This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.47 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 184,192 (5.1%) Reads that were too short: 24,375 (0.7%) Reads written (passing filters): 159,817 (4.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 40,234,588 bp (3.7%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 184192 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 20549 887.3 0 20549 7 17631 887.3 0 17631 8 27506 887.3 0 27506 9 29495 887.3 0 29495 10 129 887.3 0 129 11 86 887.3 0 86 12 72 887.3 0 72 13 91 887.3 0 91 14 99 887.3 0 99 15 99 887.3 0 99 16 85 887.3 0 85 17 97 887.3 0 97 18 101 887.3 0 101 19 127 887.3 0 127 20 91 887.3 0 91 21 89 887.3 0 89 22 94 887.3 0 94 23 124 887.3 0 124 24 132 887.3 0 132 25 107 887.3 0 107 26 102 887.3 0 102 27 153 887.3 0 153 28 119 887.3 0 119 29 99 887.3 0 99 30 91 887.3 0 91 31 113 887.3 0 113 32 142 887.3 0 142 33 162 887.3 0 162 34 143 887.3 0 143 35 186 887.3 0 186 36 219 887.3 0 219 37 2614 887.3 0 2614 38 4046 887.3 0 4046 39 4251 887.3 0 4251 40 150 887.3 0 150 41 97 887.3 0 97 42 113 887.3 0 113 43 117 887.3 0 117 44 122 887.3 0 122 45 108 887.3 0 108 46 78 887.3 0 78 47 103 887.3 0 103 48 96 887.3 0 96 49 118 887.3 0 118 50 100 887.3 0 100 51 87 887.3 0 87 52 93 887.3 0 93 53 90 887.3 0 90 54 94 887.3 0 94 55 77 887.3 0 77 56 121 887.3 0 121 57 107 887.3 0 107 58 110 887.3 0 110 59 115 887.3 0 115 60 96 887.3 0 96 61 99 887.3 0 99 62 111 887.3 0 111 63 91 887.3 0 91 64 92 887.3 0 92 65 105 887.3 0 105 66 72 887.3 0 72 67 73 887.3 0 73 68 112 887.3 0 112 69 85 887.3 0 85 70 119 887.3 0 119 71 79 887.3 0 79 72 102 887.3 0 102 73 91 887.3 0 91 74 91 887.3 0 91 75 132 887.3 0 132 76 232 887.3 0 232 77 139 887.3 0 139 78 87 887.3 0 87 79 136 887.3 0 136 80 104 887.3 0 104 81 81 887.3 0 81 82 88 887.3 0 88 83 118 887.3 0 118 84 97 887.3 0 97 85 64 887.3 0 64 86 72 887.3 0 72 87 83 887.3 0 83 88 88 887.3 0 88 89 103 887.3 0 103 90 146 887.3 0 146 91 686 887.3 0 686 92 597 887.3 0 597 93 1159 887.3 0 1159 94 562 887.3 0 562 95 321 887.3 0 321 96 179 887.3 0 179 97 124 887.3 0 124 98 97 887.3 0 97 99 82 887.3 0 82 100 106 887.3 0 106 101 76 887.3 0 76 102 87 887.3 0 87 103 106 887.3 0 106 104 210 887.3 0 210 105 84 887.3 0 84 106 173 887.3 0 173 107 85 887.3 0 85 108 108 887.3 0 108 109 142 887.3 0 142 110 94 887.3 0 94 111 102 887.3 0 102 112 238 887.3 0 238 113 183 887.3 0 183 114 200 887.3 0 200 115 131 887.3 0 131 116 136 887.3 0 136 117 128 887.3 0 128 118 139 887.3 0 139 119 80 887.3 0 80 120 71 887.3 0 71 121 146 887.3 0 146 122 126 887.3 0 126 123 106 887.3 0 106 124 87 887.3 0 87 125 93 887.3 0 93 126 99 887.3 0 99 127 130 887.3 0 130 128 260 887.3 0 260 129 872 887.3 0 872 130 636 887.3 0 636 131 520 887.3 0 520 132 221 887.3 0 221 133 131 887.3 0 131 134 164 887.3 0 164 135 173 887.3 0 173 136 293 887.3 0 293 137 256 887.3 0 256 138 197 887.3 0 197 139 312 887.3 0 312 140 312 887.3 0 312 141 498 887.3 0 498 142 10319 887.3 0 10319 143 8156 887.3 0 8156 144 8842 887.3 0 8842 145 125 887.3 0 125 146 98 887.3 0 98 147 84 887.3 0 84 148 99 887.3 0 99 149 131 887.3 0 131 150 169 887.3 0 169 151 119 887.3 0 119 152 83 887.3 0 83 153 108 887.3 0 108 154 100 887.3 0 100 155 101 887.3 0 101 156 90 887.3 0 90 157 104 887.3 0 104 158 91 887.3 0 91 159 76 887.3 0 76 160 76 887.3 0 76 161 81 887.3 0 81 162 93 887.3 0 93 163 99 887.3 0 99 164 97 887.3 0 97 165 87 887.3 0 87 166 107 887.3 0 107 167 141 887.3 0 141 168 484 887.3 0 484 169 186 887.3 0 186 170 119 887.3 0 119 171 70 887.3 0 70 172 70 887.3 0 70 173 92 887.3 0 92 174 76 887.3 0 76 175 81 887.3 0 81 176 75 887.3 0 75 177 245 887.3 0 245 178 125 887.3 0 125 179 152 887.3 0 152 180 77 887.3 0 77 181 74 887.3 0 74 182 98 887.3 0 98 183 80 887.3 0 80 184 79 887.3 0 79 185 75 887.3 0 75 186 77 887.3 0 77 187 67 887.3 0 67 188 88 887.3 0 88 189 66 887.3 0 66 190 108 887.3 0 108 191 92 887.3 0 92 192 72 887.3 0 72 193 78 887.3 0 78 194 70 887.3 0 70 195 68 887.3 0 68 196 66 887.3 0 66 197 85 887.3 0 85 198 58 887.3 0 58 199 80 887.3 0 80 200 76 887.3 0 76 201 86 887.3 0 86 202 83 887.3 0 83 203 94 887.3 0 94 204 109 887.3 0 109 205 103 887.3 0 103 206 78 887.3 0 78 207 78 887.3 0 78 208 94 887.3 0 94 209 216 887.3 0 216 210 157 887.3 0 157 211 164 887.3 0 164 212 126 887.3 0 126 213 172 887.3 0 172 214 1759 887.3 0 1759 215 1541 887.3 0 1541 216 1775 887.3 0 1775 217 225 887.3 0 225 218 140 887.3 0 140 219 61 887.3 0 61 220 77 887.3 0 77 221 105 887.3 0 105 222 102 887.3 0 102 223 77 887.3 0 77 224 104 887.3 0 104 225 81 887.3 0 81 226 140 887.3 0 140 227 176 887.3 0 176 228 158 887.3 0 158 229 589 887.3 0 589 230 520 887.3 0 520 231 565 887.3 0 565 232 214 887.3 0 214 233 196 887.3 0 196 234 1312 887.3 0 1312 235 2579 887.3 0 2579 236 2416 887.3 0 2416 237 975 887.3 0 975 238 80 887.3 0 80 239 61 887.3 0 61 240 54 887.3 0 54 241 62 887.3 0 62 242 52 887.3 0 52 243 66 887.3 0 66 244 77 887.3 0 77 245 69 887.3 0 69 246 82 887.3 0 82 247 77 887.3 0 77 248 74 887.3 0 74 249 79 887.3 0 79 250 90 887.3 0 90 251 70 887.3 0 70 252 68 887.3 0 68 253 71 887.3 0 71 254 109 887.3 0 109 255 94 887.3 0 94 256 79 887.3 0 79 257 57 887.3 0 57 258 87 887.3 0 87 259 86 887.3 0 86 260 83 887.3 0 83 261 132 887.3 0 132 262 304 887.3 0 304 263 221 887.3 0 221 264 173 887.3 0 173 265 91 887.3 0 91 266 71 887.3 0 71 267 75 887.3 0 75 268 87 887.3 0 87 269 67 887.3 0 67 270 89 887.3 0 89 271 154 887.3 0 154 272 90 887.3 0 90 273 83 887.3 0 83 274 74 887.3 0 74 275 81 887.3 0 81 276 73 887.3 0 73 277 83 887.3 0 83 278 185 887.3 0 185 279 141 887.3 0 141 280 57 887.3 0 57 281 88 887.3 0 88 282 77 887.3 0 77 283 114 887.3 0 114 284 104 887.3 0 104 285 81 887.3 0 81 286 85 887.3 0 85 287 79 887.3 0 79 288 60 887.3 0 60 289 58 887.3 0 58 290 53 887.3 0 53 291 181 887.3 0 181 292 153 887.3 0 153 293 118 887.3 0 118 294 73 887.3 0 73 295 145 887.3 0 145 296 295 887.3 0 295 297 449 887.3 0 449 298 308 887.3 0 308 299 138 887.3 0 138 300 88 887.3 0 88 301 209 887.3 0 209 Finished in 10.91 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 159,817 Reads with adapters: 46,171 (28.9%) Reads that were too short: 84 (0.1%) Reads written (passing filters): 46,087 (28.8%) Total basepairs processed: 40,234,588 bp Total written (filtered): 12,732,801 bp (31.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 46171 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 6 1 16 127 0.0 3 0 123 4 17 27375 0.0 3 26656 694 23 2 18 18430 0.0 3 18024 397 9 19 57 0.0 3 12 34 10 1 20 25 0.0 3 5 0 5 15 21 15 0.0 3 0 1 0 14 22 2 0.0 3 1 0 0 1 26 1 0.0 3 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 8 0.0 3 0 0 0 8 53 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 74 3 0.0 3 3 76 2 0.0 3 2 77 1 0.0 3 0 1 79 1 0.0 3 0 0 0 1 83 3 0.0 3 0 0 0 3 85 1 0.0 3 1 87 2 0.0 3 1 0 0 1 97 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 0 4 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 4 0.0 3 0 0 0 4 136 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 165 1 0.0 3 1 176 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 206 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 218 55 0.0 3 0 0 3 52 222 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 263 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.62 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 46,087 Reads with adapters: 124 (0.3%) Reads written (passing filters): 46,087 (100.0%) Total basepairs processed: 12,732,801 bp Total written (filtered): 12,732,416 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 124 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 31.5% G: 14.5% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 112 720.1 0 112 4 11 180.0 0 11 5 1 45.0 0 1