This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 172.65 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 140,828 (3.9%) Reads that were too short: 67,869 (1.9%) Reads written (passing filters): 72,959 (2.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 19,398,577 bp (1.8%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 140828 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2022 887.3 0 2022 7 800 887.3 0 800 8 28202 887.3 0 28202 9 23401 887.3 0 23401 10 72 887.3 0 72 11 59 887.3 0 59 12 96 887.3 0 96 13 114 887.3 0 114 14 62 887.3 0 62 15 50 887.3 0 50 16 57 887.3 0 57 17 85 887.3 0 85 18 61 887.3 0 61 19 104 887.3 0 104 20 63 887.3 0 63 21 74 887.3 0 74 22 68 887.3 0 68 23 72 887.3 0 72 24 91 887.3 0 91 25 53 887.3 0 53 26 43 887.3 0 43 27 53 887.3 0 53 28 73 887.3 0 73 29 65 887.3 0 65 30 47 887.3 0 47 31 62 887.3 0 62 32 67 887.3 0 67 33 58 887.3 0 58 34 70 887.3 0 70 35 57 887.3 0 57 36 76 887.3 0 76 37 58 887.3 0 58 38 71 887.3 0 71 39 69 887.3 0 69 40 113 887.3 0 113 41 110 887.3 0 110 42 61 887.3 0 61 43 65 887.3 0 65 44 72 887.3 0 72 45 67 887.3 0 67 46 62 887.3 0 62 47 62 887.3 0 62 48 45 887.3 0 45 49 48 887.3 0 48 50 62 887.3 0 62 51 111 887.3 0 111 52 141 887.3 0 141 53 172 887.3 0 172 54 74 887.3 0 74 55 53 887.3 0 53 56 53 887.3 0 53 57 51 887.3 0 51 58 75 887.3 0 75 59 90 887.3 0 90 60 60 887.3 0 60 61 61 887.3 0 61 62 89 887.3 0 89 63 124 887.3 0 124 64 65 887.3 0 65 65 55 887.3 0 55 66 105 887.3 0 105 67 71 887.3 0 71 68 72 887.3 0 72 69 60 887.3 0 60 70 50 887.3 0 50 71 63 887.3 0 63 72 66 887.3 0 66 73 59 887.3 0 59 74 74 887.3 0 74 75 88 887.3 0 88 76 76 887.3 0 76 77 73 887.3 0 73 78 126 887.3 0 126 79 73 887.3 0 73 80 61 887.3 0 61 81 80 887.3 0 80 82 82 887.3 0 82 83 76 887.3 0 76 84 84 887.3 0 84 85 66 887.3 0 66 86 79 887.3 0 79 87 64 887.3 0 64 88 69 887.3 0 69 89 98 887.3 0 98 90 85 887.3 0 85 91 119 887.3 0 119 92 91 887.3 0 91 93 95 887.3 0 95 94 113 887.3 0 113 95 111 887.3 0 111 96 73 887.3 0 73 97 99 887.3 0 99 98 73 887.3 0 73 99 100 887.3 0 100 100 142 887.3 0 142 101 110 887.3 0 110 102 93 887.3 0 93 103 79 887.3 0 79 104 55 887.3 0 55 105 63 887.3 0 63 106 59 887.3 0 59 107 81 887.3 0 81 108 69 887.3 0 69 109 89 887.3 0 89 110 71 887.3 0 71 111 71 887.3 0 71 112 97 887.3 0 97 113 71 887.3 0 71 114 77 887.3 0 77 115 69 887.3 0 69 116 94 887.3 0 94 117 83 887.3 0 83 118 102 887.3 0 102 119 66 887.3 0 66 120 64 887.3 0 64 121 78 887.3 0 78 122 92 887.3 0 92 123 81 887.3 0 81 124 183 887.3 0 183 125 92 887.3 0 92 126 83 887.3 0 83 127 67 887.3 0 67 128 69 887.3 0 69 129 68 887.3 0 68 130 66 887.3 0 66 131 94 887.3 0 94 132 114 887.3 0 114 133 130 887.3 0 130 134 74 887.3 0 74 135 80 887.3 0 80 136 132 887.3 0 132 137 479 887.3 0 479 138 1288 887.3 0 1288 139 974 887.3 0 974 140 595 887.3 0 595 141 67 887.3 0 67 142 79 887.3 0 79 143 82 887.3 0 82 144 130 887.3 0 130 145 84 887.3 0 84 146 91 887.3 0 91 147 76 887.3 0 76 148 70 887.3 0 70 149 66 887.3 0 66 150 77 887.3 0 77 151 84 887.3 0 84 152 78 887.3 0 78 153 91 887.3 0 91 154 83 887.3 0 83 155 85 887.3 0 85 156 66 887.3 0 66 157 78 887.3 0 78 158 85 887.3 0 85 159 81 887.3 0 81 160 81 887.3 0 81 161 75 887.3 0 75 162 72 887.3 0 72 163 72 887.3 0 72 164 74 887.3 0 74 165 87 887.3 0 87 166 79 887.3 0 79 167 79 887.3 0 79 168 73 887.3 0 73 169 86 887.3 0 86 170 74 887.3 0 74 171 104 887.3 0 104 172 86 887.3 0 86 173 113 887.3 0 113 174 72 887.3 0 72 175 61 887.3 0 61 176 70 887.3 0 70 177 77 887.3 0 77 178 76 887.3 0 76 179 100 887.3 0 100 180 107 887.3 0 107 181 103 887.3 0 103 182 84 887.3 0 84 183 103 887.3 0 103 184 85 887.3 0 85 185 77 887.3 0 77 186 122 887.3 0 122 187 121 887.3 0 121 188 119 887.3 0 119 189 115 887.3 0 115 190 64 887.3 0 64 191 80 887.3 0 80 192 83 887.3 0 83 193 54 887.3 0 54 194 64 887.3 0 64 195 76 887.3 0 76 196 84 887.3 0 84 197 77 887.3 0 77 198 101 887.3 0 101 199 86 887.3 0 86 200 85 887.3 0 85 201 71 887.3 0 71 202 85 887.3 0 85 203 104 887.3 0 104 204 109 887.3 0 109 205 78 887.3 0 78 206 70 887.3 0 70 207 92 887.3 0 92 208 98 887.3 0 98 209 77 887.3 0 77 210 113 887.3 0 113 211 79 887.3 0 79 212 83 887.3 0 83 213 74 887.3 0 74 214 177 887.3 0 177 215 149 887.3 0 149 216 119 887.3 0 119 217 102 887.3 0 102 218 88 887.3 0 88 219 95 887.3 0 95 220 84 887.3 0 84 221 61 887.3 0 61 222 65 887.3 0 65 223 81 887.3 0 81 224 89 887.3 0 89 225 110 887.3 0 110 226 73 887.3 0 73 227 85 887.3 0 85 228 85 887.3 0 85 229 90 887.3 0 90 230 106 887.3 0 106 231 77 887.3 0 77 232 68 887.3 0 68 233 84 887.3 0 84 234 86 887.3 0 86 235 95 887.3 0 95 236 93 887.3 0 93 237 99 887.3 0 99 238 316 887.3 0 316 239 379 887.3 0 379 240 226 887.3 0 226 241 68 887.3 0 68 242 92 887.3 0 92 243 103 887.3 0 103 244 80 887.3 0 80 245 83 887.3 0 83 246 72 887.3 0 72 247 99 887.3 0 99 248 75 887.3 0 75 249 92 887.3 0 92 250 106 887.3 0 106 251 121 887.3 0 121 252 114 887.3 0 114 253 76 887.3 0 76 254 77 887.3 0 77 255 123 887.3 0 123 256 138 887.3 0 138 257 233 887.3 0 233 258 258 887.3 0 258 259 320 887.3 0 320 260 245 887.3 0 245 261 150 887.3 0 150 262 124 887.3 0 124 263 174 887.3 0 174 264 163 887.3 0 163 265 120 887.3 0 120 266 168 887.3 0 168 267 132 887.3 0 132 268 148 887.3 0 148 269 147 887.3 0 147 270 215 887.3 0 215 271 291 887.3 0 291 272 572 887.3 0 572 273 6482 887.3 0 6482 274 5895 887.3 0 5895 275 3416 887.3 0 3416 276 441 887.3 0 441 277 766 887.3 0 766 278 304 887.3 0 304 279 221 887.3 0 221 280 204 887.3 0 204 281 133 887.3 0 133 282 184 887.3 0 184 283 517 887.3 0 517 284 687 887.3 0 687 285 965 887.3 0 965 286 1821 887.3 0 1821 287 1643 887.3 0 1643 288 4193 887.3 0 4193 289 355 887.3 0 355 290 232 887.3 0 232 291 296 887.3 0 296 292 482 887.3 0 482 293 3418 887.3 0 3418 294 2425 887.3 0 2425 295 1253 887.3 0 1253 296 290 887.3 0 290 297 823 887.3 0 823 298 3151 887.3 0 3151 299 7888 887.3 0 7888 300 6350 887.3 0 6350 301 3811 887.3 0 3811 Finished in 5.80 s (79 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 72,959 Reads with adapters: 26,142 (35.8%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 26,120 (35.8%) Total basepairs processed: 19,398,577 bp Total written (filtered): 7,073,525 bp (36.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26142 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 19 0.0 3 0 0 14 3 2 19 352 0.0 3 0 331 16 5 20 23866 0.0 4 22820 869 134 32 11 21 74 0.0 4 10 55 4 3 2 22 1435 0.0 4 1363 53 18 1 23 41 0.0 4 3 3 1 23 11 24 2 0.0 4 0 0 1 0 1 25 4 0.0 4 1 0 1 1 1 26 1 0.0 4 1 29 1 0.0 4 1 31 1 0.0 4 1 35 2 0.0 4 0 0 0 0 2 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 74 1 0.0 4 0 0 0 0 1 78 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 94 3 0.0 4 0 0 0 0 3 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 300 0.0 4 0 0 0 0 300 128 4 0.0 4 0 0 0 0 4 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 1 1 234 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.61 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 26,120 Reads with adapters: 5,465 (20.9%) Reads written (passing filters): 26,120 (100.0%) Total basepairs processed: 7,073,525 bp Total written (filtered): 7,016,974 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5465 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 4.9% G: 92.2% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 408.1 0 14 4 5 102.0 0 5 5 3 25.5 0 3 6 1 6.4 0 1 8 1 0.4 0 1 9 958 0.1 0 43 915 10 3720 0.0 1 170 3550 11 737 0.0 1 12 725 12 5 0.0 1 0 5 13 9 0.0 1 0 9 14 1 0.0 1 0 1 122 1 0.0 1 1 208 1 0.0 1 0 1 223 7 0.0 1 6 1 224 1 0.0 1 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.