This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.22 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 147,111 (6.0%) Reads that were too short: 44,597 (1.8%) Reads written (passing filters): 102,514 (4.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 26,177,780 bp (3.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 147111 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 991 596.3 0 991 7 35560 596.3 0 35560 8 575 596.3 0 575 9 27000 596.3 0 27000 10 46 596.3 0 46 11 15 596.3 0 15 12 33 596.3 0 33 13 16 596.3 0 16 14 7 596.3 0 7 15 21 596.3 0 21 16 18 596.3 0 18 17 10 596.3 0 10 18 11 596.3 0 11 19 9 596.3 0 9 20 14 596.3 0 14 21 10 596.3 0 10 22 14 596.3 0 14 23 7 596.3 0 7 24 12 596.3 0 12 25 9 596.3 0 9 26 17 596.3 0 17 27 15 596.3 0 15 28 15 596.3 0 15 29 6 596.3 0 6 30 25 596.3 0 25 31 12 596.3 0 12 32 11 596.3 0 11 33 17 596.3 0 17 34 15 596.3 0 15 35 12 596.3 0 12 36 15 596.3 0 15 37 14 596.3 0 14 38 16 596.3 0 16 39 7 596.3 0 7 40 11 596.3 0 11 41 12 596.3 0 12 42 23 596.3 0 23 43 19 596.3 0 19 44 17 596.3 0 17 45 25 596.3 0 25 46 20 596.3 0 20 47 16 596.3 0 16 48 17 596.3 0 17 49 11 596.3 0 11 50 9 596.3 0 9 51 13 596.3 0 13 52 23 596.3 0 23 53 23 596.3 0 23 54 26 596.3 0 26 55 127 596.3 0 127 56 57 596.3 0 57 57 26 596.3 0 26 58 8 596.3 0 8 59 40 596.3 0 40 60 20 596.3 0 20 61 33 596.3 0 33 62 40 596.3 0 40 63 27 596.3 0 27 64 40 596.3 0 40 65 109 596.3 0 109 66 33 596.3 0 33 67 33 596.3 0 33 68 20 596.3 0 20 69 100 596.3 0 100 70 73 596.3 0 73 71 48 596.3 0 48 72 46 596.3 0 46 73 46 596.3 0 46 74 85 596.3 0 85 75 125 596.3 0 125 76 126 596.3 0 126 77 126 596.3 0 126 78 176 596.3 0 176 79 180 596.3 0 180 80 270 596.3 0 270 81 415 596.3 0 415 82 3810 596.3 0 3810 83 2669 596.3 0 2669 84 15639 596.3 0 15639 85 143 596.3 0 143 86 60 596.3 0 60 87 18 596.3 0 18 88 39 596.3 0 39 89 28 596.3 0 28 90 14 596.3 0 14 91 18 596.3 0 18 92 37 596.3 0 37 93 21 596.3 0 21 94 15 596.3 0 15 95 14 596.3 0 14 96 11 596.3 0 11 97 28 596.3 0 28 98 15 596.3 0 15 99 21 596.3 0 21 100 11 596.3 0 11 101 15 596.3 0 15 102 43 596.3 0 43 103 41 596.3 0 41 104 28 596.3 0 28 105 15 596.3 0 15 106 24 596.3 0 24 107 19 596.3 0 19 108 10 596.3 0 10 109 17 596.3 0 17 110 15 596.3 0 15 111 21 596.3 0 21 112 28 596.3 0 28 113 37 596.3 0 37 114 38 596.3 0 38 115 32 596.3 0 32 116 27 596.3 0 27 117 19 596.3 0 19 118 28 596.3 0 28 119 33 596.3 0 33 120 29 596.3 0 29 121 147 596.3 0 147 122 190 596.3 0 190 123 320 596.3 0 320 124 209 596.3 0 209 125 70 596.3 0 70 126 43 596.3 0 43 127 67 596.3 0 67 128 137 596.3 0 137 129 27 596.3 0 27 130 22 596.3 0 22 131 26 596.3 0 26 132 29 596.3 0 29 133 54 596.3 0 54 134 44 596.3 0 44 135 23 596.3 0 23 136 99 596.3 0 99 137 33 596.3 0 33 138 87 596.3 0 87 139 80 596.3 0 80 140 329 596.3 0 329 141 36 596.3 0 36 142 22 596.3 0 22 143 24 596.3 0 24 144 31 596.3 0 31 145 24 596.3 0 24 146 40 596.3 0 40 147 50 596.3 0 50 148 51 596.3 0 51 149 123 596.3 0 123 150 34 596.3 0 34 151 34 596.3 0 34 152 62 596.3 0 62 153 54 596.3 0 54 154 51 596.3 0 51 155 34 596.3 0 34 156 30 596.3 0 30 157 25 596.3 0 25 158 32 596.3 0 32 159 31 596.3 0 31 160 31 596.3 0 31 161 28 596.3 0 28 162 52 596.3 0 52 163 63 596.3 0 63 164 52 596.3 0 52 165 76 596.3 0 76 166 114 596.3 0 114 167 1486 596.3 0 1486 168 953 596.3 0 953 169 2069 596.3 0 2069 170 85 596.3 0 85 171 189 596.3 0 189 172 133 596.3 0 133 173 307 596.3 0 307 174 32 596.3 0 32 175 35 596.3 0 35 176 44 596.3 0 44 177 42 596.3 0 42 178 38 596.3 0 38 179 30 596.3 0 30 180 94 596.3 0 94 181 308 596.3 0 308 182 433 596.3 0 433 183 68 596.3 0 68 184 94 596.3 0 94 185 134 596.3 0 134 186 155 596.3 0 155 187 144 596.3 0 144 188 78 596.3 0 78 189 92 596.3 0 92 190 84 596.3 0 84 191 72 596.3 0 72 192 41 596.3 0 41 193 44 596.3 0 44 194 42 596.3 0 42 195 52 596.3 0 52 196 49 596.3 0 49 197 40 596.3 0 40 198 95 596.3 0 95 199 171 596.3 0 171 200 717 596.3 0 717 201 425 596.3 0 425 202 347 596.3 0 347 203 242 596.3 0 242 204 41 596.3 0 41 205 83 596.3 0 83 206 112 596.3 0 112 207 121 596.3 0 121 208 51 596.3 0 51 209 33 596.3 0 33 210 63 596.3 0 63 211 83 596.3 0 83 212 52 596.3 0 52 213 75 596.3 0 75 214 97 596.3 0 97 215 305 596.3 0 305 216 230 596.3 0 230 217 110 596.3 0 110 218 111 596.3 0 111 219 135 596.3 0 135 220 138 596.3 0 138 221 96 596.3 0 96 222 91 596.3 0 91 223 126 596.3 0 126 224 195 596.3 0 195 225 255 596.3 0 255 226 392 596.3 0 392 227 833 596.3 0 833 228 10502 596.3 0 10502 229 4278 596.3 0 4278 230 6990 596.3 0 6990 231 321 596.3 0 321 232 93 596.3 0 93 233 50 596.3 0 50 234 51 596.3 0 51 235 62 596.3 0 62 236 67 596.3 0 67 237 55 596.3 0 55 238 85 596.3 0 85 239 78 596.3 0 78 240 94 596.3 0 94 241 56 596.3 0 56 242 58 596.3 0 58 243 110 596.3 0 110 244 154 596.3 0 154 245 154 596.3 0 154 246 204 596.3 0 204 247 275 596.3 0 275 248 251 596.3 0 251 249 184 596.3 0 184 250 143 596.3 0 143 251 101 596.3 0 101 252 78 596.3 0 78 253 56 596.3 0 56 254 76 596.3 0 76 255 186 596.3 0 186 256 691 596.3 0 691 257 729 596.3 0 729 258 778 596.3 0 778 259 136 596.3 0 136 260 157 596.3 0 157 261 460 596.3 0 460 262 1340 596.3 0 1340 263 1608 596.3 0 1608 264 736 596.3 0 736 265 362 596.3 0 362 266 140 596.3 0 140 267 176 596.3 0 176 268 981 596.3 0 981 269 939 596.3 0 939 270 724 596.3 0 724 271 178 596.3 0 178 272 81 596.3 0 81 273 62 596.3 0 62 274 134 596.3 0 134 275 162 596.3 0 162 276 137 596.3 0 137 277 112 596.3 0 112 278 98 596.3 0 98 279 73 596.3 0 73 280 111 596.3 0 111 281 180 596.3 0 180 282 210 596.3 0 210 283 285 596.3 0 285 284 79 596.3 0 79 285 73 596.3 0 73 286 109 596.3 0 109 287 261 596.3 0 261 288 203 596.3 0 203 289 158 596.3 0 158 290 77 596.3 0 77 291 75 596.3 0 75 292 210 596.3 0 210 293 345 596.3 0 345 294 519 596.3 0 519 295 152 596.3 0 152 296 101 596.3 0 101 297 67 596.3 0 67 298 70 596.3 0 70 299 121 596.3 0 121 300 438 596.3 0 438 301 530 596.3 0 530 Finished in 5.04 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 102,514 Reads with adapters: 28,110 (27.4%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 28,081 (27.4%) Total basepairs processed: 26,177,780 bp Total written (filtered): 7,635,308 bp (29.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28110 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 3 2 18 20 0.0 3 0 0 13 4 3 19 303 0.0 3 0 268 17 11 7 20 27466 0.0 4 25917 1254 227 50 18 21 79 0.0 4 25 49 1 3 1 22 17 0.0 4 7 2 5 2 1 23 147 0.0 4 36 0 1 84 26 24 3 0.0 4 1 0 0 1 1 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 34 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 42 5 0.0 4 0 0 0 0 5 44 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 1 75 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 23 0.0 4 0 0 0 0 23 128 1 0.0 4 0 1 139 3 0.0 4 0 0 0 0 3 142 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 2 6 229 5 0.0 4 0 0 0 0 5 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 28,081 Reads with adapters: 6,424 (22.9%) Reads written (passing filters): 28,081 (100.0%) Total basepairs processed: 7,635,308 bp Total written (filtered): 7,559,458 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6424 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 9.1% G: 86.1% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 438.8 0 15 4 3 109.7 0 3 7 1 1.7 0 1 9 780 0.1 0 80 700 10 4175 0.0 1 292 3883 11 1360 0.0 1 51 1309 12 12 0.0 1 0 12 13 16 0.0 1 1 15 14 4 0.0 1 0 4 15 1 0.0 1 0 1 18 1 0.0 1 1 35 5 0.0 1 3 2 36 1 0.0 1 1 150 1 0.0 1 1 152 1 0.0 1 0 1 166 1 0.0 1 1 208 3 0.0 1 2 1 223 28 0.0 1 24 4 224 2 0.0 1 2 226 2 0.0 1 2 264 12 0.0 1 11 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.