This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.40 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 220,899 (9.0%) Reads that were too short: 43,340 (1.8%) Reads written (passing filters): 177,559 (7.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 50,012,540 bp (6.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 220899 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1973 601.0 0 1973 7 70873 601.0 0 70873 8 85018 601.0 0 85018 9 97 601.0 0 97 10 90 601.0 0 90 11 88 601.0 0 88 12 64 601.0 0 64 13 64 601.0 0 64 14 80 601.0 0 80 15 74 601.0 0 74 16 61 601.0 0 61 17 94 601.0 0 94 18 63 601.0 0 63 19 82 601.0 0 82 20 76 601.0 0 76 21 49 601.0 0 49 22 55 601.0 0 55 23 71 601.0 0 71 24 66 601.0 0 66 25 73 601.0 0 73 26 69 601.0 0 69 27 67 601.0 0 67 28 61 601.0 0 61 29 59 601.0 0 59 30 71 601.0 0 71 31 88 601.0 0 88 32 75 601.0 0 75 33 53 601.0 0 53 34 114 601.0 0 114 35 73 601.0 0 73 36 67 601.0 0 67 37 79 601.0 0 79 38 90 601.0 0 90 39 65 601.0 0 65 40 63 601.0 0 63 41 61 601.0 0 61 42 65 601.0 0 65 43 69 601.0 0 69 44 117 601.0 0 117 45 101 601.0 0 101 46 62 601.0 0 62 47 68 601.0 0 68 48 86 601.0 0 86 49 76 601.0 0 76 50 68 601.0 0 68 51 71 601.0 0 71 52 100 601.0 0 100 53 88 601.0 0 88 54 75 601.0 0 75 55 83 601.0 0 83 56 96 601.0 0 96 57 99 601.0 0 99 58 61 601.0 0 61 59 118 601.0 0 118 60 97 601.0 0 97 61 257 601.0 0 257 62 79 601.0 0 79 63 101 601.0 0 101 64 88 601.0 0 88 65 102 601.0 0 102 66 69 601.0 0 69 67 64 601.0 0 64 68 80 601.0 0 80 69 73 601.0 0 73 70 90 601.0 0 90 71 100 601.0 0 100 72 123 601.0 0 123 73 118 601.0 0 118 74 160 601.0 0 160 75 86 601.0 0 86 76 94 601.0 0 94 77 77 601.0 0 77 78 113 601.0 0 113 79 114 601.0 0 114 80 119 601.0 0 119 81 105 601.0 0 105 82 118 601.0 0 118 83 115 601.0 0 115 84 127 601.0 0 127 85 118 601.0 0 118 86 120 601.0 0 120 87 97 601.0 0 97 88 103 601.0 0 103 89 87 601.0 0 87 90 80 601.0 0 80 91 83 601.0 0 83 92 92 601.0 0 92 93 70 601.0 0 70 94 74 601.0 0 74 95 124 601.0 0 124 96 92 601.0 0 92 97 70 601.0 0 70 98 95 601.0 0 95 99 150 601.0 0 150 100 97 601.0 0 97 101 108 601.0 0 108 102 111 601.0 0 111 103 118 601.0 0 118 104 108 601.0 0 108 105 70 601.0 0 70 106 89 601.0 0 89 107 107 601.0 0 107 108 86 601.0 0 86 109 83 601.0 0 83 110 94 601.0 0 94 111 84 601.0 0 84 112 84 601.0 0 84 113 80 601.0 0 80 114 101 601.0 0 101 115 82 601.0 0 82 116 104 601.0 0 104 117 88 601.0 0 88 118 88 601.0 0 88 119 66 601.0 0 66 120 80 601.0 0 80 121 89 601.0 0 89 122 93 601.0 0 93 123 100 601.0 0 100 124 184 601.0 0 184 125 204 601.0 0 204 126 178 601.0 0 178 127 96 601.0 0 96 128 97 601.0 0 97 129 78 601.0 0 78 130 85 601.0 0 85 131 112 601.0 0 112 132 86 601.0 0 86 133 97 601.0 0 97 134 151 601.0 0 151 135 214 601.0 0 214 136 87 601.0 0 87 137 106 601.0 0 106 138 92 601.0 0 92 139 236 601.0 0 236 140 82 601.0 0 82 141 84 601.0 0 84 142 86 601.0 0 86 143 67 601.0 0 67 144 86 601.0 0 86 145 95 601.0 0 95 146 132 601.0 0 132 147 188 601.0 0 188 148 103 601.0 0 103 149 258 601.0 0 258 150 104 601.0 0 104 151 103 601.0 0 103 152 80 601.0 0 80 153 94 601.0 0 94 154 99 601.0 0 99 155 88 601.0 0 88 156 98 601.0 0 98 157 103 601.0 0 103 158 160 601.0 0 160 159 106 601.0 0 106 160 141 601.0 0 141 161 87 601.0 0 87 162 119 601.0 0 119 163 96 601.0 0 96 164 87 601.0 0 87 165 108 601.0 0 108 166 99 601.0 0 99 167 159 601.0 0 159 168 97 601.0 0 97 169 150 601.0 0 150 170 159 601.0 0 159 171 231 601.0 0 231 172 154 601.0 0 154 173 114 601.0 0 114 174 106 601.0 0 106 175 84 601.0 0 84 176 101 601.0 0 101 177 83 601.0 0 83 178 111 601.0 0 111 179 101 601.0 0 101 180 108 601.0 0 108 181 117 601.0 0 117 182 81 601.0 0 81 183 111 601.0 0 111 184 111 601.0 0 111 185 109 601.0 0 109 186 118 601.0 0 118 187 144 601.0 0 144 188 209 601.0 0 209 189 170 601.0 0 170 190 117 601.0 0 117 191 107 601.0 0 107 192 136 601.0 0 136 193 111 601.0 0 111 194 117 601.0 0 117 195 158 601.0 0 158 196 114 601.0 0 114 197 121 601.0 0 121 198 126 601.0 0 126 199 117 601.0 0 117 200 135 601.0 0 135 201 113 601.0 0 113 202 107 601.0 0 107 203 160 601.0 0 160 204 196 601.0 0 196 205 162 601.0 0 162 206 119 601.0 0 119 207 89 601.0 0 89 208 110 601.0 0 110 209 117 601.0 0 117 210 146 601.0 0 146 211 97 601.0 0 97 212 116 601.0 0 116 213 146 601.0 0 146 214 352 601.0 0 352 215 612 601.0 0 612 216 820 601.0 0 820 217 583 601.0 0 583 218 190 601.0 0 190 219 184 601.0 0 184 220 125 601.0 0 125 221 103 601.0 0 103 222 146 601.0 0 146 223 186 601.0 0 186 224 211 601.0 0 211 225 307 601.0 0 307 226 1188 601.0 0 1188 227 3695 601.0 0 3695 228 4723 601.0 0 4723 229 6105 601.0 0 6105 230 837 601.0 0 837 231 827 601.0 0 827 232 1587 601.0 0 1587 233 1330 601.0 0 1330 234 108 601.0 0 108 235 86 601.0 0 86 236 109 601.0 0 109 237 121 601.0 0 121 238 152 601.0 0 152 239 198 601.0 0 198 240 173 601.0 0 173 241 121 601.0 0 121 242 107 601.0 0 107 243 89 601.0 0 89 244 116 601.0 0 116 245 110 601.0 0 110 246 141 601.0 0 141 247 131 601.0 0 131 248 197 601.0 0 197 249 198 601.0 0 198 250 159 601.0 0 159 251 120 601.0 0 120 252 84 601.0 0 84 253 95 601.0 0 95 254 153 601.0 0 153 255 137 601.0 0 137 256 158 601.0 0 158 257 146 601.0 0 146 258 141 601.0 0 141 259 218 601.0 0 218 260 176 601.0 0 176 261 196 601.0 0 196 262 158 601.0 0 158 263 177 601.0 0 177 264 338 601.0 0 338 265 798 601.0 0 798 266 1657 601.0 0 1657 267 1920 601.0 0 1920 268 1435 601.0 0 1435 269 230 601.0 0 230 270 205 601.0 0 205 271 164 601.0 0 164 272 144 601.0 0 144 273 180 601.0 0 180 274 221 601.0 0 221 275 269 601.0 0 269 276 318 601.0 0 318 277 166 601.0 0 166 278 183 601.0 0 183 279 215 601.0 0 215 280 232 601.0 0 232 281 176 601.0 0 176 282 221 601.0 0 221 283 163 601.0 0 163 284 150 601.0 0 150 285 151 601.0 0 151 286 196 601.0 0 196 287 243 601.0 0 243 288 174 601.0 0 174 289 183 601.0 0 183 290 145 601.0 0 145 291 813 601.0 0 813 292 541 601.0 0 541 293 483 601.0 0 483 294 139 601.0 0 139 295 101 601.0 0 101 296 110 601.0 0 110 297 98 601.0 0 98 298 109 601.0 0 109 299 132 601.0 0 132 300 154 601.0 0 154 301 232 601.0 0 232 Finished in 8.09 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 177,559 Reads with adapters: 70,095 (39.5%) Reads that were too short: 67 (0.0%) Reads written (passing filters): 70,028 (39.4%) Total basepairs processed: 50,012,540 bp Total written (filtered): 19,389,390 bp (38.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 70095 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 7 2 16 445 0.0 3 0 434 11 17 69294 0.0 3 67763 1486 42 3 18 124 0.0 3 11 112 1 19 32 0.0 3 0 1 26 5 20 50 0.0 3 0 0 0 50 22 2 0.0 3 1 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 6 0.0 3 0 0 4 2 37 1 0.0 3 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 9 0.0 3 0 0 0 9 70 1 0.0 3 0 0 0 1 77 6 0.0 3 0 0 0 6 79 1 0.0 3 0 0 0 1 80 32 0.0 3 0 0 0 32 87 2 0.0 3 1 0 0 1 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 2 0.0 3 2 116 4 0.0 3 3 1 117 1 0.0 3 0 0 0 1 118 2 0.0 3 1 0 1 119 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 184 4 0.0 3 0 0 0 4 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 210 1 0.0 3 1 212 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 25 0.0 3 0 0 0 25 221 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 1 0.0 3 0 0 0 1 282 4 0.0 3 0 0 0 4 283 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.46 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 70,028 Reads with adapters: 674 (1.0%) Reads written (passing filters): 70,028 (100.0%) Total basepairs processed: 19,389,390 bp Total written (filtered): 19,387,343 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 674 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.6% C: 12.5% G: 5.5% T: 8.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 654 1094.2 0 654 4 16 273.5 0 16 5 3 68.4 0 3 6 1 17.1 0 1