This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.09 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 51,718 (2.1%) Reads that were too short: 4,531 (0.2%) Reads written (passing filters): 47,187 (1.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 11,289,721 bp (1.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 51718 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 737 601.0 0 737 7 14742 601.0 0 14742 8 228 601.0 0 228 9 9689 601.0 0 9689 10 8 601.0 0 8 11 2 601.0 0 2 12 3 601.0 0 3 14 4 601.0 0 4 15 3 601.0 0 3 17 1 601.0 0 1 18 3 601.0 0 3 19 1 601.0 0 1 21 1 601.0 0 1 22 1 601.0 0 1 23 1 601.0 0 1 25 1 601.0 0 1 27 2 601.0 0 2 28 6 601.0 0 6 29 6 601.0 0 6 30 1 601.0 0 1 31 1 601.0 0 1 32 1 601.0 0 1 33 4 601.0 0 4 34 2 601.0 0 2 35 6 601.0 0 6 36 3 601.0 0 3 37 4 601.0 0 4 38 1 601.0 0 1 39 4 601.0 0 4 40 3 601.0 0 3 41 8 601.0 0 8 42 2 601.0 0 2 43 8 601.0 0 8 44 1 601.0 0 1 45 7 601.0 0 7 46 18 601.0 0 18 47 7 601.0 0 7 48 5 601.0 0 5 49 2 601.0 0 2 50 8 601.0 0 8 51 9 601.0 0 9 52 6 601.0 0 6 53 6 601.0 0 6 54 7 601.0 0 7 55 2 601.0 0 2 56 1 601.0 0 1 57 16 601.0 0 16 58 4 601.0 0 4 59 4 601.0 0 4 60 3 601.0 0 3 61 2 601.0 0 2 62 3 601.0 0 3 63 2 601.0 0 2 64 2 601.0 0 2 65 3 601.0 0 3 66 5 601.0 0 5 67 3 601.0 0 3 68 7 601.0 0 7 69 3 601.0 0 3 70 5 601.0 0 5 71 3 601.0 0 3 72 4 601.0 0 4 73 2 601.0 0 2 74 3 601.0 0 3 75 2 601.0 0 2 76 4 601.0 0 4 77 10 601.0 0 10 78 26 601.0 0 26 79 8 601.0 0 8 80 3 601.0 0 3 81 4 601.0 0 4 82 12 601.0 0 12 83 13 601.0 0 13 84 1 601.0 0 1 85 11 601.0 0 11 86 23 601.0 0 23 87 20 601.0 0 20 88 13 601.0 0 13 89 18 601.0 0 18 90 6 601.0 0 6 91 13 601.0 0 13 92 11 601.0 0 11 93 23 601.0 0 23 94 16 601.0 0 16 95 22 601.0 0 22 96 24 601.0 0 24 97 37 601.0 0 37 98 74 601.0 0 74 99 371 601.0 0 371 100 197 601.0 0 197 101 123 601.0 0 123 102 30 601.0 0 30 103 27 601.0 0 27 104 37 601.0 0 37 105 20 601.0 0 20 106 39 601.0 0 39 107 29 601.0 0 29 108 65 601.0 0 65 109 118 601.0 0 118 110 148 601.0 0 148 111 4939 601.0 0 4939 112 2174 601.0 0 2174 113 1353 601.0 0 1353 114 1212 601.0 0 1212 115 484 601.0 0 484 116 8 601.0 0 8 117 10 601.0 0 10 118 19 601.0 0 19 119 15 601.0 0 15 120 13 601.0 0 13 121 13 601.0 0 13 122 12 601.0 0 12 123 5 601.0 0 5 124 6 601.0 0 6 125 21 601.0 0 21 126 13 601.0 0 13 127 140 601.0 0 140 128 30 601.0 0 30 129 168 601.0 0 168 130 78 601.0 0 78 131 48 601.0 0 48 132 37 601.0 0 37 133 39 601.0 0 39 134 22 601.0 0 22 135 247 601.0 0 247 136 251 601.0 0 251 137 130 601.0 0 130 138 23 601.0 0 23 139 53 601.0 0 53 140 68 601.0 0 68 141 120 601.0 0 120 142 2378 601.0 0 2378 143 3760 601.0 0 3760 144 1003 601.0 0 1003 145 310 601.0 0 310 146 11 601.0 0 11 147 21 601.0 0 21 148 7 601.0 0 7 149 16 601.0 0 16 150 6 601.0 0 6 151 8 601.0 0 8 152 17 601.0 0 17 153 3 601.0 0 3 154 3 601.0 0 3 155 3 601.0 0 3 156 12 601.0 0 12 157 10 601.0 0 10 158 11 601.0 0 11 159 10 601.0 0 10 160 14 601.0 0 14 161 15 601.0 0 15 162 16 601.0 0 16 163 10 601.0 0 10 164 13 601.0 0 13 165 9 601.0 0 9 166 13 601.0 0 13 167 6 601.0 0 6 168 7 601.0 0 7 169 5 601.0 0 5 170 4 601.0 0 4 171 7 601.0 0 7 172 13 601.0 0 13 173 3 601.0 0 3 174 9 601.0 0 9 175 8 601.0 0 8 176 14 601.0 0 14 177 17 601.0 0 17 178 23 601.0 0 23 179 10 601.0 0 10 180 5 601.0 0 5 181 7 601.0 0 7 182 6 601.0 0 6 183 12 601.0 0 12 184 11 601.0 0 11 185 11 601.0 0 11 186 156 601.0 0 156 187 14 601.0 0 14 188 76 601.0 0 76 189 15 601.0 0 15 190 12 601.0 0 12 191 4 601.0 0 4 192 6 601.0 0 6 193 8 601.0 0 8 194 7 601.0 0 7 195 7 601.0 0 7 196 8 601.0 0 8 197 4 601.0 0 4 198 5 601.0 0 5 199 8 601.0 0 8 200 5 601.0 0 5 201 6 601.0 0 6 202 9 601.0 0 9 203 11 601.0 0 11 204 7 601.0 0 7 205 3 601.0 0 3 206 9 601.0 0 9 207 10 601.0 0 10 208 13 601.0 0 13 209 13 601.0 0 13 210 17 601.0 0 17 211 10 601.0 0 10 212 10 601.0 0 10 213 13 601.0 0 13 214 10 601.0 0 10 215 17 601.0 0 17 216 15 601.0 0 15 217 9 601.0 0 9 218 12 601.0 0 12 219 10 601.0 0 10 220 14 601.0 0 14 221 20 601.0 0 20 222 13 601.0 0 13 223 23 601.0 0 23 224 71 601.0 0 71 225 71 601.0 0 71 226 42 601.0 0 42 227 7 601.0 0 7 228 4 601.0 0 4 229 8 601.0 0 8 230 7 601.0 0 7 231 7 601.0 0 7 232 16 601.0 0 16 233 26 601.0 0 26 234 18 601.0 0 18 235 16 601.0 0 16 236 18 601.0 0 18 237 25 601.0 0 25 238 15 601.0 0 15 239 29 601.0 0 29 240 21 601.0 0 21 241 29 601.0 0 29 242 27 601.0 0 27 243 34 601.0 0 34 244 29 601.0 0 29 245 18 601.0 0 18 246 8 601.0 0 8 247 17 601.0 0 17 248 8 601.0 0 8 249 13 601.0 0 13 250 30 601.0 0 30 251 25 601.0 0 25 252 21 601.0 0 21 253 13 601.0 0 13 254 16 601.0 0 16 255 33 601.0 0 33 256 55 601.0 0 55 257 30 601.0 0 30 258 20 601.0 0 20 259 27 601.0 0 27 260 23 601.0 0 23 261 24 601.0 0 24 262 66 601.0 0 66 263 149 601.0 0 149 264 62 601.0 0 62 265 27 601.0 0 27 266 24 601.0 0 24 267 21 601.0 0 21 268 12 601.0 0 12 269 14 601.0 0 14 270 22 601.0 0 22 271 24 601.0 0 24 272 16 601.0 0 16 273 21 601.0 0 21 274 33 601.0 0 33 275 238 601.0 0 238 276 86 601.0 0 86 277 281 601.0 0 281 278 43 601.0 0 43 279 28 601.0 0 28 280 75 601.0 0 75 281 46 601.0 0 46 282 96 601.0 0 96 283 111 601.0 0 111 284 199 601.0 0 199 285 49 601.0 0 49 286 21 601.0 0 21 287 22 601.0 0 22 288 14 601.0 0 14 289 49 601.0 0 49 290 244 601.0 0 244 291 243 601.0 0 243 292 107 601.0 0 107 293 61 601.0 0 61 294 349 601.0 0 349 295 274 601.0 0 274 296 33 601.0 0 33 297 46 601.0 0 46 298 42 601.0 0 42 299 55 601.0 0 55 300 38 601.0 0 38 301 21 601.0 0 21 Finished in 2.10 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 47,187 Reads with adapters: 10,775 (22.8%) Reads that were too short: 110 (0.2%) Reads written (passing filters): 10,665 (22.6%) Total basepairs processed: 11,289,721 bp Total written (filtered): 2,921,187 bp (25.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10775 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 81 0.0 3 0 81 17 9905 0.0 3 9702 198 5 18 21 0.0 3 3 17 1 19 398 0.0 3 377 17 4 20 174 0.0 3 161 5 0 8 23 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 29 54 0.0 3 0 0 0 54 32 6 0.0 3 0 0 0 6 40 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 62 4 0.0 3 0 0 0 4 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 110 1 0.0 3 0 0 0 1 114 2 0.0 3 1 0 0 1 117 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 0 3 142 3 0.0 3 0 0 0 3 143 2 0.0 3 0 0 0 2 144 14 0.0 3 0 0 0 14 145 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 75 0.0 3 0 0 2 73 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 10,665 Reads with adapters: 172 (1.6%) Reads written (passing filters): 10,665 (100.0%) Total basepairs processed: 2,921,187 bp Total written (filtered): 2,920,659 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 172 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.4% C: 5.2% G: 19.2% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 160 166.6 0 160 4 12 41.7 0 12