This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 178.97 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 491,827 (13.5%) Reads that were too short: 120,007 (3.3%) Reads written (passing filters): 371,820 (10.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 101,622,238 bp (9.3%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 491827 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3611 887.3 0 3611 7 166582 887.3 0 166582 8 2035 887.3 0 2035 9 141827 887.3 0 141827 10 141 887.3 0 141 11 47 887.3 0 47 12 47 887.3 0 47 13 46 887.3 0 46 14 58 887.3 0 58 15 55 887.3 0 55 16 74 887.3 0 74 17 74 887.3 0 74 18 63 887.3 0 63 19 45 887.3 0 45 20 37 887.3 0 37 21 42 887.3 0 42 22 43 887.3 0 43 23 47 887.3 0 47 24 21 887.3 0 21 25 44 887.3 0 44 26 39 887.3 0 39 27 476 887.3 0 476 28 72 887.3 0 72 29 37 887.3 0 37 30 37 887.3 0 37 31 42 887.3 0 42 32 25 887.3 0 25 33 45 887.3 0 45 34 43 887.3 0 43 35 37 887.3 0 37 36 25 887.3 0 25 37 31 887.3 0 31 38 42 887.3 0 42 39 44 887.3 0 44 40 66 887.3 0 66 41 82 887.3 0 82 42 37 887.3 0 37 43 36 887.3 0 36 44 55 887.3 0 55 45 42 887.3 0 42 46 52 887.3 0 52 47 38 887.3 0 38 48 45 887.3 0 45 49 58 887.3 0 58 50 63 887.3 0 63 51 55 887.3 0 55 52 49 887.3 0 49 53 67 887.3 0 67 54 62 887.3 0 62 55 67 887.3 0 67 56 122 887.3 0 122 57 1158 887.3 0 1158 58 965 887.3 0 965 59 2148 887.3 0 2148 60 50 887.3 0 50 61 29 887.3 0 29 62 48 887.3 0 48 63 43 887.3 0 43 64 49 887.3 0 49 65 44 887.3 0 44 66 39 887.3 0 39 67 41 887.3 0 41 68 44 887.3 0 44 69 57 887.3 0 57 70 27 887.3 0 27 71 56 887.3 0 56 72 52 887.3 0 52 73 41 887.3 0 41 74 41 887.3 0 41 75 34 887.3 0 34 76 35 887.3 0 35 77 44 887.3 0 44 78 53 887.3 0 53 79 65 887.3 0 65 80 83 887.3 0 83 81 60 887.3 0 60 82 66 887.3 0 66 83 79 887.3 0 79 84 73 887.3 0 73 85 65 887.3 0 65 86 93 887.3 0 93 87 84 887.3 0 84 88 221 887.3 0 221 89 147 887.3 0 147 90 236 887.3 0 236 91 174 887.3 0 174 92 105 887.3 0 105 93 53 887.3 0 53 94 68 887.3 0 68 95 71 887.3 0 71 96 70 887.3 0 70 97 59 887.3 0 59 98 76 887.3 0 76 99 79 887.3 0 79 100 63 887.3 0 63 101 95 887.3 0 95 102 90 887.3 0 90 103 91 887.3 0 91 104 91 887.3 0 91 105 171 887.3 0 171 106 140 887.3 0 140 107 167 887.3 0 167 108 149 887.3 0 149 109 99 887.3 0 99 110 81 887.3 0 81 111 50 887.3 0 50 112 67 887.3 0 67 113 66 887.3 0 66 114 84 887.3 0 84 115 81 887.3 0 81 116 89 887.3 0 89 117 114 887.3 0 114 118 62 887.3 0 62 119 71 887.3 0 71 120 99 887.3 0 99 121 107 887.3 0 107 122 467 887.3 0 467 123 584 887.3 0 584 124 696 887.3 0 696 125 70 887.3 0 70 126 68 887.3 0 68 127 84 887.3 0 84 128 72 887.3 0 72 129 74 887.3 0 74 130 114 887.3 0 114 131 99 887.3 0 99 132 101 887.3 0 101 133 127 887.3 0 127 134 152 887.3 0 152 135 293 887.3 0 293 136 107 887.3 0 107 137 95 887.3 0 95 138 91 887.3 0 91 139 116 887.3 0 116 140 110 887.3 0 110 141 229 887.3 0 229 142 315 887.3 0 315 143 360 887.3 0 360 144 154 887.3 0 154 145 163 887.3 0 163 146 235 887.3 0 235 147 362 887.3 0 362 148 595 887.3 0 595 149 582 887.3 0 582 150 579 887.3 0 579 151 8035 887.3 0 8035 152 13241 887.3 0 13241 153 9933 887.3 0 9933 154 145 887.3 0 145 155 152 887.3 0 152 156 144 887.3 0 144 157 462 887.3 0 462 158 514 887.3 0 514 159 491 887.3 0 491 160 100 887.3 0 100 161 156 887.3 0 156 162 231 887.3 0 231 163 253 887.3 0 253 164 153 887.3 0 153 165 69 887.3 0 69 166 93 887.3 0 93 167 87 887.3 0 87 168 103 887.3 0 103 169 82 887.3 0 82 170 106 887.3 0 106 171 160 887.3 0 160 172 125 887.3 0 125 173 70 887.3 0 70 174 73 887.3 0 73 175 90 887.3 0 90 176 163 887.3 0 163 177 119 887.3 0 119 178 101 887.3 0 101 179 68 887.3 0 68 180 79 887.3 0 79 181 84 887.3 0 84 182 191 887.3 0 191 183 194 887.3 0 194 184 194 887.3 0 194 185 82 887.3 0 82 186 203 887.3 0 203 187 175 887.3 0 175 188 141 887.3 0 141 189 173 887.3 0 173 190 78 887.3 0 78 191 75 887.3 0 75 192 88 887.3 0 88 193 97 887.3 0 97 194 95 887.3 0 95 195 155 887.3 0 155 196 227 887.3 0 227 197 119 887.3 0 119 198 119 887.3 0 119 199 68 887.3 0 68 200 122 887.3 0 122 201 266 887.3 0 266 202 144 887.3 0 144 203 95 887.3 0 95 204 101 887.3 0 101 205 114 887.3 0 114 206 188 887.3 0 188 207 212 887.3 0 212 208 373 887.3 0 373 209 635 887.3 0 635 210 1071 887.3 0 1071 211 1128 887.3 0 1128 212 1297 887.3 0 1297 213 16641 887.3 0 16641 214 19239 887.3 0 19239 215 60046 887.3 0 60046 216 1190 887.3 0 1190 217 125 887.3 0 125 218 100 887.3 0 100 219 109 887.3 0 109 220 226 887.3 0 226 221 902 887.3 0 902 222 1579 887.3 0 1579 223 2336 887.3 0 2336 224 477 887.3 0 477 225 94 887.3 0 94 226 81 887.3 0 81 227 102 887.3 0 102 228 74 887.3 0 74 229 88 887.3 0 88 230 68 887.3 0 68 231 149 887.3 0 149 232 138 887.3 0 138 233 102 887.3 0 102 234 96 887.3 0 96 235 119 887.3 0 119 236 126 887.3 0 126 237 87 887.3 0 87 238 108 887.3 0 108 239 131 887.3 0 131 240 107 887.3 0 107 241 112 887.3 0 112 242 112 887.3 0 112 243 118 887.3 0 118 244 162 887.3 0 162 245 180 887.3 0 180 246 140 887.3 0 140 247 73 887.3 0 73 248 85 887.3 0 85 249 72 887.3 0 72 250 86 887.3 0 86 251 108 887.3 0 108 252 94 887.3 0 94 253 96 887.3 0 96 254 109 887.3 0 109 255 133 887.3 0 133 256 111 887.3 0 111 257 275 887.3 0 275 258 413 887.3 0 413 259 418 887.3 0 418 260 227 887.3 0 227 261 127 887.3 0 127 262 141 887.3 0 141 263 250 887.3 0 250 264 341 887.3 0 341 265 263 887.3 0 263 266 114 887.3 0 114 267 85 887.3 0 85 268 102 887.3 0 102 269 188 887.3 0 188 270 216 887.3 0 216 271 162 887.3 0 162 272 169 887.3 0 169 273 301 887.3 0 301 274 347 887.3 0 347 275 150 887.3 0 150 276 139 887.3 0 139 277 167 887.3 0 167 278 126 887.3 0 126 279 104 887.3 0 104 280 105 887.3 0 105 281 300 887.3 0 300 282 364 887.3 0 364 283 233 887.3 0 233 284 138 887.3 0 138 285 161 887.3 0 161 286 144 887.3 0 144 287 107 887.3 0 107 288 95 887.3 0 95 289 135 887.3 0 135 290 169 887.3 0 169 291 124 887.3 0 124 292 114 887.3 0 114 293 92 887.3 0 92 294 131 887.3 0 131 295 216 887.3 0 216 296 180 887.3 0 180 297 152 887.3 0 152 298 119 887.3 0 119 299 108 887.3 0 108 300 129 887.3 0 129 301 77 887.3 0 77 Finished in 26.93 s (72 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 371,820 Reads with adapters: 145,081 (39.0%) Reads that were too short: 65 (0.0%) Reads written (passing filters): 145,016 (39.0%) Total basepairs processed: 101,622,238 bp Total written (filtered): 39,871,045 bp (39.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 145081 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 24 0.0 3 0 0 23 1 16 897 0.0 3 0 865 31 1 17 143295 0.0 3 140525 2694 68 8 18 336 0.0 3 25 301 10 19 102 0.0 3 4 1 86 11 20 274 0.0 3 184 3 0 87 22 2 0.0 3 1 0 0 1 23 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 29 2 0.0 3 2 30 1 0.0 3 1 32 2 0.0 3 1 0 0 1 41 1 0.0 3 1 42 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 160 66 0.0 3 0 0 0 66 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 210 1 0.0 3 1 216 2 0.0 3 0 0 0 2 217 31 0.0 3 0 0 0 31 218 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 249 1 0.0 3 1 260 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 295 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.50 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 145,016 Reads with adapters: 1,481 (1.0%) Reads written (passing filters): 145,016 (100.0%) Total basepairs processed: 39,871,045 bp Total written (filtered): 39,866,517 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1481 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.2% C: 5.7% G: 29.6% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1422 2265.9 0 1422 4 51 566.5 0 51 5 3 141.6 0 3 6 1 35.4 0 1 7 1 8.9 0 1 9 1 0.6 0 0 1 10 1 0.1 1 0 1 11 1 0.0 1 0 1