This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 171.78 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 133,898 (3.7%) Reads that were too short: 64,473 (1.8%) Reads written (passing filters): 69,425 (1.9%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,943,005 bp (1.7%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 133898 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2055 887.3 0 2055 7 617 887.3 0 617 8 25180 887.3 0 25180 9 28571 887.3 0 28571 10 55 887.3 0 55 11 35 887.3 0 35 12 59 887.3 0 59 13 71 887.3 0 71 14 47 887.3 0 47 15 38 887.3 0 38 16 56 887.3 0 56 17 55 887.3 0 55 18 39 887.3 0 39 19 68 887.3 0 68 20 50 887.3 0 50 21 45 887.3 0 45 22 48 887.3 0 48 23 55 887.3 0 55 24 56 887.3 0 56 25 44 887.3 0 44 26 37 887.3 0 37 27 51 887.3 0 51 28 57 887.3 0 57 29 49 887.3 0 49 30 49 887.3 0 49 31 55 887.3 0 55 32 40 887.3 0 40 33 46 887.3 0 46 34 55 887.3 0 55 35 48 887.3 0 48 36 78 887.3 0 78 37 44 887.3 0 44 38 42 887.3 0 42 39 35 887.3 0 35 40 69 887.3 0 69 41 47 887.3 0 47 42 47 887.3 0 47 43 50 887.3 0 50 44 68 887.3 0 68 45 47 887.3 0 47 46 69 887.3 0 69 47 44 887.3 0 44 48 38 887.3 0 38 49 27 887.3 0 27 50 39 887.3 0 39 51 46 887.3 0 46 52 60 887.3 0 60 53 72 887.3 0 72 54 57 887.3 0 57 55 55 887.3 0 55 56 49 887.3 0 49 57 40 887.3 0 40 58 63 887.3 0 63 59 54 887.3 0 54 60 52 887.3 0 52 61 65 887.3 0 65 62 67 887.3 0 67 63 99 887.3 0 99 64 55 887.3 0 55 65 48 887.3 0 48 66 105 887.3 0 105 67 91 887.3 0 91 68 66 887.3 0 66 69 53 887.3 0 53 70 46 887.3 0 46 71 51 887.3 0 51 72 61 887.3 0 61 73 71 887.3 0 71 74 55 887.3 0 55 75 72 887.3 0 72 76 49 887.3 0 49 77 59 887.3 0 59 78 57 887.3 0 57 79 43 887.3 0 43 80 50 887.3 0 50 81 56 887.3 0 56 82 47 887.3 0 47 83 38 887.3 0 38 84 57 887.3 0 57 85 53 887.3 0 53 86 54 887.3 0 54 87 52 887.3 0 52 88 43 887.3 0 43 89 50 887.3 0 50 90 54 887.3 0 54 91 52 887.3 0 52 92 76 887.3 0 76 93 84 887.3 0 84 94 69 887.3 0 69 95 70 887.3 0 70 96 55 887.3 0 55 97 49 887.3 0 49 98 68 887.3 0 68 99 66 887.3 0 66 100 128 887.3 0 128 101 74 887.3 0 74 102 76 887.3 0 76 103 68 887.3 0 68 104 55 887.3 0 55 105 71 887.3 0 71 106 61 887.3 0 61 107 57 887.3 0 57 108 51 887.3 0 51 109 79 887.3 0 79 110 65 887.3 0 65 111 54 887.3 0 54 112 55 887.3 0 55 113 56 887.3 0 56 114 61 887.3 0 61 115 62 887.3 0 62 116 56 887.3 0 56 117 45 887.3 0 45 118 64 887.3 0 64 119 56 887.3 0 56 120 45 887.3 0 45 121 67 887.3 0 67 122 60 887.3 0 60 123 60 887.3 0 60 124 118 887.3 0 118 125 53 887.3 0 53 126 52 887.3 0 52 127 58 887.3 0 58 128 51 887.3 0 51 129 44 887.3 0 44 130 63 887.3 0 63 131 73 887.3 0 73 132 72 887.3 0 72 133 125 887.3 0 125 134 68 887.3 0 68 135 54 887.3 0 54 136 65 887.3 0 65 137 209 887.3 0 209 138 535 887.3 0 535 139 481 887.3 0 481 140 355 887.3 0 355 141 56 887.3 0 56 142 62 887.3 0 62 143 59 887.3 0 59 144 85 887.3 0 85 145 65 887.3 0 65 146 63 887.3 0 63 147 45 887.3 0 45 148 62 887.3 0 62 149 80 887.3 0 80 150 61 887.3 0 61 151 70 887.3 0 70 152 64 887.3 0 64 153 47 887.3 0 47 154 67 887.3 0 67 155 51 887.3 0 51 156 73 887.3 0 73 157 62 887.3 0 62 158 57 887.3 0 57 159 57 887.3 0 57 160 75 887.3 0 75 161 51 887.3 0 51 162 70 887.3 0 70 163 58 887.3 0 58 164 49 887.3 0 49 165 67 887.3 0 67 166 55 887.3 0 55 167 44 887.3 0 44 168 68 887.3 0 68 169 56 887.3 0 56 170 77 887.3 0 77 171 68 887.3 0 68 172 76 887.3 0 76 173 71 887.3 0 71 174 62 887.3 0 62 175 45 887.3 0 45 176 50 887.3 0 50 177 47 887.3 0 47 178 75 887.3 0 75 179 72 887.3 0 72 180 94 887.3 0 94 181 71 887.3 0 71 182 63 887.3 0 63 183 73 887.3 0 73 184 66 887.3 0 66 185 83 887.3 0 83 186 91 887.3 0 91 187 100 887.3 0 100 188 79 887.3 0 79 189 76 887.3 0 76 190 58 887.3 0 58 191 56 887.3 0 56 192 66 887.3 0 66 193 67 887.3 0 67 194 66 887.3 0 66 195 58 887.3 0 58 196 76 887.3 0 76 197 78 887.3 0 78 198 84 887.3 0 84 199 77 887.3 0 77 200 65 887.3 0 65 201 75 887.3 0 75 202 71 887.3 0 71 203 74 887.3 0 74 204 58 887.3 0 58 205 64 887.3 0 64 206 66 887.3 0 66 207 63 887.3 0 63 208 54 887.3 0 54 209 66 887.3 0 66 210 84 887.3 0 84 211 77 887.3 0 77 212 78 887.3 0 78 213 61 887.3 0 61 214 101 887.3 0 101 215 88 887.3 0 88 216 78 887.3 0 78 217 86 887.3 0 86 218 67 887.3 0 67 219 67 887.3 0 67 220 70 887.3 0 70 221 65 887.3 0 65 222 57 887.3 0 57 223 71 887.3 0 71 224 77 887.3 0 77 225 103 887.3 0 103 226 88 887.3 0 88 227 72 887.3 0 72 228 77 887.3 0 77 229 82 887.3 0 82 230 71 887.3 0 71 231 72 887.3 0 72 232 69 887.3 0 69 233 75 887.3 0 75 234 85 887.3 0 85 235 73 887.3 0 73 236 66 887.3 0 66 237 52 887.3 0 52 238 180 887.3 0 180 239 244 887.3 0 244 240 135 887.3 0 135 241 67 887.3 0 67 242 93 887.3 0 93 243 73 887.3 0 73 244 65 887.3 0 65 245 72 887.3 0 72 246 64 887.3 0 64 247 93 887.3 0 93 248 101 887.3 0 101 249 64 887.3 0 64 250 66 887.3 0 66 251 88 887.3 0 88 252 78 887.3 0 78 253 57 887.3 0 57 254 71 887.3 0 71 255 88 887.3 0 88 256 69 887.3 0 69 257 106 887.3 0 106 258 116 887.3 0 116 259 137 887.3 0 137 260 174 887.3 0 174 261 96 887.3 0 96 262 87 887.3 0 87 263 136 887.3 0 136 264 94 887.3 0 94 265 108 887.3 0 108 266 132 887.3 0 132 267 118 887.3 0 118 268 135 887.3 0 135 269 130 887.3 0 130 270 182 887.3 0 182 271 195 887.3 0 195 272 433 887.3 0 433 273 6153 887.3 0 6153 274 6133 887.3 0 6133 275 3450 887.3 0 3450 276 210 887.3 0 210 277 260 887.3 0 260 278 185 887.3 0 185 279 173 887.3 0 173 280 171 887.3 0 171 281 93 887.3 0 93 282 123 887.3 0 123 283 255 887.3 0 255 284 337 887.3 0 337 285 391 887.3 0 391 286 910 887.3 0 910 287 911 887.3 0 911 288 1821 887.3 0 1821 289 176 887.3 0 176 290 136 887.3 0 136 291 222 887.3 0 222 292 396 887.3 0 396 293 3657 887.3 0 3657 294 2865 887.3 0 2865 295 1470 887.3 0 1470 296 213 887.3 0 213 297 723 887.3 0 723 298 2896 887.3 0 2896 299 8842 887.3 0 8842 300 8532 887.3 0 8532 301 5994 887.3 0 5994 Finished in 5.10 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 69,425 Reads with adapters: 24,961 (36.0%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 24,945 (35.9%) Total basepairs processed: 18,943,005 bp Total written (filtered): 6,881,152 bp (36.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 24961 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 153 0.0 3 0 149 4 17 24667 0.0 3 24157 492 17 1 18 64 0.0 3 3 59 2 19 17 0.0 3 4 0 12 1 20 10 0.0 3 1 0 0 9 35 6 0.0 3 4 2 37 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 55 10 0.0 3 0 0 0 10 58 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 215 3 0.0 3 0 0 0 3 221 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.42 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 24,945 Reads with adapters: 46 (0.2%) Reads written (passing filters): 24,945 (100.0%) Total basepairs processed: 6,881,152 bp Total written (filtered): 6,881,006 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 46 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.1% C: 4.3% G: 6.5% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 41 389.8 0 41 4 2 97.4 0 2 5 3 24.4 0 3