This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.77 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 173,641 (7.1%) Reads that were too short: 14,511 (0.6%) Reads written (passing filters): 159,130 (6.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 44,330,108 bp (6.0%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 173641 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1774 596.3 0 1774 7 61951 596.3 0 61951 8 1554 596.3 0 1554 9 73596 596.3 0 73596 10 65 596.3 0 65 11 19 596.3 0 19 12 9 596.3 0 9 13 16 596.3 0 16 14 9 596.3 0 9 15 8 596.3 0 8 16 16 596.3 0 16 17 36 596.3 0 36 18 54 596.3 0 54 19 4 596.3 0 4 20 13 596.3 0 13 21 10 596.3 0 10 22 16 596.3 0 16 23 30 596.3 0 30 24 21 596.3 0 21 25 26 596.3 0 26 26 159 596.3 0 159 27 202 596.3 0 202 28 328 596.3 0 328 29 101 596.3 0 101 30 502 596.3 0 502 31 106 596.3 0 106 32 7 596.3 0 7 33 8 596.3 0 8 34 4 596.3 0 4 35 16 596.3 0 16 36 13 596.3 0 13 37 7 596.3 0 7 38 8 596.3 0 8 39 6 596.3 0 6 40 10 596.3 0 10 41 5 596.3 0 5 42 18 596.3 0 18 43 10 596.3 0 10 44 16 596.3 0 16 45 12 596.3 0 12 46 12 596.3 0 12 47 9 596.3 0 9 48 3 596.3 0 3 49 10 596.3 0 10 50 11 596.3 0 11 51 15 596.3 0 15 52 20 596.3 0 20 53 11 596.3 0 11 54 17 596.3 0 17 55 6 596.3 0 6 56 9 596.3 0 9 57 17 596.3 0 17 58 13 596.3 0 13 59 20 596.3 0 20 60 17 596.3 0 17 61 17 596.3 0 17 62 21 596.3 0 21 63 31 596.3 0 31 64 30 596.3 0 30 65 28 596.3 0 28 66 17 596.3 0 17 67 44 596.3 0 44 68 73 596.3 0 73 69 752 596.3 0 752 70 424 596.3 0 424 71 1983 596.3 0 1983 72 39 596.3 0 39 73 19 596.3 0 19 74 24 596.3 0 24 75 50 596.3 0 50 76 27 596.3 0 27 77 126 596.3 0 126 78 54 596.3 0 54 79 31 596.3 0 31 80 20 596.3 0 20 81 17 596.3 0 17 82 38 596.3 0 38 83 16 596.3 0 16 84 21 596.3 0 21 85 21 596.3 0 21 86 27 596.3 0 27 87 36 596.3 0 36 88 25 596.3 0 25 89 18 596.3 0 18 90 29 596.3 0 29 91 24 596.3 0 24 92 20 596.3 0 20 93 37 596.3 0 37 94 21 596.3 0 21 95 13 596.3 0 13 96 38 596.3 0 38 97 65 596.3 0 65 98 35 596.3 0 35 99 29 596.3 0 29 100 25 596.3 0 25 101 30 596.3 0 30 102 19 596.3 0 19 103 7 596.3 0 7 104 13 596.3 0 13 105 17 596.3 0 17 106 16 596.3 0 16 107 23 596.3 0 23 108 21 596.3 0 21 109 28 596.3 0 28 110 38 596.3 0 38 111 35 596.3 0 35 112 29 596.3 0 29 113 32 596.3 0 32 114 111 596.3 0 111 115 232 596.3 0 232 116 84 596.3 0 84 117 19 596.3 0 19 118 16 596.3 0 16 119 12 596.3 0 12 120 13 596.3 0 13 121 38 596.3 0 38 122 23 596.3 0 23 123 28 596.3 0 28 124 31 596.3 0 31 125 13 596.3 0 13 126 23 596.3 0 23 127 46 596.3 0 46 128 82 596.3 0 82 129 47 596.3 0 47 130 57 596.3 0 57 131 51 596.3 0 51 132 52 596.3 0 52 133 167 596.3 0 167 134 78 596.3 0 78 135 98 596.3 0 98 136 73 596.3 0 73 137 88 596.3 0 88 138 384 596.3 0 384 139 897 596.3 0 897 140 279 596.3 0 279 141 246 596.3 0 246 142 374 596.3 0 374 143 322 596.3 0 322 144 337 596.3 0 337 145 599 596.3 0 599 146 91 596.3 0 91 147 91 596.3 0 91 148 978 596.3 0 978 149 2031 596.3 0 2031 150 1361 596.3 0 1361 151 1567 596.3 0 1567 152 83 596.3 0 83 153 76 596.3 0 76 154 31 596.3 0 31 155 43 596.3 0 43 156 22 596.3 0 22 157 38 596.3 0 38 158 25 596.3 0 25 159 40 596.3 0 40 160 26 596.3 0 26 161 23 596.3 0 23 162 26 596.3 0 26 163 20 596.3 0 20 164 21 596.3 0 21 165 11 596.3 0 11 166 24 596.3 0 24 167 16 596.3 0 16 168 20 596.3 0 20 169 28 596.3 0 28 170 27 596.3 0 27 171 16 596.3 0 16 172 20 596.3 0 20 173 23 596.3 0 23 174 23 596.3 0 23 175 35 596.3 0 35 176 39 596.3 0 39 177 24 596.3 0 24 178 18 596.3 0 18 179 41 596.3 0 41 180 26 596.3 0 26 181 30 596.3 0 30 182 28 596.3 0 28 183 30 596.3 0 30 184 52 596.3 0 52 185 95 596.3 0 95 186 78 596.3 0 78 187 32 596.3 0 32 188 49 596.3 0 49 189 143 596.3 0 143 190 195 596.3 0 195 191 316 596.3 0 316 192 138 596.3 0 138 193 75 596.3 0 75 194 49 596.3 0 49 195 41 596.3 0 41 196 30 596.3 0 30 197 43 596.3 0 43 198 58 596.3 0 58 199 44 596.3 0 44 200 37 596.3 0 37 201 35 596.3 0 35 202 36 596.3 0 36 203 43 596.3 0 43 204 57 596.3 0 57 205 53 596.3 0 53 206 49 596.3 0 49 207 41 596.3 0 41 208 28 596.3 0 28 209 146 596.3 0 146 210 286 596.3 0 286 211 129 596.3 0 129 212 66 596.3 0 66 213 84 596.3 0 84 214 160 596.3 0 160 215 145 596.3 0 145 216 31 596.3 0 31 217 40 596.3 0 40 218 30 596.3 0 30 219 51 596.3 0 51 220 31 596.3 0 31 221 37 596.3 0 37 222 39 596.3 0 39 223 64 596.3 0 64 224 51 596.3 0 51 225 51 596.3 0 51 226 30 596.3 0 30 227 41 596.3 0 41 228 50 596.3 0 50 229 97 596.3 0 97 230 79 596.3 0 79 231 42 596.3 0 42 232 58 596.3 0 58 233 165 596.3 0 165 234 363 596.3 0 363 235 432 596.3 0 432 236 182 596.3 0 182 237 79 596.3 0 79 238 58 596.3 0 58 239 77 596.3 0 77 240 70 596.3 0 70 241 70 596.3 0 70 242 102 596.3 0 102 243 91 596.3 0 91 244 143 596.3 0 143 245 105 596.3 0 105 246 125 596.3 0 125 247 93 596.3 0 93 248 135 596.3 0 135 249 212 596.3 0 212 250 139 596.3 0 139 251 288 596.3 0 288 252 116 596.3 0 116 253 652 596.3 0 652 254 1462 596.3 0 1462 255 1250 596.3 0 1250 256 186 596.3 0 186 257 80 596.3 0 80 258 72 596.3 0 72 259 52 596.3 0 52 260 68 596.3 0 68 261 67 596.3 0 67 262 97 596.3 0 97 263 52 596.3 0 52 264 68 596.3 0 68 265 58 596.3 0 58 266 106 596.3 0 106 267 93 596.3 0 93 268 156 596.3 0 156 269 176 596.3 0 176 270 109 596.3 0 109 271 84 596.3 0 84 272 86 596.3 0 86 273 259 596.3 0 259 274 397 596.3 0 397 275 271 596.3 0 271 276 112 596.3 0 112 277 50 596.3 0 50 278 135 596.3 0 135 279 103 596.3 0 103 280 107 596.3 0 107 281 117 596.3 0 117 282 86 596.3 0 86 283 109 596.3 0 109 284 75 596.3 0 75 285 96 596.3 0 96 286 112 596.3 0 112 287 124 596.3 0 124 288 95 596.3 0 95 289 110 596.3 0 110 290 76 596.3 0 76 291 72 596.3 0 72 292 192 596.3 0 192 293 190 596.3 0 190 294 202 596.3 0 202 295 143 596.3 0 143 296 123 596.3 0 123 297 185 596.3 0 185 298 122 596.3 0 122 299 149 596.3 0 149 300 263 596.3 0 263 301 272 596.3 0 272 Finished in 8.26 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 159,130 Reads with adapters: 63,619 (40.0%) Reads that were too short: 90 (0.1%) Reads written (passing filters): 63,529 (39.9%) Total basepairs processed: 44,330,108 bp Total written (filtered): 17,323,841 bp (39.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63619 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 6 0.0 3 0 0 0 4 2 18 28 0.0 3 0 0 17 5 6 19 817 0.0 3 0 705 71 29 12 20 61618 0.0 4 57594 3309 525 145 45 21 141 0.0 4 23 105 6 3 4 22 21 0.0 4 3 1 14 1 2 23 120 0.0 4 7 0 0 87 26 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 1 84 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 98 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 0 1 108 12 0.0 4 0 0 0 0 12 112 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 10 0.0 4 0 0 0 0 10 127 715 0.0 4 0 0 0 0 715 128 11 0.0 4 0 0 0 0 11 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 146 1 0.0 4 1 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 168 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 203 2 0.0 4 0 0 0 0 2 212 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 1 221 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 227 3 0.0 4 0 0 0 0 3 228 32 0.0 4 0 0 0 0 32 229 9 0.0 4 0 0 0 0 9 230 3 0.0 4 0 0 0 0 3 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 0 3 266 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.24 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 63,529 Reads with adapters: 3,330 (5.2%) Reads written (passing filters): 63,529 (100.0%) Total basepairs processed: 17,323,841 bp Total written (filtered): 17,267,546 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3330 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.8% C: 14.5% G: 72.7% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 353 992.6 0 353 4 42 248.2 0 42 5 2 62.0 0 2 9 20 0.2 0 0 20 10 250 0.1 1 14 236 11 1550 0.0 1 73 1477 12 966 0.0 1 20 946 13 33 0.0 1 2 31 14 4 0.0 1 0 4 15 4 0.0 1 0 4 152 6 0.0 1 5 1 191 2 0.0 1 0 2 210 19 0.0 1 16 3 222 1 0.0 1 0 1 225 74 0.0 1 69 5 226 1 0.0 1 0 1 269 1 0.0 1 0 1 270 2 0.0 1 2