This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.07 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 55,268 (2.2%) Reads that were too short: 7,008 (0.3%) Reads written (passing filters): 48,260 (2.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 11,357,413 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 55268 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 704 601.0 0 704 7 10960 601.0 0 10960 8 310 601.0 0 310 9 11716 601.0 0 11716 10 7 601.0 0 7 11 6 601.0 0 6 12 3 601.0 0 3 13 3 601.0 0 3 14 1 601.0 0 1 15 4 601.0 0 4 16 1 601.0 0 1 17 1 601.0 0 1 19 3 601.0 0 3 20 3 601.0 0 3 21 3 601.0 0 3 22 4 601.0 0 4 23 20 601.0 0 20 24 14 601.0 0 14 25 59 601.0 0 59 26 16 601.0 0 16 27 16 601.0 0 16 28 2 601.0 0 2 29 8 601.0 0 8 30 7 601.0 0 7 31 2 601.0 0 2 32 5 601.0 0 5 33 25 601.0 0 25 34 56 601.0 0 56 35 119 601.0 0 119 36 2 601.0 0 2 37 10 601.0 0 10 38 4 601.0 0 4 39 10 601.0 0 10 40 17 601.0 0 17 41 12 601.0 0 12 42 7 601.0 0 7 43 5 601.0 0 5 44 7 601.0 0 7 45 3 601.0 0 3 46 14 601.0 0 14 47 10 601.0 0 10 48 6 601.0 0 6 49 8 601.0 0 8 50 8 601.0 0 8 51 5 601.0 0 5 52 4 601.0 0 4 53 8 601.0 0 8 54 6 601.0 0 6 55 5 601.0 0 5 56 4 601.0 0 4 57 15 601.0 0 15 58 6 601.0 0 6 59 6 601.0 0 6 60 9 601.0 0 9 61 5 601.0 0 5 62 2 601.0 0 2 63 4 601.0 0 4 64 13 601.0 0 13 65 4 601.0 0 4 66 6 601.0 0 6 67 4 601.0 0 4 68 8 601.0 0 8 69 2 601.0 0 2 70 9 601.0 0 9 71 3 601.0 0 3 72 3 601.0 0 3 73 6 601.0 0 6 74 9 601.0 0 9 75 7 601.0 0 7 76 20 601.0 0 20 77 27 601.0 0 27 78 58 601.0 0 58 79 38 601.0 0 38 80 17 601.0 0 17 81 29 601.0 0 29 82 15 601.0 0 15 83 23 601.0 0 23 84 6 601.0 0 6 85 14 601.0 0 14 86 30 601.0 0 30 87 20 601.0 0 20 88 22 601.0 0 22 89 28 601.0 0 28 90 15 601.0 0 15 91 15 601.0 0 15 92 14 601.0 0 14 93 22 601.0 0 22 94 34 601.0 0 34 95 37 601.0 0 37 96 46 601.0 0 46 97 98 601.0 0 98 98 146 601.0 0 146 99 1371 601.0 0 1371 100 681 601.0 0 681 101 480 601.0 0 480 102 72 601.0 0 72 103 26 601.0 0 26 104 30 601.0 0 30 105 17 601.0 0 17 106 28 601.0 0 28 107 41 601.0 0 41 108 70 601.0 0 70 109 134 601.0 0 134 110 194 601.0 0 194 111 5116 601.0 0 5116 112 2010 601.0 0 2010 113 1283 601.0 0 1283 114 1160 601.0 0 1160 115 482 601.0 0 482 116 9 601.0 0 9 117 14 601.0 0 14 118 12 601.0 0 12 119 15 601.0 0 15 120 15 601.0 0 15 121 19 601.0 0 19 122 9 601.0 0 9 123 21 601.0 0 21 124 15 601.0 0 15 125 26 601.0 0 26 126 23 601.0 0 23 127 132 601.0 0 132 128 36 601.0 0 36 129 262 601.0 0 262 130 121 601.0 0 121 131 96 601.0 0 96 132 44 601.0 0 44 133 53 601.0 0 53 134 41 601.0 0 41 135 228 601.0 0 228 136 259 601.0 0 259 137 132 601.0 0 132 138 40 601.0 0 40 139 70 601.0 0 70 140 67 601.0 0 67 141 120 601.0 0 120 142 2186 601.0 0 2186 143 3379 601.0 0 3379 144 897 601.0 0 897 145 281 601.0 0 281 146 28 601.0 0 28 147 37 601.0 0 37 148 16 601.0 0 16 149 26 601.0 0 26 150 13 601.0 0 13 151 12 601.0 0 12 152 17 601.0 0 17 153 8 601.0 0 8 154 13 601.0 0 13 155 15 601.0 0 15 156 28 601.0 0 28 157 30 601.0 0 30 158 49 601.0 0 49 159 19 601.0 0 19 160 37 601.0 0 37 161 28 601.0 0 28 162 47 601.0 0 47 163 19 601.0 0 19 164 17 601.0 0 17 165 32 601.0 0 32 166 34 601.0 0 34 167 18 601.0 0 18 168 16 601.0 0 16 169 20 601.0 0 20 170 7 601.0 0 7 171 17 601.0 0 17 172 15 601.0 0 15 173 5 601.0 0 5 174 13 601.0 0 13 175 9 601.0 0 9 176 16 601.0 0 16 177 43 601.0 0 43 178 35 601.0 0 35 179 19 601.0 0 19 180 17 601.0 0 17 181 19 601.0 0 19 182 15 601.0 0 15 183 20 601.0 0 20 184 15 601.0 0 15 185 26 601.0 0 26 186 182 601.0 0 182 187 15 601.0 0 15 188 59 601.0 0 59 189 24 601.0 0 24 190 14 601.0 0 14 191 19 601.0 0 19 192 2 601.0 0 2 193 11 601.0 0 11 194 12 601.0 0 12 195 14 601.0 0 14 196 15 601.0 0 15 197 20 601.0 0 20 198 9 601.0 0 9 199 15 601.0 0 15 200 9 601.0 0 9 201 25 601.0 0 25 202 26 601.0 0 26 203 25 601.0 0 25 204 22 601.0 0 22 205 15 601.0 0 15 206 24 601.0 0 24 207 14 601.0 0 14 208 11 601.0 0 11 209 32 601.0 0 32 210 21 601.0 0 21 211 19 601.0 0 19 212 19 601.0 0 19 213 23 601.0 0 23 214 31 601.0 0 31 215 14 601.0 0 14 216 27 601.0 0 27 217 17 601.0 0 17 218 15 601.0 0 15 219 26 601.0 0 26 220 20 601.0 0 20 221 47 601.0 0 47 222 40 601.0 0 40 223 65 601.0 0 65 224 77 601.0 0 77 225 52 601.0 0 52 226 55 601.0 0 55 227 31 601.0 0 31 228 27 601.0 0 27 229 35 601.0 0 35 230 24 601.0 0 24 231 27 601.0 0 27 232 32 601.0 0 32 233 46 601.0 0 46 234 40 601.0 0 40 235 46 601.0 0 46 236 22 601.0 0 22 237 39 601.0 0 39 238 46 601.0 0 46 239 42 601.0 0 42 240 35 601.0 0 35 241 82 601.0 0 82 242 68 601.0 0 68 243 78 601.0 0 78 244 51 601.0 0 51 245 31 601.0 0 31 246 17 601.0 0 17 247 22 601.0 0 22 248 19 601.0 0 19 249 20 601.0 0 20 250 49 601.0 0 49 251 39 601.0 0 39 252 38 601.0 0 38 253 50 601.0 0 50 254 41 601.0 0 41 255 54 601.0 0 54 256 121 601.0 0 121 257 136 601.0 0 136 258 104 601.0 0 104 259 84 601.0 0 84 260 49 601.0 0 49 261 70 601.0 0 70 262 159 601.0 0 159 263 257 601.0 0 257 264 175 601.0 0 175 265 62 601.0 0 62 266 20 601.0 0 20 267 27 601.0 0 27 268 34 601.0 0 34 269 24 601.0 0 24 270 38 601.0 0 38 271 52 601.0 0 52 272 38 601.0 0 38 273 41 601.0 0 41 274 46 601.0 0 46 275 239 601.0 0 239 276 119 601.0 0 119 277 289 601.0 0 289 278 68 601.0 0 68 279 56 601.0 0 56 280 108 601.0 0 108 281 61 601.0 0 61 282 182 601.0 0 182 283 205 601.0 0 205 284 448 601.0 0 448 285 136 601.0 0 136 286 49 601.0 0 49 287 37 601.0 0 37 288 31 601.0 0 31 289 60 601.0 0 60 290 206 601.0 0 206 291 228 601.0 0 228 292 119 601.0 0 119 293 109 601.0 0 109 294 278 601.0 0 278 295 201 601.0 0 201 296 53 601.0 0 53 297 74 601.0 0 74 298 55 601.0 0 55 299 56 601.0 0 56 300 78 601.0 0 78 301 38 601.0 0 38 Finished in 2.39 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 48,260 Reads with adapters: 11,153 (23.1%) Reads that were too short: 46 (0.1%) Reads written (passing filters): 11,107 (23.0%) Total basepairs processed: 11,357,413 bp Total written (filtered): 3,010,552 bp (26.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11153 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 1 2 2 19 97 0.0 3 0 79 8 7 3 20 10645 0.0 4 9728 725 140 34 18 21 23 0.0 4 4 17 2 22 4 0.0 4 1 0 2 0 1 23 27 0.0 4 1 0 0 17 9 25 1 0.0 4 0 0 0 0 1 35 14 0.0 4 0 0 0 0 14 55 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 122 4 0.0 4 0 0 0 0 4 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 12 0.0 4 0 0 0 0 12 127 272 0.0 4 0 0 0 0 272 128 6 0.0 4 0 0 0 0 6 129 7 0.0 4 0 0 0 0 7 133 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 229 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.39 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 11,107 Reads with adapters: 569 (5.1%) Reads written (passing filters): 11,107 (100.0%) Total basepairs processed: 3,010,552 bp Total written (filtered): 3,004,207 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 569 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.4% C: 16.7% G: 69.8% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 76 173.5 0 76 4 11 43.4 0 11 9 3 0.0 0 0 3 10 8 0.0 1 0 8 11 242 0.0 1 9 233 12 222 0.0 1 8 214 13 2 0.0 1 0 2 14 1 0.0 1 0 1 15 1 0.0 1 0 1 152 1 0.0 1 0 1 210 1 0.0 1 1 223 1 0.0 1 1