This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.90 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 529,037 (21.5%) Reads that were too short: 34,190 (1.4%) Reads written (passing filters): 494,847 (20.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 115,196,386 bp (15.5%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 529037 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1162 601.0 0 1162 7 4071 601.0 0 4071 8 93422 601.0 0 93422 9 75654 601.0 0 75654 10 155 601.0 0 155 11 545 601.0 0 545 12 482 601.0 0 482 13 35 601.0 0 35 14 31 601.0 0 31 15 35 601.0 0 35 16 45 601.0 0 45 17 44 601.0 0 44 18 27 601.0 0 27 19 30 601.0 0 30 20 26 601.0 0 26 21 39 601.0 0 39 22 33 601.0 0 33 23 53 601.0 0 53 24 51 601.0 0 51 25 43 601.0 0 43 26 60 601.0 0 60 27 52 601.0 0 52 28 48 601.0 0 48 29 37 601.0 0 37 30 51 601.0 0 51 31 51 601.0 0 51 32 73 601.0 0 73 33 33 601.0 0 33 34 33 601.0 0 33 35 31 601.0 0 31 36 40 601.0 0 40 37 27 601.0 0 27 38 71 601.0 0 71 39 319 601.0 0 319 40 260 601.0 0 260 41 129 601.0 0 129 42 58 601.0 0 58 43 59 601.0 0 59 44 25 601.0 0 25 45 39 601.0 0 39 46 25 601.0 0 25 47 53 601.0 0 53 48 95 601.0 0 95 49 88 601.0 0 88 50 66 601.0 0 66 51 71 601.0 0 71 52 106 601.0 0 106 53 124 601.0 0 124 54 160 601.0 0 160 55 36 601.0 0 36 56 31 601.0 0 31 57 72 601.0 0 72 58 60 601.0 0 60 59 37 601.0 0 37 60 70 601.0 0 70 61 49 601.0 0 49 62 62 601.0 0 62 63 83 601.0 0 83 64 36 601.0 0 36 65 65 601.0 0 65 66 99 601.0 0 99 67 74 601.0 0 74 68 232 601.0 0 232 69 91 601.0 0 91 70 64 601.0 0 64 71 68 601.0 0 68 72 170 601.0 0 170 73 129 601.0 0 129 74 152 601.0 0 152 75 167 601.0 0 167 76 274 601.0 0 274 77 421 601.0 0 421 78 799 601.0 0 799 79 1468 601.0 0 1468 80 435 601.0 0 435 81 560 601.0 0 560 82 967 601.0 0 967 83 492 601.0 0 492 84 405 601.0 0 405 85 811 601.0 0 811 86 965 601.0 0 965 87 856 601.0 0 856 88 1084 601.0 0 1084 89 1702 601.0 0 1702 90 2650 601.0 0 2650 91 5251 601.0 0 5251 92 145670 601.0 0 145670 93 56804 601.0 0 56804 94 62532 601.0 0 62532 95 274 601.0 0 274 96 28 601.0 0 28 97 67 601.0 0 67 98 121 601.0 0 121 99 41 601.0 0 41 100 31 601.0 0 31 101 29 601.0 0 29 102 38 601.0 0 38 103 16 601.0 0 16 104 30 601.0 0 30 105 16 601.0 0 16 106 24 601.0 0 24 107 18 601.0 0 18 108 42 601.0 0 42 109 42 601.0 0 42 110 165 601.0 0 165 111 75 601.0 0 75 112 85 601.0 0 85 113 25 601.0 0 25 114 80 601.0 0 80 115 99 601.0 0 99 116 68 601.0 0 68 117 70 601.0 0 70 118 46 601.0 0 46 119 43 601.0 0 43 120 13 601.0 0 13 121 14 601.0 0 14 122 15 601.0 0 15 123 22 601.0 0 22 124 29 601.0 0 29 125 46 601.0 0 46 126 27 601.0 0 27 127 24 601.0 0 24 128 36 601.0 0 36 129 63 601.0 0 63 130 65 601.0 0 65 131 210 601.0 0 210 132 46 601.0 0 46 133 29 601.0 0 29 134 26 601.0 0 26 135 28 601.0 0 28 136 48 601.0 0 48 137 54 601.0 0 54 138 67 601.0 0 67 139 53 601.0 0 53 140 52 601.0 0 52 141 32 601.0 0 32 142 31 601.0 0 31 143 30 601.0 0 30 144 41 601.0 0 41 145 28 601.0 0 28 146 53 601.0 0 53 147 17 601.0 0 17 148 35 601.0 0 35 149 34 601.0 0 34 150 28 601.0 0 28 151 42 601.0 0 42 152 30 601.0 0 30 153 23 601.0 0 23 154 25 601.0 0 25 155 47 601.0 0 47 156 29 601.0 0 29 157 51 601.0 0 51 158 50 601.0 0 50 159 38 601.0 0 38 160 19 601.0 0 19 161 26 601.0 0 26 162 23 601.0 0 23 163 20 601.0 0 20 164 27 601.0 0 27 165 26 601.0 0 26 166 21 601.0 0 21 167 28 601.0 0 28 168 22 601.0 0 22 169 35 601.0 0 35 170 28 601.0 0 28 171 44 601.0 0 44 172 28 601.0 0 28 173 21 601.0 0 21 174 29 601.0 0 29 175 38 601.0 0 38 176 36 601.0 0 36 177 86 601.0 0 86 178 224 601.0 0 224 179 261 601.0 0 261 180 257 601.0 0 257 181 86 601.0 0 86 182 38 601.0 0 38 183 80 601.0 0 80 184 59 601.0 0 59 185 68 601.0 0 68 186 158 601.0 0 158 187 369 601.0 0 369 188 508 601.0 0 508 189 128 601.0 0 128 190 183 601.0 0 183 191 328 601.0 0 328 192 136 601.0 0 136 193 127 601.0 0 127 194 214 601.0 0 214 195 184 601.0 0 184 196 174 601.0 0 174 197 221 601.0 0 221 198 368 601.0 0 368 199 437 601.0 0 437 200 930 601.0 0 930 201 21288 601.0 0 21288 202 6762 601.0 0 6762 203 9027 601.0 0 9027 204 440 601.0 0 440 205 418 601.0 0 418 206 1258 601.0 0 1258 207 1117 601.0 0 1117 208 353 601.0 0 353 209 143 601.0 0 143 210 79 601.0 0 79 211 115 601.0 0 115 212 201 601.0 0 201 213 295 601.0 0 295 214 77 601.0 0 77 215 66 601.0 0 66 216 203 601.0 0 203 217 128 601.0 0 128 218 84 601.0 0 84 219 91 601.0 0 91 220 61 601.0 0 61 221 169 601.0 0 169 222 162 601.0 0 162 223 201 601.0 0 201 224 91 601.0 0 91 225 507 601.0 0 507 226 936 601.0 0 936 227 1379 601.0 0 1379 228 531 601.0 0 531 229 526 601.0 0 526 230 46 601.0 0 46 231 26 601.0 0 26 232 29 601.0 0 29 233 39 601.0 0 39 234 33 601.0 0 33 235 26 601.0 0 26 236 87 601.0 0 87 237 78 601.0 0 78 238 67 601.0 0 67 239 25 601.0 0 25 240 11 601.0 0 11 241 30 601.0 0 30 242 40 601.0 0 40 243 30 601.0 0 30 244 34 601.0 0 34 245 55 601.0 0 55 246 51 601.0 0 51 247 60 601.0 0 60 248 50 601.0 0 50 249 45 601.0 0 45 250 63 601.0 0 63 251 49 601.0 0 49 252 29 601.0 0 29 253 43 601.0 0 43 254 39 601.0 0 39 255 42 601.0 0 42 256 46 601.0 0 46 257 62 601.0 0 62 258 66 601.0 0 66 259 252 601.0 0 252 260 607 601.0 0 607 261 913 601.0 0 913 262 679 601.0 0 679 263 251 601.0 0 251 264 270 601.0 0 270 265 66 601.0 0 66 266 39 601.0 0 39 267 38 601.0 0 38 268 29 601.0 0 29 269 47 601.0 0 47 270 266 601.0 0 266 271 171 601.0 0 171 272 242 601.0 0 242 273 87 601.0 0 87 274 34 601.0 0 34 275 51 601.0 0 51 276 156 601.0 0 156 277 92 601.0 0 92 278 75 601.0 0 75 279 54 601.0 0 54 280 122 601.0 0 122 281 122 601.0 0 122 282 158 601.0 0 158 283 84 601.0 0 84 284 121 601.0 0 121 285 109 601.0 0 109 286 233 601.0 0 233 287 66 601.0 0 66 288 86 601.0 0 86 289 38 601.0 0 38 290 36 601.0 0 36 291 27 601.0 0 27 292 43 601.0 0 43 293 70 601.0 0 70 294 56 601.0 0 56 295 71 601.0 0 71 296 112 601.0 0 112 297 850 601.0 0 850 298 224 601.0 0 224 299 368 601.0 0 368 300 34 601.0 0 34 301 20 601.0 0 20 Finished in 22.39 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 494,847 Reads with adapters: 81,336 (16.4%) Reads that were too short: 218 (0.0%) Reads written (passing filters): 81,118 (16.4%) Total basepairs processed: 115,196,386 bp Total written (filtered): 21,992,535 bp (19.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 81336 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 13 0.0 3 0 0 0 7 6 18 73 0.0 3 0 0 43 14 16 19 1500 0.0 3 0 1326 89 60 25 20 78360 0.0 4 72645 4448 942 245 80 21 169 0.0 4 18 126 11 9 5 22 14 0.0 4 0 0 10 1 3 23 266 0.0 4 4 3 0 179 80 24 4 0.0 4 1 0 1 1 1 32 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 43 3 0.0 4 0 0 0 0 3 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 63 4 0.0 4 0 0 0 0 4 66 2 0.0 4 0 0 0 0 2 67 10 0.0 4 0 0 0 0 10 68 66 0.0 4 0 0 0 2 64 69 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 7 0.0 4 0 0 0 0 7 93 1 0.0 4 1 94 4 0.0 4 0 0 0 0 4 95 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 106 4 0.0 4 0 0 0 0 4 107 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 12 0.0 4 0 1 0 0 11 123 3 0.0 4 0 0 0 0 3 125 2 0.0 4 0 0 0 0 2 126 6 0.0 4 0 0 0 1 5 127 596 0.0 4 0 0 0 0 596 128 2 0.0 4 0 0 0 0 2 129 2 0.0 4 1 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 135 8 0.0 4 0 0 0 0 8 137 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 141 3 0.0 4 1 0 0 0 2 142 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 11 0.0 4 0 0 0 0 11 149 20 0.0 4 0 0 0 1 19 150 14 0.0 4 0 0 0 2 12 151 11 0.0 4 0 0 0 1 10 153 1 0.0 4 0 0 0 0 1 169 2 0.0 4 0 0 0 0 2 171 3 0.0 4 0 0 0 0 3 172 4 0.0 4 0 0 0 0 4 173 3 0.0 4 0 0 0 0 3 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 179 2 0.0 4 0 0 0 0 2 185 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 197 2 0.0 4 0 0 0 0 2 198 7 0.0 4 0 0 0 0 7 199 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 207 3 0.0 4 0 0 0 0 3 215 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 1 221 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 228 18 0.0 4 0 0 0 2 16 229 7 0.0 4 0 0 0 0 7 230 4 0.0 4 0 0 0 0 4 231 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 233 4 0.0 4 0 0 0 0 4 234 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 236 3 0.0 4 0 0 0 0 3 237 3 0.0 4 0 0 0 0 3 253 1 0.0 4 0 0 0 0 1 254 4 0.0 4 0 0 0 0 4 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 264 2 0.0 4 0 0 0 0 2 278 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 288 5 0.0 4 0 0 0 0 5 291 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.98 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 81,118 Reads with adapters: 19,002 (23.4%) Reads written (passing filters): 81,118 (100.0%) Total basepairs processed: 21,992,535 bp Total written (filtered): 21,800,675 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 19002 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 8.9% G: 87.0% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 34 1267.5 0 34 4 25 316.9 0 25 5 3 79.2 0 3 6 4 19.8 0 4 7 7 5.0 0 7 8 4 1.2 0 4 9 3168 0.3 0 292 2876 10 13717 0.1 1 925 12792 11 1907 0.0 1 64 1843 12 56 0.0 1 3 53 13 54 0.0 1 0 54 14 1 0.0 1 0 1 15 1 0.0 1 0 1 25 1 0.0 1 0 1 36 2 0.0 1 1 1 150 7 0.0 1 4 3 152 1 0.0 1 1 161 1 0.0 1 0 1 190 1 0.0 1 0 1 208 4 0.0 1 4 228 1 0.0 1 0 1 251 1 0.0 1 1 257 1 0.0 1 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.