This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.63 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 148,965 (6.1%) Reads that were too short: 47,003 (1.9%) Reads written (passing filters): 101,962 (4.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 26,353,696 bp (3.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 148965 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 457 596.3 0 457 7 29313 596.3 0 29313 8 404 596.3 0 404 9 36425 596.3 0 36425 10 39 596.3 0 39 11 9 596.3 0 9 12 20 596.3 0 20 13 8 596.3 0 8 14 8 596.3 0 8 15 8 596.3 0 8 16 12 596.3 0 12 17 8 596.3 0 8 18 5 596.3 0 5 19 6 596.3 0 6 20 6 596.3 0 6 21 10 596.3 0 10 22 13 596.3 0 13 23 8 596.3 0 8 24 9 596.3 0 9 25 5 596.3 0 5 26 9 596.3 0 9 27 5 596.3 0 5 28 11 596.3 0 11 29 13 596.3 0 13 30 10 596.3 0 10 31 9 596.3 0 9 32 7 596.3 0 7 33 15 596.3 0 15 34 9 596.3 0 9 35 10 596.3 0 10 36 16 596.3 0 16 37 34 596.3 0 34 38 15 596.3 0 15 39 8 596.3 0 8 40 9 596.3 0 9 41 7 596.3 0 7 42 9 596.3 0 9 43 5 596.3 0 5 44 10 596.3 0 10 45 15 596.3 0 15 46 16 596.3 0 16 47 16 596.3 0 16 48 13 596.3 0 13 49 17 596.3 0 17 50 10 596.3 0 10 51 9 596.3 0 9 52 17 596.3 0 17 53 10 596.3 0 10 54 19 596.3 0 19 55 72 596.3 0 72 56 27 596.3 0 27 57 20 596.3 0 20 58 16 596.3 0 16 59 13 596.3 0 13 60 18 596.3 0 18 61 20 596.3 0 20 62 28 596.3 0 28 63 16 596.3 0 16 64 32 596.3 0 32 65 108 596.3 0 108 66 21 596.3 0 21 67 15 596.3 0 15 68 20 596.3 0 20 69 87 596.3 0 87 70 52 596.3 0 52 71 33 596.3 0 33 72 41 596.3 0 41 73 25 596.3 0 25 74 108 596.3 0 108 75 113 596.3 0 113 76 132 596.3 0 132 77 94 596.3 0 94 78 146 596.3 0 146 79 133 596.3 0 133 80 204 596.3 0 204 81 314 596.3 0 314 82 3429 596.3 0 3429 83 2495 596.3 0 2495 84 15886 596.3 0 15886 85 67 596.3 0 67 86 34 596.3 0 34 87 15 596.3 0 15 88 13 596.3 0 13 89 16 596.3 0 16 90 11 596.3 0 11 91 15 596.3 0 15 92 21 596.3 0 21 93 18 596.3 0 18 94 20 596.3 0 20 95 19 596.3 0 19 96 8 596.3 0 8 97 15 596.3 0 15 98 13 596.3 0 13 99 17 596.3 0 17 100 18 596.3 0 18 101 10 596.3 0 10 102 33 596.3 0 33 103 38 596.3 0 38 104 33 596.3 0 33 105 23 596.3 0 23 106 14 596.3 0 14 107 12 596.3 0 12 108 17 596.3 0 17 109 13 596.3 0 13 110 19 596.3 0 19 111 19 596.3 0 19 112 26 596.3 0 26 113 27 596.3 0 27 114 31 596.3 0 31 115 28 596.3 0 28 116 22 596.3 0 22 117 24 596.3 0 24 118 20 596.3 0 20 119 15 596.3 0 15 120 32 596.3 0 32 121 43 596.3 0 43 122 51 596.3 0 51 123 155 596.3 0 155 124 150 596.3 0 150 125 63 596.3 0 63 126 27 596.3 0 27 127 35 596.3 0 35 128 115 596.3 0 115 129 15 596.3 0 15 130 20 596.3 0 20 131 12 596.3 0 12 132 12 596.3 0 12 133 34 596.3 0 34 134 34 596.3 0 34 135 32 596.3 0 32 136 68 596.3 0 68 137 43 596.3 0 43 138 73 596.3 0 73 139 66 596.3 0 66 140 312 596.3 0 312 141 46 596.3 0 46 142 31 596.3 0 31 143 27 596.3 0 27 144 32 596.3 0 32 145 26 596.3 0 26 146 35 596.3 0 35 147 33 596.3 0 33 148 35 596.3 0 35 149 83 596.3 0 83 150 37 596.3 0 37 151 32 596.3 0 32 152 35 596.3 0 35 153 24 596.3 0 24 154 26 596.3 0 26 155 49 596.3 0 49 156 32 596.3 0 32 157 38 596.3 0 38 158 33 596.3 0 33 159 34 596.3 0 34 160 32 596.3 0 32 161 37 596.3 0 37 162 53 596.3 0 53 163 69 596.3 0 69 164 45 596.3 0 45 165 67 596.3 0 67 166 139 596.3 0 139 167 1629 596.3 0 1629 168 924 596.3 0 924 169 2080 596.3 0 2080 170 55 596.3 0 55 171 133 596.3 0 133 172 95 596.3 0 95 173 267 596.3 0 267 174 41 596.3 0 41 175 26 596.3 0 26 176 45 596.3 0 45 177 46 596.3 0 46 178 32 596.3 0 32 179 43 596.3 0 43 180 57 596.3 0 57 181 135 596.3 0 135 182 160 596.3 0 160 183 79 596.3 0 79 184 72 596.3 0 72 185 85 596.3 0 85 186 89 596.3 0 89 187 87 596.3 0 87 188 65 596.3 0 65 189 92 596.3 0 92 190 86 596.3 0 86 191 79 596.3 0 79 192 56 596.3 0 56 193 60 596.3 0 60 194 53 596.3 0 53 195 58 596.3 0 58 196 57 596.3 0 57 197 67 596.3 0 67 198 84 596.3 0 84 199 92 596.3 0 92 200 442 596.3 0 442 201 381 596.3 0 381 202 315 596.3 0 315 203 195 596.3 0 195 204 52 596.3 0 52 205 61 596.3 0 61 206 72 596.3 0 72 207 89 596.3 0 89 208 52 596.3 0 52 209 52 596.3 0 52 210 82 596.3 0 82 211 156 596.3 0 156 212 79 596.3 0 79 213 99 596.3 0 99 214 90 596.3 0 90 215 248 596.3 0 248 216 279 596.3 0 279 217 90 596.3 0 90 218 90 596.3 0 90 219 111 596.3 0 111 220 103 596.3 0 103 221 109 596.3 0 109 222 92 596.3 0 92 223 130 596.3 0 130 224 182 596.3 0 182 225 223 596.3 0 223 226 319 596.3 0 319 227 1023 596.3 0 1023 228 15780 596.3 0 15780 229 4188 596.3 0 4188 230 8177 596.3 0 8177 231 274 596.3 0 274 232 73 596.3 0 73 233 67 596.3 0 67 234 62 596.3 0 62 235 57 596.3 0 57 236 58 596.3 0 58 237 67 596.3 0 67 238 61 596.3 0 61 239 78 596.3 0 78 240 83 596.3 0 83 241 62 596.3 0 62 242 64 596.3 0 64 243 80 596.3 0 80 244 148 596.3 0 148 245 125 596.3 0 125 246 91 596.3 0 91 247 107 596.3 0 107 248 123 596.3 0 123 249 99 596.3 0 99 250 92 596.3 0 92 251 101 596.3 0 101 252 67 596.3 0 67 253 74 596.3 0 74 254 109 596.3 0 109 255 199 596.3 0 199 256 703 596.3 0 703 257 692 596.3 0 692 258 664 596.3 0 664 259 135 596.3 0 135 260 114 596.3 0 114 261 391 596.3 0 391 262 1043 596.3 0 1043 263 1484 596.3 0 1484 264 479 596.3 0 479 265 250 596.3 0 250 266 140 596.3 0 140 267 131 596.3 0 131 268 1058 596.3 0 1058 269 749 596.3 0 749 270 533 596.3 0 533 271 108 596.3 0 108 272 74 596.3 0 74 273 66 596.3 0 66 274 89 596.3 0 89 275 105 596.3 0 105 276 116 596.3 0 116 277 106 596.3 0 106 278 102 596.3 0 102 279 60 596.3 0 60 280 85 596.3 0 85 281 104 596.3 0 104 282 135 596.3 0 135 283 138 596.3 0 138 284 75 596.3 0 75 285 85 596.3 0 85 286 116 596.3 0 116 287 295 596.3 0 295 288 124 596.3 0 124 289 127 596.3 0 127 290 73 596.3 0 73 291 66 596.3 0 66 292 85 596.3 0 85 293 136 596.3 0 136 294 218 596.3 0 218 295 90 596.3 0 90 296 74 596.3 0 74 297 62 596.3 0 62 298 62 596.3 0 62 299 87 596.3 0 87 300 167 596.3 0 167 301 248 596.3 0 248 Finished in 4.57 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 101,962 Reads with adapters: 29,208 (28.6%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 29,174 (28.6%) Total basepairs processed: 26,353,696 bp Total written (filtered): 8,077,674 bp (30.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 29208 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 1 16 245 0.0 3 0 241 4 17 28788 0.0 3 28221 545 19 3 18 59 0.0 3 8 49 1 1 19 16 0.0 3 0 0 11 5 20 38 0.0 3 0 0 0 38 24 4 0.0 3 0 0 1 3 31 3 0.0 3 0 0 0 3 46 3 0.0 3 0 0 0 3 50 3 0.0 3 0 0 0 3 60 2 0.0 3 0 0 1 1 71 4 0.0 3 0 0 0 4 74 1 0.0 3 0 0 1 95 2 0.0 3 0 0 0 2 96 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 98 5 0.0 3 0 0 0 5 107 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 175 1 0.0 3 1 183 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 29,174 Reads with adapters: 83 (0.3%) Reads written (passing filters): 29,174 (100.0%) Total basepairs processed: 8,077,674 bp Total written (filtered): 8,077,418 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 83 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.5% C: 59.0% G: 12.0% T: 8.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 79 455.8 0 79 4 2 114.0 0 2 5 1 28.5 0 1 6 1 7.1 0 1