This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.17 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 125,679 (5.1%) Reads that were too short: 9,924 (0.4%) Reads written (passing filters): 115,755 (4.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 33,086,136 bp (4.5%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 125679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 880 596.3 0 880 7 50645 596.3 0 50645 8 56957 596.3 0 56957 9 33 596.3 0 33 10 16 596.3 0 16 11 17 596.3 0 17 12 10 596.3 0 10 13 15 596.3 0 15 14 12 596.3 0 12 15 13 596.3 0 13 16 21 596.3 0 21 17 16 596.3 0 16 18 17 596.3 0 17 19 7 596.3 0 7 20 9 596.3 0 9 21 8 596.3 0 8 22 9 596.3 0 9 23 16 596.3 0 16 24 15 596.3 0 15 25 5 596.3 0 5 26 13 596.3 0 13 27 10 596.3 0 10 28 26 596.3 0 26 29 13 596.3 0 13 30 10 596.3 0 10 31 7 596.3 0 7 32 5 596.3 0 5 33 17 596.3 0 17 34 10 596.3 0 10 35 15 596.3 0 15 36 12 596.3 0 12 37 16 596.3 0 16 38 11 596.3 0 11 39 14 596.3 0 14 40 11 596.3 0 11 41 10 596.3 0 10 42 10 596.3 0 10 43 4 596.3 0 4 44 9 596.3 0 9 45 9 596.3 0 9 46 9 596.3 0 9 47 9 596.3 0 9 48 6 596.3 0 6 49 14 596.3 0 14 50 10 596.3 0 10 51 9 596.3 0 9 52 9 596.3 0 9 53 6 596.3 0 6 54 8 596.3 0 8 55 8 596.3 0 8 56 10 596.3 0 10 57 10 596.3 0 10 58 9 596.3 0 9 59 16 596.3 0 16 60 23 596.3 0 23 61 7 596.3 0 7 62 15 596.3 0 15 63 21 596.3 0 21 64 6 596.3 0 6 65 16 596.3 0 16 66 11 596.3 0 11 67 17 596.3 0 17 68 9 596.3 0 9 69 8 596.3 0 8 70 11 596.3 0 11 71 15 596.3 0 15 72 13 596.3 0 13 73 8 596.3 0 8 74 13 596.3 0 13 75 14 596.3 0 14 76 14 596.3 0 14 77 20 596.3 0 20 78 9 596.3 0 9 79 13 596.3 0 13 80 12 596.3 0 12 81 27 596.3 0 27 82 21 596.3 0 21 83 25 596.3 0 25 84 16 596.3 0 16 85 24 596.3 0 24 86 90 596.3 0 90 87 26 596.3 0 26 88 23 596.3 0 23 89 28 596.3 0 28 90 27 596.3 0 27 91 19 596.3 0 19 92 41 596.3 0 41 93 24 596.3 0 24 94 25 596.3 0 25 95 24 596.3 0 24 96 55 596.3 0 55 97 38 596.3 0 38 98 30 596.3 0 30 99 38 596.3 0 38 100 55 596.3 0 55 101 33 596.3 0 33 102 23 596.3 0 23 103 24 596.3 0 24 104 24 596.3 0 24 105 42 596.3 0 42 106 110 596.3 0 110 107 69 596.3 0 69 108 173 596.3 0 173 109 147 596.3 0 147 110 297 596.3 0 297 111 53 596.3 0 53 112 66 596.3 0 66 113 58 596.3 0 58 114 129 596.3 0 129 115 73 596.3 0 73 116 112 596.3 0 112 117 45 596.3 0 45 118 30 596.3 0 30 119 27 596.3 0 27 120 17 596.3 0 17 121 27 596.3 0 27 122 44 596.3 0 44 123 48 596.3 0 48 124 44 596.3 0 44 125 26 596.3 0 26 126 37 596.3 0 37 127 27 596.3 0 27 128 50 596.3 0 50 129 71 596.3 0 71 130 53 596.3 0 53 131 85 596.3 0 85 132 38 596.3 0 38 133 40 596.3 0 40 134 47 596.3 0 47 135 48 596.3 0 48 136 77 596.3 0 77 137 75 596.3 0 75 138 40 596.3 0 40 139 47 596.3 0 47 140 47 596.3 0 47 141 58 596.3 0 58 142 50 596.3 0 50 143 45 596.3 0 45 144 44 596.3 0 44 145 54 596.3 0 54 146 33 596.3 0 33 147 39 596.3 0 39 148 42 596.3 0 42 149 27 596.3 0 27 150 48 596.3 0 48 151 38 596.3 0 38 152 48 596.3 0 48 153 58 596.3 0 58 154 41 596.3 0 41 155 111 596.3 0 111 156 131 596.3 0 131 157 141 596.3 0 141 158 57 596.3 0 57 159 137 596.3 0 137 160 46 596.3 0 46 161 53 596.3 0 53 162 45 596.3 0 45 163 156 596.3 0 156 164 56 596.3 0 56 165 76 596.3 0 76 166 42 596.3 0 42 167 54 596.3 0 54 168 44 596.3 0 44 169 52 596.3 0 52 170 33 596.3 0 33 171 40 596.3 0 40 172 35 596.3 0 35 173 29 596.3 0 29 174 25 596.3 0 25 175 34 596.3 0 34 176 65 596.3 0 65 177 36 596.3 0 36 178 48 596.3 0 48 179 46 596.3 0 46 180 48 596.3 0 48 181 51 596.3 0 51 182 35 596.3 0 35 183 47 596.3 0 47 184 44 596.3 0 44 185 43 596.3 0 43 186 43 596.3 0 43 187 36 596.3 0 36 188 28 596.3 0 28 189 38 596.3 0 38 190 48 596.3 0 48 191 53 596.3 0 53 192 82 596.3 0 82 193 32 596.3 0 32 194 43 596.3 0 43 195 41 596.3 0 41 196 49 596.3 0 49 197 44 596.3 0 44 198 43 596.3 0 43 199 29 596.3 0 29 200 49 596.3 0 49 201 72 596.3 0 72 202 87 596.3 0 87 203 85 596.3 0 85 204 50 596.3 0 50 205 47 596.3 0 47 206 39 596.3 0 39 207 62 596.3 0 62 208 82 596.3 0 82 209 101 596.3 0 101 210 105 596.3 0 105 211 77 596.3 0 77 212 29 596.3 0 29 213 31 596.3 0 31 214 34 596.3 0 34 215 26 596.3 0 26 216 73 596.3 0 73 217 47 596.3 0 47 218 52 596.3 0 52 219 42 596.3 0 42 220 46 596.3 0 46 221 37 596.3 0 37 222 58 596.3 0 58 223 104 596.3 0 104 224 74 596.3 0 74 225 55 596.3 0 55 226 57 596.3 0 57 227 55 596.3 0 55 228 33 596.3 0 33 229 45 596.3 0 45 230 38 596.3 0 38 231 34 596.3 0 34 232 42 596.3 0 42 233 38 596.3 0 38 234 33 596.3 0 33 235 54 596.3 0 54 236 44 596.3 0 44 237 50 596.3 0 50 238 53 596.3 0 53 239 110 596.3 0 110 240 64 596.3 0 64 241 59 596.3 0 59 242 235 596.3 0 235 243 108 596.3 0 108 244 164 596.3 0 164 245 117 596.3 0 117 246 191 596.3 0 191 247 38 596.3 0 38 248 60 596.3 0 60 249 49 596.3 0 49 250 41 596.3 0 41 251 45 596.3 0 45 252 91 596.3 0 91 253 252 596.3 0 252 254 175 596.3 0 175 255 424 596.3 0 424 256 71 596.3 0 71 257 212 596.3 0 212 258 986 596.3 0 986 259 719 596.3 0 719 260 765 596.3 0 765 261 47 596.3 0 47 262 55 596.3 0 55 263 91 596.3 0 91 264 112 596.3 0 112 265 130 596.3 0 130 266 41 596.3 0 41 267 21 596.3 0 21 268 36 596.3 0 36 269 48 596.3 0 48 270 77 596.3 0 77 271 142 596.3 0 142 272 113 596.3 0 113 273 207 596.3 0 207 274 50 596.3 0 50 275 52 596.3 0 52 276 42 596.3 0 42 277 53 596.3 0 53 278 49 596.3 0 49 279 48 596.3 0 48 280 34 596.3 0 34 281 68 596.3 0 68 282 65 596.3 0 65 283 72 596.3 0 72 284 77 596.3 0 77 285 68 596.3 0 68 286 66 596.3 0 66 287 46 596.3 0 46 288 40 596.3 0 40 289 39 596.3 0 39 290 102 596.3 0 102 291 226 596.3 0 226 292 138 596.3 0 138 293 48 596.3 0 48 294 44 596.3 0 44 295 42 596.3 0 42 296 48 596.3 0 48 297 47 596.3 0 47 298 80 596.3 0 80 299 60 596.3 0 60 300 52 596.3 0 52 301 53 596.3 0 53 Finished in 5.51 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 115,755 Reads with adapters: 50,560 (43.7%) Reads that were too short: 84 (0.1%) Reads written (passing filters): 50,476 (43.6%) Total basepairs processed: 33,086,136 bp Total written (filtered): 13,979,745 bp (42.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50560 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 208 0.0 3 0 203 4 1 17 50091 0.0 3 49023 1036 31 1 18 104 0.0 3 11 92 1 19 23 0.0 3 2 0 19 2 20 27 0.0 3 0 0 0 27 29 3 0.0 3 0 0 0 3 31 1 0.0 3 1 36 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 217 61 0.0 3 0 0 1 60 218 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.78 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 50,476 Reads with adapters: 367 (0.7%) Reads written (passing filters): 50,476 (100.0%) Total basepairs processed: 13,979,745 bp Total written (filtered): 13,978,619 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 367 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.9% C: 33.0% G: 7.9% T: 12.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 353 788.7 0 353 4 8 197.2 0 8 5 3 49.3 0 3 6 1 12.3 0 1 7 2 3.1 0 2