This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.78 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 155,550 (6.3%) Reads that were too short: 6,931 (0.3%) Reads written (passing filters): 148,619 (6.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 41,983,718 bp (5.7%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 155550 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1024 601.0 0 1024 7 64355 601.0 0 64355 8 2211 601.0 0 2211 9 66859 601.0 0 66859 10 69 601.0 0 69 11 35 601.0 0 35 12 30 601.0 0 30 13 31 601.0 0 31 14 29 601.0 0 29 15 12 601.0 0 12 16 30 601.0 0 30 17 36 601.0 0 36 18 40 601.0 0 40 19 22 601.0 0 22 20 20 601.0 0 20 21 18 601.0 0 18 22 15 601.0 0 15 23 32 601.0 0 32 24 18 601.0 0 18 25 31 601.0 0 31 26 27 601.0 0 27 27 35 601.0 0 35 28 27 601.0 0 27 29 34 601.0 0 34 30 24 601.0 0 24 31 26 601.0 0 26 32 23 601.0 0 23 33 42 601.0 0 42 34 32 601.0 0 32 35 34 601.0 0 34 36 27 601.0 0 27 37 29 601.0 0 29 38 37 601.0 0 37 39 30 601.0 0 30 40 54 601.0 0 54 41 91 601.0 0 91 42 38 601.0 0 38 43 49 601.0 0 49 44 71 601.0 0 71 45 108 601.0 0 108 46 176 601.0 0 176 47 115 601.0 0 115 48 22 601.0 0 22 49 19 601.0 0 19 50 27 601.0 0 27 51 44 601.0 0 44 52 46 601.0 0 46 53 46 601.0 0 46 54 44 601.0 0 44 55 26 601.0 0 26 56 106 601.0 0 106 57 261 601.0 0 261 58 121 601.0 0 121 59 61 601.0 0 61 60 28 601.0 0 28 61 40 601.0 0 40 62 26 601.0 0 26 63 33 601.0 0 33 64 39 601.0 0 39 65 34 601.0 0 34 66 35 601.0 0 35 67 35 601.0 0 35 68 41 601.0 0 41 69 30 601.0 0 30 70 75 601.0 0 75 71 32 601.0 0 32 72 27 601.0 0 27 73 53 601.0 0 53 74 43 601.0 0 43 75 48 601.0 0 48 76 70 601.0 0 70 77 35 601.0 0 35 78 37 601.0 0 37 79 56 601.0 0 56 80 31 601.0 0 31 81 55 601.0 0 55 82 43 601.0 0 43 83 46 601.0 0 46 84 41 601.0 0 41 85 52 601.0 0 52 86 57 601.0 0 57 87 41 601.0 0 41 88 39 601.0 0 39 89 40 601.0 0 40 90 37 601.0 0 37 91 71 601.0 0 71 92 58 601.0 0 58 93 53 601.0 0 53 94 41 601.0 0 41 95 38 601.0 0 38 96 52 601.0 0 52 97 41 601.0 0 41 98 48 601.0 0 48 99 48 601.0 0 48 100 45 601.0 0 45 101 37 601.0 0 37 102 42 601.0 0 42 103 52 601.0 0 52 104 46 601.0 0 46 105 64 601.0 0 64 106 229 601.0 0 229 107 278 601.0 0 278 108 535 601.0 0 535 109 46 601.0 0 46 110 88 601.0 0 88 111 51 601.0 0 51 112 42 601.0 0 42 113 80 601.0 0 80 114 327 601.0 0 327 115 177 601.0 0 177 116 61 601.0 0 61 117 49 601.0 0 49 118 32 601.0 0 32 119 48 601.0 0 48 120 44 601.0 0 44 121 43 601.0 0 43 122 34 601.0 0 34 123 54 601.0 0 54 124 53 601.0 0 53 125 53 601.0 0 53 126 32 601.0 0 32 127 45 601.0 0 45 128 81 601.0 0 81 129 98 601.0 0 98 130 78 601.0 0 78 131 60 601.0 0 60 132 45 601.0 0 45 133 58 601.0 0 58 134 93 601.0 0 93 135 128 601.0 0 128 136 248 601.0 0 248 137 245 601.0 0 245 138 152 601.0 0 152 139 134 601.0 0 134 140 80 601.0 0 80 141 149 601.0 0 149 142 211 601.0 0 211 143 69 601.0 0 69 144 50 601.0 0 50 145 33 601.0 0 33 146 61 601.0 0 61 147 49 601.0 0 49 148 892 601.0 0 892 149 315 601.0 0 315 150 958 601.0 0 958 151 32 601.0 0 32 152 33 601.0 0 33 153 49 601.0 0 49 154 41 601.0 0 41 155 101 601.0 0 101 156 162 601.0 0 162 157 131 601.0 0 131 158 101 601.0 0 101 159 40 601.0 0 40 160 23 601.0 0 23 161 53 601.0 0 53 162 67 601.0 0 67 163 155 601.0 0 155 164 206 601.0 0 206 165 236 601.0 0 236 166 60 601.0 0 60 167 35 601.0 0 35 168 39 601.0 0 39 169 40 601.0 0 40 170 39 601.0 0 39 171 23 601.0 0 23 172 31 601.0 0 31 173 30 601.0 0 30 174 24 601.0 0 24 175 35 601.0 0 35 176 25 601.0 0 25 177 41 601.0 0 41 178 32 601.0 0 32 179 49 601.0 0 49 180 57 601.0 0 57 181 216 601.0 0 216 182 72 601.0 0 72 183 46 601.0 0 46 184 33 601.0 0 33 185 44 601.0 0 44 186 37 601.0 0 37 187 26 601.0 0 26 188 24 601.0 0 24 189 35 601.0 0 35 190 41 601.0 0 41 191 52 601.0 0 52 192 33 601.0 0 33 193 36 601.0 0 36 194 37 601.0 0 37 195 37 601.0 0 37 196 52 601.0 0 52 197 49 601.0 0 49 198 32 601.0 0 32 199 68 601.0 0 68 200 40 601.0 0 40 201 27 601.0 0 27 202 226 601.0 0 226 203 89 601.0 0 89 204 107 601.0 0 107 205 33 601.0 0 33 206 33 601.0 0 33 207 25 601.0 0 25 208 26 601.0 0 26 209 52 601.0 0 52 210 41 601.0 0 41 211 33 601.0 0 33 212 48 601.0 0 48 213 49 601.0 0 49 214 63 601.0 0 63 215 51 601.0 0 51 216 61 601.0 0 61 217 55 601.0 0 55 218 31 601.0 0 31 219 22 601.0 0 22 220 40 601.0 0 40 221 43 601.0 0 43 222 37 601.0 0 37 223 45 601.0 0 45 224 33 601.0 0 33 225 35 601.0 0 35 226 31 601.0 0 31 227 29 601.0 0 29 228 39 601.0 0 39 229 33 601.0 0 33 230 28 601.0 0 28 231 39 601.0 0 39 232 68 601.0 0 68 233 66 601.0 0 66 234 58 601.0 0 58 235 43 601.0 0 43 236 61 601.0 0 61 237 36 601.0 0 36 238 58 601.0 0 58 239 35 601.0 0 35 240 40 601.0 0 40 241 42 601.0 0 42 242 48 601.0 0 48 243 37 601.0 0 37 244 56 601.0 0 56 245 48 601.0 0 48 246 26 601.0 0 26 247 65 601.0 0 65 248 42 601.0 0 42 249 48 601.0 0 48 250 49 601.0 0 49 251 44 601.0 0 44 252 69 601.0 0 69 253 66 601.0 0 66 254 54 601.0 0 54 255 47 601.0 0 47 256 55 601.0 0 55 257 42 601.0 0 42 258 47 601.0 0 47 259 48 601.0 0 48 260 43 601.0 0 43 261 61 601.0 0 61 262 78 601.0 0 78 263 67 601.0 0 67 264 45 601.0 0 45 265 51 601.0 0 51 266 56 601.0 0 56 267 42 601.0 0 42 268 37 601.0 0 37 269 57 601.0 0 57 270 133 601.0 0 133 271 283 601.0 0 283 272 200 601.0 0 200 273 121 601.0 0 121 274 93 601.0 0 93 275 102 601.0 0 102 276 64 601.0 0 64 277 49 601.0 0 49 278 63 601.0 0 63 279 87 601.0 0 87 280 78 601.0 0 78 281 64 601.0 0 64 282 49 601.0 0 49 283 62 601.0 0 62 284 70 601.0 0 70 285 68 601.0 0 68 286 47 601.0 0 47 287 47 601.0 0 47 288 58 601.0 0 58 289 44 601.0 0 44 290 61 601.0 0 61 291 57 601.0 0 57 292 70 601.0 0 70 293 76 601.0 0 76 294 110 601.0 0 110 295 284 601.0 0 284 296 359 601.0 0 359 297 423 601.0 0 423 298 141 601.0 0 141 299 59 601.0 0 59 300 43 601.0 0 43 301 54 601.0 0 54 Finished in 6.86 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 148,619 Reads with adapters: 64,881 (43.7%) Reads that were too short: 520 (0.3%) Reads written (passing filters): 64,361 (43.3%) Total basepairs processed: 41,983,718 bp Total written (filtered): 17,819,222 bp (42.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 64881 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 360 0.0 3 0 346 13 1 17 63733 0.0 3 62394 1306 30 3 18 147 0.0 3 19 123 5 19 23 0.0 3 0 0 18 5 20 39 0.0 3 0 0 0 39 21 1 0.0 3 0 0 1 23 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 43 19 0.0 3 0 0 0 19 44 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 49 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 1 1 97 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 117 1 0.0 3 1 119 1 0.0 3 0 0 1 120 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 150 1 0.0 3 1 163 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 5 0.0 3 0 0 0 5 217 476 0.0 3 0 0 9 467 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 235 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 288 1 0.0 3 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.28 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 64,361 Reads with adapters: 340 (0.5%) Reads written (passing filters): 64,361 (100.0%) Total basepairs processed: 17,819,222 bp Total written (filtered): 17,818,188 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 340 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.2% C: 18.2% G: 10.9% T: 19.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 333 1005.6 0 333 4 3 251.4 0 3 5 1 62.9 0 1 6 3 15.7 0 3