This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 179.36 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 695,183 (19.1%) Reads that were too short: 51,664 (1.4%) Reads written (passing filters): 643,519 (17.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 161,295,636 bp (14.7%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 695183 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1789 887.3 0 1789 7 65121 887.3 0 65121 8 51743 887.3 0 51743 9 293 887.3 0 293 10 256 887.3 0 256 11 201 887.3 0 201 12 238 887.3 0 238 13 398 887.3 0 398 14 485 887.3 0 485 15 519 887.3 0 519 16 453 887.3 0 453 17 902 887.3 0 902 18 1086 887.3 0 1086 19 2262 887.3 0 2262 20 44426 887.3 0 44426 21 37683 887.3 0 37683 22 33169 887.3 0 33169 23 451 887.3 0 451 24 287 887.3 0 287 25 543 887.3 0 543 26 189 887.3 0 189 27 149 887.3 0 149 28 113 887.3 0 113 29 154 887.3 0 154 30 115 887.3 0 115 31 152 887.3 0 152 32 233 887.3 0 233 33 297 887.3 0 297 34 292 887.3 0 292 35 632 887.3 0 632 36 1575 887.3 0 1575 37 398 887.3 0 398 38 533 887.3 0 533 39 998 887.3 0 998 40 549 887.3 0 549 41 613 887.3 0 613 42 1018 887.3 0 1018 43 1116 887.3 0 1116 44 1412 887.3 0 1412 45 1262 887.3 0 1262 46 2199 887.3 0 2199 47 2761 887.3 0 2761 48 6480 887.3 0 6480 49 111497 887.3 0 111497 50 117221 887.3 0 117221 51 76304 887.3 0 76304 52 378 887.3 0 378 53 63 887.3 0 63 54 69 887.3 0 69 55 49 887.3 0 49 56 85 887.3 0 85 57 82 887.3 0 82 58 92 887.3 0 92 59 70 887.3 0 70 60 113 887.3 0 113 61 99 887.3 0 99 62 133 887.3 0 133 63 799 887.3 0 799 64 1085 887.3 0 1085 65 1104 887.3 0 1104 66 66 887.3 0 66 67 51 887.3 0 51 68 63 887.3 0 63 69 24 887.3 0 24 70 53 887.3 0 53 71 60 887.3 0 60 72 47 887.3 0 47 73 82 887.3 0 82 74 77 887.3 0 77 75 66 887.3 0 66 76 47 887.3 0 47 77 48 887.3 0 48 78 37 887.3 0 37 79 47 887.3 0 47 80 48 887.3 0 48 81 59 887.3 0 59 82 37 887.3 0 37 83 61 887.3 0 61 84 59 887.3 0 59 85 57 887.3 0 57 86 77 887.3 0 77 87 60 887.3 0 60 88 73 887.3 0 73 89 59 887.3 0 59 90 259 887.3 0 259 91 58 887.3 0 58 92 69 887.3 0 69 93 174 887.3 0 174 94 63 887.3 0 63 95 65 887.3 0 65 96 82 887.3 0 82 97 51 887.3 0 51 98 74 887.3 0 74 99 53 887.3 0 53 100 46 887.3 0 46 101 56 887.3 0 56 102 41 887.3 0 41 103 49 887.3 0 49 104 47 887.3 0 47 105 49 887.3 0 49 106 82 887.3 0 82 107 44 887.3 0 44 108 37 887.3 0 37 109 46 887.3 0 46 110 58 887.3 0 58 111 61 887.3 0 61 112 72 887.3 0 72 113 53 887.3 0 53 114 52 887.3 0 52 115 58 887.3 0 58 116 105 887.3 0 105 117 226 887.3 0 226 118 580 887.3 0 580 119 606 887.3 0 606 120 126 887.3 0 126 121 74 887.3 0 74 122 65 887.3 0 65 123 73 887.3 0 73 124 113 887.3 0 113 125 109 887.3 0 109 126 231 887.3 0 231 127 93 887.3 0 93 128 60 887.3 0 60 129 62 887.3 0 62 130 163 887.3 0 163 131 89 887.3 0 89 132 57 887.3 0 57 133 52 887.3 0 52 134 46 887.3 0 46 135 99 887.3 0 99 136 41 887.3 0 41 137 35 887.3 0 35 138 68 887.3 0 68 139 66 887.3 0 66 140 87 887.3 0 87 141 89 887.3 0 89 142 96 887.3 0 96 143 84 887.3 0 84 144 106 887.3 0 106 145 77 887.3 0 77 146 112 887.3 0 112 147 71 887.3 0 71 148 53 887.3 0 53 149 84 887.3 0 84 150 74 887.3 0 74 151 67 887.3 0 67 152 87 887.3 0 87 153 81 887.3 0 81 154 76 887.3 0 76 155 106 887.3 0 106 156 90 887.3 0 90 157 171 887.3 0 171 158 140 887.3 0 140 159 157 887.3 0 157 160 60 887.3 0 60 161 63 887.3 0 63 162 59 887.3 0 59 163 65 887.3 0 65 164 59 887.3 0 59 165 85 887.3 0 85 166 57 887.3 0 57 167 73 887.3 0 73 168 90 887.3 0 90 169 108 887.3 0 108 170 97 887.3 0 97 171 107 887.3 0 107 172 84 887.3 0 84 173 80 887.3 0 80 174 72 887.3 0 72 175 105 887.3 0 105 176 137 887.3 0 137 177 224 887.3 0 224 178 223 887.3 0 223 179 305 887.3 0 305 180 353 887.3 0 353 181 449 887.3 0 449 182 3398 887.3 0 3398 183 8460 887.3 0 8460 184 3500 887.3 0 3500 185 158 887.3 0 158 186 152 887.3 0 152 187 129 887.3 0 129 188 320 887.3 0 320 189 345 887.3 0 345 190 377 887.3 0 377 191 414 887.3 0 414 192 679 887.3 0 679 193 8981 887.3 0 8981 194 6918 887.3 0 6918 195 14448 887.3 0 14448 196 5172 887.3 0 5172 197 2994 887.3 0 2994 198 706 887.3 0 706 199 85 887.3 0 85 200 139 887.3 0 139 201 97 887.3 0 97 202 74 887.3 0 74 203 70 887.3 0 70 204 73 887.3 0 73 205 128 887.3 0 128 206 95 887.3 0 95 207 135 887.3 0 135 208 127 887.3 0 127 209 121 887.3 0 121 210 86 887.3 0 86 211 102 887.3 0 102 212 100 887.3 0 100 213 497 887.3 0 497 214 1131 887.3 0 1131 215 965 887.3 0 965 216 894 887.3 0 894 217 466 887.3 0 466 218 789 887.3 0 789 219 1564 887.3 0 1564 220 1162 887.3 0 1162 221 844 887.3 0 844 222 291 887.3 0 291 223 164 887.3 0 164 224 73 887.3 0 73 225 105 887.3 0 105 226 103 887.3 0 103 227 120 887.3 0 120 228 132 887.3 0 132 229 84 887.3 0 84 230 95 887.3 0 95 231 168 887.3 0 168 232 175 887.3 0 175 233 171 887.3 0 171 234 143 887.3 0 143 235 125 887.3 0 125 236 183 887.3 0 183 237 208 887.3 0 208 238 297 887.3 0 297 239 1128 887.3 0 1128 240 1898 887.3 0 1898 241 1129 887.3 0 1129 242 566 887.3 0 566 243 65 887.3 0 65 244 103 887.3 0 103 245 96 887.3 0 96 246 155 887.3 0 155 247 133 887.3 0 133 248 190 887.3 0 190 249 230 887.3 0 230 250 943 887.3 0 943 251 1358 887.3 0 1358 252 1622 887.3 0 1622 253 749 887.3 0 749 254 76 887.3 0 76 255 130 887.3 0 130 256 154 887.3 0 154 257 235 887.3 0 235 258 288 887.3 0 288 259 1256 887.3 0 1256 260 1053 887.3 0 1053 261 625 887.3 0 625 262 206 887.3 0 206 263 1305 887.3 0 1305 264 1298 887.3 0 1298 265 1657 887.3 0 1657 266 1160 887.3 0 1160 267 1468 887.3 0 1468 268 512 887.3 0 512 269 350 887.3 0 350 270 425 887.3 0 425 271 175 887.3 0 175 272 121 887.3 0 121 273 89 887.3 0 89 274 113 887.3 0 113 275 146 887.3 0 146 276 187 887.3 0 187 277 142 887.3 0 142 278 117 887.3 0 117 279 136 887.3 0 136 280 147 887.3 0 147 281 176 887.3 0 176 282 184 887.3 0 184 283 321 887.3 0 321 284 269 887.3 0 269 285 277 887.3 0 277 286 307 887.3 0 307 287 353 887.3 0 353 288 3139 887.3 0 3139 289 4208 887.3 0 4208 290 2680 887.3 0 2680 291 946 887.3 0 946 292 176 887.3 0 176 293 270 887.3 0 270 294 330 887.3 0 330 295 264 887.3 0 264 296 167 887.3 0 167 297 263 887.3 0 263 298 263 887.3 0 263 299 264 887.3 0 264 300 565 887.3 0 565 301 246 887.3 0 246 Finished in 45.85 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 643,519 Reads with adapters: 71,642 (11.1%) Reads that were too short: 316 (0.0%) Reads written (passing filters): 71,326 (11.1%) Total basepairs processed: 161,295,636 bp Total written (filtered): 17,686,331 bp (11.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 71642 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 27 0.0 3 0 0 21 2 4 19 704 0.0 3 0 619 49 24 12 20 53488 0.0 4 51197 1934 268 66 23 21 130 0.0 4 31 88 8 2 1 22 11 0.0 4 3 1 4 0 3 23 137 0.0 4 0 0 0 115 22 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 28 3 0.0 4 0 0 0 0 3 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 50 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 61 1 0.0 4 1 63 1 0.0 4 1 64 1 0.0 4 1 65 1 0.0 4 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 83 13 0.0 4 0 0 0 0 13 84 10 0.0 4 0 0 1 0 9 85 10596 0.0 4 0 0 0 1 10595 86 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 88 10 0.0 4 0 0 0 0 10 89 1 0.0 4 0 1 92 1 0.0 4 0 0 0 0 1 95 3 0.0 4 0 0 0 0 3 98 2 0.0 4 0 0 0 0 2 110 2 0.0 4 0 0 0 0 2 111 5 0.0 4 0 0 0 0 5 112 6 0.0 4 0 0 0 0 6 113 10 0.0 4 1 0 0 0 9 114 1739 0.0 4 0 0 0 0 1739 117 3 0.0 4 0 0 0 0 3 122 6 0.0 4 0 0 0 0 6 124 1 0.0 4 0 0 0 0 1 125 8 0.0 4 0 0 0 0 8 126 48 0.0 4 0 0 0 0 48 127 4292 0.0 4 0 0 0 0 4292 128 41 0.0 4 0 0 0 0 41 130 7 0.0 4 0 0 0 0 7 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 140 4 0.0 4 0 0 0 0 4 146 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 160 5 0.0 4 0 0 0 1 4 161 2 0.0 4 0 0 0 0 2 163 2 0.0 4 0 0 0 0 2 165 2 0.0 4 0 0 0 0 2 166 4 0.0 4 0 0 0 0 4 167 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 172 1 0.0 4 0 0 0 0 1 173 3 0.0 4 0 0 0 0 3 174 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 183 2 0.0 4 0 0 0 1 1 184 1 0.0 4 0 0 0 0 1 186 66 0.0 4 0 0 0 6 60 187 17 0.0 4 0 0 0 1 16 188 3 0.0 4 0 0 0 2 1 190 6 0.0 4 0 0 0 0 6 191 3 0.0 4 0 0 0 1 2 192 5 0.0 4 0 0 0 1 4 193 7 0.0 4 0 0 0 1 6 195 1 0.0 4 0 0 0 0 1 196 2 0.0 4 0 0 0 0 2 198 1 0.0 4 0 0 0 0 1 201 3 0.0 4 0 0 0 0 3 202 3 0.0 4 0 0 0 0 3 203 3 0.0 4 0 0 0 0 3 204 1 0.0 4 0 0 0 0 1 205 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 1 0 0 0 1 213 2 0.0 4 0 0 0 1 1 214 1 0.0 4 1 215 17 0.0 4 0 0 0 0 17 216 6 0.0 4 0 0 0 1 5 217 1 0.0 4 0 0 0 0 1 218 3 0.0 4 0 0 0 0 3 220 7 0.0 4 0 0 0 0 7 221 5 0.0 4 0 0 0 0 5 222 8 0.0 4 0 0 0 0 8 223 8 0.0 4 0 0 0 0 8 224 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 11 0.0 4 0 0 0 1 10 229 3 0.0 4 0 0 0 0 3 230 2 0.0 4 0 0 0 0 2 232 4 0.0 4 0 0 0 0 4 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 240 2 0.0 4 0 0 0 0 2 241 2 0.0 4 0 0 0 0 2 244 2 0.0 4 0 0 0 0 2 245 1 0.0 4 0 0 0 0 1 246 21 0.0 4 0 0 0 0 21 247 4 0.0 4 0 0 0 0 4 248 3 0.0 4 0 0 0 0 3 249 3 0.0 4 0 0 0 0 3 250 1 0.0 4 0 0 0 0 1 251 4 0.0 4 0 0 0 0 4 252 1 0.0 4 0 0 0 1 254 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 269 2 0.0 4 0 0 0 0 2 272 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 277 3 0.0 4 0 0 0 0 3 280 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 284 2 0.0 4 0 0 0 0 2 286 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.05 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 71,326 Reads with adapters: 12,178 (17.1%) Reads written (passing filters): 71,326 (100.0%) Total basepairs processed: 17,686,331 bp Total written (filtered): 17,548,332 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 12178 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 4.7% G: 91.0% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 169 1114.5 0 169 4 87 278.6 0 87 5 2 69.7 0 2 8 3 1.1 0 3 9 50 0.3 0 0 50 10 967 0.1 1 43 924 11 10779 0.0 1 158 10621 12 67 0.0 1 1 66 13 4 0.0 1 0 4 14 12 0.0 1 7 5 15 3 0.0 1 2 1 27 1 0.0 1 1 150 1 0.0 1 1 151 1 0.0 1 0 1 153 1 0.0 1 0 1 168 1 0.0 1 1 170 1 0.0 1 1 224 26 0.0 1 25 1 225 1 0.0 1 1 226 1 0.0 1 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.