This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.91 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 263,756 (10.7%) Reads that were too short: 88,702 (3.6%) Reads written (passing filters): 175,054 (7.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 48,396,279 bp (6.5%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 263756 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2263 601.0 0 2263 7 81013 601.0 0 81013 8 66017 601.0 0 66017 9 121 601.0 0 121 10 77 601.0 0 77 11 86 601.0 0 86 12 49 601.0 0 49 13 73 601.0 0 73 14 76 601.0 0 76 15 131 601.0 0 131 16 124 601.0 0 124 17 98 601.0 0 98 18 47 601.0 0 47 19 63 601.0 0 63 20 75 601.0 0 75 21 58 601.0 0 58 22 60 601.0 0 60 23 52 601.0 0 52 24 68 601.0 0 68 25 80 601.0 0 80 26 59 601.0 0 59 27 45 601.0 0 45 28 45 601.0 0 45 29 57 601.0 0 57 30 59 601.0 0 59 31 75 601.0 0 75 32 66 601.0 0 66 33 52 601.0 0 52 34 91 601.0 0 91 35 88 601.0 0 88 36 71 601.0 0 71 37 67 601.0 0 67 38 70 601.0 0 70 39 51 601.0 0 51 40 71 601.0 0 71 41 83 601.0 0 83 42 69 601.0 0 69 43 133 601.0 0 133 44 72 601.0 0 72 45 73 601.0 0 73 46 76 601.0 0 76 47 74 601.0 0 74 48 76 601.0 0 76 49 64 601.0 0 64 50 60 601.0 0 60 51 67 601.0 0 67 52 155 601.0 0 155 53 139 601.0 0 139 54 127 601.0 0 127 55 95 601.0 0 95 56 90 601.0 0 90 57 59 601.0 0 59 58 77 601.0 0 77 59 127 601.0 0 127 60 97 601.0 0 97 61 287 601.0 0 287 62 82 601.0 0 82 63 93 601.0 0 93 64 100 601.0 0 100 65 86 601.0 0 86 66 81 601.0 0 81 67 81 601.0 0 81 68 98 601.0 0 98 69 82 601.0 0 82 70 85 601.0 0 85 71 108 601.0 0 108 72 160 601.0 0 160 73 243 601.0 0 243 74 302 601.0 0 302 75 115 601.0 0 115 76 70 601.0 0 70 77 75 601.0 0 75 78 89 601.0 0 89 79 91 601.0 0 91 80 104 601.0 0 104 81 137 601.0 0 137 82 95 601.0 0 95 83 103 601.0 0 103 84 76 601.0 0 76 85 98 601.0 0 98 86 101 601.0 0 101 87 81 601.0 0 81 88 77 601.0 0 77 89 68 601.0 0 68 90 79 601.0 0 79 91 75 601.0 0 75 92 83 601.0 0 83 93 83 601.0 0 83 94 85 601.0 0 85 95 97 601.0 0 97 96 116 601.0 0 116 97 77 601.0 0 77 98 86 601.0 0 86 99 81 601.0 0 81 100 84 601.0 0 84 101 88 601.0 0 88 102 149 601.0 0 149 103 146 601.0 0 146 104 147 601.0 0 147 105 100 601.0 0 100 106 118 601.0 0 118 107 144 601.0 0 144 108 97 601.0 0 97 109 74 601.0 0 74 110 93 601.0 0 93 111 102 601.0 0 102 112 96 601.0 0 96 113 82 601.0 0 82 114 105 601.0 0 105 115 69 601.0 0 69 116 76 601.0 0 76 117 68 601.0 0 68 118 86 601.0 0 86 119 72 601.0 0 72 120 97 601.0 0 97 121 110 601.0 0 110 122 142 601.0 0 142 123 124 601.0 0 124 124 455 601.0 0 455 125 406 601.0 0 406 126 310 601.0 0 310 127 98 601.0 0 98 128 98 601.0 0 98 129 76 601.0 0 76 130 128 601.0 0 128 131 93 601.0 0 93 132 129 601.0 0 129 133 177 601.0 0 177 134 243 601.0 0 243 135 286 601.0 0 286 136 100 601.0 0 100 137 142 601.0 0 142 138 116 601.0 0 116 139 230 601.0 0 230 140 105 601.0 0 105 141 144 601.0 0 144 142 102 601.0 0 102 143 115 601.0 0 115 144 103 601.0 0 103 145 150 601.0 0 150 146 229 601.0 0 229 147 283 601.0 0 283 148 226 601.0 0 226 149 410 601.0 0 410 150 175 601.0 0 175 151 152 601.0 0 152 152 145 601.0 0 145 153 130 601.0 0 130 154 139 601.0 0 139 155 130 601.0 0 130 156 139 601.0 0 139 157 163 601.0 0 163 158 234 601.0 0 234 159 207 601.0 0 207 160 288 601.0 0 288 161 182 601.0 0 182 162 263 601.0 0 263 163 140 601.0 0 140 164 130 601.0 0 130 165 147 601.0 0 147 166 189 601.0 0 189 167 210 601.0 0 210 168 193 601.0 0 193 169 387 601.0 0 387 170 337 601.0 0 337 171 495 601.0 0 495 172 317 601.0 0 317 173 250 601.0 0 250 174 146 601.0 0 146 175 124 601.0 0 124 176 113 601.0 0 113 177 99 601.0 0 99 178 109 601.0 0 109 179 147 601.0 0 147 180 218 601.0 0 218 181 240 601.0 0 240 182 193 601.0 0 193 183 207 601.0 0 207 184 223 601.0 0 223 185 175 601.0 0 175 186 161 601.0 0 161 187 300 601.0 0 300 188 368 601.0 0 368 189 409 601.0 0 409 190 145 601.0 0 145 191 120 601.0 0 120 192 143 601.0 0 143 193 166 601.0 0 166 194 173 601.0 0 173 195 157 601.0 0 157 196 132 601.0 0 132 197 115 601.0 0 115 198 131 601.0 0 131 199 139 601.0 0 139 200 167 601.0 0 167 201 128 601.0 0 128 202 123 601.0 0 123 203 184 601.0 0 184 204 237 601.0 0 237 205 235 601.0 0 235 206 134 601.0 0 134 207 102 601.0 0 102 208 148 601.0 0 148 209 175 601.0 0 175 210 149 601.0 0 149 211 175 601.0 0 175 212 157 601.0 0 157 213 232 601.0 0 232 214 635 601.0 0 635 215 909 601.0 0 909 216 1671 601.0 0 1671 217 1714 601.0 0 1714 218 316 601.0 0 316 219 392 601.0 0 392 220 264 601.0 0 264 221 263 601.0 0 263 222 236 601.0 0 236 223 294 601.0 0 294 224 363 601.0 0 363 225 692 601.0 0 692 226 2529 601.0 0 2529 227 6227 601.0 0 6227 228 11242 601.0 0 11242 229 10274 601.0 0 10274 230 1572 601.0 0 1572 231 1116 601.0 0 1116 232 1965 601.0 0 1965 233 1280 601.0 0 1280 234 167 601.0 0 167 235 161 601.0 0 161 236 163 601.0 0 163 237 254 601.0 0 254 238 276 601.0 0 276 239 380 601.0 0 380 240 267 601.0 0 267 241 184 601.0 0 184 242 114 601.0 0 114 243 127 601.0 0 127 244 146 601.0 0 146 245 153 601.0 0 153 246 196 601.0 0 196 247 189 601.0 0 189 248 259 601.0 0 259 249 290 601.0 0 290 250 199 601.0 0 199 251 153 601.0 0 153 252 127 601.0 0 127 253 134 601.0 0 134 254 254 601.0 0 254 255 217 601.0 0 217 256 254 601.0 0 254 257 359 601.0 0 359 258 336 601.0 0 336 259 543 601.0 0 543 260 381 601.0 0 381 261 301 601.0 0 301 262 321 601.0 0 321 263 397 601.0 0 397 264 917 601.0 0 917 265 2592 601.0 0 2592 266 5096 601.0 0 5096 267 5686 601.0 0 5686 268 4008 601.0 0 4008 269 617 601.0 0 617 270 488 601.0 0 488 271 445 601.0 0 445 272 424 601.0 0 424 273 470 601.0 0 470 274 492 601.0 0 492 275 631 601.0 0 631 276 579 601.0 0 579 277 263 601.0 0 263 278 341 601.0 0 341 279 519 601.0 0 519 280 455 601.0 0 455 281 419 601.0 0 419 282 223 601.0 0 223 283 214 601.0 0 214 284 227 601.0 0 227 285 341 601.0 0 341 286 524 601.0 0 524 287 576 601.0 0 576 288 344 601.0 0 344 289 263 601.0 0 263 290 358 601.0 0 358 291 2724 601.0 0 2724 292 1719 601.0 0 1719 293 1685 601.0 0 1685 294 236 601.0 0 236 295 182 601.0 0 182 296 117 601.0 0 117 297 141 601.0 0 141 298 133 601.0 0 133 299 172 601.0 0 172 300 223 601.0 0 223 301 551 601.0 0 551 Finished in 8.91 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 175,054 Reads with adapters: 69,421 (39.7%) Reads that were too short: 62 (0.0%) Reads written (passing filters): 69,359 (39.6%) Total basepairs processed: 48,396,279 bp Total written (filtered): 18,831,779 bp (38.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 69421 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 16 0.0 3 0 0 0 12 4 18 50 0.0 3 0 0 28 12 10 19 1045 0.0 3 0 743 145 100 57 20 65901 0.0 4 59403 4869 1130 384 115 21 197 0.0 4 28 132 21 9 7 22 25 0.0 4 2 1 15 3 4 23 1131 0.0 4 647 47 16 283 138 24 8 0.0 4 4 2 0 0 2 26 3 0.0 4 2 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 3 0.0 4 0 0 0 0 3 29 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 1 36 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 1 67 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 1 117 1 0.0 4 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 15 0.0 4 0 0 0 0 15 127 878 0.0 4 0 0 0 0 878 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 135 63 0.0 4 0 0 0 0 63 138 1 0.0 4 0 0 1 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 156 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 1 188 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 1 1 216 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 1 8 229 4 0.0 4 0 0 0 1 3 230 4 0.0 4 0 0 0 0 4 234 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 1 2 237 1 0.0 4 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 3 0.0 4 0 0 0 0 3 262 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 5 0.0 4 0 0 0 0 5 286 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 294 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.49 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 69,359 Reads with adapters: 12,806 (18.5%) Reads written (passing filters): 69,359 (100.0%) Total basepairs processed: 18,831,779 bp Total written (filtered): 18,691,427 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 12806 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.8% C: 11.0% G: 81.9% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 181 1083.7 0 181 4 95 270.9 0 95 5 1 67.7 0 1 6 1 16.9 0 1 7 3 4.2 0 3 8 3 1.1 0 3 9 284 0.3 0 23 261 10 2597 0.1 1 205 2392 11 9211 0.0 1 347 8864 12 143 0.0 1 4 139 13 19 0.0 1 0 19 14 13 0.0 1 0 13 15 4 0.0 1 0 4 18 2 0.0 1 0 2 19 224 0.0 1 148 76 20 8 0.0 1 4 4 23 1 0.0 1 0 1 44 1 0.0 1 1 150 2 0.0 1 1 1 151 3 0.0 1 1 2 152 1 0.0 1 1 160 1 0.0 1 1 209 6 0.0 1 4 2 225 1 0.0 1 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.