This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.96 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 81,001 (3.3%) Reads that were too short: 16,227 (0.7%) Reads written (passing filters): 64,774 (2.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 17,325,547 bp (2.4%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 81001 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2303 596.3 0 2303 7 21060 596.3 0 21060 8 577 596.3 0 577 9 25473 596.3 0 25473 10 61 596.3 0 61 11 33 596.3 0 33 12 41 596.3 0 41 13 44 596.3 0 44 14 36 596.3 0 36 15 43 596.3 0 43 16 27 596.3 0 27 17 40 596.3 0 40 18 64 596.3 0 64 19 54 596.3 0 54 20 31 596.3 0 31 21 31 596.3 0 31 22 42 596.3 0 42 23 38 596.3 0 38 24 35 596.3 0 35 25 29 596.3 0 29 26 27 596.3 0 27 27 48 596.3 0 48 28 36 596.3 0 36 29 25 596.3 0 25 30 37 596.3 0 37 31 39 596.3 0 39 32 26 596.3 0 26 33 51 596.3 0 51 34 37 596.3 0 37 35 40 596.3 0 40 36 39 596.3 0 39 37 48 596.3 0 48 38 35 596.3 0 35 39 35 596.3 0 35 40 38 596.3 0 38 41 45 596.3 0 45 42 36 596.3 0 36 43 38 596.3 0 38 44 35 596.3 0 35 45 46 596.3 0 46 46 43 596.3 0 43 47 159 596.3 0 159 48 122 596.3 0 122 49 215 596.3 0 215 50 55 596.3 0 55 51 40 596.3 0 40 52 41 596.3 0 41 53 44 596.3 0 44 54 53 596.3 0 53 55 51 596.3 0 51 56 54 596.3 0 54 57 49 596.3 0 49 58 56 596.3 0 56 59 97 596.3 0 97 60 86 596.3 0 86 61 119 596.3 0 119 62 44 596.3 0 44 63 73 596.3 0 73 64 41 596.3 0 41 65 34 596.3 0 34 66 32 596.3 0 32 67 35 596.3 0 35 68 41 596.3 0 41 69 49 596.3 0 49 70 38 596.3 0 38 71 45 596.3 0 45 72 88 596.3 0 88 73 42 596.3 0 42 74 44 596.3 0 44 75 46 596.3 0 46 76 52 596.3 0 52 77 108 596.3 0 108 78 97 596.3 0 97 79 87 596.3 0 87 80 44 596.3 0 44 81 39 596.3 0 39 82 57 596.3 0 57 83 48 596.3 0 48 84 209 596.3 0 209 85 285 596.3 0 285 86 270 596.3 0 270 87 37 596.3 0 37 88 26 596.3 0 26 89 42 596.3 0 42 90 34 596.3 0 34 91 47 596.3 0 47 92 33 596.3 0 33 93 33 596.3 0 33 94 47 596.3 0 47 95 36 596.3 0 36 96 39 596.3 0 39 97 40 596.3 0 40 98 28 596.3 0 28 99 52 596.3 0 52 100 37 596.3 0 37 101 32 596.3 0 32 102 40 596.3 0 40 103 34 596.3 0 34 104 36 596.3 0 36 105 44 596.3 0 44 106 64 596.3 0 64 107 32 596.3 0 32 108 46 596.3 0 46 109 44 596.3 0 44 110 43 596.3 0 43 111 60 596.3 0 60 112 625 596.3 0 625 113 154 596.3 0 154 114 386 596.3 0 386 115 31 596.3 0 31 116 42 596.3 0 42 117 38 596.3 0 38 118 54 596.3 0 54 119 46 596.3 0 46 120 38 596.3 0 38 121 43 596.3 0 43 122 30 596.3 0 30 123 30 596.3 0 30 124 29 596.3 0 29 125 42 596.3 0 42 126 51 596.3 0 51 127 81 596.3 0 81 128 148 596.3 0 148 129 129 596.3 0 129 130 49 596.3 0 49 131 81 596.3 0 81 132 141 596.3 0 141 133 251 596.3 0 251 134 254 596.3 0 254 135 167 596.3 0 167 136 74 596.3 0 74 137 56 596.3 0 56 138 65 596.3 0 65 139 61 596.3 0 61 140 90 596.3 0 90 141 1065 596.3 0 1065 142 1085 596.3 0 1085 143 956 596.3 0 956 144 44 596.3 0 44 145 45 596.3 0 45 146 39 596.3 0 39 147 68 596.3 0 68 148 48 596.3 0 48 149 64 596.3 0 64 150 39 596.3 0 39 151 50 596.3 0 50 152 132 596.3 0 132 153 187 596.3 0 187 154 172 596.3 0 172 155 65 596.3 0 65 156 49 596.3 0 49 157 39 596.3 0 39 158 39 596.3 0 39 159 43 596.3 0 43 160 35 596.3 0 35 161 35 596.3 0 35 162 46 596.3 0 46 163 38 596.3 0 38 164 51 596.3 0 51 165 45 596.3 0 45 166 47 596.3 0 47 167 52 596.3 0 52 168 35 596.3 0 35 169 52 596.3 0 52 170 34 596.3 0 34 171 38 596.3 0 38 172 64 596.3 0 64 173 49 596.3 0 49 174 68 596.3 0 68 175 39 596.3 0 39 176 19 596.3 0 19 177 28 596.3 0 28 178 43 596.3 0 43 179 40 596.3 0 40 180 49 596.3 0 49 181 35 596.3 0 35 182 52 596.3 0 52 183 64 596.3 0 64 184 50 596.3 0 50 185 91 596.3 0 91 186 97 596.3 0 97 187 309 596.3 0 309 188 39 596.3 0 39 189 48 596.3 0 48 190 43 596.3 0 43 191 66 596.3 0 66 192 44 596.3 0 44 193 65 596.3 0 65 194 26 596.3 0 26 195 34 596.3 0 34 196 38 596.3 0 38 197 39 596.3 0 39 198 36 596.3 0 36 199 36 596.3 0 36 200 29 596.3 0 29 201 50 596.3 0 50 202 52 596.3 0 52 203 36 596.3 0 36 204 38 596.3 0 38 205 44 596.3 0 44 206 40 596.3 0 40 207 29 596.3 0 29 208 34 596.3 0 34 209 46 596.3 0 46 210 41 596.3 0 41 211 46 596.3 0 46 212 67 596.3 0 67 213 65 596.3 0 65 214 59 596.3 0 59 215 45 596.3 0 45 216 69 596.3 0 69 217 58 596.3 0 58 218 56 596.3 0 56 219 61 596.3 0 61 220 65 596.3 0 65 221 37 596.3 0 37 222 29 596.3 0 29 223 32 596.3 0 32 224 57 596.3 0 57 225 53 596.3 0 53 226 82 596.3 0 82 227 54 596.3 0 54 228 108 596.3 0 108 229 125 596.3 0 125 230 172 596.3 0 172 231 92 596.3 0 92 232 85 596.3 0 85 233 54 596.3 0 54 234 59 596.3 0 59 235 231 596.3 0 231 236 405 596.3 0 405 237 363 596.3 0 363 238 247 596.3 0 247 239 129 596.3 0 129 240 66 596.3 0 66 241 44 596.3 0 44 242 44 596.3 0 44 243 49 596.3 0 49 244 50 596.3 0 50 245 89 596.3 0 89 246 124 596.3 0 124 247 141 596.3 0 141 248 126 596.3 0 126 249 78 596.3 0 78 250 88 596.3 0 88 251 103 596.3 0 103 252 138 596.3 0 138 253 135 596.3 0 135 254 128 596.3 0 128 255 112 596.3 0 112 256 391 596.3 0 391 257 403 596.3 0 403 258 225 596.3 0 225 259 50 596.3 0 50 260 137 596.3 0 137 261 116 596.3 0 116 262 170 596.3 0 170 263 108 596.3 0 108 264 33 596.3 0 33 265 30 596.3 0 30 266 70 596.3 0 70 267 106 596.3 0 106 268 47 596.3 0 47 269 47 596.3 0 47 270 63 596.3 0 63 271 43 596.3 0 43 272 87 596.3 0 87 273 816 596.3 0 816 274 655 596.3 0 655 275 230 596.3 0 230 276 84 596.3 0 84 277 31 596.3 0 31 278 56 596.3 0 56 279 63 596.3 0 63 280 63 596.3 0 63 281 64 596.3 0 64 282 71 596.3 0 71 283 78 596.3 0 78 284 183 596.3 0 183 285 211 596.3 0 211 286 158 596.3 0 158 287 200 596.3 0 200 288 167 596.3 0 167 289 91 596.3 0 91 290 84 596.3 0 84 291 107 596.3 0 107 292 102 596.3 0 102 293 110 596.3 0 110 294 87 596.3 0 87 295 114 596.3 0 114 296 176 596.3 0 176 297 187 596.3 0 187 298 1265 596.3 0 1265 299 1732 596.3 0 1732 300 1740 596.3 0 1740 301 296 596.3 0 296 Finished in 3.08 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 64,774 Reads with adapters: 20,037 (30.9%) Reads that were too short: 84 (0.1%) Reads written (passing filters): 19,953 (30.8%) Total basepairs processed: 17,325,547 bp Total written (filtered): 5,470,362 bp (31.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20037 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 132 0.0 3 0 126 6 17 18770 0.0 3 18303 445 21 1 18 49 0.0 3 7 39 3 19 849 0.0 3 823 14 9 3 20 29 0.0 3 2 1 1 25 21 1 0.0 3 1 24 1 0.0 3 1 38 1 0.0 3 0 0 0 1 39 22 0.0 3 0 0 0 22 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 50 28 0.0 3 0 0 0 28 51 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 59 7 0.0 3 0 0 0 7 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 79 13 0.0 3 0 0 0 13 80 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 90 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 25 0.0 3 0 0 0 25 102 4 0.0 3 0 0 0 4 103 2 0.0 3 0 0 1 1 104 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 11 0.0 3 0 0 0 11 144 1 0.0 3 0 0 0 1 145 4 0.0 3 0 0 0 4 149 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 165 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 30 0.0 3 0 0 0 30 218 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.72 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 19,953 Reads with adapters: 510 (2.6%) Reads written (passing filters): 19,953 (100.0%) Total basepairs processed: 5,470,362 bp Total written (filtered): 5,468,801 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 510 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.7% C: 7.8% G: 25.9% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 480 311.8 0 480 4 29 77.9 0 29 5 1 19.5 0 1