This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.52 s (28 us/read; 2.14 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 1,320,770 (54.1%) Reads that were too short: 34,785 (1.4%) Reads written (passing filters): 1,285,985 (52.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 350,840,776 bp (47.7%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1320770 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 986 596.3 0 986 7 60056 596.3 0 60056 8 1910 596.3 0 1910 9 46778 596.3 0 46778 10 915 596.3 0 915 11 1278 596.3 0 1278 12 83426 596.3 0 83426 13 3652 596.3 0 3652 14 615 596.3 0 615 15 1346 596.3 0 1346 16 439 596.3 0 439 17 952 596.3 0 952 18 3622 596.3 0 3622 19 1000 596.3 0 1000 20 1184 596.3 0 1184 21 413 596.3 0 413 22 643 596.3 0 643 23 1590 596.3 0 1590 24 1889 596.3 0 1889 25 3312 596.3 0 3312 26 5564 596.3 0 5564 27 5775 596.3 0 5775 28 11615 596.3 0 11615 29 23831 596.3 0 23831 30 353367 596.3 0 353367 31 210815 596.3 0 210815 32 447390 596.3 0 447390 33 1484 596.3 0 1484 34 146 596.3 0 146 35 166 596.3 0 166 36 340 596.3 0 340 37 130 596.3 0 130 38 14 596.3 0 14 39 152 596.3 0 152 40 15 596.3 0 15 41 19 596.3 0 19 42 14 596.3 0 14 43 26 596.3 0 26 44 26 596.3 0 26 45 35 596.3 0 35 46 13 596.3 0 13 47 28 596.3 0 28 48 14 596.3 0 14 49 22 596.3 0 22 50 11 596.3 0 11 51 14 596.3 0 14 52 11 596.3 0 11 53 18 596.3 0 18 54 125 596.3 0 125 55 225 596.3 0 225 56 249 596.3 0 249 57 11 596.3 0 11 58 18 596.3 0 18 59 15 596.3 0 15 60 18 596.3 0 18 61 8 596.3 0 8 62 11 596.3 0 11 63 17 596.3 0 17 64 17 596.3 0 17 65 13 596.3 0 13 66 15 596.3 0 15 67 12 596.3 0 12 68 15 596.3 0 15 69 26 596.3 0 26 70 23 596.3 0 23 71 19 596.3 0 19 72 15 596.3 0 15 73 13 596.3 0 13 74 15 596.3 0 15 75 14 596.3 0 14 76 15 596.3 0 15 77 7 596.3 0 7 78 14 596.3 0 14 79 19 596.3 0 19 80 35 596.3 0 35 81 48 596.3 0 48 82 43 596.3 0 43 83 58 596.3 0 58 84 31 596.3 0 31 85 41 596.3 0 41 86 41 596.3 0 41 87 62 596.3 0 62 88 147 596.3 0 147 89 58 596.3 0 58 90 32 596.3 0 32 91 20 596.3 0 20 92 32 596.3 0 32 93 32 596.3 0 32 94 25 596.3 0 25 95 22 596.3 0 22 96 26 596.3 0 26 97 30 596.3 0 30 98 27 596.3 0 27 99 19 596.3 0 19 100 12 596.3 0 12 101 33 596.3 0 33 102 121 596.3 0 121 103 135 596.3 0 135 104 311 596.3 0 311 105 522 596.3 0 522 106 773 596.3 0 773 107 485 596.3 0 485 108 17 596.3 0 17 109 26 596.3 0 26 110 15 596.3 0 15 111 28 596.3 0 28 112 28 596.3 0 28 113 35 596.3 0 35 114 205 596.3 0 205 115 14 596.3 0 14 116 21 596.3 0 21 117 31 596.3 0 31 118 20 596.3 0 20 119 25 596.3 0 25 120 29 596.3 0 29 121 16 596.3 0 16 122 32 596.3 0 32 123 53 596.3 0 53 124 218 596.3 0 218 125 364 596.3 0 364 126 167 596.3 0 167 127 38 596.3 0 38 128 39 596.3 0 39 129 35 596.3 0 35 130 17 596.3 0 17 131 20 596.3 0 20 132 16 596.3 0 16 133 22 596.3 0 22 134 24 596.3 0 24 135 12 596.3 0 12 136 24 596.3 0 24 137 93 596.3 0 93 138 103 596.3 0 103 139 132 596.3 0 132 140 42 596.3 0 42 141 160 596.3 0 160 142 56 596.3 0 56 143 74 596.3 0 74 144 30 596.3 0 30 145 27 596.3 0 27 146 30 596.3 0 30 147 26 596.3 0 26 148 53 596.3 0 53 149 67 596.3 0 67 150 45 596.3 0 45 151 42 596.3 0 42 152 22 596.3 0 22 153 24 596.3 0 24 154 20 596.3 0 20 155 28 596.3 0 28 156 27 596.3 0 27 157 34 596.3 0 34 158 23 596.3 0 23 159 129 596.3 0 129 160 80 596.3 0 80 161 251 596.3 0 251 162 49 596.3 0 49 163 44 596.3 0 44 164 34 596.3 0 34 165 41 596.3 0 41 166 70 596.3 0 70 167 49 596.3 0 49 168 248 596.3 0 248 169 26 596.3 0 26 170 22 596.3 0 22 171 35 596.3 0 35 172 26 596.3 0 26 173 25 596.3 0 25 174 19 596.3 0 19 175 33 596.3 0 33 176 27 596.3 0 27 177 39 596.3 0 39 178 22 596.3 0 22 179 14 596.3 0 14 180 22 596.3 0 22 181 27 596.3 0 27 182 40 596.3 0 40 183 57 596.3 0 57 184 42 596.3 0 42 185 34 596.3 0 34 186 32 596.3 0 32 187 40 596.3 0 40 188 34 596.3 0 34 189 33 596.3 0 33 190 26 596.3 0 26 191 25 596.3 0 25 192 34 596.3 0 34 193 42 596.3 0 42 194 19 596.3 0 19 195 23 596.3 0 23 196 24 596.3 0 24 197 18 596.3 0 18 198 24 596.3 0 24 199 41 596.3 0 41 200 46 596.3 0 46 201 39 596.3 0 39 202 30 596.3 0 30 203 29 596.3 0 29 204 43 596.3 0 43 205 113 596.3 0 113 206 148 596.3 0 148 207 58 596.3 0 58 208 20 596.3 0 20 209 24 596.3 0 24 210 25 596.3 0 25 211 32 596.3 0 32 212 32 596.3 0 32 213 40 596.3 0 40 214 40 596.3 0 40 215 48 596.3 0 48 216 38 596.3 0 38 217 40 596.3 0 40 218 35 596.3 0 35 219 43 596.3 0 43 220 51 596.3 0 51 221 38 596.3 0 38 222 26 596.3 0 26 223 19 596.3 0 19 224 26 596.3 0 26 225 33 596.3 0 33 226 94 596.3 0 94 227 130 596.3 0 130 228 145 596.3 0 145 229 39 596.3 0 39 230 45 596.3 0 45 231 54 596.3 0 54 232 79 596.3 0 79 233 56 596.3 0 56 234 35 596.3 0 35 235 24 596.3 0 24 236 60 596.3 0 60 237 28 596.3 0 28 238 28 596.3 0 28 239 39 596.3 0 39 240 45 596.3 0 45 241 65 596.3 0 65 242 49 596.3 0 49 243 41 596.3 0 41 244 59 596.3 0 59 245 60 596.3 0 60 246 102 596.3 0 102 247 207 596.3 0 207 248 75 596.3 0 75 249 108 596.3 0 108 250 190 596.3 0 190 251 101 596.3 0 101 252 107 596.3 0 107 253 157 596.3 0 157 254 1145 596.3 0 1145 255 2153 596.3 0 2153 256 1307 596.3 0 1307 257 1000 596.3 0 1000 258 287 596.3 0 287 259 290 596.3 0 290 260 3874 596.3 0 3874 261 5786 596.3 0 5786 262 6916 596.3 0 6916 263 142 596.3 0 142 264 51 596.3 0 51 265 81 596.3 0 81 266 54 596.3 0 54 267 68 596.3 0 68 268 75 596.3 0 75 269 106 596.3 0 106 270 88 596.3 0 88 271 76 596.3 0 76 272 259 596.3 0 259 273 360 596.3 0 360 274 716 596.3 0 716 275 1492 596.3 0 1492 276 1136 596.3 0 1136 277 1509 596.3 0 1509 278 609 596.3 0 609 279 427 596.3 0 427 280 191 596.3 0 191 281 363 596.3 0 363 282 266 596.3 0 266 283 125 596.3 0 125 284 23 596.3 0 23 285 34 596.3 0 34 286 20 596.3 0 20 287 23 596.3 0 23 288 42 596.3 0 42 289 46 596.3 0 46 290 41 596.3 0 41 291 37 596.3 0 37 292 30 596.3 0 30 293 40 596.3 0 40 294 49 596.3 0 49 295 49 596.3 0 49 296 26 596.3 0 26 297 19 596.3 0 19 298 18 596.3 0 18 299 27 596.3 0 27 300 25 596.3 0 25 301 31 596.3 0 31 Finished in 53.18 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 1,285,985 Reads with adapters: 49,497 (3.8%) Reads that were too short: 408 (0.0%) Reads written (passing filters): 49,089 (3.8%) Total basepairs processed: 350,840,776 bp Total written (filtered): 13,397,136 bp (3.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 49497 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 13 0.0 3 0 0 10 3 16 290 0.0 3 0 277 9 4 17 47857 0.0 3 46839 983 31 4 18 114 0.0 3 11 96 5 2 19 35 0.0 3 1 0 25 9 20 33 0.0 3 0 0 0 33 21 1 0.0 3 0 1 22 1 0.0 3 1 23 2 0.0 3 0 1 0 1 24 2 0.0 3 1 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 1 32 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 45 5 0.0 3 0 0 0 5 46 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 7 0.0 3 0 0 0 7 68 5 0.0 3 0 0 0 5 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 1 1 102 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 2 0.0 3 1 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 52 0.0 3 0 0 0 52 113 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 104 0.0 3 0 0 0 104 127 11 0.0 3 0 0 0 11 130 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 137 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 141 15 0.0 3 0 0 0 15 143 1 0.0 3 0 0 0 1 148 1 0.0 3 1 150 5 0.0 3 0 0 0 5 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 13 0.0 3 0 0 0 13 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 6 0.0 3 0 0 0 6 161 74 0.0 3 0 0 1 73 162 135 0.0 3 0 0 0 135 163 53 0.0 3 0 0 0 53 164 18 0.0 3 0 0 1 17 165 17 0.0 3 0 0 1 16 166 6 0.0 3 0 0 0 6 168 1 0.0 3 0 0 0 1 169 19 0.0 3 0 0 1 18 170 1 0.0 3 0 0 0 1 171 19 0.0 3 0 0 0 19 172 3 0.0 3 0 0 0 3 173 3 0.0 3 0 0 0 3 174 1 0.0 3 0 0 0 1 176 2 0.0 3 0 1 0 1 177 2 0.0 3 0 0 0 2 179 3 0.0 3 0 0 0 3 180 4 0.0 3 0 0 0 4 181 80 0.0 3 0 0 0 80 182 42 0.0 3 0 0 0 42 183 8 0.0 3 0 0 1 7 184 7 0.0 3 0 0 0 7 185 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 188 3 0.0 3 0 0 0 3 189 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 1 3 193 3 0.0 3 0 0 0 3 194 17 0.0 3 0 0 0 17 195 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 198 32 0.0 3 0 0 0 32 199 3 0.0 3 0 0 0 3 203 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 213 3 0.0 3 0 0 0 3 214 2 0.0 3 0 0 0 2 215 4 0.0 3 0 0 0 4 216 10 0.0 3 0 0 0 10 220 1 0.0 3 0 0 0 1 227 1 0.0 3 1 234 1 0.0 3 1 240 1 0.0 3 0 0 0 1 241 3 0.0 3 0 0 0 3 242 2 0.0 3 0 0 0 2 247 5 0.0 3 0 0 0 5 248 2 0.0 3 0 0 0 2 255 5 0.0 3 0 0 0 5 256 35 0.0 3 0 0 0 35 257 46 0.0 3 0 0 0 46 258 36 0.0 3 0 0 0 36 259 43 0.0 3 0 0 0 43 260 9 0.0 3 0 0 0 9 261 2 0.0 3 0 0 0 2 262 2 0.0 3 0 0 0 2 263 3 0.0 3 0 0 1 2 265 3 0.0 3 0 0 0 3 266 8 0.0 3 0 0 0 8 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 273 6 0.0 3 0 0 0 6 274 7 0.0 3 0 0 0 7 275 10 0.0 3 0 0 0 10 276 2 0.0 3 0 0 0 2 277 18 0.0 3 0 0 1 17 278 4 0.0 3 0 0 0 4 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 6 0.0 3 0 0 0 6 283 9 0.0 3 0 0 0 9 284 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.72 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 49,089 Reads with adapters: 1,487 (3.0%) Reads written (passing filters): 49,089 (100.0%) Total basepairs processed: 13,397,136 bp Total written (filtered): 13,392,626 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1487 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.2% C: 7.5% G: 15.5% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1438 767.0 0 1438 4 49 191.8 0 49