This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 177.53 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 592,990 (16.3%) Reads that were too short: 23,123 (0.6%) Reads written (passing filters): 569,867 (15.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 142,721,722 bp (13.0%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 592990 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1580 887.3 0 1580 7 58122 887.3 0 58122 8 63935 887.3 0 63935 9 293 887.3 0 293 10 214 887.3 0 214 11 183 887.3 0 183 12 174 887.3 0 174 13 336 887.3 0 336 14 403 887.3 0 403 15 349 887.3 0 349 16 321 887.3 0 321 17 682 887.3 0 682 18 705 887.3 0 705 19 1470 887.3 0 1470 20 38007 887.3 0 38007 21 33494 887.3 0 33494 22 29736 887.3 0 29736 23 341 887.3 0 341 24 188 887.3 0 188 25 448 887.3 0 448 26 185 887.3 0 185 27 119 887.3 0 119 28 106 887.3 0 106 29 155 887.3 0 155 30 116 887.3 0 116 31 169 887.3 0 169 32 214 887.3 0 214 33 263 887.3 0 263 34 249 887.3 0 249 35 498 887.3 0 498 36 1304 887.3 0 1304 37 346 887.3 0 346 38 425 887.3 0 425 39 850 887.3 0 850 40 399 887.3 0 399 41 487 887.3 0 487 42 846 887.3 0 846 43 936 887.3 0 936 44 958 887.3 0 958 45 894 887.3 0 894 46 1665 887.3 0 1665 47 1820 887.3 0 1820 48 4258 887.3 0 4258 49 89943 887.3 0 89943 50 98217 887.3 0 98217 51 64401 887.3 0 64401 52 262 887.3 0 262 53 51 887.3 0 51 54 53 887.3 0 53 55 53 887.3 0 53 56 55 887.3 0 55 57 68 887.3 0 68 58 80 887.3 0 80 59 63 887.3 0 63 60 75 887.3 0 75 61 74 887.3 0 74 62 85 887.3 0 85 63 291 887.3 0 291 64 410 887.3 0 410 65 482 887.3 0 482 66 55 887.3 0 55 67 60 887.3 0 60 68 46 887.3 0 46 69 46 887.3 0 46 70 60 887.3 0 60 71 55 887.3 0 55 72 53 887.3 0 53 73 91 887.3 0 91 74 87 887.3 0 87 75 52 887.3 0 52 76 59 887.3 0 59 77 44 887.3 0 44 78 58 887.3 0 58 79 56 887.3 0 56 80 53 887.3 0 53 81 57 887.3 0 57 82 62 887.3 0 62 83 57 887.3 0 57 84 64 887.3 0 64 85 42 887.3 0 42 86 58 887.3 0 58 87 64 887.3 0 64 88 77 887.3 0 77 89 68 887.3 0 68 90 92 887.3 0 92 91 61 887.3 0 61 92 70 887.3 0 70 93 77 887.3 0 77 94 57 887.3 0 57 95 51 887.3 0 51 96 48 887.3 0 48 97 50 887.3 0 50 98 90 887.3 0 90 99 54 887.3 0 54 100 47 887.3 0 47 101 49 887.3 0 49 102 67 887.3 0 67 103 33 887.3 0 33 104 53 887.3 0 53 105 57 887.3 0 57 106 89 887.3 0 89 107 70 887.3 0 70 108 44 887.3 0 44 109 54 887.3 0 54 110 77 887.3 0 77 111 53 887.3 0 53 112 75 887.3 0 75 113 52 887.3 0 52 114 49 887.3 0 49 115 45 887.3 0 45 116 89 887.3 0 89 117 136 887.3 0 136 118 308 887.3 0 308 119 323 887.3 0 323 120 65 887.3 0 65 121 56 887.3 0 56 122 63 887.3 0 63 123 58 887.3 0 58 124 69 887.3 0 69 125 96 887.3 0 96 126 249 887.3 0 249 127 88 887.3 0 88 128 69 887.3 0 69 129 72 887.3 0 72 130 192 887.3 0 192 131 102 887.3 0 102 132 61 887.3 0 61 133 76 887.3 0 76 134 56 887.3 0 56 135 95 887.3 0 95 136 60 887.3 0 60 137 61 887.3 0 61 138 64 887.3 0 64 139 65 887.3 0 65 140 59 887.3 0 59 141 87 887.3 0 87 142 85 887.3 0 85 143 69 887.3 0 69 144 83 887.3 0 83 145 72 887.3 0 72 146 93 887.3 0 93 147 87 887.3 0 87 148 65 887.3 0 65 149 77 887.3 0 77 150 70 887.3 0 70 151 82 887.3 0 82 152 91 887.3 0 91 153 77 887.3 0 77 154 80 887.3 0 80 155 107 887.3 0 107 156 68 887.3 0 68 157 100 887.3 0 100 158 103 887.3 0 103 159 90 887.3 0 90 160 65 887.3 0 65 161 63 887.3 0 63 162 70 887.3 0 70 163 60 887.3 0 60 164 71 887.3 0 71 165 110 887.3 0 110 166 59 887.3 0 59 167 77 887.3 0 77 168 75 887.3 0 75 169 89 887.3 0 89 170 99 887.3 0 99 171 88 887.3 0 88 172 74 887.3 0 74 173 79 887.3 0 79 174 85 887.3 0 85 175 124 887.3 0 124 176 119 887.3 0 119 177 144 887.3 0 144 178 191 887.3 0 191 179 260 887.3 0 260 180 344 887.3 0 344 181 367 887.3 0 367 182 3855 887.3 0 3855 183 10566 887.3 0 10566 184 4428 887.3 0 4428 185 141 887.3 0 141 186 141 887.3 0 141 187 121 887.3 0 121 188 211 887.3 0 211 189 245 887.3 0 245 190 268 887.3 0 268 191 293 887.3 0 293 192 507 887.3 0 507 193 7491 887.3 0 7491 194 5982 887.3 0 5982 195 13532 887.3 0 13532 196 5142 887.3 0 5142 197 3148 887.3 0 3148 198 751 887.3 0 751 199 74 887.3 0 74 200 78 887.3 0 78 201 68 887.3 0 68 202 62 887.3 0 62 203 70 887.3 0 70 204 80 887.3 0 80 205 116 887.3 0 116 206 75 887.3 0 75 207 82 887.3 0 82 208 80 887.3 0 80 209 70 887.3 0 70 210 73 887.3 0 73 211 87 887.3 0 87 212 56 887.3 0 56 213 242 887.3 0 242 214 453 887.3 0 453 215 381 887.3 0 381 216 403 887.3 0 403 217 265 887.3 0 265 218 331 887.3 0 331 219 495 887.3 0 495 220 446 887.3 0 446 221 354 887.3 0 354 222 201 887.3 0 201 223 95 887.3 0 95 224 66 887.3 0 66 225 75 887.3 0 75 226 67 887.3 0 67 227 110 887.3 0 110 228 74 887.3 0 74 229 55 887.3 0 55 230 87 887.3 0 87 231 83 887.3 0 83 232 132 887.3 0 132 233 88 887.3 0 88 234 84 887.3 0 84 235 78 887.3 0 78 236 117 887.3 0 117 237 93 887.3 0 93 238 119 887.3 0 119 239 375 887.3 0 375 240 724 887.3 0 724 241 490 887.3 0 490 242 224 887.3 0 224 243 53 887.3 0 53 244 68 887.3 0 68 245 70 887.3 0 70 246 94 887.3 0 94 247 96 887.3 0 96 248 103 887.3 0 103 249 132 887.3 0 132 250 614 887.3 0 614 251 943 887.3 0 943 252 1007 887.3 0 1007 253 458 887.3 0 458 254 57 887.3 0 57 255 97 887.3 0 97 256 82 887.3 0 82 257 131 887.3 0 131 258 123 887.3 0 123 259 382 887.3 0 382 260 300 887.3 0 300 261 208 887.3 0 208 262 116 887.3 0 116 263 447 887.3 0 447 264 412 887.3 0 412 265 445 887.3 0 445 266 352 887.3 0 352 267 408 887.3 0 408 268 169 887.3 0 169 269 155 887.3 0 155 270 150 887.3 0 150 271 83 887.3 0 83 272 104 887.3 0 104 273 81 887.3 0 81 274 83 887.3 0 83 275 83 887.3 0 83 276 98 887.3 0 98 277 75 887.3 0 75 278 94 887.3 0 94 279 87 887.3 0 87 280 82 887.3 0 82 281 64 887.3 0 64 282 112 887.3 0 112 283 133 887.3 0 133 284 127 887.3 0 127 285 129 887.3 0 129 286 135 887.3 0 135 287 147 887.3 0 147 288 1197 887.3 0 1197 289 1625 887.3 0 1625 290 1297 887.3 0 1297 291 481 887.3 0 481 292 84 887.3 0 84 293 112 887.3 0 112 294 161 887.3 0 161 295 111 887.3 0 111 296 85 887.3 0 85 297 114 887.3 0 114 298 113 887.3 0 113 299 110 887.3 0 110 300 390 887.3 0 390 301 196 887.3 0 196 Finished in 36.43 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 569,867 Reads with adapters: 58,344 (10.2%) Reads that were too short: 439 (0.1%) Reads written (passing filters): 57,905 (10.2%) Total basepairs processed: 142,721,722 bp Total written (filtered): 15,740,502 bp (11.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58344 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 299 0.0 3 0 289 9 1 17 55387 0.0 3 54296 1056 32 3 18 157 0.0 3 10 140 6 1 19 22 0.0 3 0 0 19 3 20 48 0.0 3 0 0 0 48 21 1 0.0 3 0 0 0 1 24 1 0.0 3 1 28 2 0.0 3 2 29 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 34 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 10 0.0 3 0 0 0 10 49 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 1 3 54 2 0.0 3 1 0 0 1 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 60 1 0.0 3 1 63 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 73 96 0.0 3 0 0 0 96 74 8 0.0 3 0 0 0 8 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 5 0.0 3 0 0 0 5 91 9 0.0 3 0 0 0 9 92 6 0.0 3 0 0 1 5 93 14 0.0 3 0 0 8 6 96 1 0.0 3 0 0 0 1 97 36 0.0 3 0 0 0 36 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 102 18 0.0 3 0 0 1 17 103 6 0.0 3 0 0 0 6 104 4 0.0 3 0 0 0 4 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 3 0.0 3 0 0 0 3 120 2 0.0 3 0 0 0 2 122 460 0.0 3 0 0 2 458 123 190 0.0 3 0 0 2 188 124 47 0.0 3 0 0 0 47 125 88 0.0 3 0 0 1 87 126 32 0.0 3 0 0 1 31 127 15 0.0 3 0 0 1 14 128 31 0.0 3 0 0 0 31 129 4 0.0 3 0 0 0 4 130 8 0.0 3 0 0 0 8 131 23 0.0 3 0 0 0 23 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 10 0.0 3 0 0 0 10 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 13 0.0 3 0 0 0 13 138 7 0.0 3 0 0 0 7 139 8 0.0 3 0 0 0 8 140 4 0.0 3 0 0 0 4 141 12 0.0 3 0 0 0 12 142 142 0.0 3 0 0 0 142 143 13 0.0 3 0 0 0 13 144 1 0.0 3 0 0 0 1 145 14 0.0 3 0 0 0 14 146 18 0.0 3 0 0 0 18 147 2 0.0 3 0 0 0 2 148 14 0.0 3 0 0 0 14 149 9 0.0 3 0 0 0 9 150 1 0.0 3 0 0 0 1 151 113 0.0 3 0 0 1 112 152 21 0.0 3 0 0 0 21 153 11 0.0 3 0 0 0 11 154 24 0.0 3 0 0 0 24 155 3 0.0 3 0 0 0 3 156 2 0.0 3 0 0 0 2 157 3 0.0 3 0 0 1 2 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 3 0.0 3 1 0 0 2 162 1 0.0 3 0 0 0 1 163 5 0.0 3 0 0 0 5 164 2 0.0 3 0 0 0 2 165 4 0.0 3 0 0 0 4 166 7 0.0 3 1 0 0 6 167 2 0.0 3 0 0 0 2 168 3 0.0 3 0 0 0 3 170 72 0.0 3 0 0 1 71 171 104 0.0 3 0 0 0 104 172 206 0.0 3 0 0 0 206 173 4 0.0 3 1 0 0 3 174 19 0.0 3 0 0 0 19 175 14 0.0 3 0 0 0 14 177 8 0.0 3 0 0 0 8 179 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 188 1 0.0 3 0 0 0 1 189 4 0.0 3 0 0 0 4 191 1 0.0 3 0 0 0 1 193 9 0.0 3 0 0 2 7 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 197 3 0.0 3 0 0 0 3 199 1 0.0 3 0 0 0 1 202 8 0.0 3 0 0 1 7 203 3 0.0 3 0 0 0 3 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 2 0.0 3 1 0 0 1 217 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 3 0.0 3 0 0 0 3 223 2 0.0 3 0 0 0 2 224 2 0.0 3 0 0 1 1 227 2 0.0 3 0 0 0 2 231 8 0.0 3 0 0 0 8 232 3 0.0 3 0 0 0 3 234 27 0.0 3 0 0 0 27 235 19 0.0 3 0 0 0 19 236 13 0.0 3 0 0 0 13 237 16 0.0 3 0 0 0 16 238 38 0.0 3 0 0 1 37 239 9 0.0 3 0 0 1 8 240 3 0.0 3 0 0 0 3 241 3 0.0 3 0 0 1 2 242 3 0.0 3 0 0 0 3 243 16 0.0 3 0 0 0 16 244 21 0.0 3 0 0 0 21 245 4 0.0 3 0 0 0 4 246 3 0.0 3 0 0 0 3 247 5 0.0 3 0 0 0 5 248 1 0.0 3 0 0 0 1 250 10 0.0 3 0 0 0 10 251 4 0.0 3 0 0 0 4 252 24 0.0 3 0 0 0 24 254 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 256 1 0.0 3 1 258 2 0.0 3 0 0 0 2 262 4 0.0 3 0 0 0 4 263 5 0.0 3 0 0 0 5 264 4 0.0 3 0 0 0 4 265 3 0.0 3 0 0 0 3 266 5 0.0 3 0 0 0 5 268 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 4 0.0 3 0 0 0 4 273 8 0.0 3 0 0 1 7 274 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 2 0.0 3 0 0 0 2 281 5 0.0 3 0 0 0 5 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.29 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 57,905 Reads with adapters: 126 (0.2%) Reads written (passing filters): 57,905 (100.0%) Total basepairs processed: 15,740,502 bp Total written (filtered): 15,740,116 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 126 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.1% C: 23.0% G: 12.7% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 120 904.8 0 120 4 5 226.2 0 5 6 1 14.1 0 1