This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.58 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 76,272 (2.1%) Reads that were too short: 12,595 (0.3%) Reads written (passing filters): 63,677 (1.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 16,594,497 bp (1.5%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 76272 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1124 887.3 0 1124 7 26394 887.3 0 26394 8 378 887.3 0 378 9 19498 887.3 0 19498 10 36 887.3 0 36 11 19 887.3 0 19 12 16 887.3 0 16 13 21 887.3 0 21 14 13 887.3 0 13 15 142 887.3 0 142 16 15 887.3 0 15 17 20 887.3 0 20 18 11 887.3 0 11 19 7 887.3 0 7 20 20 887.3 0 20 21 14 887.3 0 14 22 22 887.3 0 22 23 26 887.3 0 26 24 16 887.3 0 16 25 13 887.3 0 13 26 17 887.3 0 17 27 8 887.3 0 8 28 25 887.3 0 25 29 24 887.3 0 24 30 11 887.3 0 11 31 29 887.3 0 29 32 27 887.3 0 27 33 27 887.3 0 27 34 37 887.3 0 37 35 38 887.3 0 38 36 24 887.3 0 24 37 18 887.3 0 18 38 24 887.3 0 24 39 28 887.3 0 28 40 42 887.3 0 42 41 26 887.3 0 26 42 47 887.3 0 47 43 23 887.3 0 23 44 24 887.3 0 24 45 27 887.3 0 27 46 27 887.3 0 27 47 24 887.3 0 24 48 17 887.3 0 17 49 28 887.3 0 28 50 35 887.3 0 35 51 62 887.3 0 62 52 24 887.3 0 24 53 27 887.3 0 27 54 22 887.3 0 22 55 20 887.3 0 20 56 14 887.3 0 14 57 41 887.3 0 41 58 25 887.3 0 25 59 23 887.3 0 23 60 19 887.3 0 19 61 18 887.3 0 18 62 13 887.3 0 13 63 30 887.3 0 30 64 15 887.3 0 15 65 29 887.3 0 29 66 28 887.3 0 28 67 26 887.3 0 26 68 25 887.3 0 25 69 135 887.3 0 135 70 259 887.3 0 259 71 102 887.3 0 102 72 17 887.3 0 17 73 16 887.3 0 16 74 24 887.3 0 24 75 16 887.3 0 16 76 30 887.3 0 30 77 22 887.3 0 22 78 19 887.3 0 19 79 27 887.3 0 27 80 26 887.3 0 26 81 39 887.3 0 39 82 35 887.3 0 35 83 27 887.3 0 27 84 34 887.3 0 34 85 33 887.3 0 33 86 42 887.3 0 42 87 84 887.3 0 84 88 42 887.3 0 42 89 63 887.3 0 63 90 73 887.3 0 73 91 69 887.3 0 69 92 47 887.3 0 47 93 44 887.3 0 44 94 57 887.3 0 57 95 41 887.3 0 41 96 66 887.3 0 66 97 87 887.3 0 87 98 68 887.3 0 68 99 50 887.3 0 50 100 314 887.3 0 314 101 391 887.3 0 391 102 887 887.3 0 887 103 258 887.3 0 258 104 99 887.3 0 99 105 56 887.3 0 56 106 40 887.3 0 40 107 41 887.3 0 41 108 29 887.3 0 29 109 35 887.3 0 35 110 33 887.3 0 33 111 46 887.3 0 46 112 37 887.3 0 37 113 44 887.3 0 44 114 25 887.3 0 25 115 23 887.3 0 23 116 43 887.3 0 43 117 35 887.3 0 35 118 67 887.3 0 67 119 42 887.3 0 42 120 36 887.3 0 36 121 22 887.3 0 22 122 62 887.3 0 62 123 90 887.3 0 90 124 55 887.3 0 55 125 46 887.3 0 46 126 37 887.3 0 37 127 32 887.3 0 32 128 21 887.3 0 21 129 56 887.3 0 56 130 37 887.3 0 37 131 92 887.3 0 92 132 222 887.3 0 222 133 199 887.3 0 199 134 76 887.3 0 76 135 65 887.3 0 65 136 52 887.3 0 52 137 81 887.3 0 81 138 69 887.3 0 69 139 74 887.3 0 74 140 145 887.3 0 145 141 72 887.3 0 72 142 79 887.3 0 79 143 120 887.3 0 120 144 142 887.3 0 142 145 139 887.3 0 139 146 51 887.3 0 51 147 55 887.3 0 55 148 80 887.3 0 80 149 97 887.3 0 97 150 73 887.3 0 73 151 67 887.3 0 67 152 84 887.3 0 84 153 70 887.3 0 70 154 51 887.3 0 51 155 77 887.3 0 77 156 60 887.3 0 60 157 222 887.3 0 222 158 535 887.3 0 535 159 238 887.3 0 238 160 344 887.3 0 344 161 171 887.3 0 171 162 227 887.3 0 227 163 64 887.3 0 64 164 66 887.3 0 66 165 42 887.3 0 42 166 45 887.3 0 45 167 46 887.3 0 46 168 104 887.3 0 104 169 74 887.3 0 74 170 86 887.3 0 86 171 63 887.3 0 63 172 54 887.3 0 54 173 69 887.3 0 69 174 45 887.3 0 45 175 68 887.3 0 68 176 787 887.3 0 787 177 1246 887.3 0 1246 178 1270 887.3 0 1270 179 81 887.3 0 81 180 74 887.3 0 74 181 53 887.3 0 53 182 59 887.3 0 59 183 79 887.3 0 79 184 92 887.3 0 92 185 77 887.3 0 77 186 68 887.3 0 68 187 136 887.3 0 136 188 122 887.3 0 122 189 114 887.3 0 114 190 91 887.3 0 91 191 64 887.3 0 64 192 48 887.3 0 48 193 26 887.3 0 26 194 53 887.3 0 53 195 50 887.3 0 50 196 89 887.3 0 89 197 101 887.3 0 101 198 94 887.3 0 94 199 76 887.3 0 76 200 60 887.3 0 60 201 56 887.3 0 56 202 72 887.3 0 72 203 78 887.3 0 78 204 66 887.3 0 66 205 69 887.3 0 69 206 67 887.3 0 67 207 62 887.3 0 62 208 67 887.3 0 67 209 53 887.3 0 53 210 96 887.3 0 96 211 98 887.3 0 98 212 110 887.3 0 110 213 108 887.3 0 108 214 88 887.3 0 88 215 84 887.3 0 84 216 83 887.3 0 83 217 86 887.3 0 86 218 70 887.3 0 70 219 71 887.3 0 71 220 82 887.3 0 82 221 73 887.3 0 73 222 99 887.3 0 99 223 123 887.3 0 123 224 177 887.3 0 177 225 126 887.3 0 126 226 101 887.3 0 101 227 91 887.3 0 91 228 80 887.3 0 80 229 67 887.3 0 67 230 107 887.3 0 107 231 81 887.3 0 81 232 86 887.3 0 86 233 91 887.3 0 91 234 78 887.3 0 78 235 89 887.3 0 89 236 73 887.3 0 73 237 117 887.3 0 117 238 284 887.3 0 284 239 229 887.3 0 229 240 205 887.3 0 205 241 110 887.3 0 110 242 112 887.3 0 112 243 165 887.3 0 165 244 164 887.3 0 164 245 106 887.3 0 106 246 103 887.3 0 103 247 144 887.3 0 144 248 106 887.3 0 106 249 106 887.3 0 106 250 99 887.3 0 99 251 128 887.3 0 128 252 103 887.3 0 103 253 105 887.3 0 105 254 108 887.3 0 108 255 114 887.3 0 114 256 126 887.3 0 126 257 151 887.3 0 151 258 108 887.3 0 108 259 121 887.3 0 121 260 100 887.3 0 100 261 96 887.3 0 96 262 202 887.3 0 202 263 215 887.3 0 215 264 334 887.3 0 334 265 234 887.3 0 234 266 209 887.3 0 209 267 120 887.3 0 120 268 68 887.3 0 68 269 96 887.3 0 96 270 110 887.3 0 110 271 170 887.3 0 170 272 163 887.3 0 163 273 144 887.3 0 144 274 190 887.3 0 190 275 185 887.3 0 185 276 181 887.3 0 181 277 156 887.3 0 156 278 119 887.3 0 119 279 162 887.3 0 162 280 93 887.3 0 93 281 114 887.3 0 114 282 142 887.3 0 142 283 147 887.3 0 147 284 149 887.3 0 149 285 128 887.3 0 128 286 146 887.3 0 146 287 159 887.3 0 159 288 153 887.3 0 153 289 118 887.3 0 118 290 127 887.3 0 127 291 152 887.3 0 152 292 175 887.3 0 175 293 155 887.3 0 155 294 118 887.3 0 118 295 106 887.3 0 106 296 96 887.3 0 96 297 124 887.3 0 124 298 154 887.3 0 154 299 175 887.3 0 175 300 197 887.3 0 197 301 247 887.3 0 247 Finished in 4.53 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 63,677 Reads with adapters: 20,016 (31.4%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 20,000 (31.4%) Total basepairs processed: 16,594,497 bp Total written (filtered): 5,494,016 bp (33.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20016 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 15 0.0 3 0 0 15 16 178 0.0 3 0 173 4 1 17 19663 0.0 3 19231 411 19 2 18 44 0.0 3 1 43 19 20 0.0 3 4 0 12 4 20 30 0.0 3 24 0 0 6 22 2 0.0 3 0 0 0 2 25 1 0.0 3 1 34 5 0.0 3 0 0 0 5 35 3 0.0 3 1 0 0 2 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 71 17 0.0 3 0 0 0 17 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 158 1 0.0 3 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 262 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.16 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 20,000 Reads with adapters: 336 (1.7%) Reads written (passing filters): 20,000 (100.0%) Total basepairs processed: 5,494,016 bp Total written (filtered): 5,492,989 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 336 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 82.4% C: 7.7% G: 8.3% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 325 312.5 0 325 4 10 78.1 0 10 12 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.