This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.53 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 103,097 (4.2%) Reads that were too short: 36,306 (1.5%) Reads written (passing filters): 66,791 (2.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 17,482,803 bp (2.4%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 103097 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2667 596.3 0 2667 7 16788 596.3 0 16788 8 728 596.3 0 728 9 26586 596.3 0 26586 10 74 596.3 0 74 11 33 596.3 0 33 12 39 596.3 0 39 13 35 596.3 0 35 14 40 596.3 0 40 15 47 596.3 0 47 16 26 596.3 0 26 17 54 596.3 0 54 18 124 596.3 0 124 19 93 596.3 0 93 20 42 596.3 0 42 21 34 596.3 0 34 22 31 596.3 0 31 23 36 596.3 0 36 24 35 596.3 0 35 25 28 596.3 0 28 26 24 596.3 0 24 27 41 596.3 0 41 28 34 596.3 0 34 29 33 596.3 0 33 30 36 596.3 0 36 31 40 596.3 0 40 32 43 596.3 0 43 33 37 596.3 0 37 34 27 596.3 0 27 35 38 596.3 0 38 36 33 596.3 0 33 37 47 596.3 0 47 38 42 596.3 0 42 39 50 596.3 0 50 40 44 596.3 0 44 41 41 596.3 0 41 42 44 596.3 0 44 43 62 596.3 0 62 44 39 596.3 0 39 45 45 596.3 0 45 46 39 596.3 0 39 47 506 596.3 0 506 48 396 596.3 0 396 49 531 596.3 0 531 50 135 596.3 0 135 51 43 596.3 0 43 52 41 596.3 0 41 53 47 596.3 0 47 54 31 596.3 0 31 55 51 596.3 0 51 56 39 596.3 0 39 57 56 596.3 0 56 58 53 596.3 0 53 59 132 596.3 0 132 60 142 596.3 0 142 61 171 596.3 0 171 62 59 596.3 0 59 63 67 596.3 0 67 64 53 596.3 0 53 65 31 596.3 0 31 66 34 596.3 0 34 67 36 596.3 0 36 68 38 596.3 0 38 69 47 596.3 0 47 70 40 596.3 0 40 71 66 596.3 0 66 72 121 596.3 0 121 73 53 596.3 0 53 74 53 596.3 0 53 75 46 596.3 0 46 76 58 596.3 0 58 77 278 596.3 0 278 78 258 596.3 0 258 79 257 596.3 0 257 80 47 596.3 0 47 81 43 596.3 0 43 82 91 596.3 0 91 83 65 596.3 0 65 84 365 596.3 0 365 85 544 596.3 0 544 86 439 596.3 0 439 87 32 596.3 0 32 88 31 596.3 0 31 89 34 596.3 0 34 90 21 596.3 0 21 91 50 596.3 0 50 92 42 596.3 0 42 93 40 596.3 0 40 94 32 596.3 0 32 95 23 596.3 0 23 96 39 596.3 0 39 97 44 596.3 0 44 98 33 596.3 0 33 99 45 596.3 0 45 100 35 596.3 0 35 101 35 596.3 0 35 102 21 596.3 0 21 103 27 596.3 0 27 104 28 596.3 0 28 105 26 596.3 0 26 106 35 596.3 0 35 107 56 596.3 0 56 108 38 596.3 0 38 109 45 596.3 0 45 110 49 596.3 0 49 111 65 596.3 0 65 112 683 596.3 0 683 113 128 596.3 0 128 114 372 596.3 0 372 115 45 596.3 0 45 116 44 596.3 0 44 117 68 596.3 0 68 118 72 596.3 0 72 119 87 596.3 0 87 120 38 596.3 0 38 121 28 596.3 0 28 122 26 596.3 0 26 123 34 596.3 0 34 124 28 596.3 0 28 125 36 596.3 0 36 126 39 596.3 0 39 127 100 596.3 0 100 128 168 596.3 0 168 129 204 596.3 0 204 130 80 596.3 0 80 131 127 596.3 0 127 132 288 596.3 0 288 133 454 596.3 0 454 134 523 596.3 0 523 135 229 596.3 0 229 136 108 596.3 0 108 137 83 596.3 0 83 138 101 596.3 0 101 139 75 596.3 0 75 140 132 596.3 0 132 141 1250 596.3 0 1250 142 1306 596.3 0 1306 143 1147 596.3 0 1147 144 50 596.3 0 50 145 56 596.3 0 56 146 43 596.3 0 43 147 84 596.3 0 84 148 62 596.3 0 62 149 85 596.3 0 85 150 46 596.3 0 46 151 60 596.3 0 60 152 221 596.3 0 221 153 408 596.3 0 408 154 249 596.3 0 249 155 102 596.3 0 102 156 21 596.3 0 21 157 27 596.3 0 27 158 33 596.3 0 33 159 33 596.3 0 33 160 31 596.3 0 31 161 27 596.3 0 27 162 34 596.3 0 34 163 34 596.3 0 34 164 41 596.3 0 41 165 27 596.3 0 27 166 31 596.3 0 31 167 26 596.3 0 26 168 33 596.3 0 33 169 39 596.3 0 39 170 38 596.3 0 38 171 37 596.3 0 37 172 47 596.3 0 47 173 53 596.3 0 53 174 47 596.3 0 47 175 50 596.3 0 50 176 47 596.3 0 47 177 30 596.3 0 30 178 36 596.3 0 36 179 34 596.3 0 34 180 41 596.3 0 41 181 35 596.3 0 35 182 37 596.3 0 37 183 54 596.3 0 54 184 46 596.3 0 46 185 82 596.3 0 82 186 78 596.3 0 78 187 196 596.3 0 196 188 42 596.3 0 42 189 57 596.3 0 57 190 70 596.3 0 70 191 88 596.3 0 88 192 65 596.3 0 65 193 110 596.3 0 110 194 66 596.3 0 66 195 91 596.3 0 91 196 48 596.3 0 48 197 41 596.3 0 41 198 50 596.3 0 50 199 69 596.3 0 69 200 66 596.3 0 66 201 66 596.3 0 66 202 47 596.3 0 47 203 44 596.3 0 44 204 41 596.3 0 41 205 87 596.3 0 87 206 55 596.3 0 55 207 56 596.3 0 56 208 51 596.3 0 51 209 32 596.3 0 32 210 64 596.3 0 64 211 75 596.3 0 75 212 137 596.3 0 137 213 204 596.3 0 204 214 185 596.3 0 185 215 134 596.3 0 134 216 149 596.3 0 149 217 118 596.3 0 118 218 227 596.3 0 227 219 321 596.3 0 321 220 239 596.3 0 239 221 116 596.3 0 116 222 55 596.3 0 55 223 45 596.3 0 45 224 94 596.3 0 94 225 110 596.3 0 110 226 107 596.3 0 107 227 100 596.3 0 100 228 140 596.3 0 140 229 162 596.3 0 162 230 258 596.3 0 258 231 209 596.3 0 209 232 212 596.3 0 212 233 131 596.3 0 131 234 182 596.3 0 182 235 596 596.3 0 596 236 1112 596.3 0 1112 237 978 596.3 0 978 238 729 596.3 0 729 239 511 596.3 0 511 240 306 596.3 0 306 241 112 596.3 0 112 242 68 596.3 0 68 243 134 596.3 0 134 244 234 596.3 0 234 245 344 596.3 0 344 246 580 596.3 0 580 247 471 596.3 0 471 248 400 596.3 0 400 249 264 596.3 0 264 250 222 596.3 0 222 251 269 596.3 0 269 252 453 596.3 0 453 253 565 596.3 0 565 254 471 596.3 0 471 255 418 596.3 0 418 256 1005 596.3 0 1005 257 885 596.3 0 885 258 403 596.3 0 403 259 106 596.3 0 106 260 210 596.3 0 210 261 147 596.3 0 147 262 197 596.3 0 197 263 142 596.3 0 142 264 104 596.3 0 104 265 85 596.3 0 85 266 164 596.3 0 164 267 183 596.3 0 183 268 112 596.3 0 112 269 69 596.3 0 69 270 91 596.3 0 91 271 90 596.3 0 90 272 132 596.3 0 132 273 736 596.3 0 736 274 626 596.3 0 626 275 228 596.3 0 228 276 119 596.3 0 119 277 88 596.3 0 88 278 116 596.3 0 116 279 165 596.3 0 165 280 181 596.3 0 181 281 169 596.3 0 169 282 179 596.3 0 179 283 250 596.3 0 250 284 640 596.3 0 640 285 707 596.3 0 707 286 551 596.3 0 551 287 539 596.3 0 539 288 412 596.3 0 412 289 247 596.3 0 247 290 171 596.3 0 171 291 190 596.3 0 190 292 185 596.3 0 185 293 330 596.3 0 330 294 211 596.3 0 211 295 241 596.3 0 241 296 458 596.3 0 458 297 517 596.3 0 517 298 3061 596.3 0 3061 299 3460 596.3 0 3460 300 3252 596.3 0 3252 301 728 596.3 0 728 Finished in 3.57 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 66,791 Reads with adapters: 18,062 (27.0%) Reads that were too short: 44 (0.1%) Reads written (passing filters): 18,018 (27.0%) Total basepairs processed: 17,482,803 bp Total written (filtered): 4,858,891 bp (27.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18062 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 3 6 18 16 0.0 3 0 0 9 2 5 19 287 0.0 3 0 178 41 35 33 20 15871 0.0 4 13544 1589 479 179 80 21 85 0.0 4 20 45 7 10 3 22 950 0.0 4 848 71 18 6 7 23 82 0.0 4 0 0 2 36 44 24 1 0.0 4 0 0 0 0 1 25 9 0.0 4 0 0 0 8 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 1 53 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 0 2 90 2 0.0 4 0 0 0 0 2 100 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 0 5 127 680 0.0 4 0 0 0 0 680 128 2 0.0 4 0 0 0 0 2 129 3 0.0 4 0 0 0 0 3 130 2 0.0 4 0 0 0 0 2 132 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 1 151 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 1 8 229 3 0.0 4 0 0 0 1 2 231 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 18,018 Reads with adapters: 1,021 (5.7%) Reads written (passing filters): 18,018 (100.0%) Total basepairs processed: 4,858,891 bp Total written (filtered): 4,845,710 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1021 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.6% C: 15.7% G: 74.2% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 111 281.5 0 111 4 8 70.4 0 8 9 49 0.1 0 1 48 10 191 0.0 1 21 170 11 293 0.0 1 8 285 12 330 0.0 1 8 322 13 18 0.0 1 1 17 14 2 0.0 1 0 2 15 3 0.0 1 0 3 45 1 0.0 1 0 1 92 1 0.0 1 1 93 1 0.0 1 0 1 140 1 0.0 1 1 152 1 0.0 1 1 153 1 0.0 1 0 1 191 1 0.0 1 0 1 223 3 0.0 1 3 224 1 0.0 1 1 225 3 0.0 1 3 270 1 0.0 1 1 271 1 0.0 1 1