This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.71 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 95,530 (2.6%) Reads that were too short: 24,325 (0.7%) Reads written (passing filters): 71,205 (2.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 17,622,316 bp (1.6%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 95530 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1085 887.3 0 1085 7 20290 887.3 0 20290 8 506 887.3 0 506 9 23800 887.3 0 23800 10 62 887.3 0 62 11 13 887.3 0 13 12 20 887.3 0 20 13 19 887.3 0 19 14 32 887.3 0 32 15 21 887.3 0 21 16 30 887.3 0 30 17 14 887.3 0 14 18 8 887.3 0 8 19 6 887.3 0 6 20 18 887.3 0 18 21 16 887.3 0 16 22 10 887.3 0 10 23 33 887.3 0 33 24 17 887.3 0 17 25 12 887.3 0 12 26 16 887.3 0 16 27 14 887.3 0 14 28 15 887.3 0 15 29 9 887.3 0 9 30 13 887.3 0 13 31 11 887.3 0 11 32 12 887.3 0 12 33 25 887.3 0 25 34 16 887.3 0 16 35 19 887.3 0 19 36 19 887.3 0 19 37 19 887.3 0 19 38 40 887.3 0 40 39 21 887.3 0 21 40 32 887.3 0 32 41 136 887.3 0 136 42 41 887.3 0 41 43 57 887.3 0 57 44 10 887.3 0 10 45 19 887.3 0 19 46 18 887.3 0 18 47 31 887.3 0 31 48 16 887.3 0 16 49 18 887.3 0 18 50 24 887.3 0 24 51 42 887.3 0 42 52 30 887.3 0 30 53 24 887.3 0 24 54 19 887.3 0 19 55 22 887.3 0 22 56 32 887.3 0 32 57 24 887.3 0 24 58 20 887.3 0 20 59 23 887.3 0 23 60 23 887.3 0 23 61 23 887.3 0 23 62 19 887.3 0 19 63 19 887.3 0 19 64 16 887.3 0 16 65 21 887.3 0 21 66 21 887.3 0 21 67 26 887.3 0 26 68 26 887.3 0 26 69 153 887.3 0 153 70 314 887.3 0 314 71 127 887.3 0 127 72 32 887.3 0 32 73 26 887.3 0 26 74 33 887.3 0 33 75 29 887.3 0 29 76 40 887.3 0 40 77 59 887.3 0 59 78 36 887.3 0 36 79 23 887.3 0 23 80 68 887.3 0 68 81 53 887.3 0 53 82 47 887.3 0 47 83 32 887.3 0 32 84 36 887.3 0 36 85 43 887.3 0 43 86 49 887.3 0 49 87 135 887.3 0 135 88 56 887.3 0 56 89 120 887.3 0 120 90 183 887.3 0 183 91 108 887.3 0 108 92 78 887.3 0 78 93 67 887.3 0 67 94 67 887.3 0 67 95 63 887.3 0 63 96 98 887.3 0 98 97 123 887.3 0 123 98 110 887.3 0 110 99 89 887.3 0 89 100 1009 887.3 0 1009 101 1293 887.3 0 1293 102 2289 887.3 0 2289 103 447 887.3 0 447 104 129 887.3 0 129 105 44 887.3 0 44 106 44 887.3 0 44 107 49 887.3 0 49 108 22 887.3 0 22 109 31 887.3 0 31 110 46 887.3 0 46 111 46 887.3 0 46 112 42 887.3 0 42 113 63 887.3 0 63 114 44 887.3 0 44 115 45 887.3 0 45 116 56 887.3 0 56 117 46 887.3 0 46 118 124 887.3 0 124 119 77 887.3 0 77 120 51 887.3 0 51 121 53 887.3 0 53 122 104 887.3 0 104 123 140 887.3 0 140 124 141 887.3 0 141 125 83 887.3 0 83 126 62 887.3 0 62 127 45 887.3 0 45 128 43 887.3 0 43 129 66 887.3 0 66 130 77 887.3 0 77 131 170 887.3 0 170 132 431 887.3 0 431 133 298 887.3 0 298 134 115 887.3 0 115 135 83 887.3 0 83 136 77 887.3 0 77 137 181 887.3 0 181 138 159 887.3 0 159 139 142 887.3 0 142 140 203 887.3 0 203 141 113 887.3 0 113 142 136 887.3 0 136 143 228 887.3 0 228 144 350 887.3 0 350 145 313 887.3 0 313 146 108 887.3 0 108 147 73 887.3 0 73 148 89 887.3 0 89 149 121 887.3 0 121 150 122 887.3 0 122 151 91 887.3 0 91 152 124 887.3 0 124 153 106 887.3 0 106 154 90 887.3 0 90 155 78 887.3 0 78 156 114 887.3 0 114 157 396 887.3 0 396 158 786 887.3 0 786 159 365 887.3 0 365 160 479 887.3 0 479 161 335 887.3 0 335 162 419 887.3 0 419 163 116 887.3 0 116 164 68 887.3 0 68 165 74 887.3 0 74 166 63 887.3 0 63 167 97 887.3 0 97 168 172 887.3 0 172 169 167 887.3 0 167 170 130 887.3 0 130 171 84 887.3 0 84 172 87 887.3 0 87 173 93 887.3 0 93 174 77 887.3 0 77 175 100 887.3 0 100 176 903 887.3 0 903 177 1280 887.3 0 1280 178 1169 887.3 0 1169 179 169 887.3 0 169 180 135 887.3 0 135 181 109 887.3 0 109 182 148 887.3 0 148 183 162 887.3 0 162 184 154 887.3 0 154 185 133 887.3 0 133 186 98 887.3 0 98 187 202 887.3 0 202 188 161 887.3 0 161 189 226 887.3 0 226 190 136 887.3 0 136 191 109 887.3 0 109 192 77 887.3 0 77 193 74 887.3 0 74 194 103 887.3 0 103 195 87 887.3 0 87 196 191 887.3 0 191 197 209 887.3 0 209 198 211 887.3 0 211 199 111 887.3 0 111 200 109 887.3 0 109 201 115 887.3 0 115 202 173 887.3 0 173 203 229 887.3 0 229 204 177 887.3 0 177 205 148 887.3 0 148 206 116 887.3 0 116 207 108 887.3 0 108 208 95 887.3 0 95 209 91 887.3 0 91 210 143 887.3 0 143 211 257 887.3 0 257 212 231 887.3 0 231 213 161 887.3 0 161 214 153 887.3 0 153 215 163 887.3 0 163 216 138 887.3 0 138 217 164 887.3 0 164 218 158 887.3 0 158 219 168 887.3 0 168 220 144 887.3 0 144 221 149 887.3 0 149 222 276 887.3 0 276 223 438 887.3 0 438 224 484 887.3 0 484 225 267 887.3 0 267 226 183 887.3 0 183 227 156 887.3 0 156 228 147 887.3 0 147 229 147 887.3 0 147 230 100 887.3 0 100 231 99 887.3 0 99 232 103 887.3 0 103 233 145 887.3 0 145 234 134 887.3 0 134 235 151 887.3 0 151 236 126 887.3 0 126 237 133 887.3 0 133 238 337 887.3 0 337 239 364 887.3 0 364 240 319 887.3 0 319 241 198 887.3 0 198 242 272 887.3 0 272 243 426 887.3 0 426 244 373 887.3 0 373 245 317 887.3 0 317 246 219 887.3 0 219 247 234 887.3 0 234 248 212 887.3 0 212 249 150 887.3 0 150 250 159 887.3 0 159 251 189 887.3 0 189 252 158 887.3 0 158 253 191 887.3 0 191 254 198 887.3 0 198 255 226 887.3 0 226 256 300 887.3 0 300 257 311 887.3 0 311 258 247 887.3 0 247 259 194 887.3 0 194 260 183 887.3 0 183 261 137 887.3 0 137 262 285 887.3 0 285 263 433 887.3 0 433 264 613 887.3 0 613 265 548 887.3 0 548 266 432 887.3 0 432 267 256 887.3 0 256 268 141 887.3 0 141 269 164 887.3 0 164 270 259 887.3 0 259 271 486 887.3 0 486 272 467 887.3 0 467 273 344 887.3 0 344 274 326 887.3 0 326 275 258 887.3 0 258 276 313 887.3 0 313 277 280 887.3 0 280 278 267 887.3 0 267 279 371 887.3 0 371 280 196 887.3 0 196 281 174 887.3 0 174 282 211 887.3 0 211 283 280 887.3 0 280 284 285 887.3 0 285 285 279 887.3 0 279 286 289 887.3 0 289 287 324 887.3 0 324 288 289 887.3 0 289 289 216 887.3 0 216 290 246 887.3 0 246 291 287 887.3 0 287 292 439 887.3 0 439 293 304 887.3 0 304 294 290 887.3 0 290 295 141 887.3 0 141 296 139 887.3 0 139 297 169 887.3 0 169 298 242 887.3 0 242 299 291 887.3 0 291 300 386 887.3 0 386 301 436 887.3 0 436 Finished in 5.34 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 71,205 Reads with adapters: 21,516 (30.2%) Reads that were too short: 51 (0.1%) Reads written (passing filters): 21,465 (30.1%) Total basepairs processed: 17,622,316 bp Total written (filtered): 5,828,190 bp (33.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21516 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 8 0.0 3 0 0 8 19 216 0.0 3 0 187 13 8 8 20 20203 0.0 4 19233 787 142 34 7 21 49 0.0 4 6 41 2 22 9 0.0 4 0 6 0 0 3 23 495 0.0 4 444 20 2 19 10 24 1 0.0 4 0 1 28 1 0.0 4 1 34 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 84 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 9 0.0 4 0 0 0 0 9 127 461 0.0 4 0 0 0 0 461 130 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.22 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 21,465 Reads with adapters: 1,121 (5.2%) Reads written (passing filters): 21,465 (100.0%) Total basepairs processed: 5,828,190 bp Total written (filtered): 5,812,463 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1121 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.4% C: 8.5% G: 78.6% T: 2.1% none/other: 0.4% Overview of removed sequences length count expect max.err error counts 3 73 335.4 0 73 4 10 83.8 0 10 8 1 0.3 0 1 9 45 0.1 0 5 40 10 63 0.0 1 5 58 11 594 0.0 1 6 588 12 265 0.0 1 3 262 13 1 0.0 1 0 1 15 3 0.0 1 0 3 20 48 0.0 1 35 13 21 2 0.0 1 1 1 152 2 0.0 1 1 1 225 10 0.0 1 9 1 274 3 0.0 1 3 275 1 0.0 1 0 1