This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.15 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 231,688 (6.4%) Reads that were too short: 5,179 (0.1%) Reads written (passing filters): 226,509 (6.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 64,010,864 bp (5.9%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 231688 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 941 887.3 0 941 7 3259 887.3 0 3259 8 97112 887.3 0 97112 9 104756 887.3 0 104756 10 110 887.3 0 110 11 16 887.3 0 16 12 24 887.3 0 24 13 21 887.3 0 21 14 14 887.3 0 14 15 20 887.3 0 20 16 20 887.3 0 20 17 19 887.3 0 19 18 17 887.3 0 17 19 29 887.3 0 29 20 17 887.3 0 17 21 15 887.3 0 15 22 21 887.3 0 21 23 15 887.3 0 15 24 7 887.3 0 7 25 15 887.3 0 15 26 18 887.3 0 18 27 11 887.3 0 11 28 16 887.3 0 16 29 19 887.3 0 19 30 22 887.3 0 22 31 13 887.3 0 13 32 13 887.3 0 13 33 17 887.3 0 17 34 28 887.3 0 28 35 25 887.3 0 25 36 25 887.3 0 25 37 27 887.3 0 27 38 13 887.3 0 13 39 16 887.3 0 16 40 19 887.3 0 19 41 13 887.3 0 13 42 15 887.3 0 15 43 17 887.3 0 17 44 25 887.3 0 25 45 27 887.3 0 27 46 26 887.3 0 26 47 29 887.3 0 29 48 24 887.3 0 24 49 24 887.3 0 24 50 18 887.3 0 18 51 23 887.3 0 23 52 33 887.3 0 33 53 24 887.3 0 24 54 26 887.3 0 26 55 35 887.3 0 35 56 28 887.3 0 28 57 23 887.3 0 23 58 24 887.3 0 24 59 29 887.3 0 29 60 23 887.3 0 23 61 26 887.3 0 26 62 20 887.3 0 20 63 13 887.3 0 13 64 22 887.3 0 22 65 16 887.3 0 16 66 174 887.3 0 174 67 123 887.3 0 123 68 118 887.3 0 118 69 18 887.3 0 18 70 23 887.3 0 23 71 22 887.3 0 22 72 18 887.3 0 18 73 28 887.3 0 28 74 15 887.3 0 15 75 26 887.3 0 26 76 21 887.3 0 21 77 11 887.3 0 11 78 21 887.3 0 21 79 27 887.3 0 27 80 23 887.3 0 23 81 24 887.3 0 24 82 47 887.3 0 47 83 36 887.3 0 36 84 26 887.3 0 26 85 33 887.3 0 33 86 38 887.3 0 38 87 32 887.3 0 32 88 25 887.3 0 25 89 26 887.3 0 26 90 24 887.3 0 24 91 141 887.3 0 141 92 91 887.3 0 91 93 127 887.3 0 127 94 37 887.3 0 37 95 33 887.3 0 33 96 25 887.3 0 25 97 30 887.3 0 30 98 42 887.3 0 42 99 29 887.3 0 29 100 32 887.3 0 32 101 27 887.3 0 27 102 34 887.3 0 34 103 18 887.3 0 18 104 58 887.3 0 58 105 43 887.3 0 43 106 39 887.3 0 39 107 83 887.3 0 83 108 52 887.3 0 52 109 50 887.3 0 50 110 111 887.3 0 111 111 749 887.3 0 749 112 442 887.3 0 442 113 754 887.3 0 754 114 132 887.3 0 132 115 244 887.3 0 244 116 366 887.3 0 366 117 5826 887.3 0 5826 118 1281 887.3 0 1281 119 2333 887.3 0 2333 120 31 887.3 0 31 121 29 887.3 0 29 122 24 887.3 0 24 123 33 887.3 0 33 124 29 887.3 0 29 125 36 887.3 0 36 126 536 887.3 0 536 127 116 887.3 0 116 128 137 887.3 0 137 129 17 887.3 0 17 130 54 887.3 0 54 131 39 887.3 0 39 132 46 887.3 0 46 133 34 887.3 0 34 134 21 887.3 0 21 135 22 887.3 0 22 136 27 887.3 0 27 137 24 887.3 0 24 138 72 887.3 0 72 139 25 887.3 0 25 140 25 887.3 0 25 141 25 887.3 0 25 142 29 887.3 0 29 143 31 887.3 0 31 144 32 887.3 0 32 145 20 887.3 0 20 146 52 887.3 0 52 147 49 887.3 0 49 148 71 887.3 0 71 149 30 887.3 0 30 150 24 887.3 0 24 151 435 887.3 0 435 152 469 887.3 0 469 153 274 887.3 0 274 154 114 887.3 0 114 155 23 887.3 0 23 156 32 887.3 0 32 157 27 887.3 0 27 158 15 887.3 0 15 159 22 887.3 0 22 160 28 887.3 0 28 161 25 887.3 0 25 162 45 887.3 0 45 163 39 887.3 0 39 164 35 887.3 0 35 165 30 887.3 0 30 166 25 887.3 0 25 167 13 887.3 0 13 168 14 887.3 0 14 169 38 887.3 0 38 170 29 887.3 0 29 171 20 887.3 0 20 172 17 887.3 0 17 173 27 887.3 0 27 174 17 887.3 0 17 175 14 887.3 0 14 176 34 887.3 0 34 177 16 887.3 0 16 178 20 887.3 0 20 179 95 887.3 0 95 180 128 887.3 0 128 181 65 887.3 0 65 182 72 887.3 0 72 183 255 887.3 0 255 184 279 887.3 0 279 185 65 887.3 0 65 186 21 887.3 0 21 187 19 887.3 0 19 188 19 887.3 0 19 189 26 887.3 0 26 190 42 887.3 0 42 191 47 887.3 0 47 192 40 887.3 0 40 193 14 887.3 0 14 194 18 887.3 0 18 195 20 887.3 0 20 196 23 887.3 0 23 197 19 887.3 0 19 198 25 887.3 0 25 199 23 887.3 0 23 200 21 887.3 0 21 201 15 887.3 0 15 202 31 887.3 0 31 203 21 887.3 0 21 204 25 887.3 0 25 205 29 887.3 0 29 206 25 887.3 0 25 207 28 887.3 0 28 208 21 887.3 0 21 209 21 887.3 0 21 210 26 887.3 0 26 211 71 887.3 0 71 212 150 887.3 0 150 213 48 887.3 0 48 214 33 887.3 0 33 215 40 887.3 0 40 216 30 887.3 0 30 217 63 887.3 0 63 218 234 887.3 0 234 219 376 887.3 0 376 220 302 887.3 0 302 221 22 887.3 0 22 222 13 887.3 0 13 223 37 887.3 0 37 224 85 887.3 0 85 225 51 887.3 0 51 226 33 887.3 0 33 227 42 887.3 0 42 228 41 887.3 0 41 229 43 887.3 0 43 230 34 887.3 0 34 231 81 887.3 0 81 232 82 887.3 0 82 233 110 887.3 0 110 234 67 887.3 0 67 235 54 887.3 0 54 236 52 887.3 0 52 237 57 887.3 0 57 238 35 887.3 0 35 239 33 887.3 0 33 240 18 887.3 0 18 241 23 887.3 0 23 242 23 887.3 0 23 243 23 887.3 0 23 244 45 887.3 0 45 245 60 887.3 0 60 246 60 887.3 0 60 247 86 887.3 0 86 248 115 887.3 0 115 249 70 887.3 0 70 250 33 887.3 0 33 251 43 887.3 0 43 252 40 887.3 0 40 253 42 887.3 0 42 254 56 887.3 0 56 255 42 887.3 0 42 256 27 887.3 0 27 257 31 887.3 0 31 258 19 887.3 0 19 259 26 887.3 0 26 260 32 887.3 0 32 261 18 887.3 0 18 262 18 887.3 0 18 263 19 887.3 0 19 264 27 887.3 0 27 265 22 887.3 0 22 266 33 887.3 0 33 267 46 887.3 0 46 268 29 887.3 0 29 269 28 887.3 0 28 270 28 887.3 0 28 271 41 887.3 0 41 272 43 887.3 0 43 273 43 887.3 0 43 274 24 887.3 0 24 275 30 887.3 0 30 276 30 887.3 0 30 277 46 887.3 0 46 278 65 887.3 0 65 279 80 887.3 0 80 280 85 887.3 0 85 281 34 887.3 0 34 282 54 887.3 0 54 283 59 887.3 0 59 284 60 887.3 0 60 285 47 887.3 0 47 286 55 887.3 0 55 287 57 887.3 0 57 288 40 887.3 0 40 289 40 887.3 0 40 290 22 887.3 0 22 291 58 887.3 0 58 292 40 887.3 0 40 293 69 887.3 0 69 294 53 887.3 0 53 295 24 887.3 0 24 296 29 887.3 0 29 297 34 887.3 0 34 298 32 887.3 0 32 299 61 887.3 0 61 300 40 887.3 0 40 301 56 887.3 0 56 Finished in 16.63 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 226,509 Reads with adapters: 96,625 (42.7%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 96,577 (42.6%) Total basepairs processed: 64,010,864 bp Total written (filtered): 26,644,790 bp (41.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 96625 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 25 0.0 3 0 0 24 1 16 603 0.0 3 0 588 15 17 95568 0.0 3 93663 1848 50 7 18 207 0.0 3 23 176 6 2 19 38 0.0 3 3 0 31 4 20 31 0.0 3 1 1 0 29 21 1 0.0 3 0 0 0 1 24 2 0.0 3 2 32 2 0.0 3 1 0 0 1 35 1 0.0 3 1 38 1 0.0 3 1 40 9 0.0 3 0 0 0 9 43 3 0.0 3 0 0 0 3 54 46 0.0 3 0 0 0 46 56 2 0.0 3 0 0 0 2 57 10 0.0 3 0 0 0 10 58 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 86 1 0.0 3 0 1 91 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 105 5 0.0 3 0 0 0 5 106 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 0 2 142 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 166 11 0.0 3 0 0 0 11 167 1 0.0 3 0 0 0 1 169 10 0.0 3 0 0 0 10 170 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.60 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 96,577 Reads with adapters: 458 (0.5%) Reads written (passing filters): 96,577 (100.0%) Total basepairs processed: 26,644,790 bp Total written (filtered): 26,643,197 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 458 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.3% C: 4.8% G: 40.6% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 245 1509.0 0 245 4 207 377.3 0 207 5 6 94.3 0 6