This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.66 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 165,033 (6.7%) Reads that were too short: 13,634 (0.6%) Reads written (passing filters): 151,399 (6.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 41,836,182 bp (5.6%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 165033 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1690 601.0 0 1690 7 67842 601.0 0 67842 8 2074 601.0 0 2074 9 55757 601.0 0 55757 10 97 601.0 0 97 11 65 601.0 0 65 12 66 601.0 0 66 13 72 601.0 0 72 14 85 601.0 0 85 15 46 601.0 0 46 16 53 601.0 0 53 17 54 601.0 0 54 18 77 601.0 0 77 19 38 601.0 0 38 20 48 601.0 0 48 21 45 601.0 0 45 22 28 601.0 0 28 23 37 601.0 0 37 24 45 601.0 0 45 25 45 601.0 0 45 26 40 601.0 0 40 27 52 601.0 0 52 28 60 601.0 0 60 29 76 601.0 0 76 30 92 601.0 0 92 31 48 601.0 0 48 32 67 601.0 0 67 33 114 601.0 0 114 34 49 601.0 0 49 35 46 601.0 0 46 36 50 601.0 0 50 37 62 601.0 0 62 38 80 601.0 0 80 39 83 601.0 0 83 40 80 601.0 0 80 41 104 601.0 0 104 42 64 601.0 0 64 43 81 601.0 0 81 44 145 601.0 0 145 45 177 601.0 0 177 46 509 601.0 0 509 47 124 601.0 0 124 48 41 601.0 0 41 49 62 601.0 0 62 50 51 601.0 0 51 51 99 601.0 0 99 52 55 601.0 0 55 53 88 601.0 0 88 54 68 601.0 0 68 55 62 601.0 0 62 56 146 601.0 0 146 57 362 601.0 0 362 58 1275 601.0 0 1275 59 428 601.0 0 428 60 136 601.0 0 136 61 57 601.0 0 57 62 52 601.0 0 52 63 30 601.0 0 30 64 52 601.0 0 52 65 48 601.0 0 48 66 52 601.0 0 52 67 51 601.0 0 51 68 69 601.0 0 69 69 37 601.0 0 37 70 96 601.0 0 96 71 43 601.0 0 43 72 52 601.0 0 52 73 81 601.0 0 81 74 68 601.0 0 68 75 67 601.0 0 67 76 74 601.0 0 74 77 65 601.0 0 65 78 52 601.0 0 52 79 66 601.0 0 66 80 56 601.0 0 56 81 64 601.0 0 64 82 57 601.0 0 57 83 70 601.0 0 70 84 70 601.0 0 70 85 52 601.0 0 52 86 64 601.0 0 64 87 46 601.0 0 46 88 57 601.0 0 57 89 51 601.0 0 51 90 49 601.0 0 49 91 99 601.0 0 99 92 116 601.0 0 116 93 94 601.0 0 94 94 76 601.0 0 76 95 53 601.0 0 53 96 60 601.0 0 60 97 79 601.0 0 79 98 76 601.0 0 76 99 76 601.0 0 76 100 51 601.0 0 51 101 65 601.0 0 65 102 65 601.0 0 65 103 57 601.0 0 57 104 72 601.0 0 72 105 93 601.0 0 93 106 335 601.0 0 335 107 471 601.0 0 471 108 871 601.0 0 871 109 98 601.0 0 98 110 125 601.0 0 125 111 70 601.0 0 70 112 55 601.0 0 55 113 119 601.0 0 119 114 254 601.0 0 254 115 156 601.0 0 156 116 79 601.0 0 79 117 51 601.0 0 51 118 55 601.0 0 55 119 73 601.0 0 73 120 48 601.0 0 48 121 68 601.0 0 68 122 47 601.0 0 47 123 77 601.0 0 77 124 77 601.0 0 77 125 99 601.0 0 99 126 77 601.0 0 77 127 106 601.0 0 106 128 225 601.0 0 225 129 280 601.0 0 280 130 126 601.0 0 126 131 75 601.0 0 75 132 60 601.0 0 60 133 92 601.0 0 92 134 131 601.0 0 131 135 220 601.0 0 220 136 349 601.0 0 349 137 235 601.0 0 235 138 174 601.0 0 174 139 165 601.0 0 165 140 136 601.0 0 136 141 222 601.0 0 222 142 224 601.0 0 224 143 113 601.0 0 113 144 72 601.0 0 72 145 73 601.0 0 73 146 95 601.0 0 95 147 96 601.0 0 96 148 1220 601.0 0 1220 149 417 601.0 0 417 150 1109 601.0 0 1109 151 49 601.0 0 49 152 47 601.0 0 47 153 53 601.0 0 53 154 57 601.0 0 57 155 201 601.0 0 201 156 270 601.0 0 270 157 342 601.0 0 342 158 116 601.0 0 116 159 71 601.0 0 71 160 80 601.0 0 80 161 80 601.0 0 80 162 124 601.0 0 124 163 361 601.0 0 361 164 630 601.0 0 630 165 839 601.0 0 839 166 72 601.0 0 72 167 47 601.0 0 47 168 70 601.0 0 70 169 112 601.0 0 112 170 111 601.0 0 111 171 39 601.0 0 39 172 41 601.0 0 41 173 29 601.0 0 29 174 63 601.0 0 63 175 37 601.0 0 37 176 31 601.0 0 31 177 41 601.0 0 41 178 45 601.0 0 45 179 68 601.0 0 68 180 161 601.0 0 161 181 194 601.0 0 194 182 207 601.0 0 207 183 119 601.0 0 119 184 73 601.0 0 73 185 87 601.0 0 87 186 74 601.0 0 74 187 40 601.0 0 40 188 39 601.0 0 39 189 52 601.0 0 52 190 58 601.0 0 58 191 72 601.0 0 72 192 58 601.0 0 58 193 82 601.0 0 82 194 38 601.0 0 38 195 43 601.0 0 43 196 57 601.0 0 57 197 54 601.0 0 54 198 62 601.0 0 62 199 66 601.0 0 66 200 45 601.0 0 45 201 52 601.0 0 52 202 231 601.0 0 231 203 99 601.0 0 99 204 201 601.0 0 201 205 39 601.0 0 39 206 54 601.0 0 54 207 48 601.0 0 48 208 79 601.0 0 79 209 77 601.0 0 77 210 56 601.0 0 56 211 42 601.0 0 42 212 49 601.0 0 49 213 100 601.0 0 100 214 99 601.0 0 99 215 105 601.0 0 105 216 96 601.0 0 96 217 66 601.0 0 66 218 65 601.0 0 65 219 38 601.0 0 38 220 61 601.0 0 61 221 54 601.0 0 54 222 58 601.0 0 58 223 65 601.0 0 65 224 59 601.0 0 59 225 54 601.0 0 54 226 48 601.0 0 48 227 50 601.0 0 50 228 62 601.0 0 62 229 50 601.0 0 50 230 48 601.0 0 48 231 41 601.0 0 41 232 110 601.0 0 110 233 189 601.0 0 189 234 178 601.0 0 178 235 107 601.0 0 107 236 75 601.0 0 75 237 55 601.0 0 55 238 79 601.0 0 79 239 60 601.0 0 60 240 46 601.0 0 46 241 44 601.0 0 44 242 104 601.0 0 104 243 71 601.0 0 71 244 110 601.0 0 110 245 83 601.0 0 83 246 70 601.0 0 70 247 61 601.0 0 61 248 105 601.0 0 105 249 58 601.0 0 58 250 58 601.0 0 58 251 100 601.0 0 100 252 111 601.0 0 111 253 159 601.0 0 159 254 94 601.0 0 94 255 112 601.0 0 112 256 92 601.0 0 92 257 78 601.0 0 78 258 81 601.0 0 81 259 95 601.0 0 95 260 60 601.0 0 60 261 129 601.0 0 129 262 189 601.0 0 189 263 111 601.0 0 111 264 88 601.0 0 88 265 92 601.0 0 92 266 94 601.0 0 94 267 66 601.0 0 66 268 67 601.0 0 67 269 103 601.0 0 103 270 207 601.0 0 207 271 818 601.0 0 818 272 338 601.0 0 338 273 256 601.0 0 256 274 71 601.0 0 71 275 85 601.0 0 85 276 90 601.0 0 90 277 89 601.0 0 89 278 104 601.0 0 104 279 155 601.0 0 155 280 144 601.0 0 144 281 114 601.0 0 114 282 92 601.0 0 92 283 154 601.0 0 154 284 122 601.0 0 122 285 139 601.0 0 139 286 75 601.0 0 75 287 71 601.0 0 71 288 95 601.0 0 95 289 85 601.0 0 85 290 136 601.0 0 136 291 127 601.0 0 127 292 199 601.0 0 199 293 133 601.0 0 133 294 229 601.0 0 229 295 898 601.0 0 898 296 1117 601.0 0 1117 297 1143 601.0 0 1143 298 368 601.0 0 368 299 67 601.0 0 67 300 70 601.0 0 70 301 65 601.0 0 65 Finished in 7.85 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 151,399 Reads with adapters: 59,507 (39.3%) Reads that were too short: 73 (0.0%) Reads written (passing filters): 59,434 (39.3%) Total basepairs processed: 41,836,182 bp Total written (filtered): 16,057,760 bp (38.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 59507 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 5 2 18 42 0.0 3 0 0 22 11 9 19 599 0.0 3 0 484 52 43 20 20 57331 0.0 4 52705 3554 751 245 76 21 147 0.0 4 27 101 15 1 3 22 17 0.0 4 1 0 7 5 4 23 222 0.0 4 1 2 0 158 61 24 3 0.0 4 1 0 0 0 2 25 1 0.0 4 0 0 1 26 1 0.0 4 1 27 3 0.0 4 1 0 0 0 2 28 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 58 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 1 80 2 0.0 4 0 0 0 0 2 83 1 0.0 4 1 87 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 1 0 0 1 125 5 0.0 4 0 0 0 0 5 126 12 0.0 4 0 0 0 0 12 127 1018 0.0 4 0 0 0 0 1018 128 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 2 0.0 4 0 0 0 0 2 141 2 0.0 4 0 0 0 0 2 153 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 217 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 15 0.0 4 0 0 0 0 15 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 1 2 235 2 0.0 4 0 0 0 0 2 237 3 0.0 4 0 0 0 0 3 239 1 0.0 4 0 0 0 1 240 1 0.0 4 0 0 0 1 249 1 0.0 4 0 0 0 0 1 257 3 0.0 4 0 0 0 0 3 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 270 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 1 288 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.18 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 59,434 Reads with adapters: 14,058 (23.7%) Reads written (passing filters): 59,434 (100.0%) Total basepairs processed: 16,057,760 bp Total written (filtered): 15,904,411 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14058 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 9.7% G: 85.4% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 46 928.7 0 46 4 17 232.2 0 17 6 7 14.5 0 7 7 4 3.6 0 4 8 2 0.9 0 2 9 2773 0.2 0 261 2512 10 9140 0.1 1 642 8498 11 1896 0.0 1 72 1824 12 49 0.0 1 1 48 13 39 0.0 1 0 39 14 2 0.0 1 0 2 19 1 0.0 1 1 81 1 0.0 1 0 1 96 1 0.0 1 1 122 24 0.0 1 23 1 150 4 0.0 1 1 3 177 2 0.0 1 1 1 208 16 0.0 1 14 2 209 2 0.0 1 2 215 29 0.0 1 25 4 216 1 0.0 1 1 218 1 0.0 1 1 228 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.