This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 176.08 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 188,220 (5.2%) Reads that were too short: 16,611 (0.5%) Reads written (passing filters): 171,609 (4.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 47,054,252 bp (4.3%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 188220 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4717 887.3 0 4717 7 60749 887.3 0 60749 8 73740 887.3 0 73740 9 110 887.3 0 110 10 57 887.3 0 57 11 62 887.3 0 62 12 36 887.3 0 36 13 42 887.3 0 42 14 71 887.3 0 71 15 60 887.3 0 60 16 31 887.3 0 31 17 21 887.3 0 21 18 46 887.3 0 46 19 40 887.3 0 40 20 43 887.3 0 43 21 50 887.3 0 50 22 39 887.3 0 39 23 33 887.3 0 33 24 48 887.3 0 48 25 44 887.3 0 44 26 49 887.3 0 49 27 47 887.3 0 47 28 45 887.3 0 45 29 38 887.3 0 38 30 145 887.3 0 145 31 108 887.3 0 108 32 58 887.3 0 58 33 55 887.3 0 55 34 78 887.3 0 78 35 53 887.3 0 53 36 44 887.3 0 44 37 62 887.3 0 62 38 46 887.3 0 46 39 48 887.3 0 48 40 70 887.3 0 70 41 66 887.3 0 66 42 54 887.3 0 54 43 73 887.3 0 73 44 65 887.3 0 65 45 65 887.3 0 65 46 71 887.3 0 71 47 112 887.3 0 112 48 96 887.3 0 96 49 132 887.3 0 132 50 2774 887.3 0 2774 51 3082 887.3 0 3082 52 3510 887.3 0 3510 53 63 887.3 0 63 54 93 887.3 0 93 55 38 887.3 0 38 56 35 887.3 0 35 57 48 887.3 0 48 58 57 887.3 0 57 59 43 887.3 0 43 60 58 887.3 0 58 61 41 887.3 0 41 62 35 887.3 0 35 63 51 887.3 0 51 64 55 887.3 0 55 65 210 887.3 0 210 66 68 887.3 0 68 67 46 887.3 0 46 68 50 887.3 0 50 69 49 887.3 0 49 70 33 887.3 0 33 71 50 887.3 0 50 72 62 887.3 0 62 73 73 887.3 0 73 74 233 887.3 0 233 75 324 887.3 0 324 76 489 887.3 0 489 77 45 887.3 0 45 78 94 887.3 0 94 79 66 887.3 0 66 80 64 887.3 0 64 81 54 887.3 0 54 82 123 887.3 0 123 83 46 887.3 0 46 84 48 887.3 0 48 85 47 887.3 0 47 86 42 887.3 0 42 87 43 887.3 0 43 88 45 887.3 0 45 89 46 887.3 0 46 90 38 887.3 0 38 91 51 887.3 0 51 92 39 887.3 0 39 93 50 887.3 0 50 94 36 887.3 0 36 95 34 887.3 0 34 96 47 887.3 0 47 97 53 887.3 0 53 98 75 887.3 0 75 99 49 887.3 0 49 100 60 887.3 0 60 101 63 887.3 0 63 102 48 887.3 0 48 103 49 887.3 0 49 104 46 887.3 0 46 105 46 887.3 0 46 106 56 887.3 0 56 107 55 887.3 0 55 108 45 887.3 0 45 109 43 887.3 0 43 110 35 887.3 0 35 111 59 887.3 0 59 112 68 887.3 0 68 113 48 887.3 0 48 114 118 887.3 0 118 115 95 887.3 0 95 116 123 887.3 0 123 117 75 887.3 0 75 118 86 887.3 0 86 119 81 887.3 0 81 120 72 887.3 0 72 121 58 887.3 0 58 122 86 887.3 0 86 123 71 887.3 0 71 124 119 887.3 0 119 125 915 887.3 0 915 126 833 887.3 0 833 127 698 887.3 0 698 128 54 887.3 0 54 129 88 887.3 0 88 130 160 887.3 0 160 131 185 887.3 0 185 132 166 887.3 0 166 133 81 887.3 0 81 134 66 887.3 0 66 135 182 887.3 0 182 136 535 887.3 0 535 137 139 887.3 0 139 138 104 887.3 0 104 139 49 887.3 0 49 140 54 887.3 0 54 141 50 887.3 0 50 142 48 887.3 0 48 143 52 887.3 0 52 144 58 887.3 0 58 145 65 887.3 0 65 146 46 887.3 0 46 147 56 887.3 0 56 148 40 887.3 0 40 149 47 887.3 0 47 150 46 887.3 0 46 151 58 887.3 0 58 152 29 887.3 0 29 153 59 887.3 0 59 154 57 887.3 0 57 155 42 887.3 0 42 156 59 887.3 0 59 157 53 887.3 0 53 158 55 887.3 0 55 159 54 887.3 0 54 160 73 887.3 0 73 161 67 887.3 0 67 162 60 887.3 0 60 163 84 887.3 0 84 164 1407 887.3 0 1407 165 673 887.3 0 673 166 738 887.3 0 738 167 67 887.3 0 67 168 93 887.3 0 93 169 97 887.3 0 97 170 37 887.3 0 37 171 51 887.3 0 51 172 39 887.3 0 39 173 51 887.3 0 51 174 54 887.3 0 54 175 45 887.3 0 45 176 56 887.3 0 56 177 59 887.3 0 59 178 59 887.3 0 59 179 84 887.3 0 84 180 51 887.3 0 51 181 68 887.3 0 68 182 62 887.3 0 62 183 84 887.3 0 84 184 48 887.3 0 48 185 61 887.3 0 61 186 93 887.3 0 93 187 63 887.3 0 63 188 66 887.3 0 66 189 89 887.3 0 89 190 93 887.3 0 93 191 266 887.3 0 266 192 204 887.3 0 204 193 217 887.3 0 217 194 144 887.3 0 144 195 177 887.3 0 177 196 1438 887.3 0 1438 197 1390 887.3 0 1390 198 1075 887.3 0 1075 199 196 887.3 0 196 200 110 887.3 0 110 201 108 887.3 0 108 202 87 887.3 0 87 203 72 887.3 0 72 204 336 887.3 0 336 205 262 887.3 0 262 206 174 887.3 0 174 207 97 887.3 0 97 208 119 887.3 0 119 209 95 887.3 0 95 210 108 887.3 0 108 211 127 887.3 0 127 212 120 887.3 0 120 213 123 887.3 0 123 214 75 887.3 0 75 215 102 887.3 0 102 216 863 887.3 0 863 217 926 887.3 0 926 218 642 887.3 0 642 219 277 887.3 0 277 220 89 887.3 0 89 221 102 887.3 0 102 222 77 887.3 0 77 223 102 887.3 0 102 224 97 887.3 0 97 225 96 887.3 0 96 226 69 887.3 0 69 227 86 887.3 0 86 228 78 887.3 0 78 229 70 887.3 0 70 230 93 887.3 0 93 231 71 887.3 0 71 232 58 887.3 0 58 233 56 887.3 0 56 234 74 887.3 0 74 235 73 887.3 0 73 236 45 887.3 0 45 237 68 887.3 0 68 238 138 887.3 0 138 239 121 887.3 0 121 240 150 887.3 0 150 241 132 887.3 0 132 242 90 887.3 0 90 243 87 887.3 0 87 244 57 887.3 0 57 245 93 887.3 0 93 246 84 887.3 0 84 247 525 887.3 0 525 248 859 887.3 0 859 249 1625 887.3 0 1625 250 907 887.3 0 907 251 137 887.3 0 137 252 132 887.3 0 132 253 72 887.3 0 72 254 83 887.3 0 83 255 121 887.3 0 121 256 84 887.3 0 84 257 81 887.3 0 81 258 82 887.3 0 82 259 73 887.3 0 73 260 75 887.3 0 75 261 64 887.3 0 64 262 97 887.3 0 97 263 86 887.3 0 86 264 52 887.3 0 52 265 68 887.3 0 68 266 58 887.3 0 58 267 63 887.3 0 63 268 71 887.3 0 71 269 185 887.3 0 185 270 301 887.3 0 301 271 110 887.3 0 110 272 80 887.3 0 80 273 65 887.3 0 65 274 69 887.3 0 69 275 61 887.3 0 61 276 89 887.3 0 89 277 71 887.3 0 71 278 86 887.3 0 86 279 71 887.3 0 71 280 57 887.3 0 57 281 43 887.3 0 43 282 80 887.3 0 80 283 69 887.3 0 69 284 101 887.3 0 101 285 66 887.3 0 66 286 78 887.3 0 78 287 77 887.3 0 77 288 47 887.3 0 47 289 194 887.3 0 194 290 300 887.3 0 300 291 358 887.3 0 358 292 167 887.3 0 167 293 128 887.3 0 128 294 72 887.3 0 72 295 70 887.3 0 70 296 73 887.3 0 73 297 237 887.3 0 237 298 220 887.3 0 220 299 267 887.3 0 267 300 207 887.3 0 207 301 136 887.3 0 136 Finished in 12.53 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 171,609 Reads with adapters: 63,648 (37.1%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 63,597 (37.1%) Total basepairs processed: 47,054,252 bp Total written (filtered): 17,605,829 bp (37.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 63648 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 12 0.0 3 0 0 10 2 16 320 0.0 3 0 313 7 17 62995 0.0 3 61695 1249 47 4 18 95 0.0 3 4 91 19 23 0.0 3 3 1 15 4 20 56 0.0 3 0 0 0 56 22 1 0.0 3 0 0 1 26 1 0.0 3 0 0 0 1 27 18 0.0 3 0 0 0 18 30 3 0.0 3 0 0 0 3 34 1 0.0 3 1 38 1 0.0 3 0 0 0 1 46 16 0.0 3 0 0 0 16 49 5 0.0 3 0 0 0 5 53 3 0.0 3 0 0 0 3 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 1 1 78 1 0.0 3 0 0 0 1 87 5 0.0 3 0 0 0 5 88 1 0.0 3 0 0 0 1 90 7 0.0 3 0 0 0 7 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 97 7 0.0 3 0 0 0 7 100 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 117 3 0.0 3 0 0 0 3 118 8 0.0 3 0 0 0 8 121 5 0.0 3 0 0 0 5 135 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 169 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 1 205 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 248 2 0.0 3 0 0 0 2 250 4 0.0 3 0 0 0 4 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.70 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 63,597 Reads with adapters: 180 (0.3%) Reads written (passing filters): 63,597 (100.0%) Total basepairs processed: 17,605,829 bp Total written (filtered): 17,605,279 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 180 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.4% C: 39.4% G: 16.7% T: 24.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 173 993.7 0 173 4 4 248.4 0 4 5 3 62.1 0 3