This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.53 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 347,228 (9.6%) Reads that were too short: 17,062 (0.5%) Reads written (passing filters): 330,166 (9.1%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 62,309,250 bp (5.7%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 347228 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1780 887.3 0 1780 7 3202 887.3 0 3202 8 3421 887.3 0 3421 9 38 887.3 0 38 10 30 887.3 0 30 11 87 887.3 0 87 12 88 887.3 0 88 13 99 887.3 0 99 14 58 887.3 0 58 15 37 887.3 0 37 16 22 887.3 0 22 17 26 887.3 0 26 18 43 887.3 0 43 19 38 887.3 0 38 20 76 887.3 0 76 21 64 887.3 0 64 22 37 887.3 0 37 23 42 887.3 0 42 24 26 887.3 0 26 25 58 887.3 0 58 26 81 887.3 0 81 27 76 887.3 0 76 28 43 887.3 0 43 29 54 887.3 0 54 30 55 887.3 0 55 31 43 887.3 0 43 32 44 887.3 0 44 33 42 887.3 0 42 34 32 887.3 0 32 35 61 887.3 0 61 36 69 887.3 0 69 37 86 887.3 0 86 38 70 887.3 0 70 39 55 887.3 0 55 40 56 887.3 0 56 41 55 887.3 0 55 42 47 887.3 0 47 43 108 887.3 0 108 44 53 887.3 0 53 45 40 887.3 0 40 46 77 887.3 0 77 47 76 887.3 0 76 48 61 887.3 0 61 49 46 887.3 0 46 50 59 887.3 0 59 51 51 887.3 0 51 52 59 887.3 0 59 53 66 887.3 0 66 54 96 887.3 0 96 55 84 887.3 0 84 56 81 887.3 0 81 57 72 887.3 0 72 58 54 887.3 0 54 59 44 887.3 0 44 60 118 887.3 0 118 61 88 887.3 0 88 62 110 887.3 0 110 63 65 887.3 0 65 64 86 887.3 0 86 65 308 887.3 0 308 66 208 887.3 0 208 67 162 887.3 0 162 68 50 887.3 0 50 69 56 887.3 0 56 70 62 887.3 0 62 71 59 887.3 0 59 72 75 887.3 0 75 73 52 887.3 0 52 74 137 887.3 0 137 75 37 887.3 0 37 76 49 887.3 0 49 77 78 887.3 0 78 78 167 887.3 0 167 79 122 887.3 0 122 80 397 887.3 0 397 81 141 887.3 0 141 82 117 887.3 0 117 83 77 887.3 0 77 84 103 887.3 0 103 85 86 887.3 0 86 86 151 887.3 0 151 87 159 887.3 0 159 88 204 887.3 0 204 89 222 887.3 0 222 90 451 887.3 0 451 91 1252 887.3 0 1252 92 252 887.3 0 252 93 405 887.3 0 405 94 713 887.3 0 713 95 348 887.3 0 348 96 388 887.3 0 388 97 731 887.3 0 731 98 794 887.3 0 794 99 851 887.3 0 851 100 804 887.3 0 804 101 1414 887.3 0 1414 102 1836 887.3 0 1836 103 3889 887.3 0 3889 104 90067 887.3 0 90067 105 88822 887.3 0 88822 106 55138 887.3 0 55138 107 228 887.3 0 228 108 59 887.3 0 59 109 49 887.3 0 49 110 51 887.3 0 51 111 72 887.3 0 72 112 54 887.3 0 54 113 54 887.3 0 54 114 79 887.3 0 79 115 59 887.3 0 59 116 76 887.3 0 76 117 61 887.3 0 61 118 46 887.3 0 46 119 85 887.3 0 85 120 82 887.3 0 82 121 89 887.3 0 89 122 61 887.3 0 61 123 98 887.3 0 98 124 403 887.3 0 403 125 311 887.3 0 311 126 570 887.3 0 570 127 78 887.3 0 78 128 50 887.3 0 50 129 110 887.3 0 110 130 88 887.3 0 88 131 59 887.3 0 59 132 134 887.3 0 134 133 61 887.3 0 61 134 56 887.3 0 56 135 46 887.3 0 46 136 64 887.3 0 64 137 63 887.3 0 63 138 57 887.3 0 57 139 113 887.3 0 113 140 94 887.3 0 94 141 208 887.3 0 208 142 168 887.3 0 168 143 74 887.3 0 74 144 81 887.3 0 81 145 76 887.3 0 76 146 122 887.3 0 122 147 180 887.3 0 180 148 229 887.3 0 229 149 72 887.3 0 72 150 81 887.3 0 81 151 201 887.3 0 201 152 293 887.3 0 293 153 302 887.3 0 302 154 236 887.3 0 236 155 272 887.3 0 272 156 395 887.3 0 395 157 569 887.3 0 569 158 999 887.3 0 999 159 17512 887.3 0 17512 160 16240 887.3 0 16240 161 19075 887.3 0 19075 162 129 887.3 0 129 163 70 887.3 0 70 164 55 887.3 0 55 165 67 887.3 0 67 166 74 887.3 0 74 167 193 887.3 0 193 168 322 887.3 0 322 169 258 887.3 0 258 170 70 887.3 0 70 171 103 887.3 0 103 172 171 887.3 0 171 173 135 887.3 0 135 174 87 887.3 0 87 175 56 887.3 0 56 176 73 887.3 0 73 177 75 887.3 0 75 178 53 887.3 0 53 179 63 887.3 0 63 180 91 887.3 0 91 181 117 887.3 0 117 182 65 887.3 0 65 183 82 887.3 0 82 184 123 887.3 0 123 185 180 887.3 0 180 186 115 887.3 0 115 187 210 887.3 0 210 188 200 887.3 0 200 189 169 887.3 0 169 190 142 887.3 0 142 191 51 887.3 0 51 192 70 887.3 0 70 193 62 887.3 0 62 194 42 887.3 0 42 195 68 887.3 0 68 196 61 887.3 0 61 197 101 887.3 0 101 198 111 887.3 0 111 199 176 887.3 0 176 200 803 887.3 0 803 201 759 887.3 0 759 202 936 887.3 0 936 203 56 887.3 0 56 204 73 887.3 0 73 205 82 887.3 0 82 206 89 887.3 0 89 207 109 887.3 0 109 208 89 887.3 0 89 209 94 887.3 0 94 210 53 887.3 0 53 211 63 887.3 0 63 212 42 887.3 0 42 213 70 887.3 0 70 214 72 887.3 0 72 215 52 887.3 0 52 216 97 887.3 0 97 217 76 887.3 0 76 218 103 887.3 0 103 219 130 887.3 0 130 220 164 887.3 0 164 221 96 887.3 0 96 222 56 887.3 0 56 223 77 887.3 0 77 224 71 887.3 0 71 225 63 887.3 0 63 226 114 887.3 0 114 227 106 887.3 0 106 228 131 887.3 0 131 229 127 887.3 0 127 230 98 887.3 0 98 231 70 887.3 0 70 232 72 887.3 0 72 233 64 887.3 0 64 234 75 887.3 0 75 235 64 887.3 0 64 236 70 887.3 0 70 237 93 887.3 0 93 238 152 887.3 0 152 239 138 887.3 0 138 240 817 887.3 0 817 241 1159 887.3 0 1159 242 1159 887.3 0 1159 243 308 887.3 0 308 244 79 887.3 0 79 245 73 887.3 0 73 246 72 887.3 0 72 247 94 887.3 0 94 248 95 887.3 0 95 249 91 887.3 0 91 250 61 887.3 0 61 251 76 887.3 0 76 252 78 887.3 0 78 253 74 887.3 0 74 254 84 887.3 0 84 255 118 887.3 0 118 256 112 887.3 0 112 257 121 887.3 0 121 258 93 887.3 0 93 259 74 887.3 0 74 260 91 887.3 0 91 261 95 887.3 0 95 262 67 887.3 0 67 263 85 887.3 0 85 264 61 887.3 0 61 265 82 887.3 0 82 266 97 887.3 0 97 267 110 887.3 0 110 268 109 887.3 0 109 269 119 887.3 0 119 270 122 887.3 0 122 271 106 887.3 0 106 272 104 887.3 0 104 273 122 887.3 0 122 274 216 887.3 0 216 275 199 887.3 0 199 276 219 887.3 0 219 277 215 887.3 0 215 278 938 887.3 0 938 279 415 887.3 0 415 280 860 887.3 0 860 281 363 887.3 0 363 282 760 887.3 0 760 283 395 887.3 0 395 284 83 887.3 0 83 285 88 887.3 0 88 286 67 887.3 0 67 287 89 887.3 0 89 288 99 887.3 0 99 289 88 887.3 0 88 290 115 887.3 0 115 291 99 887.3 0 99 292 104 887.3 0 104 293 111 887.3 0 111 294 145 887.3 0 145 295 183 887.3 0 183 296 120 887.3 0 120 297 76 887.3 0 76 298 72 887.3 0 72 299 117 887.3 0 117 300 184 887.3 0 184 301 247 887.3 0 247 Finished in 17.99 s (54 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 330,166 Reads with adapters: 5,133 (1.6%) Reads that were too short: 91 (0.0%) Reads written (passing filters): 5,042 (1.5%) Total basepairs processed: 62,309,250 bp Total written (filtered): 1,359,803 bp (2.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5133 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 30 0.0 3 0 29 1 17 2928 0.0 3 2851 75 1 1 18 19 0.0 3 13 6 19 175 0.0 3 156 19 20 1688 0.0 3 1643 37 0 8 21 1 0.0 3 0 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 1 29 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 44 6 0.0 3 0 0 0 6 46 32 0.0 3 0 0 0 32 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 2 0.0 3 1 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 75 0.0 3 0 0 3 72 88 29 0.0 3 0 0 1 28 89 1 0.0 3 0 0 0 1 90 21 0.0 3 0 0 2 19 91 11 0.0 3 0 0 1 10 100 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 4 0.0 3 0 0 0 4 114 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 1 1 133 2 0.0 3 0 0 1 1 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 4 0.0 3 0 0 0 4 139 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 147 2 0.0 3 0 0 0 2 148 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 188 10 0.0 3 0 0 1 9 189 37 0.0 3 0 0 3 34 206 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 5,042 Reads with adapters: 47 (0.9%) Reads written (passing filters): 5,042 (100.0%) Total basepairs processed: 1,359,803 bp Total written (filtered): 1,359,661 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 47 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.3% C: 10.6% G: 31.9% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 46 78.8 0 46 4 1 19.7 0 1