This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.46 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 127,295 (5.2%) Reads that were too short: 49,146 (2.0%) Reads written (passing filters): 78,149 (3.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 21,526,912 bp (2.9%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 127295 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1293 601.0 0 1293 7 34481 601.0 0 34481 8 1160 601.0 0 1160 9 28801 601.0 0 28801 10 70 601.0 0 70 11 46 601.0 0 46 12 33 601.0 0 33 13 27 601.0 0 27 14 48 601.0 0 48 15 34 601.0 0 34 16 39 601.0 0 39 17 36 601.0 0 36 18 33 601.0 0 33 19 32 601.0 0 32 20 38 601.0 0 38 21 42 601.0 0 42 22 48 601.0 0 48 23 42 601.0 0 42 24 33 601.0 0 33 25 74 601.0 0 74 26 45 601.0 0 45 27 26 601.0 0 26 28 42 601.0 0 42 29 45 601.0 0 45 30 38 601.0 0 38 31 43 601.0 0 43 32 42 601.0 0 42 33 33 601.0 0 33 34 35 601.0 0 35 35 54 601.0 0 54 36 48 601.0 0 48 37 35 601.0 0 35 38 30 601.0 0 30 39 36 601.0 0 36 40 43 601.0 0 43 41 34 601.0 0 34 42 26 601.0 0 26 43 42 601.0 0 42 44 41 601.0 0 41 45 42 601.0 0 42 46 40 601.0 0 40 47 43 601.0 0 43 48 37 601.0 0 37 49 50 601.0 0 50 50 35 601.0 0 35 51 44 601.0 0 44 52 56 601.0 0 56 53 56 601.0 0 56 54 43 601.0 0 43 55 43 601.0 0 43 56 38 601.0 0 38 57 49 601.0 0 49 58 44 601.0 0 44 59 51 601.0 0 51 60 41 601.0 0 41 61 73 601.0 0 73 62 60 601.0 0 60 63 71 601.0 0 71 64 48 601.0 0 48 65 42 601.0 0 42 66 48 601.0 0 48 67 459 601.0 0 459 68 274 601.0 0 274 69 116 601.0 0 116 70 33 601.0 0 33 71 60 601.0 0 60 72 54 601.0 0 54 73 38 601.0 0 38 74 45 601.0 0 45 75 57 601.0 0 57 76 55 601.0 0 55 77 35 601.0 0 35 78 55 601.0 0 55 79 40 601.0 0 40 80 42 601.0 0 42 81 53 601.0 0 53 82 46 601.0 0 46 83 44 601.0 0 44 84 39 601.0 0 39 85 36 601.0 0 36 86 70 601.0 0 70 87 40 601.0 0 40 88 27 601.0 0 27 89 56 601.0 0 56 90 42 601.0 0 42 91 33 601.0 0 33 92 47 601.0 0 47 93 53 601.0 0 53 94 63 601.0 0 63 95 44 601.0 0 44 96 53 601.0 0 53 97 55 601.0 0 55 98 50 601.0 0 50 99 68 601.0 0 68 100 42 601.0 0 42 101 40 601.0 0 40 102 46 601.0 0 46 103 21 601.0 0 21 104 41 601.0 0 41 105 47 601.0 0 47 106 43 601.0 0 43 107 92 601.0 0 92 108 34 601.0 0 34 109 30 601.0 0 30 110 35 601.0 0 35 111 49 601.0 0 49 112 42 601.0 0 42 113 63 601.0 0 63 114 34 601.0 0 34 115 33 601.0 0 33 116 46 601.0 0 46 117 48 601.0 0 48 118 62 601.0 0 62 119 50 601.0 0 50 120 72 601.0 0 72 121 51 601.0 0 51 122 74 601.0 0 74 123 56 601.0 0 56 124 37 601.0 0 37 125 41 601.0 0 41 126 74 601.0 0 74 127 50 601.0 0 50 128 54 601.0 0 54 129 113 601.0 0 113 130 122 601.0 0 122 131 102 601.0 0 102 132 397 601.0 0 397 133 105 601.0 0 105 134 136 601.0 0 136 135 40 601.0 0 40 136 41 601.0 0 41 137 39 601.0 0 39 138 51 601.0 0 51 139 47 601.0 0 47 140 45 601.0 0 45 141 47 601.0 0 47 142 37 601.0 0 37 143 42 601.0 0 42 144 52 601.0 0 52 145 45 601.0 0 45 146 43 601.0 0 43 147 52 601.0 0 52 148 47 601.0 0 47 149 42 601.0 0 42 150 49 601.0 0 49 151 46 601.0 0 46 152 130 601.0 0 130 153 159 601.0 0 159 154 98 601.0 0 98 155 61 601.0 0 61 156 51 601.0 0 51 157 37 601.0 0 37 158 136 601.0 0 136 159 108 601.0 0 108 160 84 601.0 0 84 161 61 601.0 0 61 162 55 601.0 0 55 163 148 601.0 0 148 164 128 601.0 0 128 165 79 601.0 0 79 166 61 601.0 0 61 167 64 601.0 0 64 168 58 601.0 0 58 169 66 601.0 0 66 170 45 601.0 0 45 171 48 601.0 0 48 172 58 601.0 0 58 173 69 601.0 0 69 174 59 601.0 0 59 175 47 601.0 0 47 176 48 601.0 0 48 177 58 601.0 0 58 178 67 601.0 0 67 179 71 601.0 0 71 180 73 601.0 0 73 181 63 601.0 0 63 182 48 601.0 0 48 183 168 601.0 0 168 184 178 601.0 0 178 185 133 601.0 0 133 186 212 601.0 0 212 187 48 601.0 0 48 188 36 601.0 0 36 189 64 601.0 0 64 190 57 601.0 0 57 191 65 601.0 0 65 192 149 601.0 0 149 193 248 601.0 0 248 194 185 601.0 0 185 195 172 601.0 0 172 196 49 601.0 0 49 197 50 601.0 0 50 198 69 601.0 0 69 199 106 601.0 0 106 200 74 601.0 0 74 201 117 601.0 0 117 202 163 601.0 0 163 203 64 601.0 0 64 204 104 601.0 0 104 205 122 601.0 0 122 206 120 601.0 0 120 207 87 601.0 0 87 208 72 601.0 0 72 209 76 601.0 0 76 210 65 601.0 0 65 211 50 601.0 0 50 212 125 601.0 0 125 213 110 601.0 0 110 214 174 601.0 0 174 215 277 601.0 0 277 216 326 601.0 0 326 217 442 601.0 0 442 218 609 601.0 0 609 219 7753 601.0 0 7753 220 7424 601.0 0 7424 221 23652 601.0 0 23652 222 345 601.0 0 345 223 48 601.0 0 48 224 42 601.0 0 42 225 48 601.0 0 48 226 43 601.0 0 43 227 50 601.0 0 50 228 53 601.0 0 53 229 58 601.0 0 58 230 40 601.0 0 40 231 41 601.0 0 41 232 43 601.0 0 43 233 36 601.0 0 36 234 64 601.0 0 64 235 67 601.0 0 67 236 58 601.0 0 58 237 78 601.0 0 78 238 63 601.0 0 63 239 65 601.0 0 65 240 74 601.0 0 74 241 89 601.0 0 89 242 46 601.0 0 46 243 55 601.0 0 55 244 59 601.0 0 59 245 46 601.0 0 46 246 74 601.0 0 74 247 79 601.0 0 79 248 316 601.0 0 316 249 454 601.0 0 454 250 274 601.0 0 274 251 120 601.0 0 120 252 99 601.0 0 99 253 73 601.0 0 73 254 77 601.0 0 77 255 82 601.0 0 82 256 62 601.0 0 62 257 48 601.0 0 48 258 49 601.0 0 49 259 40 601.0 0 40 260 52 601.0 0 52 261 40 601.0 0 40 262 49 601.0 0 49 263 44 601.0 0 44 264 54 601.0 0 54 265 39 601.0 0 39 266 47 601.0 0 47 267 63 601.0 0 63 268 55 601.0 0 55 269 71 601.0 0 71 270 61 601.0 0 61 271 64 601.0 0 64 272 99 601.0 0 99 273 121 601.0 0 121 274 90 601.0 0 90 275 68 601.0 0 68 276 38 601.0 0 38 277 63 601.0 0 63 278 78 601.0 0 78 279 269 601.0 0 269 280 417 601.0 0 417 281 326 601.0 0 326 282 165 601.0 0 165 283 54 601.0 0 54 284 74 601.0 0 74 285 61 601.0 0 61 286 87 601.0 0 87 287 72 601.0 0 72 288 62 601.0 0 62 289 73 601.0 0 73 290 64 601.0 0 64 291 57 601.0 0 57 292 98 601.0 0 98 293 78 601.0 0 78 294 89 601.0 0 89 295 75 601.0 0 75 296 52 601.0 0 52 297 64 601.0 0 64 298 62 601.0 0 62 299 96 601.0 0 96 300 141 601.0 0 141 301 141 601.0 0 141 Finished in 3.89 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 78,149 Reads with adapters: 30,472 (39.0%) Reads that were too short: 385 (0.5%) Reads written (passing filters): 30,087 (38.5%) Total basepairs processed: 21,526,912 bp Total written (filtered): 8,271,661 bp (38.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30472 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 191 0.0 3 0 185 6 17 29777 0.0 3 29222 540 14 1 18 56 0.0 3 1 54 0 1 19 19 0.0 3 0 0 19 20 11 0.0 3 1 0 0 10 23 1 0.0 3 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 79 1 0.0 3 0 1 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 1 107 1 0.0 3 0 0 0 1 109 38 0.0 3 0 0 0 38 110 2 0.0 3 0 0 0 2 116 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 173 2 0.0 3 2 179 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 216 6 0.0 3 0 0 0 6 217 332 0.0 3 0 0 6 326 218 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.06 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 30,087 Reads with adapters: 357 (1.2%) Reads written (passing filters): 30,087 (100.0%) Total basepairs processed: 8,271,661 bp Total written (filtered): 8,270,540 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 357 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.3% C: 3.9% G: 39.5% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 308 470.1 0 308 4 48 117.5 0 48 5 1 29.4 0 1