This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 176.69 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 234,303 (6.4%) Reads that were too short: 16,703 (0.5%) Reads written (passing filters): 217,600 (6.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 60,303,311 bp (5.5%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 234303 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1206 887.3 0 1206 7 91507 887.3 0 91507 8 98817 887.3 0 98817 9 117 887.3 0 117 10 37 887.3 0 37 11 39 887.3 0 39 12 56 887.3 0 56 13 36 887.3 0 36 14 40 887.3 0 40 15 40 887.3 0 40 16 77 887.3 0 77 17 40 887.3 0 40 18 72 887.3 0 72 19 46 887.3 0 46 20 35 887.3 0 35 21 61 887.3 0 61 22 52 887.3 0 52 23 32 887.3 0 32 24 30 887.3 0 30 25 31 887.3 0 31 26 39 887.3 0 39 27 52 887.3 0 52 28 39 887.3 0 39 29 51 887.3 0 51 30 38 887.3 0 38 31 37 887.3 0 37 32 25 887.3 0 25 33 34 887.3 0 34 34 48 887.3 0 48 35 33 887.3 0 33 36 52 887.3 0 52 37 61 887.3 0 61 38 39 887.3 0 39 39 48 887.3 0 48 40 60 887.3 0 60 41 43 887.3 0 43 42 39 887.3 0 39 43 32 887.3 0 32 44 45 887.3 0 45 45 43 887.3 0 43 46 70 887.3 0 70 47 52 887.3 0 52 48 50 887.3 0 50 49 49 887.3 0 49 50 29 887.3 0 29 51 34 887.3 0 34 52 64 887.3 0 64 53 49 887.3 0 49 54 170 887.3 0 170 55 354 887.3 0 354 56 191 887.3 0 191 57 33 887.3 0 33 58 38 887.3 0 38 59 50 887.3 0 50 60 73 887.3 0 73 61 387 887.3 0 387 62 481 887.3 0 481 63 434 887.3 0 434 64 58 887.3 0 58 65 38 887.3 0 38 66 50 887.3 0 50 67 201 887.3 0 201 68 140 887.3 0 140 69 256 887.3 0 256 70 39 887.3 0 39 71 71 887.3 0 71 72 69 887.3 0 69 73 46 887.3 0 46 74 61 887.3 0 61 75 68 887.3 0 68 76 40 887.3 0 40 77 42 887.3 0 42 78 77 887.3 0 77 79 61 887.3 0 61 80 69 887.3 0 69 81 59 887.3 0 59 82 57 887.3 0 57 83 57 887.3 0 57 84 51 887.3 0 51 85 49 887.3 0 49 86 78 887.3 0 78 87 89 887.3 0 89 88 72 887.3 0 72 89 69 887.3 0 69 90 62 887.3 0 62 91 45 887.3 0 45 92 55 887.3 0 55 93 54 887.3 0 54 94 71 887.3 0 71 95 59 887.3 0 59 96 75 887.3 0 75 97 69 887.3 0 69 98 77 887.3 0 77 99 75 887.3 0 75 100 89 887.3 0 89 101 65 887.3 0 65 102 47 887.3 0 47 103 56 887.3 0 56 104 34 887.3 0 34 105 42 887.3 0 42 106 55 887.3 0 55 107 44 887.3 0 44 108 38 887.3 0 38 109 43 887.3 0 43 110 41 887.3 0 41 111 56 887.3 0 56 112 49 887.3 0 49 113 56 887.3 0 56 114 68 887.3 0 68 115 90 887.3 0 90 116 49 887.3 0 49 117 20 887.3 0 20 118 48 887.3 0 48 119 49 887.3 0 49 120 48 887.3 0 48 121 55 887.3 0 55 122 60 887.3 0 60 123 143 887.3 0 143 124 139 887.3 0 139 125 115 887.3 0 115 126 124 887.3 0 124 127 141 887.3 0 141 128 176 887.3 0 176 129 564 887.3 0 564 130 225 887.3 0 225 131 258 887.3 0 258 132 126 887.3 0 126 133 52 887.3 0 52 134 151 887.3 0 151 135 179 887.3 0 179 136 140 887.3 0 140 137 45 887.3 0 45 138 55 887.3 0 55 139 68 887.3 0 68 140 65 887.3 0 65 141 48 887.3 0 48 142 86 887.3 0 86 143 53 887.3 0 53 144 105 887.3 0 105 145 446 887.3 0 446 146 92 887.3 0 92 147 62 887.3 0 62 148 88 887.3 0 88 149 70 887.3 0 70 150 51 887.3 0 51 151 68 887.3 0 68 152 90 887.3 0 90 153 51 887.3 0 51 154 61 887.3 0 61 155 58 887.3 0 58 156 139 887.3 0 139 157 150 887.3 0 150 158 95 887.3 0 95 159 85 887.3 0 85 160 48 887.3 0 48 161 44 887.3 0 44 162 60 887.3 0 60 163 71 887.3 0 71 164 46 887.3 0 46 165 70 887.3 0 70 166 166 887.3 0 166 167 88 887.3 0 88 168 163 887.3 0 163 169 56 887.3 0 56 170 45 887.3 0 45 171 67 887.3 0 67 172 65 887.3 0 65 173 62 887.3 0 62 174 75 887.3 0 75 175 62 887.3 0 62 176 77 887.3 0 77 177 74 887.3 0 74 178 53 887.3 0 53 179 69 887.3 0 69 180 55 887.3 0 55 181 49 887.3 0 49 182 69 887.3 0 69 183 83 887.3 0 83 184 105 887.3 0 105 185 102 887.3 0 102 186 84 887.3 0 84 187 87 887.3 0 87 188 64 887.3 0 64 189 72 887.3 0 72 190 69 887.3 0 69 191 91 887.3 0 91 192 114 887.3 0 114 193 116 887.3 0 116 194 187 887.3 0 187 195 244 887.3 0 244 196 1183 887.3 0 1183 197 2319 887.3 0 2319 198 3300 887.3 0 3300 199 3143 887.3 0 3143 200 184 887.3 0 184 201 107 887.3 0 107 202 83 887.3 0 83 203 75 887.3 0 75 204 103 887.3 0 103 205 71 887.3 0 71 206 68 887.3 0 68 207 48 887.3 0 48 208 102 887.3 0 102 209 70 887.3 0 70 210 96 887.3 0 96 211 155 887.3 0 155 212 81 887.3 0 81 213 98 887.3 0 98 214 91 887.3 0 91 215 91 887.3 0 91 216 85 887.3 0 85 217 66 887.3 0 66 218 112 887.3 0 112 219 203 887.3 0 203 220 223 887.3 0 223 221 214 887.3 0 214 222 223 887.3 0 223 223 160 887.3 0 160 224 302 887.3 0 302 225 97 887.3 0 97 226 124 887.3 0 124 227 162 887.3 0 162 228 64 887.3 0 64 229 56 887.3 0 56 230 59 887.3 0 59 231 72 887.3 0 72 232 90 887.3 0 90 233 83 887.3 0 83 234 80 887.3 0 80 235 71 887.3 0 71 236 83 887.3 0 83 237 74 887.3 0 74 238 283 887.3 0 283 239 235 887.3 0 235 240 223 887.3 0 223 241 190 887.3 0 190 242 220 887.3 0 220 243 100 887.3 0 100 244 130 887.3 0 130 245 1122 887.3 0 1122 246 844 887.3 0 844 247 979 887.3 0 979 248 156 887.3 0 156 249 131 887.3 0 131 250 109 887.3 0 109 251 81 887.3 0 81 252 88 887.3 0 88 253 63 887.3 0 63 254 247 887.3 0 247 255 227 887.3 0 227 256 160 887.3 0 160 257 69 887.3 0 69 258 83 887.3 0 83 259 96 887.3 0 96 260 84 887.3 0 84 261 106 887.3 0 106 262 533 887.3 0 533 263 1841 887.3 0 1841 264 615 887.3 0 615 265 74 887.3 0 74 266 76 887.3 0 76 267 203 887.3 0 203 268 367 887.3 0 367 269 309 887.3 0 309 270 136 887.3 0 136 271 62 887.3 0 62 272 136 887.3 0 136 273 233 887.3 0 233 274 182 887.3 0 182 275 141 887.3 0 141 276 86 887.3 0 86 277 64 887.3 0 64 278 60 887.3 0 60 279 78 887.3 0 78 280 61 887.3 0 61 281 86 887.3 0 86 282 85 887.3 0 85 283 78 887.3 0 78 284 65 887.3 0 65 285 36 887.3 0 36 286 66 887.3 0 66 287 81 887.3 0 81 288 91 887.3 0 91 289 97 887.3 0 97 290 89 887.3 0 89 291 66 887.3 0 66 292 58 887.3 0 58 293 65 887.3 0 65 294 79 887.3 0 79 295 77 887.3 0 77 296 67 887.3 0 67 297 73 887.3 0 73 298 51 887.3 0 51 299 64 887.3 0 64 300 56 887.3 0 56 301 55 887.3 0 55 Finished in 15.97 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 217,600 Reads with adapters: 88,559 (40.7%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 88,531 (40.7%) Total basepairs processed: 60,303,311 bp Total written (filtered): 24,519,393 bp (40.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 88559 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 23 0.0 3 0 0 23 16 564 0.0 3 0 548 14 2 17 87648 0.0 3 86026 1571 46 5 18 166 0.0 3 5 158 2 1 19 41 0.0 3 1 0 33 7 20 55 0.0 3 0 0 0 55 22 1 0.0 3 0 0 1 24 1 0.0 3 1 25 1 0.0 3 1 27 1 0.0 3 1 28 1 0.0 3 0 1 29 1 0.0 3 1 32 1 0.0 3 0 0 0 1 39 1 0.0 3 1 42 6 0.0 3 0 0 0 6 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 55 2 0.0 3 1 0 0 1 58 1 0.0 3 0 0 0 1 63 5 0.0 3 0 0 0 5 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 72 1 0.0 3 1 80 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 190 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 227 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 249 2 0.0 3 0 0 0 2 271 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.23 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 88,531 Reads with adapters: 124 (0.1%) Reads written (passing filters): 88,531 (100.0%) Total basepairs processed: 24,519,393 bp Total written (filtered): 24,518,979 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 124 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.0% C: 33.9% G: 37.1% T: 8.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 104 1383.3 0 104 4 14 345.8 0 14 5 2 86.5 0 2 6 2 21.6 0 2 12 2 0.0 1 0 2