This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.26 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 125,753 (5.1%) Reads that were too short: 11,580 (0.5%) Reads written (passing filters): 114,173 (4.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 32,388,436 bp (4.4%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 125753 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1684 596.3 0 1684 7 56333 596.3 0 56333 8 46319 596.3 0 46319 9 73 596.3 0 73 10 18 596.3 0 18 11 21 596.3 0 21 12 16 596.3 0 16 13 33 596.3 0 33 14 27 596.3 0 27 15 20 596.3 0 20 16 43 596.3 0 43 17 23 596.3 0 23 18 25 596.3 0 25 19 22 596.3 0 22 20 20 596.3 0 20 21 20 596.3 0 20 22 24 596.3 0 24 23 19 596.3 0 19 24 21 596.3 0 21 25 20 596.3 0 20 26 108 596.3 0 108 27 59 596.3 0 59 28 112 596.3 0 112 29 16 596.3 0 16 30 12 596.3 0 12 31 22 596.3 0 22 32 25 596.3 0 25 33 18 596.3 0 18 34 13 596.3 0 13 35 25 596.3 0 25 36 17 596.3 0 17 37 28 596.3 0 28 38 20 596.3 0 20 39 23 596.3 0 23 40 20 596.3 0 20 41 21 596.3 0 21 42 23 596.3 0 23 43 17 596.3 0 17 44 13 596.3 0 13 45 25 596.3 0 25 46 11 596.3 0 11 47 21 596.3 0 21 48 15 596.3 0 15 49 22 596.3 0 22 50 12 596.3 0 12 51 27 596.3 0 27 52 20 596.3 0 20 53 19 596.3 0 19 54 21 596.3 0 21 55 19 596.3 0 19 56 13 596.3 0 13 57 15 596.3 0 15 58 15 596.3 0 15 59 21 596.3 0 21 60 23 596.3 0 23 61 19 596.3 0 19 62 18 596.3 0 18 63 23 596.3 0 23 64 16 596.3 0 16 65 24 596.3 0 24 66 13 596.3 0 13 67 19 596.3 0 19 68 22 596.3 0 22 69 27 596.3 0 27 70 13 596.3 0 13 71 18 596.3 0 18 72 13 596.3 0 13 73 17 596.3 0 17 74 17 596.3 0 17 75 49 596.3 0 49 76 27 596.3 0 27 77 48 596.3 0 48 78 33 596.3 0 33 79 35 596.3 0 35 80 37 596.3 0 37 81 35 596.3 0 35 82 30 596.3 0 30 83 27 596.3 0 27 84 23 596.3 0 23 85 29 596.3 0 29 86 79 596.3 0 79 87 19 596.3 0 19 88 18 596.3 0 18 89 20 596.3 0 20 90 20 596.3 0 20 91 29 596.3 0 29 92 31 596.3 0 31 93 16 596.3 0 16 94 32 596.3 0 32 95 32 596.3 0 32 96 42 596.3 0 42 97 42 596.3 0 42 98 35 596.3 0 35 99 50 596.3 0 50 100 89 596.3 0 89 101 73 596.3 0 73 102 51 596.3 0 51 103 52 596.3 0 52 104 36 596.3 0 36 105 73 596.3 0 73 106 107 596.3 0 107 107 71 596.3 0 71 108 297 596.3 0 297 109 226 596.3 0 226 110 445 596.3 0 445 111 85 596.3 0 85 112 65 596.3 0 65 113 83 596.3 0 83 114 127 596.3 0 127 115 74 596.3 0 74 116 146 596.3 0 146 117 46 596.3 0 46 118 37 596.3 0 37 119 39 596.3 0 39 120 30 596.3 0 30 121 41 596.3 0 41 122 61 596.3 0 61 123 66 596.3 0 66 124 48 596.3 0 48 125 39 596.3 0 39 126 47 596.3 0 47 127 33 596.3 0 33 128 42 596.3 0 42 129 57 596.3 0 57 130 69 596.3 0 69 131 93 596.3 0 93 132 59 596.3 0 59 133 56 596.3 0 56 134 59 596.3 0 59 135 73 596.3 0 73 136 101 596.3 0 101 137 88 596.3 0 88 138 67 596.3 0 67 139 80 596.3 0 80 140 73 596.3 0 73 141 78 596.3 0 78 142 52 596.3 0 52 143 67 596.3 0 67 144 60 596.3 0 60 145 85 596.3 0 85 146 56 596.3 0 56 147 38 596.3 0 38 148 65 596.3 0 65 149 44 596.3 0 44 150 47 596.3 0 47 151 43 596.3 0 43 152 30 596.3 0 30 153 58 596.3 0 58 154 57 596.3 0 57 155 112 596.3 0 112 156 188 596.3 0 188 157 110 596.3 0 110 158 77 596.3 0 77 159 165 596.3 0 165 160 71 596.3 0 71 161 59 596.3 0 59 162 66 596.3 0 66 163 156 596.3 0 156 164 78 596.3 0 78 165 85 596.3 0 85 166 67 596.3 0 67 167 67 596.3 0 67 168 52 596.3 0 52 169 67 596.3 0 67 170 52 596.3 0 52 171 44 596.3 0 44 172 35 596.3 0 35 173 40 596.3 0 40 174 37 596.3 0 37 175 39 596.3 0 39 176 59 596.3 0 59 177 46 596.3 0 46 178 72 596.3 0 72 179 64 596.3 0 64 180 56 596.3 0 56 181 53 596.3 0 53 182 58 596.3 0 58 183 60 596.3 0 60 184 67 596.3 0 67 185 67 596.3 0 67 186 45 596.3 0 45 187 50 596.3 0 50 188 61 596.3 0 61 189 59 596.3 0 59 190 52 596.3 0 52 191 47 596.3 0 47 192 79 596.3 0 79 193 44 596.3 0 44 194 48 596.3 0 48 195 50 596.3 0 50 196 60 596.3 0 60 197 48 596.3 0 48 198 43 596.3 0 43 199 51 596.3 0 51 200 51 596.3 0 51 201 63 596.3 0 63 202 134 596.3 0 134 203 91 596.3 0 91 204 77 596.3 0 77 205 67 596.3 0 67 206 40 596.3 0 40 207 59 596.3 0 59 208 112 596.3 0 112 209 142 596.3 0 142 210 131 596.3 0 131 211 93 596.3 0 93 212 40 596.3 0 40 213 56 596.3 0 56 214 38 596.3 0 38 215 51 596.3 0 51 216 64 596.3 0 64 217 47 596.3 0 47 218 84 596.3 0 84 219 39 596.3 0 39 220 54 596.3 0 54 221 58 596.3 0 58 222 87 596.3 0 87 223 134 596.3 0 134 224 104 596.3 0 104 225 100 596.3 0 100 226 96 596.3 0 96 227 72 596.3 0 72 228 45 596.3 0 45 229 50 596.3 0 50 230 52 596.3 0 52 231 37 596.3 0 37 232 59 596.3 0 59 233 46 596.3 0 46 234 50 596.3 0 50 235 60 596.3 0 60 236 70 596.3 0 70 237 48 596.3 0 48 238 56 596.3 0 56 239 110 596.3 0 110 240 68 596.3 0 68 241 65 596.3 0 65 242 263 596.3 0 263 243 113 596.3 0 113 244 194 596.3 0 194 245 140 596.3 0 140 246 243 596.3 0 243 247 56 596.3 0 56 248 54 596.3 0 54 249 54 596.3 0 54 250 64 596.3 0 64 251 45 596.3 0 45 252 92 596.3 0 92 253 294 596.3 0 294 254 206 596.3 0 206 255 716 596.3 0 716 256 94 596.3 0 94 257 202 596.3 0 202 258 744 596.3 0 744 259 559 596.3 0 559 260 584 596.3 0 584 261 62 596.3 0 62 262 66 596.3 0 66 263 123 596.3 0 123 264 152 596.3 0 152 265 207 596.3 0 207 266 39 596.3 0 39 267 44 596.3 0 44 268 37 596.3 0 37 269 83 596.3 0 83 270 99 596.3 0 99 271 210 596.3 0 210 272 113 596.3 0 113 273 236 596.3 0 236 274 80 596.3 0 80 275 51 596.3 0 51 276 79 596.3 0 79 277 77 596.3 0 77 278 68 596.3 0 68 279 48 596.3 0 48 280 63 596.3 0 63 281 78 596.3 0 78 282 64 596.3 0 64 283 103 596.3 0 103 284 126 596.3 0 126 285 143 596.3 0 143 286 91 596.3 0 91 287 54 596.3 0 54 288 46 596.3 0 46 289 53 596.3 0 53 290 143 596.3 0 143 291 254 596.3 0 254 292 196 596.3 0 196 293 67 596.3 0 67 294 59 596.3 0 59 295 62 596.3 0 62 296 58 596.3 0 58 297 74 596.3 0 74 298 86 596.3 0 86 299 93 596.3 0 93 300 70 596.3 0 70 301 120 596.3 0 120 Finished in 6.01 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 114,173 Reads with adapters: 48,351 (42.3%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 48,301 (42.3%) Total basepairs processed: 32,388,436 bp Total written (filtered): 13,077,958 bp (40.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 48351 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 1 4 18 17 0.0 3 0 0 10 2 5 19 466 0.0 3 0 365 54 34 13 20 46452 0.0 4 43599 2309 398 105 41 21 125 0.0 4 23 92 4 4 2 22 22 0.0 4 7 2 9 1 3 23 182 0.0 4 0 0 1 139 42 24 1 0.0 4 0 0 0 1 26 22 0.0 4 0 0 0 0 22 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 39 1 0.0 4 1 77 1 0.0 4 0 0 0 1 83 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 14 0.0 4 0 0 0 0 14 127 970 0.0 4 0 0 0 0 970 128 7 0.0 4 0 0 0 0 7 130 7 0.0 4 0 0 0 0 7 138 1 0.0 4 0 0 0 1 153 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 189 2 0.0 4 0 0 0 0 2 199 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 16 0.0 4 0 0 0 0 16 229 7 0.0 4 0 0 0 0 7 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 290 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.75 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 48,301 Reads with adapters: 8,592 (17.8%) Reads written (passing filters): 48,301 (100.0%) Total basepairs processed: 13,077,958 bp Total written (filtered): 12,981,654 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8592 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 9.5% G: 85.3% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 81 754.7 0 81 4 29 188.7 0 29 8 2 0.7 0 2 9 137 0.2 0 11 126 10 1235 0.0 1 85 1150 11 6976 0.0 1 288 6688 12 86 0.0 1 7 79 13 11 0.0 1 0 11 14 8 0.0 1 0 8 15 2 0.0 1 0 2 122 1 0.0 1 1 123 5 0.0 1 4 1 151 7 0.0 1 5 2 152 1 0.0 1 1 180 1 0.0 1 1 209 5 0.0 1 5 224 4 0.0 1 3 1 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.