This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.51 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 85,431 (3.5%) Reads that were too short: 30,206 (1.2%) Reads written (passing filters): 55,225 (2.3%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,868,338 bp (2.0%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 85431 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 690 596.3 0 690 7 589 596.3 0 589 8 22986 596.3 0 22986 9 17971 596.3 0 17971 10 18 596.3 0 18 11 2 596.3 0 2 12 3 596.3 0 3 13 2 596.3 0 2 14 4 596.3 0 4 15 1 596.3 0 1 16 2 596.3 0 2 17 5 596.3 0 5 18 4 596.3 0 4 19 7 596.3 0 7 20 2 596.3 0 2 21 1 596.3 0 1 22 2 596.3 0 2 23 5 596.3 0 5 24 5 596.3 0 5 25 6 596.3 0 6 26 10 596.3 0 10 27 53 596.3 0 53 28 19 596.3 0 19 29 10 596.3 0 10 30 4 596.3 0 4 31 4 596.3 0 4 32 7 596.3 0 7 33 23 596.3 0 23 34 5 596.3 0 5 35 4 596.3 0 4 36 1 596.3 0 1 37 2 596.3 0 2 38 3 596.3 0 3 39 7 596.3 0 7 40 15 596.3 0 15 41 4 596.3 0 4 42 5 596.3 0 5 43 13 596.3 0 13 44 10 596.3 0 10 45 7 596.3 0 7 46 10 596.3 0 10 47 4 596.3 0 4 48 6 596.3 0 6 49 12 596.3 0 12 50 12 596.3 0 12 51 10 596.3 0 10 52 21 596.3 0 21 53 17 596.3 0 17 54 19 596.3 0 19 55 16 596.3 0 16 56 16 596.3 0 16 57 27 596.3 0 27 58 51 596.3 0 51 59 196 596.3 0 196 60 1425 596.3 0 1425 61 792 596.3 0 792 62 1663 596.3 0 1663 63 19 596.3 0 19 64 15 596.3 0 15 65 20 596.3 0 20 66 98 596.3 0 98 67 31 596.3 0 31 68 58 596.3 0 58 69 6 596.3 0 6 70 13 596.3 0 13 71 12 596.3 0 12 72 19 596.3 0 19 73 8 596.3 0 8 74 11 596.3 0 11 75 15 596.3 0 15 76 27 596.3 0 27 77 281 596.3 0 281 78 97 596.3 0 97 79 331 596.3 0 331 80 17 596.3 0 17 81 36 596.3 0 36 82 15 596.3 0 15 83 26 596.3 0 26 84 62 596.3 0 62 85 18 596.3 0 18 86 32 596.3 0 32 87 12 596.3 0 12 88 12 596.3 0 12 89 5 596.3 0 5 90 30 596.3 0 30 91 17 596.3 0 17 92 8 596.3 0 8 93 20 596.3 0 20 94 47 596.3 0 47 95 36 596.3 0 36 96 6 596.3 0 6 97 20 596.3 0 20 98 17 596.3 0 17 99 23 596.3 0 23 100 15 596.3 0 15 101 10 596.3 0 10 102 18 596.3 0 18 103 18 596.3 0 18 104 34 596.3 0 34 105 15 596.3 0 15 106 16 596.3 0 16 107 18 596.3 0 18 108 18 596.3 0 18 109 18 596.3 0 18 110 21 596.3 0 21 111 83 596.3 0 83 112 191 596.3 0 191 113 254 596.3 0 254 114 790 596.3 0 790 115 812 596.3 0 812 116 533 596.3 0 533 117 124 596.3 0 124 118 16 596.3 0 16 119 12 596.3 0 12 120 9 596.3 0 9 121 14 596.3 0 14 122 13 596.3 0 13 123 16 596.3 0 16 124 21 596.3 0 21 125 11 596.3 0 11 126 13 596.3 0 13 127 14 596.3 0 14 128 12 596.3 0 12 129 19 596.3 0 19 130 25 596.3 0 25 131 49 596.3 0 49 132 35 596.3 0 35 133 34 596.3 0 34 134 17 596.3 0 17 135 26 596.3 0 26 136 21 596.3 0 21 137 50 596.3 0 50 138 28 596.3 0 28 139 35 596.3 0 35 140 24 596.3 0 24 141 17 596.3 0 17 142 21 596.3 0 21 143 44 596.3 0 44 144 44 596.3 0 44 145 41 596.3 0 41 146 36 596.3 0 36 147 33 596.3 0 33 148 20 596.3 0 20 149 55 596.3 0 55 150 30 596.3 0 30 151 15 596.3 0 15 152 21 596.3 0 21 153 18 596.3 0 18 154 21 596.3 0 21 155 17 596.3 0 17 156 31 596.3 0 31 157 20 596.3 0 20 158 11 596.3 0 11 159 21 596.3 0 21 160 18 596.3 0 18 161 22 596.3 0 22 162 21 596.3 0 21 163 47 596.3 0 47 164 119 596.3 0 119 165 151 596.3 0 151 166 99 596.3 0 99 167 82 596.3 0 82 168 22 596.3 0 22 169 8 596.3 0 8 170 17 596.3 0 17 171 32 596.3 0 32 172 14 596.3 0 14 173 15 596.3 0 15 174 17 596.3 0 17 175 10 596.3 0 10 176 26 596.3 0 26 177 17 596.3 0 17 178 33 596.3 0 33 179 134 596.3 0 134 180 83 596.3 0 83 181 223 596.3 0 223 182 32 596.3 0 32 183 27 596.3 0 27 184 76 596.3 0 76 185 70 596.3 0 70 186 73 596.3 0 73 187 51 596.3 0 51 188 26 596.3 0 26 189 66 596.3 0 66 190 147 596.3 0 147 191 377 596.3 0 377 192 247 596.3 0 247 193 101 596.3 0 101 194 18 596.3 0 18 195 29 596.3 0 29 196 43 596.3 0 43 197 74 596.3 0 74 198 68 596.3 0 68 199 53 596.3 0 53 200 46 596.3 0 46 201 45 596.3 0 45 202 85 596.3 0 85 203 259 596.3 0 259 204 72 596.3 0 72 205 265 596.3 0 265 206 35 596.3 0 35 207 85 596.3 0 85 208 142 596.3 0 142 209 139 596.3 0 139 210 148 596.3 0 148 211 65 596.3 0 65 212 109 596.3 0 109 213 41 596.3 0 41 214 57 596.3 0 57 215 41 596.3 0 41 216 116 596.3 0 116 217 108 596.3 0 108 218 91 596.3 0 91 219 70 596.3 0 70 220 39 596.3 0 39 221 37 596.3 0 37 222 37 596.3 0 37 223 33 596.3 0 33 224 87 596.3 0 87 225 106 596.3 0 106 226 137 596.3 0 137 227 66 596.3 0 66 228 73 596.3 0 73 229 39 596.3 0 39 230 53 596.3 0 53 231 104 596.3 0 104 232 163 596.3 0 163 233 114 596.3 0 114 234 119 596.3 0 119 235 188 596.3 0 188 236 461 596.3 0 461 237 497 596.3 0 497 238 543 596.3 0 543 239 369 596.3 0 369 240 115 596.3 0 115 241 121 596.3 0 121 242 151 596.3 0 151 243 176 596.3 0 176 244 334 596.3 0 334 245 4268 596.3 0 4268 246 5031 596.3 0 5031 247 6491 596.3 0 6491 248 80 596.3 0 80 249 44 596.3 0 44 250 61 596.3 0 61 251 53 596.3 0 53 252 67 596.3 0 67 253 95 596.3 0 95 254 119 596.3 0 119 255 85 596.3 0 85 256 76 596.3 0 76 257 48 596.3 0 48 258 59 596.3 0 59 259 93 596.3 0 93 260 346 596.3 0 346 261 192 596.3 0 192 262 338 596.3 0 338 263 123 596.3 0 123 264 169 596.3 0 169 265 815 596.3 0 815 266 949 596.3 0 949 267 476 596.3 0 476 268 61 596.3 0 61 269 48 596.3 0 48 270 162 596.3 0 162 271 291 596.3 0 291 272 665 596.3 0 665 273 198 596.3 0 198 274 34 596.3 0 34 275 35 596.3 0 35 276 29 596.3 0 29 277 109 596.3 0 109 278 122 596.3 0 122 279 198 596.3 0 198 280 167 596.3 0 167 281 94 596.3 0 94 282 45 596.3 0 45 283 67 596.3 0 67 284 109 596.3 0 109 285 85 596.3 0 85 286 91 596.3 0 91 287 79 596.3 0 79 288 67 596.3 0 67 289 99 596.3 0 99 290 86 596.3 0 86 291 38 596.3 0 38 292 47 596.3 0 47 293 77 596.3 0 77 294 101 596.3 0 101 295 97 596.3 0 97 296 84 596.3 0 84 297 76 596.3 0 76 298 225 596.3 0 225 299 135 596.3 0 135 300 122 596.3 0 122 301 95 596.3 0 95 Finished in 2.83 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 55,225 Reads with adapters: 20,401 (36.9%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 20,374 (36.9%) Total basepairs processed: 14,868,338 bp Total written (filtered): 5,416,496 bp (36.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20401 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 13 0.0 3 0 0 8 4 1 19 338 0.0 3 0 304 20 11 3 20 18344 0.0 4 17376 791 133 29 15 21 67 0.0 4 14 42 5 4 2 22 354 0.0 4 330 17 5 0 2 23 64 0.0 4 0 1 0 46 17 33 1 0.0 4 1 54 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 18 0.0 4 0 0 0 0 18 127 1153 0.0 4 0 0 0 0 1153 128 7 0.0 4 0 0 0 0 7 130 5 0.0 4 0 0 0 0 5 151 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 1 209 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.72 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 20,374 Reads with adapters: 4,360 (21.4%) Reads written (passing filters): 20,374 (100.0%) Total basepairs processed: 5,416,496 bp Total written (filtered): 5,368,982 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4360 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 9.5% G: 85.6% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 318.3 0 13 4 1 79.6 0 1 9 501 0.1 0 47 454 10 3249 0.0 1 245 3004 11 541 0.0 1 24 517 12 14 0.0 1 1 13 13 17 0.0 1 0 17 14 1 0.0 1 0 1 18 2 0.0 1 2 23 1 0.0 1 1 121 1 0.0 1 0 1 152 1 0.0 1 1 153 2 0.0 1 2 208 5 0.0 1 3 2 209 1 0.0 1 1 223 9 0.0 1 7 2 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.