This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 172.18 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 236,195 (6.5%) Reads that were too short: 10,753 (0.3%) Reads written (passing filters): 225,442 (6.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 62,987,088 bp (5.8%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 236195 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1207 887.3 0 1207 7 4777 887.3 0 4777 8 103936 887.3 0 103936 9 89047 887.3 0 89047 10 174 887.3 0 174 11 42 887.3 0 42 12 32 887.3 0 32 13 37 887.3 0 37 14 19 887.3 0 19 15 36 887.3 0 36 16 13 887.3 0 13 17 18 887.3 0 18 18 13 887.3 0 13 19 24 887.3 0 24 20 14 887.3 0 14 21 18 887.3 0 18 22 20 887.3 0 20 23 8 887.3 0 8 24 7 887.3 0 7 25 13 887.3 0 13 26 17 887.3 0 17 27 18 887.3 0 18 28 18 887.3 0 18 29 9 887.3 0 9 30 23 887.3 0 23 31 14 887.3 0 14 32 14 887.3 0 14 33 14 887.3 0 14 34 31 887.3 0 31 35 20 887.3 0 20 36 16 887.3 0 16 37 17 887.3 0 17 38 19 887.3 0 19 39 16 887.3 0 16 40 43 887.3 0 43 41 22 887.3 0 22 42 23 887.3 0 23 43 16 887.3 0 16 44 21 887.3 0 21 45 28 887.3 0 28 46 32 887.3 0 32 47 66 887.3 0 66 48 28 887.3 0 28 49 117 887.3 0 117 50 39 887.3 0 39 51 14 887.3 0 14 52 17 887.3 0 17 53 27 887.3 0 27 54 27 887.3 0 27 55 23 887.3 0 23 56 17 887.3 0 17 57 18 887.3 0 18 58 31 887.3 0 31 59 36 887.3 0 36 60 59 887.3 0 59 61 37 887.3 0 37 62 39 887.3 0 39 63 20 887.3 0 20 64 19 887.3 0 19 65 16 887.3 0 16 66 389 887.3 0 389 67 248 887.3 0 248 68 255 887.3 0 255 69 8 887.3 0 8 70 30 887.3 0 30 71 16 887.3 0 16 72 14 887.3 0 14 73 21 887.3 0 21 74 19 887.3 0 19 75 14 887.3 0 14 76 17 887.3 0 17 77 28 887.3 0 28 78 14 887.3 0 14 79 18 887.3 0 18 80 22 887.3 0 22 81 21 887.3 0 21 82 44 887.3 0 44 83 40 887.3 0 40 84 93 887.3 0 93 85 122 887.3 0 122 86 18 887.3 0 18 87 15 887.3 0 15 88 59 887.3 0 59 89 13 887.3 0 13 90 36 887.3 0 36 91 374 887.3 0 374 92 86 887.3 0 86 93 112 887.3 0 112 94 67 887.3 0 67 95 37 887.3 0 37 96 24 887.3 0 24 97 15 887.3 0 15 98 28 887.3 0 28 99 23 887.3 0 23 100 14 887.3 0 14 101 22 887.3 0 22 102 23 887.3 0 23 103 37 887.3 0 37 104 56 887.3 0 56 105 41 887.3 0 41 106 51 887.3 0 51 107 67 887.3 0 67 108 52 887.3 0 52 109 58 887.3 0 58 110 96 887.3 0 96 111 787 887.3 0 787 112 441 887.3 0 441 113 766 887.3 0 766 114 167 887.3 0 167 115 456 887.3 0 456 116 820 887.3 0 820 117 6645 887.3 0 6645 118 1322 887.3 0 1322 119 2127 887.3 0 2127 120 41 887.3 0 41 121 38 887.3 0 38 122 22 887.3 0 22 123 26 887.3 0 26 124 44 887.3 0 44 125 62 887.3 0 62 126 638 887.3 0 638 127 166 887.3 0 166 128 175 887.3 0 175 129 43 887.3 0 43 130 58 887.3 0 58 131 71 887.3 0 71 132 105 887.3 0 105 133 64 887.3 0 64 134 33 887.3 0 33 135 37 887.3 0 37 136 46 887.3 0 46 137 35 887.3 0 35 138 99 887.3 0 99 139 41 887.3 0 41 140 35 887.3 0 35 141 41 887.3 0 41 142 46 887.3 0 46 143 30 887.3 0 30 144 56 887.3 0 56 145 59 887.3 0 59 146 111 887.3 0 111 147 95 887.3 0 95 148 119 887.3 0 119 149 79 887.3 0 79 150 127 887.3 0 127 151 1098 887.3 0 1098 152 1002 887.3 0 1002 153 680 887.3 0 680 154 237 887.3 0 237 155 29 887.3 0 29 156 42 887.3 0 42 157 42 887.3 0 42 158 28 887.3 0 28 159 47 887.3 0 47 160 47 887.3 0 47 161 37 887.3 0 37 162 184 887.3 0 184 163 106 887.3 0 106 164 117 887.3 0 117 165 35 887.3 0 35 166 44 887.3 0 44 167 27 887.3 0 27 168 32 887.3 0 32 169 51 887.3 0 51 170 33 887.3 0 33 171 28 887.3 0 28 172 33 887.3 0 33 173 56 887.3 0 56 174 32 887.3 0 32 175 22 887.3 0 22 176 35 887.3 0 35 177 33 887.3 0 33 178 34 887.3 0 34 179 165 887.3 0 165 180 149 887.3 0 149 181 92 887.3 0 92 182 75 887.3 0 75 183 171 887.3 0 171 184 248 887.3 0 248 185 70 887.3 0 70 186 24 887.3 0 24 187 45 887.3 0 45 188 40 887.3 0 40 189 60 887.3 0 60 190 46 887.3 0 46 191 85 887.3 0 85 192 69 887.3 0 69 193 49 887.3 0 49 194 25 887.3 0 25 195 30 887.3 0 30 196 29 887.3 0 29 197 42 887.3 0 42 198 48 887.3 0 48 199 51 887.3 0 51 200 41 887.3 0 41 201 31 887.3 0 31 202 27 887.3 0 27 203 45 887.3 0 45 204 45 887.3 0 45 205 50 887.3 0 50 206 47 887.3 0 47 207 29 887.3 0 29 208 48 887.3 0 48 209 23 887.3 0 23 210 65 887.3 0 65 211 91 887.3 0 91 212 144 887.3 0 144 213 78 887.3 0 78 214 66 887.3 0 66 215 59 887.3 0 59 216 69 887.3 0 69 217 157 887.3 0 157 218 303 887.3 0 303 219 361 887.3 0 361 220 308 887.3 0 308 221 67 887.3 0 67 222 27 887.3 0 27 223 57 887.3 0 57 224 120 887.3 0 120 225 87 887.3 0 87 226 98 887.3 0 98 227 77 887.3 0 77 228 106 887.3 0 106 229 103 887.3 0 103 230 59 887.3 0 59 231 304 887.3 0 304 232 299 887.3 0 299 233 373 887.3 0 373 234 218 887.3 0 218 235 154 887.3 0 154 236 112 887.3 0 112 237 110 887.3 0 110 238 70 887.3 0 70 239 81 887.3 0 81 240 63 887.3 0 63 241 45 887.3 0 45 242 86 887.3 0 86 243 91 887.3 0 91 244 117 887.3 0 117 245 192 887.3 0 192 246 210 887.3 0 210 247 246 887.3 0 246 248 211 887.3 0 211 249 155 887.3 0 155 250 85 887.3 0 85 251 93 887.3 0 93 252 114 887.3 0 114 253 93 887.3 0 93 254 139 887.3 0 139 255 110 887.3 0 110 256 61 887.3 0 61 257 72 887.3 0 72 258 51 887.3 0 51 259 59 887.3 0 59 260 40 887.3 0 40 261 47 887.3 0 47 262 35 887.3 0 35 263 39 887.3 0 39 264 66 887.3 0 66 265 84 887.3 0 84 266 74 887.3 0 74 267 76 887.3 0 76 268 67 887.3 0 67 269 40 887.3 0 40 270 58 887.3 0 58 271 98 887.3 0 98 272 109 887.3 0 109 273 101 887.3 0 101 274 60 887.3 0 60 275 40 887.3 0 40 276 51 887.3 0 51 277 44 887.3 0 44 278 96 887.3 0 96 279 133 887.3 0 133 280 161 887.3 0 161 281 81 887.3 0 81 282 150 887.3 0 150 283 209 887.3 0 209 284 171 887.3 0 171 285 98 887.3 0 98 286 104 887.3 0 104 287 125 887.3 0 125 288 88 887.3 0 88 289 73 887.3 0 73 290 65 887.3 0 65 291 122 887.3 0 122 292 113 887.3 0 113 293 200 887.3 0 200 294 118 887.3 0 118 295 79 887.3 0 79 296 80 887.3 0 80 297 52 887.3 0 52 298 89 887.3 0 89 299 128 887.3 0 128 300 130 887.3 0 130 301 129 887.3 0 129 Finished in 17.61 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 225,442 Reads with adapters: 95,427 (42.3%) Reads that were too short: 63 (0.0%) Reads written (passing filters): 95,364 (42.3%) Total basepairs processed: 62,987,088 bp Total written (filtered): 25,750,067 bp (40.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 95427 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 12 0.0 3 0 0 0 6 6 18 71 0.0 3 0 0 58 11 2 19 1056 0.0 3 0 939 61 34 22 20 91942 0.0 4 87836 3361 548 156 41 21 232 0.0 4 57 156 14 1 4 22 32 0.0 4 9 1 12 2 8 23 171 0.0 4 5 2 0 134 30 24 7 0.0 4 1 0 0 1 5 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 1 27 2 0.0 4 0 0 0 2 29 1 0.0 4 0 0 0 0 1 31 1 0.0 4 1 54 1 0.0 4 0 0 0 1 65 3 0.0 4 0 0 0 1 2 73 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 1 1 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 19 0.0 4 0 0 0 0 19 127 1800 0.0 4 0 0 0 0 1800 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 182 1 0.0 4 0 0 0 0 1 183 2 0.0 4 0 0 0 0 2 188 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 283 2 0.0 4 0 0 0 0 2 285 2 0.0 4 0 0 0 0 2 292 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.87 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 95,364 Reads with adapters: 20,814 (21.8%) Reads written (passing filters): 95,364 (100.0%) Total basepairs processed: 25,750,067 bp Total written (filtered): 25,541,680 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20814 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 5.7% G: 91.1% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 25 1490.1 0 25 4 25 372.5 0 25 5 2 93.1 0 2 6 4 23.3 0 4 7 3 5.8 0 3 8 5 1.5 0 5 9 5806 0.4 0 338 5468 10 12458 0.1 1 696 11762 11 2391 0.0 1 43 2348 12 37 0.0 1 1 36 13 35 0.0 1 1 34 14 1 0.0 1 0 1 28 1 0.0 1 0 1 142 1 0.0 1 1 147 1 0.0 1 1 150 4 0.0 1 3 1 169 2 0.0 1 2 170 1 0.0 1 1 208 8 0.0 1 7 1 209 1 0.0 1 1 257 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.