This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 167.95 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 122,806 (3.4%) Reads that were too short: 15,288 (0.4%) Reads written (passing filters): 107,518 (3.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 29,581,193 bp (2.7%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 122806 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 608 887.3 0 608 7 1621 887.3 0 1621 8 49658 887.3 0 49658 9 37495 887.3 0 37495 10 252 887.3 0 252 11 67 887.3 0 67 12 17 887.3 0 17 13 35 887.3 0 35 14 31 887.3 0 31 15 52 887.3 0 52 16 48 887.3 0 48 17 41 887.3 0 41 18 56 887.3 0 56 19 88 887.3 0 88 20 58 887.3 0 58 21 23 887.3 0 23 22 22 887.3 0 22 23 31 887.3 0 31 24 28 887.3 0 28 25 22 887.3 0 22 26 30 887.3 0 30 27 35 887.3 0 35 28 150 887.3 0 150 29 215 887.3 0 215 30 89 887.3 0 89 31 19 887.3 0 19 32 37 887.3 0 37 33 35 887.3 0 35 34 38 887.3 0 38 35 47 887.3 0 47 36 37 887.3 0 37 37 45 887.3 0 45 38 29 887.3 0 29 39 20 887.3 0 20 40 17 887.3 0 17 41 27 887.3 0 27 42 35 887.3 0 35 43 66 887.3 0 66 44 121 887.3 0 121 45 755 887.3 0 755 46 457 887.3 0 457 47 641 887.3 0 641 48 23 887.3 0 23 49 20 887.3 0 20 50 17 887.3 0 17 51 27 887.3 0 27 52 24 887.3 0 24 53 24 887.3 0 24 54 20 887.3 0 20 55 20 887.3 0 20 56 19 887.3 0 19 57 24 887.3 0 24 58 21 887.3 0 21 59 17 887.3 0 17 60 24 887.3 0 24 61 20 887.3 0 20 62 19 887.3 0 19 63 29 887.3 0 29 64 23 887.3 0 23 65 29 887.3 0 29 66 28 887.3 0 28 67 23 887.3 0 23 68 88 887.3 0 88 69 61 887.3 0 61 70 46 887.3 0 46 71 151 887.3 0 151 72 182 887.3 0 182 73 140 887.3 0 140 74 74 887.3 0 74 75 43 887.3 0 43 76 156 887.3 0 156 77 30 887.3 0 30 78 34 887.3 0 34 79 34 887.3 0 34 80 59 887.3 0 59 81 75 887.3 0 75 82 377 887.3 0 377 83 134 887.3 0 134 84 103 887.3 0 103 85 146 887.3 0 146 86 93 887.3 0 93 87 27 887.3 0 27 88 53 887.3 0 53 89 35 887.3 0 35 90 24 887.3 0 24 91 42 887.3 0 42 92 39 887.3 0 39 93 23 887.3 0 23 94 36 887.3 0 36 95 272 887.3 0 272 96 368 887.3 0 368 97 210 887.3 0 210 98 41 887.3 0 41 99 30 887.3 0 30 100 32 887.3 0 32 101 37 887.3 0 37 102 28 887.3 0 28 103 29 887.3 0 29 104 24 887.3 0 24 105 29 887.3 0 29 106 23 887.3 0 23 107 25 887.3 0 25 108 19 887.3 0 19 109 29 887.3 0 29 110 42 887.3 0 42 111 45 887.3 0 45 112 81 887.3 0 81 113 484 887.3 0 484 114 428 887.3 0 428 115 318 887.3 0 318 116 261 887.3 0 261 117 49 887.3 0 49 118 32 887.3 0 32 119 36 887.3 0 36 120 56 887.3 0 56 121 56 887.3 0 56 122 47 887.3 0 47 123 55 887.3 0 55 124 71 887.3 0 71 125 47 887.3 0 47 126 71 887.3 0 71 127 43 887.3 0 43 128 55 887.3 0 55 129 65 887.3 0 65 130 76 887.3 0 76 131 63 887.3 0 63 132 83 887.3 0 83 133 51 887.3 0 51 134 102 887.3 0 102 135 196 887.3 0 196 136 235 887.3 0 235 137 258 887.3 0 258 138 751 887.3 0 751 139 421 887.3 0 421 140 198 887.3 0 198 141 68 887.3 0 68 142 102 887.3 0 102 143 171 887.3 0 171 144 127 887.3 0 127 145 84 887.3 0 84 146 114 887.3 0 114 147 81 887.3 0 81 148 59 887.3 0 59 149 76 887.3 0 76 150 64 887.3 0 64 151 63 887.3 0 63 152 68 887.3 0 68 153 35 887.3 0 35 154 50 887.3 0 50 155 35 887.3 0 35 156 48 887.3 0 48 157 48 887.3 0 48 158 35 887.3 0 35 159 48 887.3 0 48 160 27 887.3 0 27 161 38 887.3 0 38 162 114 887.3 0 114 163 97 887.3 0 97 164 122 887.3 0 122 165 50 887.3 0 50 166 53 887.3 0 53 167 171 887.3 0 171 168 257 887.3 0 257 169 191 887.3 0 191 170 103 887.3 0 103 171 42 887.3 0 42 172 44 887.3 0 44 173 46 887.3 0 46 174 46 887.3 0 46 175 28 887.3 0 28 176 29 887.3 0 29 177 52 887.3 0 52 178 70 887.3 0 70 179 121 887.3 0 121 180 127 887.3 0 127 181 85 887.3 0 85 182 51 887.3 0 51 183 81 887.3 0 81 184 412 887.3 0 412 185 485 887.3 0 485 186 367 887.3 0 367 187 69 887.3 0 69 188 43 887.3 0 43 189 47 887.3 0 47 190 59 887.3 0 59 191 53 887.3 0 53 192 39 887.3 0 39 193 44 887.3 0 44 194 45 887.3 0 45 195 49 887.3 0 49 196 32 887.3 0 32 197 37 887.3 0 37 198 64 887.3 0 64 199 132 887.3 0 132 200 122 887.3 0 122 201 72 887.3 0 72 202 63 887.3 0 63 203 68 887.3 0 68 204 258 887.3 0 258 205 293 887.3 0 293 206 239 887.3 0 239 207 105 887.3 0 105 208 95 887.3 0 95 209 85 887.3 0 85 210 100 887.3 0 100 211 74 887.3 0 74 212 61 887.3 0 61 213 68 887.3 0 68 214 88 887.3 0 88 215 133 887.3 0 133 216 142 887.3 0 142 217 68 887.3 0 68 218 55 887.3 0 55 219 55 887.3 0 55 220 66 887.3 0 66 221 67 887.3 0 67 222 77 887.3 0 77 223 131 887.3 0 131 224 330 887.3 0 330 225 2387 887.3 0 2387 226 3075 887.3 0 3075 227 1703 887.3 0 1703 228 594 887.3 0 594 229 71 887.3 0 71 230 44 887.3 0 44 231 53 887.3 0 53 232 64 887.3 0 64 233 46 887.3 0 46 234 66 887.3 0 66 235 100 887.3 0 100 236 120 887.3 0 120 237 51 887.3 0 51 238 40 887.3 0 40 239 92 887.3 0 92 240 86 887.3 0 86 241 79 887.3 0 79 242 72 887.3 0 72 243 106 887.3 0 106 244 92 887.3 0 92 245 44 887.3 0 44 246 57 887.3 0 57 247 43 887.3 0 43 248 55 887.3 0 55 249 48 887.3 0 48 250 37 887.3 0 37 251 26 887.3 0 26 252 38 887.3 0 38 253 52 887.3 0 52 254 44 887.3 0 44 255 56 887.3 0 56 256 42 887.3 0 42 257 61 887.3 0 61 258 56 887.3 0 56 259 115 887.3 0 115 260 68 887.3 0 68 261 53 887.3 0 53 262 45 887.3 0 45 263 45 887.3 0 45 264 70 887.3 0 70 265 97 887.3 0 97 266 89 887.3 0 89 267 39 887.3 0 39 268 41 887.3 0 41 269 63 887.3 0 63 270 42 887.3 0 42 271 131 887.3 0 131 272 81 887.3 0 81 273 66 887.3 0 66 274 52 887.3 0 52 275 59 887.3 0 59 276 48 887.3 0 48 277 36 887.3 0 36 278 44 887.3 0 44 279 94 887.3 0 94 280 98 887.3 0 98 281 148 887.3 0 148 282 34 887.3 0 34 283 36 887.3 0 36 284 32 887.3 0 32 285 41 887.3 0 41 286 52 887.3 0 52 287 35 887.3 0 35 288 30 887.3 0 30 289 58 887.3 0 58 290 51 887.3 0 51 291 51 887.3 0 51 292 47 887.3 0 47 293 59 887.3 0 59 294 58 887.3 0 58 295 67 887.3 0 67 296 75 887.3 0 75 297 82 887.3 0 82 298 152 887.3 0 152 299 87 887.3 0 87 300 60 887.3 0 60 301 236 887.3 0 236 Finished in 8.47 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 107,518 Reads with adapters: 42,162 (39.2%) Reads that were too short: 56 (0.1%) Reads written (passing filters): 42,106 (39.2%) Total basepairs processed: 29,581,193 bp Total written (filtered): 11,298,772 bp (38.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42162 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 6 1 18 19 0.0 3 0 0 13 4 2 19 391 0.0 3 0 358 15 14 4 20 39981 0.0 4 38369 1323 220 52 17 21 120 0.0 4 33 79 6 1 1 22 17 0.0 4 3 0 11 0 3 23 79 0.0 4 0 0 0 51 28 24 1 0.0 4 1 26 1 0.0 4 1 27 1 0.0 4 0 1 29 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 19 0.0 4 0 0 0 0 19 92 1 0.0 4 1 99 1 0.0 4 0 0 0 1 102 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 24 0.0 4 0 0 0 0 24 127 1427 0.0 4 0 0 0 0 1427 128 5 0.0 4 0 0 0 0 5 129 2 0.0 4 0 0 0 0 2 130 4 0.0 4 0 0 0 0 4 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 0 0 0 2 186 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 10 0.0 4 0 0 0 0 10 229 6 0.0 4 0 0 0 0 6 230 2 0.0 4 0 0 0 1 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.56 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 42,106 Reads with adapters: 8,478 (20.1%) Reads written (passing filters): 42,106 (100.0%) Total basepairs processed: 11,298,772 bp Total written (filtered): 11,213,181 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8478 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 5.7% G: 91.2% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 21 657.9 0 21 4 22 164.5 0 22 6 2 10.3 0 2 7 2 2.6 0 2 8 2 0.6 0 2 9 1965 0.2 0 80 1885 10 5040 0.0 1 252 4788 11 1383 0.0 1 19 1364 12 18 0.0 1 0 18 13 10 0.0 1 0 10 14 2 0.0 1 0 2 15 1 0.0 1 0 1 150 5 0.0 1 2 3 167 1 0.0 1 1 189 2 0.0 1 0 2 208 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.