This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.67 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 345,202 (14.1%) Reads that were too short: 7,929 (0.3%) Reads written (passing filters): 337,273 (13.8%) Total basepairs processed: 735,190,694 bp Total written (filtered): 75,604,765 bp (10.3%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 345202 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 172 596.3 0 172 7 11979 596.3 0 11979 8 9926 596.3 0 9926 9 20 596.3 0 20 10 46 596.3 0 46 11 11 596.3 0 11 12 13 596.3 0 13 13 14 596.3 0 14 14 8 596.3 0 8 15 6 596.3 0 6 16 10 596.3 0 10 17 5 596.3 0 5 18 12 596.3 0 12 19 21 596.3 0 21 20 18 596.3 0 18 21 45 596.3 0 45 22 35 596.3 0 35 23 24 596.3 0 24 24 5 596.3 0 5 25 6 596.3 0 6 26 13 596.3 0 13 27 28 596.3 0 28 28 11 596.3 0 11 29 20 596.3 0 20 30 18 596.3 0 18 31 16 596.3 0 16 32 12 596.3 0 12 33 34 596.3 0 34 34 17 596.3 0 17 35 57 596.3 0 57 36 16 596.3 0 16 37 10 596.3 0 10 38 10 596.3 0 10 39 32 596.3 0 32 40 35 596.3 0 35 41 26 596.3 0 26 42 108 596.3 0 108 43 67 596.3 0 67 44 77 596.3 0 77 45 60 596.3 0 60 46 57 596.3 0 57 47 46 596.3 0 46 48 28 596.3 0 28 49 137 596.3 0 137 50 38 596.3 0 38 51 88 596.3 0 88 52 781 596.3 0 781 53 97 596.3 0 97 54 94 596.3 0 94 55 206 596.3 0 206 56 19 596.3 0 19 57 25 596.3 0 25 58 17 596.3 0 17 59 102 596.3 0 102 60 78 596.3 0 78 61 299 596.3 0 299 62 980 596.3 0 980 63 131 596.3 0 131 64 315 596.3 0 315 65 400 596.3 0 400 66 890 596.3 0 890 67 1683 596.3 0 1683 68 279 596.3 0 279 69 331 596.3 0 331 70 116 596.3 0 116 71 191 596.3 0 191 72 451 596.3 0 451 73 536 596.3 0 536 74 860 596.3 0 860 75 1365 596.3 0 1365 76 1506 596.3 0 1506 77 3143 596.3 0 3143 78 7356 596.3 0 7356 79 117535 596.3 0 117535 80 48857 596.3 0 48857 81 113115 596.3 0 113115 82 613 596.3 0 613 83 219 596.3 0 219 84 38 596.3 0 38 85 23 596.3 0 23 86 13 596.3 0 13 87 27 596.3 0 27 88 25 596.3 0 25 89 14 596.3 0 14 90 16 596.3 0 16 91 11 596.3 0 11 92 29 596.3 0 29 93 20 596.3 0 20 94 8 596.3 0 8 95 11 596.3 0 11 96 12 596.3 0 12 97 19 596.3 0 19 98 17 596.3 0 17 99 12 596.3 0 12 100 18 596.3 0 18 101 22 596.3 0 22 102 17 596.3 0 17 103 23 596.3 0 23 104 15 596.3 0 15 105 13 596.3 0 13 106 15 596.3 0 15 107 11 596.3 0 11 108 12 596.3 0 12 109 12 596.3 0 12 110 26 596.3 0 26 111 24 596.3 0 24 112 15 596.3 0 15 113 22 596.3 0 22 114 25 596.3 0 25 115 27 596.3 0 27 116 155 596.3 0 155 117 269 596.3 0 269 118 487 596.3 0 487 119 88 596.3 0 88 120 49 596.3 0 49 121 45 596.3 0 45 122 47 596.3 0 47 123 44 596.3 0 44 124 69 596.3 0 69 125 86 596.3 0 86 126 107 596.3 0 107 127 44 596.3 0 44 128 32 596.3 0 32 129 32 596.3 0 32 130 112 596.3 0 112 131 204 596.3 0 204 132 269 596.3 0 269 133 47 596.3 0 47 134 35 596.3 0 35 135 42 596.3 0 42 136 54 596.3 0 54 137 60 596.3 0 60 138 48 596.3 0 48 139 59 596.3 0 59 140 209 596.3 0 209 141 515 596.3 0 515 142 119 596.3 0 119 143 67 596.3 0 67 144 51 596.3 0 51 145 62 596.3 0 62 146 69 596.3 0 69 147 87 596.3 0 87 148 86 596.3 0 86 149 160 596.3 0 160 150 1696 596.3 0 1696 151 1277 596.3 0 1277 152 1051 596.3 0 1051 153 44 596.3 0 44 154 32 596.3 0 32 155 37 596.3 0 37 156 28 596.3 0 28 157 34 596.3 0 34 158 59 596.3 0 59 159 21 596.3 0 21 160 25 596.3 0 25 161 36 596.3 0 36 162 31 596.3 0 31 163 30 596.3 0 30 164 21 596.3 0 21 165 40 596.3 0 40 166 28 596.3 0 28 167 52 596.3 0 52 168 57 596.3 0 57 169 61 596.3 0 61 170 106 596.3 0 106 171 228 596.3 0 228 172 269 596.3 0 269 173 94 596.3 0 94 174 69 596.3 0 69 175 30 596.3 0 30 176 19 596.3 0 19 177 25 596.3 0 25 178 33 596.3 0 33 179 27 596.3 0 27 180 21 596.3 0 21 181 35 596.3 0 35 182 36 596.3 0 36 183 33 596.3 0 33 184 48 596.3 0 48 185 91 596.3 0 91 186 147 596.3 0 147 187 114 596.3 0 114 188 71 596.3 0 71 189 142 596.3 0 142 190 33 596.3 0 33 191 24 596.3 0 24 192 28 596.3 0 28 193 23 596.3 0 23 194 36 596.3 0 36 195 29 596.3 0 29 196 36 596.3 0 36 197 47 596.3 0 47 198 46 596.3 0 46 199 57 596.3 0 57 200 59 596.3 0 59 201 29 596.3 0 29 202 32 596.3 0 32 203 51 596.3 0 51 204 25 596.3 0 25 205 33 596.3 0 33 206 37 596.3 0 37 207 34 596.3 0 34 208 41 596.3 0 41 209 80 596.3 0 80 210 57 596.3 0 57 211 34 596.3 0 34 212 38 596.3 0 38 213 38 596.3 0 38 214 41 596.3 0 41 215 47 596.3 0 47 216 58 596.3 0 58 217 72 596.3 0 72 218 526 596.3 0 526 219 233 596.3 0 233 220 49 596.3 0 49 221 44 596.3 0 44 222 75 596.3 0 75 223 72 596.3 0 72 224 73 596.3 0 73 225 196 596.3 0 196 226 593 596.3 0 593 227 470 596.3 0 470 228 269 596.3 0 269 229 78 596.3 0 78 230 58 596.3 0 58 231 33 596.3 0 33 232 25 596.3 0 25 233 28 596.3 0 28 234 38 596.3 0 38 235 35 596.3 0 35 236 29 596.3 0 29 237 30 596.3 0 30 238 39 596.3 0 39 239 55 596.3 0 55 240 50 596.3 0 50 241 56 596.3 0 56 242 35 596.3 0 35 243 42 596.3 0 42 244 28 596.3 0 28 245 34 596.3 0 34 246 26 596.3 0 26 247 43 596.3 0 43 248 53 596.3 0 53 249 64 596.3 0 64 250 37 596.3 0 37 251 55 596.3 0 55 252 52 596.3 0 52 253 52 596.3 0 52 254 36 596.3 0 36 255 33 596.3 0 33 256 51 596.3 0 51 257 55 596.3 0 55 258 40 596.3 0 40 259 41 596.3 0 41 260 41 596.3 0 41 261 44 596.3 0 44 262 41 596.3 0 41 263 49 596.3 0 49 264 43 596.3 0 43 265 47 596.3 0 47 266 60 596.3 0 60 267 39 596.3 0 39 268 38 596.3 0 38 269 33 596.3 0 33 270 34 596.3 0 34 271 25 596.3 0 25 272 51 596.3 0 51 273 55 596.3 0 55 274 208 596.3 0 208 275 283 596.3 0 283 276 215 596.3 0 215 277 189 596.3 0 189 278 36 596.3 0 36 279 64 596.3 0 64 280 59 596.3 0 59 281 98 596.3 0 98 282 49 596.3 0 49 283 91 596.3 0 91 284 83 596.3 0 83 285 230 596.3 0 230 286 51 596.3 0 51 287 89 596.3 0 89 288 52 596.3 0 52 289 64 596.3 0 64 290 77 596.3 0 77 291 84 596.3 0 84 292 101 596.3 0 101 293 75 596.3 0 75 294 57 596.3 0 57 295 41 596.3 0 41 296 49 596.3 0 49 297 59 596.3 0 59 298 64 596.3 0 64 299 73 596.3 0 73 300 78 596.3 0 78 301 61 596.3 0 61 Finished in 12.61 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 337,273 Reads with adapters: 9,372 (2.8%) Reads that were too short: 163 (0.0%) Reads written (passing filters): 9,209 (2.7%) Total basepairs processed: 75,604,765 bp Total written (filtered): 2,531,660 bp (3.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9372 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 69 0.0 3 0 66 3 17 8965 0.0 3 8765 194 6 18 29 0.0 3 11 18 19 6 0.0 3 1 0 2 3 20 22 0.0 3 0 0 0 22 35 1 0.0 3 0 0 0 1 41 44 0.0 3 0 0 1 43 44 7 0.0 3 0 0 0 7 56 2 0.0 3 0 1 0 1 57 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 7 0.0 3 0 0 0 7 82 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 12 0.0 3 0 0 0 12 107 3 0.0 3 0 0 0 3 108 3 0.0 3 0 0 0 3 109 4 0.0 3 0 0 0 4 110 4 0.0 3 0 0 1 3 111 4 0.0 3 0 0 1 3 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 1 116 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 128 2 0.0 3 0 0 0 2 133 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 1 144 3 0.0 3 0 0 0 3 145 4 0.0 3 0 0 0 4 146 1 0.0 3 0 0 0 1 149 28 0.0 3 0 0 0 28 150 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 207 17 0.0 3 0 0 0 17 208 20 0.0 3 0 0 1 19 209 19 0.0 3 0 0 0 19 210 26 0.0 3 0 0 0 26 211 3 0.0 3 0 0 0 3 213 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 4 0.0 3 0 0 0 4 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 9,209 Reads with adapters: 93 (1.0%) Reads written (passing filters): 9,209 (100.0%) Total basepairs processed: 2,531,660 bp Total written (filtered): 2,531,372 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 93 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.7% C: 20.4% G: 7.5% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 89 143.9 0 89 4 3 36.0 0 3 9 1 0.0 0 0 1