This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.27 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 352,392 (14.4%) Reads that were too short: 10,733 (0.4%) Reads written (passing filters): 341,659 (14.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 95,139,757 bp (12.9%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 352392 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2145 596.3 0 2145 7 2136 596.3 0 2136 8 129906 596.3 0 129906 9 145130 596.3 0 145130 10 159 596.3 0 159 11 33 596.3 0 33 12 32 596.3 0 32 13 23 596.3 0 23 14 13 596.3 0 13 15 24 596.3 0 24 16 31 596.3 0 31 17 28 596.3 0 28 18 23 596.3 0 23 19 36 596.3 0 36 20 24 596.3 0 24 21 27 596.3 0 27 22 21 596.3 0 21 23 20 596.3 0 20 24 16 596.3 0 16 25 23 596.3 0 23 26 27 596.3 0 27 27 27 596.3 0 27 28 27 596.3 0 27 29 28 596.3 0 28 30 32 596.3 0 32 31 38 596.3 0 38 32 47 596.3 0 47 33 48 596.3 0 48 34 35 596.3 0 35 35 33 596.3 0 33 36 29 596.3 0 29 37 32 596.3 0 32 38 23 596.3 0 23 39 33 596.3 0 33 40 21 596.3 0 21 41 49 596.3 0 49 42 145 596.3 0 145 43 37 596.3 0 37 44 35 596.3 0 35 45 28 596.3 0 28 46 37 596.3 0 37 47 20 596.3 0 20 48 24 596.3 0 24 49 27 596.3 0 27 50 46 596.3 0 46 51 77 596.3 0 77 52 141 596.3 0 141 53 46 596.3 0 46 54 80 596.3 0 80 55 44 596.3 0 44 56 78 596.3 0 78 57 186 596.3 0 186 58 69 596.3 0 69 59 74 596.3 0 74 60 48 596.3 0 48 61 46 596.3 0 46 62 79 596.3 0 79 63 119 596.3 0 119 64 151 596.3 0 151 65 269 596.3 0 269 66 286 596.3 0 286 67 581 596.3 0 581 68 1155 596.3 0 1155 69 16965 596.3 0 16965 70 14812 596.3 0 14812 71 11972 596.3 0 11972 72 59 596.3 0 59 73 25 596.3 0 25 74 29 596.3 0 29 75 25 596.3 0 25 76 34 596.3 0 34 77 35 596.3 0 35 78 32 596.3 0 32 79 37 596.3 0 37 80 199 596.3 0 199 81 109 596.3 0 109 82 175 596.3 0 175 83 39 596.3 0 39 84 35 596.3 0 35 85 29 596.3 0 29 86 42 596.3 0 42 87 48 596.3 0 48 88 40 596.3 0 40 89 39 596.3 0 39 90 46 596.3 0 46 91 49 596.3 0 49 92 43 596.3 0 43 93 46 596.3 0 46 94 53 596.3 0 53 95 53 596.3 0 53 96 44 596.3 0 44 97 57 596.3 0 57 98 42 596.3 0 42 99 44 596.3 0 44 100 150 596.3 0 150 101 249 596.3 0 249 102 441 596.3 0 441 103 71 596.3 0 71 104 52 596.3 0 52 105 48 596.3 0 48 106 33 596.3 0 33 107 41 596.3 0 41 108 37 596.3 0 37 109 46 596.3 0 46 110 57 596.3 0 57 111 56 596.3 0 56 112 39 596.3 0 39 113 45 596.3 0 45 114 41 596.3 0 41 115 43 596.3 0 43 116 40 596.3 0 40 117 51 596.3 0 51 118 71 596.3 0 71 119 52 596.3 0 52 120 41 596.3 0 41 121 47 596.3 0 47 122 42 596.3 0 42 123 80 596.3 0 80 124 58 596.3 0 58 125 42 596.3 0 42 126 48 596.3 0 48 127 46 596.3 0 46 128 30 596.3 0 30 129 47 596.3 0 47 130 52 596.3 0 52 131 104 596.3 0 104 132 346 596.3 0 346 133 593 596.3 0 593 134 421 596.3 0 421 135 68 596.3 0 68 136 149 596.3 0 149 137 183 596.3 0 183 138 199 596.3 0 199 139 85 596.3 0 85 140 64 596.3 0 64 141 44 596.3 0 44 142 64 596.3 0 64 143 52 596.3 0 52 144 60 596.3 0 60 145 62 596.3 0 62 146 54 596.3 0 54 147 47 596.3 0 47 148 64 596.3 0 64 149 56 596.3 0 56 150 70 596.3 0 70 151 62 596.3 0 62 152 98 596.3 0 98 153 67 596.3 0 67 154 104 596.3 0 104 155 75 596.3 0 75 156 61 596.3 0 61 157 81 596.3 0 81 158 100 596.3 0 100 159 131 596.3 0 131 160 238 596.3 0 238 161 388 596.3 0 388 162 65 596.3 0 65 163 87 596.3 0 87 164 67 596.3 0 67 165 38 596.3 0 38 166 157 596.3 0 157 167 65 596.3 0 65 168 80 596.3 0 80 169 96 596.3 0 96 170 704 596.3 0 704 171 57 596.3 0 57 172 148 596.3 0 148 173 67 596.3 0 67 174 62 596.3 0 62 175 72 596.3 0 72 176 194 596.3 0 194 177 223 596.3 0 223 178 543 596.3 0 543 179 590 596.3 0 590 180 230 596.3 0 230 181 79 596.3 0 79 182 154 596.3 0 154 183 186 596.3 0 186 184 306 596.3 0 306 185 120 596.3 0 120 186 68 596.3 0 68 187 119 596.3 0 119 188 83 596.3 0 83 189 105 596.3 0 105 190 72 596.3 0 72 191 61 596.3 0 61 192 63 596.3 0 63 193 72 596.3 0 72 194 56 596.3 0 56 195 63 596.3 0 63 196 67 596.3 0 67 197 101 596.3 0 101 198 136 596.3 0 136 199 122 596.3 0 122 200 82 596.3 0 82 201 49 596.3 0 49 202 149 596.3 0 149 203 287 596.3 0 287 204 219 596.3 0 219 205 142 596.3 0 142 206 51 596.3 0 51 207 47 596.3 0 47 208 54 596.3 0 54 209 66 596.3 0 66 210 69 596.3 0 69 211 90 596.3 0 90 212 82 596.3 0 82 213 86 596.3 0 86 214 56 596.3 0 56 215 47 596.3 0 47 216 54 596.3 0 54 217 64 596.3 0 64 218 74 596.3 0 74 219 64 596.3 0 64 220 46 596.3 0 46 221 61 596.3 0 61 222 113 596.3 0 113 223 235 596.3 0 235 224 213 596.3 0 213 225 155 596.3 0 155 226 105 596.3 0 105 227 96 596.3 0 96 228 133 596.3 0 133 229 102 596.3 0 102 230 68 596.3 0 68 231 41 596.3 0 41 232 58 596.3 0 58 233 58 596.3 0 58 234 69 596.3 0 69 235 51 596.3 0 51 236 56 596.3 0 56 237 72 596.3 0 72 238 70 596.3 0 70 239 69 596.3 0 69 240 60 596.3 0 60 241 47 596.3 0 47 242 115 596.3 0 115 243 158 596.3 0 158 244 182 596.3 0 182 245 127 596.3 0 127 246 77 596.3 0 77 247 51 596.3 0 51 248 52 596.3 0 52 249 57 596.3 0 57 250 87 596.3 0 87 251 75 596.3 0 75 252 103 596.3 0 103 253 104 596.3 0 104 254 56 596.3 0 56 255 137 596.3 0 137 256 109 596.3 0 109 257 124 596.3 0 124 258 62 596.3 0 62 259 69 596.3 0 69 260 56 596.3 0 56 261 64 596.3 0 64 262 70 596.3 0 70 263 95 596.3 0 95 264 85 596.3 0 85 265 265 596.3 0 265 266 128 596.3 0 128 267 161 596.3 0 161 268 55 596.3 0 55 269 245 596.3 0 245 270 319 596.3 0 319 271 486 596.3 0 486 272 162 596.3 0 162 273 174 596.3 0 174 274 141 596.3 0 141 275 83 596.3 0 83 276 103 596.3 0 103 277 152 596.3 0 152 278 161 596.3 0 161 279 152 596.3 0 152 280 70 596.3 0 70 281 76 596.3 0 76 282 63 596.3 0 63 283 102 596.3 0 102 284 106 596.3 0 106 285 109 596.3 0 109 286 109 596.3 0 109 287 79 596.3 0 79 288 90 596.3 0 90 289 55 596.3 0 55 290 59 596.3 0 59 291 125 596.3 0 125 292 212 596.3 0 212 293 123 596.3 0 123 294 141 596.3 0 141 295 71 596.3 0 71 296 60 596.3 0 60 297 82 596.3 0 82 298 83 596.3 0 83 299 98 596.3 0 98 300 136 596.3 0 136 301 103 596.3 0 103 Finished in 14.43 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 341,659 Reads with adapters: 129,615 (37.9%) Reads that were too short: 126 (0.0%) Reads written (passing filters): 129,489 (37.9%) Total basepairs processed: 95,139,757 bp Total written (filtered): 35,729,050 bp (37.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 129615 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 33 0.0 3 0 0 31 2 16 845 0.0 3 0 826 18 1 17 127336 0.0 3 124422 2812 90 12 18 334 0.0 3 37 288 7 2 19 100 0.0 3 3 4 82 11 20 759 0.0 3 671 13 1 74 21 2 0.0 3 1 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 27 4 0.0 3 1 2 0 1 29 1 0.0 3 0 0 0 1 34 7 0.0 3 0 0 0 7 39 1 0.0 3 1 53 1 0.0 3 0 0 0 1 55 10 0.0 3 0 0 0 10 58 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 91 13 0.0 3 0 0 0 13 94 4 0.0 3 0 0 0 4 101 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 1 130 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 146 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 8 0.0 3 0 0 0 8 151 2 0.0 3 0 0 0 2 159 12 0.0 3 0 0 0 12 160 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 164 9 0.0 3 0 0 0 9 165 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 187 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 1 207 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 40 0.0 3 0 0 0 40 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 4 0.0 3 0 0 0 4 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 250 1 0.0 3 1 270 2 0.0 3 0 0 0 2 278 1 0.0 3 0 0 0 1 279 7 0.0 3 0 0 0 7 280 8 0.0 3 0 0 0 8 281 6 0.0 3 0 0 0 6 282 4 0.0 3 0 0 0 4 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.47 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 129,489 Reads with adapters: 874 (0.7%) Reads written (passing filters): 129,489 (100.0%) Total basepairs processed: 35,729,050 bp Total written (filtered): 35,726,104 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 874 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.1% C: 7.0% G: 33.2% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 565 2023.3 0 565 4 297 505.8 0 297 5 10 126.5 0 10 6 1 31.6 0 1 7 1 7.9 0 1