This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.63 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 140,773 (5.7%) Reads that were too short: 58,594 (2.4%) Reads written (passing filters): 82,179 (3.3%) Total basepairs processed: 740,966,583 bp Total written (filtered): 21,338,723 bp (2.9%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 140773 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2348 601.0 0 2348 7 26563 601.0 0 26563 8 2092 601.0 0 2092 9 30700 601.0 0 30700 10 54 601.0 0 54 11 34 601.0 0 34 12 25 601.0 0 25 13 22 601.0 0 22 14 55 601.0 0 55 15 40 601.0 0 40 16 76 601.0 0 76 17 64 601.0 0 64 18 79 601.0 0 79 19 25 601.0 0 25 20 22 601.0 0 22 21 25 601.0 0 25 22 32 601.0 0 32 23 29 601.0 0 29 24 27 601.0 0 27 25 58 601.0 0 58 26 28 601.0 0 28 27 29 601.0 0 29 28 27 601.0 0 27 29 35 601.0 0 35 30 30 601.0 0 30 31 26 601.0 0 26 32 36 601.0 0 36 33 33 601.0 0 33 34 34 601.0 0 34 35 38 601.0 0 38 36 33 601.0 0 33 37 21 601.0 0 21 38 26 601.0 0 26 39 18 601.0 0 18 40 19 601.0 0 19 41 32 601.0 0 32 42 20 601.0 0 20 43 27 601.0 0 27 44 35 601.0 0 35 45 32 601.0 0 32 46 34 601.0 0 34 47 32 601.0 0 32 48 42 601.0 0 42 49 36 601.0 0 36 50 36 601.0 0 36 51 43 601.0 0 43 52 37 601.0 0 37 53 37 601.0 0 37 54 35 601.0 0 35 55 41 601.0 0 41 56 26 601.0 0 26 57 36 601.0 0 36 58 28 601.0 0 28 59 44 601.0 0 44 60 37 601.0 0 37 61 81 601.0 0 81 62 45 601.0 0 45 63 81 601.0 0 81 64 32 601.0 0 32 65 30 601.0 0 30 66 59 601.0 0 59 67 815 601.0 0 815 68 390 601.0 0 390 69 182 601.0 0 182 70 44 601.0 0 44 71 39 601.0 0 39 72 56 601.0 0 56 73 32 601.0 0 32 74 36 601.0 0 36 75 34 601.0 0 34 76 36 601.0 0 36 77 28 601.0 0 28 78 36 601.0 0 36 79 24 601.0 0 24 80 36 601.0 0 36 81 50 601.0 0 50 82 50 601.0 0 50 83 37 601.0 0 37 84 52 601.0 0 52 85 73 601.0 0 73 86 86 601.0 0 86 87 52 601.0 0 52 88 25 601.0 0 25 89 34 601.0 0 34 90 36 601.0 0 36 91 32 601.0 0 32 92 48 601.0 0 48 93 40 601.0 0 40 94 93 601.0 0 93 95 36 601.0 0 36 96 68 601.0 0 68 97 24 601.0 0 24 98 52 601.0 0 52 99 49 601.0 0 49 100 58 601.0 0 58 101 27 601.0 0 27 102 31 601.0 0 31 103 35 601.0 0 35 104 31 601.0 0 31 105 33 601.0 0 33 106 47 601.0 0 47 107 76 601.0 0 76 108 23 601.0 0 23 109 35 601.0 0 35 110 25 601.0 0 25 111 36 601.0 0 36 112 30 601.0 0 30 113 36 601.0 0 36 114 36 601.0 0 36 115 31 601.0 0 31 116 23 601.0 0 23 117 34 601.0 0 34 118 117 601.0 0 117 119 89 601.0 0 89 120 96 601.0 0 96 121 97 601.0 0 97 122 85 601.0 0 85 123 60 601.0 0 60 124 28 601.0 0 28 125 39 601.0 0 39 126 84 601.0 0 84 127 75 601.0 0 75 128 71 601.0 0 71 129 105 601.0 0 105 130 75 601.0 0 75 131 99 601.0 0 99 132 382 601.0 0 382 133 149 601.0 0 149 134 121 601.0 0 121 135 43 601.0 0 43 136 37 601.0 0 37 137 42 601.0 0 42 138 34 601.0 0 34 139 58 601.0 0 58 140 45 601.0 0 45 141 52 601.0 0 52 142 24 601.0 0 24 143 37 601.0 0 37 144 56 601.0 0 56 145 65 601.0 0 65 146 55 601.0 0 55 147 87 601.0 0 87 148 78 601.0 0 78 149 69 601.0 0 69 150 65 601.0 0 65 151 70 601.0 0 70 152 276 601.0 0 276 153 470 601.0 0 470 154 327 601.0 0 327 155 99 601.0 0 99 156 54 601.0 0 54 157 55 601.0 0 55 158 198 601.0 0 198 159 145 601.0 0 145 160 130 601.0 0 130 161 85 601.0 0 85 162 95 601.0 0 95 163 789 601.0 0 789 164 772 601.0 0 772 165 480 601.0 0 480 166 192 601.0 0 192 167 124 601.0 0 124 168 86 601.0 0 86 169 140 601.0 0 140 170 60 601.0 0 60 171 56 601.0 0 56 172 62 601.0 0 62 173 115 601.0 0 115 174 120 601.0 0 120 175 113 601.0 0 113 176 83 601.0 0 83 177 79 601.0 0 79 178 168 601.0 0 168 179 190 601.0 0 190 180 172 601.0 0 172 181 121 601.0 0 121 182 106 601.0 0 106 183 843 601.0 0 843 184 711 601.0 0 711 185 705 601.0 0 705 186 349 601.0 0 349 187 123 601.0 0 123 188 79 601.0 0 79 189 80 601.0 0 80 190 71 601.0 0 71 191 130 601.0 0 130 192 266 601.0 0 266 193 640 601.0 0 640 194 706 601.0 0 706 195 625 601.0 0 625 196 88 601.0 0 88 197 67 601.0 0 67 198 200 601.0 0 200 199 238 601.0 0 238 200 214 601.0 0 214 201 200 601.0 0 200 202 263 601.0 0 263 203 121 601.0 0 121 204 171 601.0 0 171 205 286 601.0 0 286 206 283 601.0 0 283 207 188 601.0 0 188 208 122 601.0 0 122 209 121 601.0 0 121 210 93 601.0 0 93 211 59 601.0 0 59 212 115 601.0 0 115 213 114 601.0 0 114 214 216 601.0 0 216 215 336 601.0 0 336 216 386 601.0 0 386 217 540 601.0 0 540 218 846 601.0 0 846 219 9004 601.0 0 9004 220 7314 601.0 0 7314 221 22193 601.0 0 22193 222 343 601.0 0 343 223 74 601.0 0 74 224 83 601.0 0 83 225 59 601.0 0 59 226 68 601.0 0 68 227 48 601.0 0 48 228 61 601.0 0 61 229 129 601.0 0 129 230 83 601.0 0 83 231 71 601.0 0 71 232 79 601.0 0 79 233 106 601.0 0 106 234 108 601.0 0 108 235 123 601.0 0 123 236 149 601.0 0 149 237 188 601.0 0 188 238 172 601.0 0 172 239 161 601.0 0 161 240 163 601.0 0 163 241 98 601.0 0 98 242 82 601.0 0 82 243 63 601.0 0 63 244 82 601.0 0 82 245 76 601.0 0 76 246 125 601.0 0 125 247 191 601.0 0 191 248 546 601.0 0 546 249 933 601.0 0 933 250 617 601.0 0 617 251 324 601.0 0 324 252 324 601.0 0 324 253 277 601.0 0 277 254 199 601.0 0 199 255 122 601.0 0 122 256 145 601.0 0 145 257 108 601.0 0 108 258 95 601.0 0 95 259 86 601.0 0 86 260 84 601.0 0 84 261 73 601.0 0 73 262 68 601.0 0 68 263 71 601.0 0 71 264 67 601.0 0 67 265 92 601.0 0 92 266 102 601.0 0 102 267 168 601.0 0 168 268 125 601.0 0 125 269 124 601.0 0 124 270 94 601.0 0 94 271 122 601.0 0 122 272 281 601.0 0 281 273 327 601.0 0 327 274 214 601.0 0 214 275 96 601.0 0 96 276 70 601.0 0 70 277 108 601.0 0 108 278 144 601.0 0 144 279 613 601.0 0 613 280 986 601.0 0 986 281 1121 601.0 0 1121 282 408 601.0 0 408 283 180 601.0 0 180 284 232 601.0 0 232 285 132 601.0 0 132 286 102 601.0 0 102 287 147 601.0 0 147 288 142 601.0 0 142 289 107 601.0 0 107 290 90 601.0 0 90 291 106 601.0 0 106 292 236 601.0 0 236 293 229 601.0 0 229 294 255 601.0 0 255 295 178 601.0 0 178 296 145 601.0 0 145 297 163 601.0 0 163 298 132 601.0 0 132 299 249 601.0 0 249 300 412 601.0 0 412 301 267 601.0 0 267 Finished in 4.36 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 82,179 Reads with adapters: 29,533 (35.9%) Reads that were too short: 61 (0.1%) Reads written (passing filters): 29,472 (35.9%) Total basepairs processed: 21,338,723 bp Total written (filtered): 7,963,323 bp (37.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29533 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 1 3 18 15 0.0 3 0 0 8 5 2 19 447 0.0 3 0 373 35 27 12 20 27797 0.0 4 25451 1843 383 93 27 21 65 0.0 4 9 47 4 4 1 22 16 0.0 4 9 0 2 2 3 23 78 0.0 4 0 0 0 49 29 29 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 44 1 0.0 4 0 0 0 1 57 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 103 3 0.0 4 0 0 0 0 3 107 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 2 0.0 4 0 0 0 0 2 124 2 0.0 4 0 0 0 0 2 126 7 0.0 4 0 0 0 0 7 127 1023 0.0 4 0 0 0 0 1023 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 1 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 17 0.0 4 0 0 0 0 17 229 3 0.0 4 0 0 0 0 3 230 2 0.0 4 0 0 0 1 1 233 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 288 5 0.0 4 0 0 0 0 5 291 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.00 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 29,472 Reads with adapters: 1,524 (5.2%) Reads written (passing filters): 29,472 (100.0%) Total basepairs processed: 7,963,323 bp Total written (filtered): 7,945,433 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1524 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.4% C: 14.2% G: 73.4% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 169 460.5 0 169 4 31 115.1 0 31 7 1 1.8 0 1 9 12 0.1 0 1 11 10 27 0.0 1 0 27 11 587 0.0 1 20 567 12 668 0.0 1 11 657 13 16 0.0 1 0 16 15 2 0.0 1 0 2 122 1 0.0 1 0 1 152 2 0.0 1 2 153 1 0.0 1 1 210 1 0.0 1 1 211 1 0.0 1 1 225 3 0.0 1 1 2 226 1 0.0 1 1 262 1 0.0 1 1