This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.21 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 77,008 (3.2%) Reads that were too short: 25,987 (1.1%) Reads written (passing filters): 51,021 (2.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,147,822 bp (1.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 77008 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 726 596.3 0 726 7 491 596.3 0 491 8 20378 596.3 0 20378 9 22031 596.3 0 22031 10 16 596.3 0 16 11 2 596.3 0 2 12 3 596.3 0 3 13 1 596.3 0 1 14 3 596.3 0 3 16 2 596.3 0 2 17 2 596.3 0 2 18 5 596.3 0 5 19 2 596.3 0 2 20 2 596.3 0 2 21 2 596.3 0 2 22 1 596.3 0 1 23 3 596.3 0 3 24 2 596.3 0 2 25 5 596.3 0 5 26 6 596.3 0 6 27 2 596.3 0 2 28 2 596.3 0 2 29 2 596.3 0 2 30 3 596.3 0 3 31 2 596.3 0 2 32 2 596.3 0 2 33 10 596.3 0 10 34 2 596.3 0 2 35 1 596.3 0 1 36 3 596.3 0 3 37 3 596.3 0 3 38 2 596.3 0 2 39 7 596.3 0 7 40 9 596.3 0 9 41 9 596.3 0 9 42 8 596.3 0 8 43 16 596.3 0 16 44 4 596.3 0 4 45 3 596.3 0 3 46 4 596.3 0 4 47 4 596.3 0 4 48 9 596.3 0 9 49 13 596.3 0 13 50 6 596.3 0 6 51 6 596.3 0 6 52 3 596.3 0 3 53 5 596.3 0 5 54 6 596.3 0 6 55 11 596.3 0 11 56 13 596.3 0 13 57 27 596.3 0 27 58 51 596.3 0 51 59 108 596.3 0 108 60 717 596.3 0 717 61 342 596.3 0 342 62 985 596.3 0 985 63 12 596.3 0 12 64 7 596.3 0 7 65 5 596.3 0 5 66 58 596.3 0 58 67 45 596.3 0 45 68 41 596.3 0 41 69 4 596.3 0 4 70 6 596.3 0 6 71 3 596.3 0 3 72 10 596.3 0 10 73 8 596.3 0 8 74 5 596.3 0 5 75 8 596.3 0 8 76 10 596.3 0 10 77 131 596.3 0 131 78 62 596.3 0 62 79 253 596.3 0 253 80 12 596.3 0 12 81 26 596.3 0 26 82 12 596.3 0 12 83 19 596.3 0 19 84 12 596.3 0 12 85 12 596.3 0 12 86 35 596.3 0 35 87 6 596.3 0 6 88 8 596.3 0 8 89 9 596.3 0 9 90 32 596.3 0 32 91 7 596.3 0 7 92 12 596.3 0 12 93 8 596.3 0 8 94 14 596.3 0 14 95 17 596.3 0 17 96 6 596.3 0 6 97 18 596.3 0 18 98 15 596.3 0 15 99 28 596.3 0 28 100 17 596.3 0 17 101 10 596.3 0 10 102 7 596.3 0 7 103 12 596.3 0 12 104 24 596.3 0 24 105 7 596.3 0 7 106 6 596.3 0 6 107 13 596.3 0 13 108 11 596.3 0 11 109 16 596.3 0 16 110 10 596.3 0 10 111 43 596.3 0 43 112 90 596.3 0 90 113 116 596.3 0 116 114 219 596.3 0 219 115 341 596.3 0 341 116 187 596.3 0 187 117 85 596.3 0 85 118 8 596.3 0 8 119 6 596.3 0 6 120 4 596.3 0 4 121 7 596.3 0 7 122 15 596.3 0 15 123 17 596.3 0 17 124 10 596.3 0 10 125 14 596.3 0 14 126 15 596.3 0 15 127 8 596.3 0 8 128 8 596.3 0 8 129 8 596.3 0 8 130 13 596.3 0 13 131 14 596.3 0 14 132 12 596.3 0 12 133 26 596.3 0 26 134 12 596.3 0 12 135 11 596.3 0 11 136 14 596.3 0 14 137 36 596.3 0 36 138 25 596.3 0 25 139 17 596.3 0 17 140 15 596.3 0 15 141 5 596.3 0 5 142 11 596.3 0 11 143 22 596.3 0 22 144 22 596.3 0 22 145 21 596.3 0 21 146 12 596.3 0 12 147 6 596.3 0 6 148 10 596.3 0 10 149 40 596.3 0 40 150 21 596.3 0 21 151 15 596.3 0 15 152 21 596.3 0 21 153 9 596.3 0 9 154 10 596.3 0 10 155 11 596.3 0 11 156 15 596.3 0 15 157 6 596.3 0 6 158 11 596.3 0 11 159 16 596.3 0 16 160 14 596.3 0 14 161 15 596.3 0 15 162 11 596.3 0 11 163 13 596.3 0 13 164 51 596.3 0 51 165 47 596.3 0 47 166 47 596.3 0 47 167 38 596.3 0 38 168 17 596.3 0 17 169 7 596.3 0 7 170 16 596.3 0 16 171 14 596.3 0 14 172 14 596.3 0 14 173 7 596.3 0 7 174 12 596.3 0 12 175 12 596.3 0 12 176 10 596.3 0 10 177 9 596.3 0 9 178 28 596.3 0 28 179 137 596.3 0 137 180 90 596.3 0 90 181 228 596.3 0 228 182 14 596.3 0 14 183 15 596.3 0 15 184 40 596.3 0 40 185 24 596.3 0 24 186 25 596.3 0 25 187 36 596.3 0 36 188 21 596.3 0 21 189 52 596.3 0 52 190 157 596.3 0 157 191 500 596.3 0 500 192 138 596.3 0 138 193 116 596.3 0 116 194 19 596.3 0 19 195 10 596.3 0 10 196 29 596.3 0 29 197 23 596.3 0 23 198 23 596.3 0 23 199 21 596.3 0 21 200 23 596.3 0 23 201 26 596.3 0 26 202 94 596.3 0 94 203 250 596.3 0 250 204 57 596.3 0 57 205 332 596.3 0 332 206 14 596.3 0 14 207 38 596.3 0 38 208 79 596.3 0 79 209 55 596.3 0 55 210 79 596.3 0 79 211 30 596.3 0 30 212 96 596.3 0 96 213 23 596.3 0 23 214 41 596.3 0 41 215 21 596.3 0 21 216 34 596.3 0 34 217 47 596.3 0 47 218 25 596.3 0 25 219 40 596.3 0 40 220 12 596.3 0 12 221 29 596.3 0 29 222 23 596.3 0 23 223 20 596.3 0 20 224 45 596.3 0 45 225 37 596.3 0 37 226 36 596.3 0 36 227 32 596.3 0 32 228 23 596.3 0 23 229 32 596.3 0 32 230 38 596.3 0 38 231 63 596.3 0 63 232 137 596.3 0 137 233 69 596.3 0 69 234 71 596.3 0 71 235 141 596.3 0 141 236 254 596.3 0 254 237 305 596.3 0 305 238 241 596.3 0 241 239 193 596.3 0 193 240 86 596.3 0 86 241 92 596.3 0 92 242 108 596.3 0 108 243 142 596.3 0 142 244 229 596.3 0 229 245 4411 596.3 0 4411 246 5428 596.3 0 5428 247 6749 596.3 0 6749 248 58 596.3 0 58 249 24 596.3 0 24 250 33 596.3 0 33 251 38 596.3 0 38 252 44 596.3 0 44 253 52 596.3 0 52 254 59 596.3 0 59 255 38 596.3 0 38 256 43 596.3 0 43 257 27 596.3 0 27 258 43 596.3 0 43 259 46 596.3 0 46 260 304 596.3 0 304 261 161 596.3 0 161 262 348 596.3 0 348 263 87 596.3 0 87 264 82 596.3 0 82 265 740 596.3 0 740 266 791 596.3 0 791 267 254 596.3 0 254 268 30 596.3 0 30 269 25 596.3 0 25 270 81 596.3 0 81 271 117 596.3 0 117 272 295 596.3 0 295 273 171 596.3 0 171 274 15 596.3 0 15 275 30 596.3 0 30 276 18 596.3 0 18 277 52 596.3 0 52 278 36 596.3 0 36 279 51 596.3 0 51 280 64 596.3 0 64 281 37 596.3 0 37 282 21 596.3 0 21 283 26 596.3 0 26 284 51 596.3 0 51 285 49 596.3 0 49 286 51 596.3 0 51 287 51 596.3 0 51 288 42 596.3 0 42 289 44 596.3 0 44 290 76 596.3 0 76 291 27 596.3 0 27 292 43 596.3 0 43 293 47 596.3 0 47 294 104 596.3 0 104 295 68 596.3 0 68 296 56 596.3 0 56 297 55 596.3 0 55 298 252 596.3 0 252 299 77 596.3 0 77 300 102 596.3 0 102 301 50 596.3 0 50 Finished in 2.44 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 51,021 Reads with adapters: 20,194 (39.6%) Reads that were too short: 32 (0.1%) Reads written (passing filters): 20,162 (39.5%) Total basepairs processed: 14,147,822 bp Total written (filtered): 5,562,198 bp (39.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20194 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 168 0.0 3 0 164 4 17 19893 0.0 3 19457 422 12 2 18 52 0.0 3 6 45 1 19 11 0.0 3 1 0 10 20 11 0.0 3 0 0 0 11 21 1 0.0 3 1 22 2 0.0 3 0 0 0 2 26 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 36 1 0.0 3 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 4 0.0 3 0 0 0 4 80 1 0.0 3 0 0 0 1 81 2 0.0 3 1 0 0 1 84 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 120 2 0.0 3 1 0 0 1 124 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 148 7 0.0 3 0 0 0 7 164 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.70 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 20,162 Reads with adapters: 93 (0.5%) Reads written (passing filters): 20,162 (100.0%) Total basepairs processed: 5,562,198 bp Total written (filtered): 5,561,894 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 93 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.6% C: 5.4% G: 11.8% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 79 315.0 0 79 4 11 78.8 0 11 5 1 19.7 0 1 6 1 4.9 0 1 12 1 0.0 1 0 1