36 0.0 3 1 30 3 2 19 5 0.0 3 0 0 5 20 13 0.0 3 1 0 0 12 24 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 1 30 1 0.0 3 1 31 4 0.0 3 0 0 0 4 33 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 6 0.0 3 0 0 0 6 48 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 60 7 0.0 3 0 0 0 7 63 3 0.0 3 0 0 1 2 64 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 195 3 0.0 3 0 0 0 3 198 1 0.0 3 0 0 0 1 249 2 0.0 3 0 1 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 11,391 Reads with adapters: 73 (0.6%) Reads written (passing filters): 11,391 (100.0%) Total basepairs processed: 3,139,709 bp Total written (filtered): 3,139,470 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 73 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.0% C: 16.4% G: 8.2% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 53 178.0 0 53 4 20 44.5 0 20