This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.22 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 350,507 (14.4%) Reads that were too short: 21,723 (0.9%) Reads written (passing filters): 328,784 (13.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 90,852,215 bp (12.4%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 350507 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2424 596.3 0 2424 7 2860 596.3 0 2860 8 138563 596.3 0 138563 9 124993 596.3 0 124993 10 160 596.3 0 160 11 29 596.3 0 29 12 24 596.3 0 24 13 28 596.3 0 28 14 28 596.3 0 28 15 25 596.3 0 25 16 30 596.3 0 30 17 21 596.3 0 21 18 22 596.3 0 22 19 34 596.3 0 34 20 21 596.3 0 21 21 23 596.3 0 23 22 17 596.3 0 17 23 24 596.3 0 24 24 65 596.3 0 65 25 32 596.3 0 32 26 17 596.3 0 17 27 9 596.3 0 9 28 20 596.3 0 20 29 18 596.3 0 18 30 17 596.3 0 17 31 27 596.3 0 27 32 38 596.3 0 38 33 29 596.3 0 29 34 16 596.3 0 16 35 41 596.3 0 41 36 22 596.3 0 22 37 25 596.3 0 25 38 30 596.3 0 30 39 31 596.3 0 31 40 22 596.3 0 22 41 29 596.3 0 29 42 111 596.3 0 111 43 22 596.3 0 22 44 29 596.3 0 29 45 24 596.3 0 24 46 19 596.3 0 19 47 16 596.3 0 16 48 12 596.3 0 12 49 33 596.3 0 33 50 34 596.3 0 34 51 58 596.3 0 58 52 126 596.3 0 126 53 51 596.3 0 51 54 786 596.3 0 786 55 101 596.3 0 101 56 50 596.3 0 50 57 124 596.3 0 124 58 52 596.3 0 52 59 65 596.3 0 65 60 42 596.3 0 42 61 47 596.3 0 47 62 77 596.3 0 77 63 100 596.3 0 100 64 150 596.3 0 150 65 241 596.3 0 241 66 254 596.3 0 254 67 486 596.3 0 486 68 1019 596.3 0 1019 69 12282 596.3 0 12282 70 11545 596.3 0 11545 71 9033 596.3 0 9033 72 58 596.3 0 58 73 22 596.3 0 22 74 26 596.3 0 26 75 37 596.3 0 37 76 51 596.3 0 51 77 74 596.3 0 74 78 56 596.3 0 56 79 38 596.3 0 38 80 298 596.3 0 298 81 131 596.3 0 131 82 217 596.3 0 217 83 38 596.3 0 38 84 21 596.3 0 21 85 22 596.3 0 22 86 45 596.3 0 45 87 58 596.3 0 58 88 33 596.3 0 33 89 42 596.3 0 42 90 60 596.3 0 60 91 61 596.3 0 61 92 28 596.3 0 28 93 49 596.3 0 49 94 43 596.3 0 43 95 36 596.3 0 36 96 99 596.3 0 99 97 81 596.3 0 81 98 40 596.3 0 40 99 63 596.3 0 63 100 518 596.3 0 518 101 807 596.3 0 807 102 1453 596.3 0 1453 103 118 596.3 0 118 104 39 596.3 0 39 105 34 596.3 0 34 106 26 596.3 0 26 107 46 596.3 0 46 108 39 596.3 0 39 109 47 596.3 0 47 110 46 596.3 0 46 111 59 596.3 0 59 112 74 596.3 0 74 113 74 596.3 0 74 114 53 596.3 0 53 115 51 596.3 0 51 116 50 596.3 0 50 117 70 596.3 0 70 118 72 596.3 0 72 119 77 596.3 0 77 120 36 596.3 0 36 121 45 596.3 0 45 122 74 596.3 0 74 123 70 596.3 0 70 124 85 596.3 0 85 125 57 596.3 0 57 126 57 596.3 0 57 127 42 596.3 0 42 128 46 596.3 0 46 129 35 596.3 0 35 130 70 596.3 0 70 131 123 596.3 0 123 132 535 596.3 0 535 133 637 596.3 0 637 134 574 596.3 0 574 135 71 596.3 0 71 136 320 596.3 0 320 137 304 596.3 0 304 138 280 596.3 0 280 139 117 596.3 0 117 140 81 596.3 0 81 141 61 596.3 0 61 142 54 596.3 0 54 143 75 596.3 0 75 144 98 596.3 0 98 145 62 596.3 0 62 146 61 596.3 0 61 147 68 596.3 0 68 148 81 596.3 0 81 149 58 596.3 0 58 150 72 596.3 0 72 151 75 596.3 0 75 152 124 596.3 0 124 153 104 596.3 0 104 154 143 596.3 0 143 155 89 596.3 0 89 156 79 596.3 0 79 157 130 596.3 0 130 158 199 596.3 0 199 159 201 596.3 0 201 160 277 596.3 0 277 161 394 596.3 0 394 162 91 596.3 0 91 163 127 596.3 0 127 164 90 596.3 0 90 165 60 596.3 0 60 166 149 596.3 0 149 167 83 596.3 0 83 168 115 596.3 0 115 169 170 596.3 0 170 170 595 596.3 0 595 171 97 596.3 0 97 172 146 596.3 0 146 173 54 596.3 0 54 174 80 596.3 0 80 175 85 596.3 0 85 176 297 596.3 0 297 177 491 596.3 0 491 178 954 596.3 0 954 179 1183 596.3 0 1183 180 351 596.3 0 351 181 144 596.3 0 144 182 493 596.3 0 493 183 730 596.3 0 730 184 1046 596.3 0 1046 185 356 596.3 0 356 186 155 596.3 0 155 187 145 596.3 0 145 188 131 596.3 0 131 189 163 596.3 0 163 190 148 596.3 0 148 191 117 596.3 0 117 192 104 596.3 0 104 193 70 596.3 0 70 194 74 596.3 0 74 195 71 596.3 0 71 196 130 596.3 0 130 197 212 596.3 0 212 198 296 596.3 0 296 199 245 596.3 0 245 200 113 596.3 0 113 201 76 596.3 0 76 202 483 596.3 0 483 203 739 596.3 0 739 204 745 596.3 0 745 205 601 596.3 0 601 206 76 596.3 0 76 207 65 596.3 0 65 208 64 596.3 0 64 209 78 596.3 0 78 210 87 596.3 0 87 211 177 596.3 0 177 212 127 596.3 0 127 213 102 596.3 0 102 214 91 596.3 0 91 215 71 596.3 0 71 216 90 596.3 0 90 217 114 596.3 0 114 218 150 596.3 0 150 219 144 596.3 0 144 220 77 596.3 0 77 221 90 596.3 0 90 222 282 596.3 0 282 223 697 596.3 0 697 224 639 596.3 0 639 225 440 596.3 0 440 226 148 596.3 0 148 227 141 596.3 0 141 228 150 596.3 0 150 229 144 596.3 0 144 230 84 596.3 0 84 231 63 596.3 0 63 232 78 596.3 0 78 233 77 596.3 0 77 234 80 596.3 0 80 235 78 596.3 0 78 236 66 596.3 0 66 237 98 596.3 0 98 238 77 596.3 0 77 239 102 596.3 0 102 240 55 596.3 0 55 241 99 596.3 0 99 242 321 596.3 0 321 243 482 596.3 0 482 244 597 596.3 0 597 245 363 596.3 0 363 246 113 596.3 0 113 247 73 596.3 0 73 248 51 596.3 0 51 249 55 596.3 0 55 250 66 596.3 0 66 251 131 596.3 0 131 252 165 596.3 0 165 253 212 596.3 0 212 254 104 596.3 0 104 255 336 596.3 0 336 256 284 596.3 0 284 257 306 596.3 0 306 258 137 596.3 0 137 259 150 596.3 0 150 260 106 596.3 0 106 261 118 596.3 0 118 262 141 596.3 0 141 263 139 596.3 0 139 264 205 596.3 0 205 265 540 596.3 0 540 266 342 596.3 0 342 267 306 596.3 0 306 268 125 596.3 0 125 269 374 596.3 0 374 270 499 596.3 0 499 271 879 596.3 0 879 272 402 596.3 0 402 273 435 596.3 0 435 274 247 596.3 0 247 275 142 596.3 0 142 276 201 596.3 0 201 277 313 596.3 0 313 278 359 596.3 0 359 279 309 596.3 0 309 280 98 596.3 0 98 281 88 596.3 0 88 282 89 596.3 0 89 283 165 596.3 0 165 284 169 596.3 0 169 285 204 596.3 0 204 286 182 596.3 0 182 287 126 596.3 0 126 288 155 596.3 0 155 289 88 596.3 0 88 290 87 596.3 0 87 291 255 596.3 0 255 292 476 596.3 0 476 293 306 596.3 0 306 294 230 596.3 0 230 295 117 596.3 0 117 296 77 596.3 0 77 297 145 596.3 0 145 298 118 596.3 0 118 299 156 596.3 0 156 300 198 596.3 0 198 301 297 596.3 0 297 Finished in 15.66 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 328,784 Reads with adapters: 131,354 (40.0%) Reads that were too short: 151 (0.0%) Reads written (passing filters): 131,203 (39.9%) Total basepairs processed: 90,852,215 bp Total written (filtered): 35,471,749 bp (39.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 131354 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 13 0.0 3 0 0 0 5 8 18 98 0.0 3 0 0 61 17 20 19 2162 0.0 3 0 1931 120 81 30 20 125998 0.0 4 118107 6280 1180 329 102 21 292 0.0 4 51 213 17 6 5 22 23 0.0 4 0 0 8 1 14 23 516 0.0 4 8 0 1 392 115 24 8 0.0 4 0 0 0 1 7 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 1 28 2 0.0 4 1 0 0 0 1 29 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 34 4 0.0 4 1 0 0 0 3 37 1 0.0 4 1 38 7 0.0 4 1 0 0 0 6 39 3 0.0 4 0 0 0 0 3 46 1 0.0 4 0 0 0 0 1 54 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 1 57 2 0.0 4 0 0 0 0 2 60 3 0.0 4 0 0 0 0 3 61 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 100 1 0.0 4 1 103 1 0.0 4 0 0 0 0 1 104 4 0.0 4 0 0 0 0 4 105 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 28 0.0 4 0 0 0 0 28 127 1965 0.0 4 0 0 0 0 1965 128 34 0.0 4 0 0 0 3 31 129 2 0.0 4 0 0 0 0 2 130 10 0.0 4 0 0 0 0 10 132 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 135 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 12 0.0 4 0 0 0 0 12 155 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 204 1 0.0 4 0 0 0 0 1 205 2 0.0 4 0 0 0 0 2 206 1 0.0 4 0 0 0 0 1 207 3 0.0 4 0 0 0 0 3 210 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 3 0.0 4 0 0 0 0 3 216 1 0.0 4 0 0 0 0 1 218 2 0.0 4 0 0 0 1 1 219 3 0.0 4 0 0 0 0 3 222 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 3 0.0 4 0 0 0 0 3 227 1 0.0 4 0 0 0 0 1 228 35 0.0 4 0 0 0 1 34 229 11 0.0 4 0 0 0 0 11 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 5 0.0 4 0 0 0 0 5 235 3 0.0 4 0 0 0 0 3 236 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 3 0.0 4 0 0 0 1 2 275 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 1 289 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.81 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 131,203 Reads with adapters: 29,111 (22.2%) Reads written (passing filters): 131,203 (100.0%) Total basepairs processed: 35,471,749 bp Total written (filtered): 35,116,765 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 29111 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 9.5% G: 85.4% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 98 2050.0 0 98 4 27 512.5 0 27 5 2 128.1 0 2 6 2 32.0 0 2 7 6 8.0 0 6 8 9 2.0 0 9 9 5849 0.5 0 507 5342 10 15179 0.1 1 1131 14048 11 7468 0.0 1 284 7184 12 89 0.0 1 2 87 13 63 0.0 1 0 63 14 8 0.0 1 0 8 15 2 0.0 1 0 2 18 4 0.0 1 0 4 21 1 0.0 1 1 41 1 0.0 1 1 68 2 0.0 1 2 72 2 0.0 1 2 134 1 0.0 1 1 150 18 0.0 1 13 5 151 1 0.0 1 1 152 1 0.0 1 1 177 1 0.0 1 1 189 1 0.0 1 0 1 199 1 0.0 1 1 208 26 0.0 1 19 7 209 2 0.0 1 1 1 223 210 0.0 1 191 19 224 8 0.0 1 7 1 225 2 0.0 1 2 226 1 0.0 1 0 1 227 1 0.0 1 1 228 3 0.0 1 3 230 1 0.0 1 1 257 1 0.0 1 1 260 20 0.0 1 20 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.