This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.62 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 91,465 (3.7%) Reads that were too short: 32,638 (1.3%) Reads written (passing filters): 58,827 (2.4%) Total basepairs processed: 735,190,694 bp Total written (filtered): 13,996,564 bp (1.9%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 91465 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 173 596.3 0 173 7 291 596.3 0 291 8 13117 596.3 0 13117 9 14984 596.3 0 14984 10 57 596.3 0 57 11 32 596.3 0 32 12 38 596.3 0 38 13 33 596.3 0 33 14 42 596.3 0 42 15 36 596.3 0 36 16 40 596.3 0 40 17 32 596.3 0 32 18 31 596.3 0 31 19 31 596.3 0 31 20 30 596.3 0 30 21 33 596.3 0 33 22 41 596.3 0 41 23 33 596.3 0 33 24 27 596.3 0 27 25 47 596.3 0 47 26 41 596.3 0 41 27 49 596.3 0 49 28 40 596.3 0 40 29 38 596.3 0 38 30 38 596.3 0 38 31 30 596.3 0 30 32 47 596.3 0 47 33 30 596.3 0 30 34 34 596.3 0 34 35 44 596.3 0 44 36 63 596.3 0 63 37 45 596.3 0 45 38 58 596.3 0 58 39 39 596.3 0 39 40 33 596.3 0 33 41 60 596.3 0 60 42 45 596.3 0 45 43 80 596.3 0 80 44 45 596.3 0 45 45 90 596.3 0 90 46 51 596.3 0 51 47 70 596.3 0 70 48 35 596.3 0 35 49 44 596.3 0 44 50 45 596.3 0 45 51 52 596.3 0 52 52 47 596.3 0 47 53 39 596.3 0 39 54 33 596.3 0 33 55 42 596.3 0 42 56 50 596.3 0 50 57 44 596.3 0 44 58 33 596.3 0 33 59 46 596.3 0 46 60 38 596.3 0 38 61 36 596.3 0 36 62 57 596.3 0 57 63 35 596.3 0 35 64 43 596.3 0 43 65 63 596.3 0 63 66 111 596.3 0 111 67 40 596.3 0 40 68 70 596.3 0 70 69 102 596.3 0 102 70 699 596.3 0 699 71 72 596.3 0 72 72 76 596.3 0 76 73 106 596.3 0 106 74 78 596.3 0 78 75 81 596.3 0 81 76 85 596.3 0 85 77 165 596.3 0 165 78 190 596.3 0 190 79 4329 596.3 0 4329 80 1906 596.3 0 1906 81 2397 596.3 0 2397 82 145 596.3 0 145 83 176 596.3 0 176 84 200 596.3 0 200 85 69 596.3 0 69 86 79 596.3 0 79 87 71 596.3 0 71 88 109 596.3 0 109 89 70 596.3 0 70 90 63 596.3 0 63 91 79 596.3 0 79 92 95 596.3 0 95 93 50 596.3 0 50 94 57 596.3 0 57 95 81 596.3 0 81 96 73 596.3 0 73 97 90 596.3 0 90 98 87 596.3 0 87 99 118 596.3 0 118 100 85 596.3 0 85 101 74 596.3 0 74 102 77 596.3 0 77 103 88 596.3 0 88 104 84 596.3 0 84 105 82 596.3 0 82 106 64 596.3 0 64 107 78 596.3 0 78 108 96 596.3 0 96 109 89 596.3 0 89 110 79 596.3 0 79 111 71 596.3 0 71 112 101 596.3 0 101 113 77 596.3 0 77 114 71 596.3 0 71 115 99 596.3 0 99 116 114 596.3 0 114 117 129 596.3 0 129 118 85 596.3 0 85 119 106 596.3 0 106 120 97 596.3 0 97 121 119 596.3 0 119 122 91 596.3 0 91 123 101 596.3 0 101 124 155 596.3 0 155 125 297 596.3 0 297 126 382 596.3 0 382 127 331 596.3 0 331 128 135 596.3 0 135 129 167 596.3 0 167 130 251 596.3 0 251 131 86 596.3 0 86 132 82 596.3 0 82 133 82 596.3 0 82 134 85 596.3 0 85 135 92 596.3 0 92 136 164 596.3 0 164 137 165 596.3 0 165 138 198 596.3 0 198 139 975 596.3 0 975 140 462 596.3 0 462 141 142 596.3 0 142 142 125 596.3 0 125 143 143 596.3 0 143 144 111 596.3 0 111 145 96 596.3 0 96 146 102 596.3 0 102 147 76 596.3 0 76 148 83 596.3 0 83 149 67 596.3 0 67 150 110 596.3 0 110 151 126 596.3 0 126 152 106 596.3 0 106 153 84 596.3 0 84 154 75 596.3 0 75 155 83 596.3 0 83 156 95 596.3 0 95 157 70 596.3 0 70 158 192 596.3 0 192 159 437 596.3 0 437 160 632 596.3 0 632 161 70 596.3 0 70 162 72 596.3 0 72 163 96 596.3 0 96 164 206 596.3 0 206 165 335 596.3 0 335 166 74 596.3 0 74 167 99 596.3 0 99 168 83 596.3 0 83 169 82 596.3 0 82 170 97 596.3 0 97 171 111 596.3 0 111 172 69 596.3 0 69 173 75 596.3 0 75 174 63 596.3 0 63 175 75 596.3 0 75 176 80 596.3 0 80 177 89 596.3 0 89 178 101 596.3 0 101 179 98 596.3 0 98 180 95 596.3 0 95 181 123 596.3 0 123 182 896 596.3 0 896 183 742 596.3 0 742 184 1239 596.3 0 1239 185 72 596.3 0 72 186 56 596.3 0 56 187 74 596.3 0 74 188 90 596.3 0 90 189 79 596.3 0 79 190 72 596.3 0 72 191 65 596.3 0 65 192 86 596.3 0 86 193 64 596.3 0 64 194 73 596.3 0 73 195 94 596.3 0 94 196 75 596.3 0 75 197 103 596.3 0 103 198 68 596.3 0 68 199 82 596.3 0 82 200 81 596.3 0 81 201 83 596.3 0 83 202 86 596.3 0 86 203 101 596.3 0 101 204 87 596.3 0 87 205 89 596.3 0 89 206 112 596.3 0 112 207 114 596.3 0 114 208 108 596.3 0 108 209 106 596.3 0 106 210 101 596.3 0 101 211 115 596.3 0 115 212 107 596.3 0 107 213 90 596.3 0 90 214 87 596.3 0 87 215 88 596.3 0 88 216 105 596.3 0 105 217 78 596.3 0 78 218 103 596.3 0 103 219 89 596.3 0 89 220 67 596.3 0 67 221 115 596.3 0 115 222 89 596.3 0 89 223 98 596.3 0 98 224 96 596.3 0 96 225 133 596.3 0 133 226 253 596.3 0 253 227 186 596.3 0 186 228 158 596.3 0 158 229 70 596.3 0 70 230 76 596.3 0 76 231 92 596.3 0 92 232 109 596.3 0 109 233 107 596.3 0 107 234 95 596.3 0 95 235 104 596.3 0 104 236 171 596.3 0 171 237 103 596.3 0 103 238 126 596.3 0 126 239 94 596.3 0 94 240 98 596.3 0 98 241 98 596.3 0 98 242 104 596.3 0 104 243 107 596.3 0 107 244 74 596.3 0 74 245 82 596.3 0 82 246 132 596.3 0 132 247 99 596.3 0 99 248 160 596.3 0 160 249 201 596.3 0 201 250 195 596.3 0 195 251 247 596.3 0 247 252 306 596.3 0 306 253 3048 596.3 0 3048 254 3198 596.3 0 3198 255 15944 596.3 0 15944 256 562 596.3 0 562 257 320 596.3 0 320 258 65 596.3 0 65 259 74 596.3 0 74 260 125 596.3 0 125 261 83 596.3 0 83 262 74 596.3 0 74 263 77 596.3 0 77 264 125 596.3 0 125 265 118 596.3 0 118 266 151 596.3 0 151 267 49 596.3 0 49 268 65 596.3 0 65 269 71 596.3 0 71 270 73 596.3 0 73 271 76 596.3 0 76 272 70 596.3 0 70 273 80 596.3 0 80 274 73 596.3 0 73 275 84 596.3 0 84 276 115 596.3 0 115 277 90 596.3 0 90 278 78 596.3 0 78 279 59 596.3 0 59 280 68 596.3 0 68 281 66 596.3 0 66 282 70 596.3 0 70 283 65 596.3 0 65 284 79 596.3 0 79 285 64 596.3 0 64 286 94 596.3 0 94 287 69 596.3 0 69 288 64 596.3 0 64 289 105 596.3 0 105 290 98 596.3 0 98 291 95 596.3 0 95 292 63 596.3 0 63 293 77 596.3 0 77 294 66 596.3 0 66 295 69 596.3 0 69 296 65 596.3 0 65 297 67 596.3 0 67 298 86 596.3 0 86 299 73 596.3 0 73 300 75 596.3 0 75 301 102 596.3 0 102 Finished in 2.60 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 58,827 Reads with adapters: 13,091 (22.3%) Reads that were too short: 120 (0.2%) Reads written (passing filters): 12,971 (22.0%) Total basepairs processed: 13,996,564 bp Total written (filtered): 3,572,691 bp (25.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13091 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 81 0.0 3 0 79 2 17 12791 0.0 3 12542 236 10 3 18 30 0.0 3 2 26 2 19 6 0.0 3 0 0 4 2 20 10 0.0 3 0 0 0 10 21 2 0.0 3 0 0 0 2 25 6 0.0 3 0 0 1 5 27 1 0.0 3 0 0 0 1 28 4 0.0 3 0 0 0 4 37 1 0.0 3 0 0 0 1 38 1 0.0 3 1 41 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 52 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 112 8 0.0 3 0 0 0 8 114 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 172 4 0.0 3 0 0 0 4 175 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 214 5 0.0 3 0 0 0 5 216 2 0.0 3 0 0 0 2 217 84 0.0 3 0 0 2 82 221 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 12,971 Reads with adapters: 42 (0.3%) Reads written (passing filters): 12,971 (100.0%) Total basepairs processed: 3,572,691 bp Total written (filtered): 3,572,554 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 42 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.2% C: 16.7% G: 28.6% T: 9.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 31 202.7 0 31 4 11 50.7 0 11