This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 181.95 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 916,079 (25.2%) Reads that were too short: 19,280 (0.5%) Reads written (passing filters): 896,799 (24.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 172,249,922 bp (15.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 916079 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1170 887.3 0 1170 7 89368 887.3 0 89368 8 101436 887.3 0 101436 9 225 887.3 0 225 10 113 887.3 0 113 11 124 887.3 0 124 12 137 887.3 0 137 13 77 887.3 0 77 14 96 887.3 0 96 15 99 887.3 0 99 16 100 887.3 0 100 17 117 887.3 0 117 18 96 887.3 0 96 19 66 887.3 0 66 20 85 887.3 0 85 21 73 887.3 0 73 22 69 887.3 0 69 23 90 887.3 0 90 24 77 887.3 0 77 25 106 887.3 0 106 26 194 887.3 0 194 27 157 887.3 0 157 28 124 887.3 0 124 29 167 887.3 0 167 30 142 887.3 0 142 31 84 887.3 0 84 32 91 887.3 0 91 33 86 887.3 0 86 34 87 887.3 0 87 35 139 887.3 0 139 36 126 887.3 0 126 37 162 887.3 0 162 38 98 887.3 0 98 39 104 887.3 0 104 40 121 887.3 0 121 41 91 887.3 0 91 42 109 887.3 0 109 43 136 887.3 0 136 44 99 887.3 0 99 45 109 887.3 0 109 46 98 887.3 0 98 47 92 887.3 0 92 48 95 887.3 0 95 49 95 887.3 0 95 50 138 887.3 0 138 51 120 887.3 0 120 52 131 887.3 0 131 53 114 887.3 0 114 54 126 887.3 0 126 55 122 887.3 0 122 56 144 887.3 0 144 57 207 887.3 0 207 58 222 887.3 0 222 59 116 887.3 0 116 60 151 887.3 0 151 61 164 887.3 0 164 62 183 887.3 0 183 63 205 887.3 0 205 64 126 887.3 0 126 65 111 887.3 0 111 66 131 887.3 0 131 67 111 887.3 0 111 68 183 887.3 0 183 69 215 887.3 0 215 70 120 887.3 0 120 71 137 887.3 0 137 72 153 887.3 0 153 73 116 887.3 0 116 74 194 887.3 0 194 75 223 887.3 0 223 76 184 887.3 0 184 77 185 887.3 0 185 78 250 887.3 0 250 79 1172 887.3 0 1172 80 778 887.3 0 778 81 1806 887.3 0 1806 82 785 887.3 0 785 83 970 887.3 0 970 84 486 887.3 0 486 85 533 887.3 0 533 86 478 887.3 0 478 87 287 887.3 0 287 88 212 887.3 0 212 89 241 887.3 0 241 90 302 887.3 0 302 91 226 887.3 0 226 92 297 887.3 0 297 93 303 887.3 0 303 94 486 887.3 0 486 95 346 887.3 0 346 96 311 887.3 0 311 97 265 887.3 0 265 98 309 887.3 0 309 99 261 887.3 0 261 100 299 887.3 0 299 101 541 887.3 0 541 102 404 887.3 0 404 103 379 887.3 0 379 104 507 887.3 0 507 105 1092 887.3 0 1092 106 340 887.3 0 340 107 481 887.3 0 481 108 708 887.3 0 708 109 491 887.3 0 491 110 516 887.3 0 516 111 828 887.3 0 828 112 818 887.3 0 818 113 946 887.3 0 946 114 799 887.3 0 799 115 1568 887.3 0 1568 116 1755 887.3 0 1755 117 3942 887.3 0 3942 118 51650 887.3 0 51650 119 59899 887.3 0 59899 120 77788 887.3 0 77788 121 3042 887.3 0 3042 122 3387 887.3 0 3387 123 2966 887.3 0 2966 124 603 887.3 0 603 125 381 887.3 0 381 126 407 887.3 0 407 127 581 887.3 0 581 128 511 887.3 0 511 129 552 887.3 0 552 130 625 887.3 0 625 131 806 887.3 0 806 132 1844 887.3 0 1844 133 806 887.3 0 806 134 921 887.3 0 921 135 1445 887.3 0 1445 136 1013 887.3 0 1013 137 1339 887.3 0 1339 138 2055 887.3 0 2055 139 8468 887.3 0 8468 140 23067 887.3 0 23067 141 26890 887.3 0 26890 142 13191 887.3 0 13191 143 3323 887.3 0 3323 144 7587 887.3 0 7587 145 99803 887.3 0 99803 146 101058 887.3 0 101058 147 92111 887.3 0 92111 148 6256 887.3 0 6256 149 3481 887.3 0 3481 150 962 887.3 0 962 151 504 887.3 0 504 152 788 887.3 0 788 153 886 887.3 0 886 154 789 887.3 0 789 155 1323 887.3 0 1323 156 983 887.3 0 983 157 2575 887.3 0 2575 158 2399 887.3 0 2399 159 2428 887.3 0 2428 160 3573 887.3 0 3573 161 1711 887.3 0 1711 162 661 887.3 0 661 163 1033 887.3 0 1033 164 2904 887.3 0 2904 165 16780 887.3 0 16780 166 16687 887.3 0 16687 167 6430 887.3 0 6430 168 399 887.3 0 399 169 308 887.3 0 308 170 208 887.3 0 208 171 197 887.3 0 197 172 211 887.3 0 211 173 184 887.3 0 184 174 236 887.3 0 236 175 187 887.3 0 187 176 185 887.3 0 185 177 194 887.3 0 194 178 186 887.3 0 186 179 182 887.3 0 182 180 154 887.3 0 154 181 181 887.3 0 181 182 212 887.3 0 212 183 247 887.3 0 247 184 194 887.3 0 194 185 194 887.3 0 194 186 189 887.3 0 189 187 183 887.3 0 183 188 150 887.3 0 150 189 152 887.3 0 152 190 171 887.3 0 171 191 166 887.3 0 166 192 197 887.3 0 197 193 282 887.3 0 282 194 355 887.3 0 355 195 236 887.3 0 236 196 192 887.3 0 192 197 230 887.3 0 230 198 183 887.3 0 183 199 235 887.3 0 235 200 214 887.3 0 214 201 153 887.3 0 153 202 181 887.3 0 181 203 141 887.3 0 141 204 180 887.3 0 180 205 176 887.3 0 176 206 194 887.3 0 194 207 210 887.3 0 210 208 167 887.3 0 167 209 289 887.3 0 289 210 199 887.3 0 199 211 200 887.3 0 200 212 165 887.3 0 165 213 162 887.3 0 162 214 174 887.3 0 174 215 178 887.3 0 178 216 320 887.3 0 320 217 601 887.3 0 601 218 705 887.3 0 705 219 510 887.3 0 510 220 212 887.3 0 212 221 208 887.3 0 208 222 142 887.3 0 142 223 172 887.3 0 172 224 148 887.3 0 148 225 157 887.3 0 157 226 278 887.3 0 278 227 271 887.3 0 271 228 204 887.3 0 204 229 133 887.3 0 133 230 160 887.3 0 160 231 155 887.3 0 155 232 133 887.3 0 133 233 134 887.3 0 134 234 170 887.3 0 170 235 141 887.3 0 141 236 132 887.3 0 132 237 196 887.3 0 196 238 154 887.3 0 154 239 159 887.3 0 159 240 164 887.3 0 164 241 202 887.3 0 202 242 125 887.3 0 125 243 165 887.3 0 165 244 142 887.3 0 142 245 152 887.3 0 152 246 165 887.3 0 165 247 179 887.3 0 179 248 165 887.3 0 165 249 139 887.3 0 139 250 127 887.3 0 127 251 127 887.3 0 127 252 129 887.3 0 129 253 133 887.3 0 133 254 128 887.3 0 128 255 159 887.3 0 159 256 143 887.3 0 143 257 143 887.3 0 143 258 116 887.3 0 116 259 127 887.3 0 127 260 197 887.3 0 197 261 180 887.3 0 180 262 187 887.3 0 187 263 183 887.3 0 183 264 188 887.3 0 188 265 202 887.3 0 202 266 185 887.3 0 185 267 159 887.3 0 159 268 144 887.3 0 144 269 121 887.3 0 121 270 151 887.3 0 151 271 150 887.3 0 150 272 141 887.3 0 141 273 129 887.3 0 129 274 139 887.3 0 139 275 181 887.3 0 181 276 131 887.3 0 131 277 184 887.3 0 184 278 152 887.3 0 152 279 130 887.3 0 130 280 127 887.3 0 127 281 172 887.3 0 172 282 140 887.3 0 140 283 142 887.3 0 142 284 147 887.3 0 147 285 156 887.3 0 156 286 134 887.3 0 134 287 168 887.3 0 168 288 204 887.3 0 204 289 192 887.3 0 192 290 186 887.3 0 186 291 182 887.3 0 182 292 190 887.3 0 190 293 163 887.3 0 163 294 171 887.3 0 171 295 162 887.3 0 162 296 190 887.3 0 190 297 207 887.3 0 207 298 208 887.3 0 208 299 218 887.3 0 218 300 239 887.3 0 239 301 1207 887.3 0 1207 Finished in 50.85 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 896,799 Reads with adapters: 93,879 (10.5%) Reads that were too short: 494 (0.1%) Reads written (passing filters): 93,385 (10.4%) Total basepairs processed: 172,249,922 bp Total written (filtered): 25,073,352 bp (14.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 93879 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 10 16 401 0.0 3 0 393 8 17 85459 0.0 3 83825 1577 47 10 18 188 0.0 3 18 165 4 1 19 2386 0.0 3 2301 52 28 5 20 35 0.0 3 6 5 0 24 23 15 0.0 3 2 0 0 13 25 4 0.0 3 1 0 0 3 26 1477 0.0 3 0 0 4 1473 27 19 0.0 3 0 0 0 19 28 2 0.0 3 0 0 0 2 29 208 0.0 3 1 0 5 202 30 2 0.0 3 0 0 0 2 31 1 0.0 3 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 1 35 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 47 5 0.0 3 0 0 0 5 48 5 0.0 3 0 0 0 5 50 71 0.0 3 0 0 0 71 52 33 0.0 3 0 0 0 33 53 65 0.0 3 0 0 0 65 54 3 0.0 3 0 0 0 3 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 1 60 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 872 0.0 3 0 0 3 869 73 179 0.0 3 0 0 1 178 74 1799 0.0 3 0 0 2 1797 76 13 0.0 3 0 0 0 13 77 100 0.0 3 0 0 2 98 80 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 12 0.0 3 0 0 0 12 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 3 0.0 3 0 0 0 3 101 2 0.0 3 0 0 0 2 103 1 0.0 3 0 1 105 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 110 3 0.0 3 0 0 0 3 111 14 0.0 3 0 0 0 14 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 114 6 0.0 3 0 0 0 6 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 7 0.0 3 0 0 0 7 125 6 0.0 3 0 0 0 6 126 3 0.0 3 0 0 0 3 127 5 0.0 3 0 0 0 5 128 23 0.0 3 0 0 0 23 129 7 0.0 3 0 0 0 7 131 1 0.0 3 0 0 0 1 133 37 0.0 3 0 0 0 37 134 3 0.0 3 0 0 0 3 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 58 0.0 3 0 0 1 57 139 15 0.0 3 0 0 0 15 140 11 0.0 3 0 0 2 9 141 47 0.0 3 0 0 1 46 142 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 146 10 0.0 3 0 0 0 10 147 20 0.0 3 0 0 1 19 148 4 0.0 3 0 0 0 4 150 2 0.0 3 0 0 0 2 151 8 0.0 3 0 0 0 8 152 2 0.0 3 1 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 51 0.0 3 0 0 0 51 162 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 165 2 0.0 3 0 0 0 2 166 11 0.0 3 0 0 0 11 167 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 5 0.0 3 0 0 0 5 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 1 196 5 0.0 3 0 0 0 5 197 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 1 216 1 0.0 3 0 0 0 1 217 32 0.0 3 0 0 0 32 221 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 236 2 0.0 3 0 0 0 2 237 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 0 1 281 7 0.0 3 0 0 0 7 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.26 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 93,385 Reads with adapters: 350 (0.4%) Reads written (passing filters): 93,385 (100.0%) Total basepairs processed: 25,073,352 bp Total written (filtered): 25,072,265 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 350 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.3% C: 27.1% G: 45.7% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 321 1459.1 0 321 4 24 364.8 0 24 5 2 91.2 0 2 6 3 22.8 0 3