This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 176.26 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 269,685 (7.4%) Reads that were too short: 16,031 (0.4%) Reads written (passing filters): 253,654 (7.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 57,772,802 bp (5.3%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 269685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3143 887.3 0 3143 7 79503 887.3 0 79503 8 57043 887.3 0 57043 9 159 887.3 0 159 10 172 887.3 0 172 11 71 887.3 0 71 12 109 887.3 0 109 13 81 887.3 0 81 14 87 887.3 0 87 15 88 887.3 0 88 16 64 887.3 0 64 17 115 887.3 0 115 18 110 887.3 0 110 19 172 887.3 0 172 20 247 887.3 0 247 21 200 887.3 0 200 22 89 887.3 0 89 23 115 887.3 0 115 24 63 887.3 0 63 25 70 887.3 0 70 26 120 887.3 0 120 27 77 887.3 0 77 28 72 887.3 0 72 29 99 887.3 0 99 30 103 887.3 0 103 31 100 887.3 0 100 32 86 887.3 0 86 33 98 887.3 0 98 34 121 887.3 0 121 35 101 887.3 0 101 36 93 887.3 0 93 37 97 887.3 0 97 38 167 887.3 0 167 39 188 887.3 0 188 40 140 887.3 0 140 41 223 887.3 0 223 42 89 887.3 0 89 43 89 887.3 0 89 44 101 887.3 0 101 45 109 887.3 0 109 46 113 887.3 0 113 47 106 887.3 0 106 48 100 887.3 0 100 49 98 887.3 0 98 50 98 887.3 0 98 51 111 887.3 0 111 52 91 887.3 0 91 53 79 887.3 0 79 54 89 887.3 0 89 55 72 887.3 0 72 56 83 887.3 0 83 57 115 887.3 0 115 58 104 887.3 0 104 59 83 887.3 0 83 60 87 887.3 0 87 61 87 887.3 0 87 62 87 887.3 0 87 63 65 887.3 0 65 64 75 887.3 0 75 65 98 887.3 0 98 66 75 887.3 0 75 67 81 887.3 0 81 68 107 887.3 0 107 69 90 887.3 0 90 70 82 887.3 0 82 71 95 887.3 0 95 72 95 887.3 0 95 73 139 887.3 0 139 74 95 887.3 0 95 75 121 887.3 0 121 76 219 887.3 0 219 77 278 887.3 0 278 78 166 887.3 0 166 79 96 887.3 0 96 80 111 887.3 0 111 81 87 887.3 0 87 82 104 887.3 0 104 83 123 887.3 0 123 84 93 887.3 0 93 85 91 887.3 0 91 86 144 887.3 0 144 87 124 887.3 0 124 88 107 887.3 0 107 89 96 887.3 0 96 90 134 887.3 0 134 91 156 887.3 0 156 92 195 887.3 0 195 93 723 887.3 0 723 94 781 887.3 0 781 95 589 887.3 0 589 96 744 887.3 0 744 97 906 887.3 0 906 98 434 887.3 0 434 99 87 887.3 0 87 100 95 887.3 0 95 101 70 887.3 0 70 102 94 887.3 0 94 103 72 887.3 0 72 104 89 887.3 0 89 105 158 887.3 0 158 106 70 887.3 0 70 107 135 887.3 0 135 108 120 887.3 0 120 109 101 887.3 0 101 110 118 887.3 0 118 111 103 887.3 0 103 112 96 887.3 0 96 113 90 887.3 0 90 114 68 887.3 0 68 115 94 887.3 0 94 116 103 887.3 0 103 117 89 887.3 0 89 118 76 887.3 0 76 119 67 887.3 0 67 120 58 887.3 0 58 121 71 887.3 0 71 122 85 887.3 0 85 123 84 887.3 0 84 124 60 887.3 0 60 125 86 887.3 0 86 126 79 887.3 0 79 127 93 887.3 0 93 128 94 887.3 0 94 129 193 887.3 0 193 130 668 887.3 0 668 131 524 887.3 0 524 132 437 887.3 0 437 133 138 887.3 0 138 134 156 887.3 0 156 135 149 887.3 0 149 136 119 887.3 0 119 137 180 887.3 0 180 138 100 887.3 0 100 139 92 887.3 0 92 140 157 887.3 0 157 141 101 887.3 0 101 142 353 887.3 0 353 143 960 887.3 0 960 144 2215 887.3 0 2215 145 242 887.3 0 242 146 65 887.3 0 65 147 97 887.3 0 97 148 56 887.3 0 56 149 88 887.3 0 88 150 91 887.3 0 91 151 136 887.3 0 136 152 339 887.3 0 339 153 82 887.3 0 82 154 127 887.3 0 127 155 105 887.3 0 105 156 203 887.3 0 203 157 131 887.3 0 131 158 134 887.3 0 134 159 108 887.3 0 108 160 106 887.3 0 106 161 127 887.3 0 127 162 201 887.3 0 201 163 244 887.3 0 244 164 339 887.3 0 339 165 364 887.3 0 364 166 461 887.3 0 461 167 1093 887.3 0 1093 168 2378 887.3 0 2378 169 44542 887.3 0 44542 170 17602 887.3 0 17602 171 18455 887.3 0 18455 172 309 887.3 0 309 173 93 887.3 0 93 174 231 887.3 0 231 175 113 887.3 0 113 176 297 887.3 0 297 177 67 887.3 0 67 178 106 887.3 0 106 179 83 887.3 0 83 180 104 887.3 0 104 181 83 887.3 0 83 182 66 887.3 0 66 183 70 887.3 0 70 184 105 887.3 0 105 185 93 887.3 0 93 186 77 887.3 0 77 187 72 887.3 0 72 188 65 887.3 0 65 189 67 887.3 0 67 190 81 887.3 0 81 191 71 887.3 0 71 192 131 887.3 0 131 193 172 887.3 0 172 194 90 887.3 0 90 195 56 887.3 0 56 196 87 887.3 0 87 197 65 887.3 0 65 198 114 887.3 0 114 199 58 887.3 0 58 200 85 887.3 0 85 201 84 887.3 0 84 202 106 887.3 0 106 203 100 887.3 0 100 204 129 887.3 0 129 205 90 887.3 0 90 206 114 887.3 0 114 207 70 887.3 0 70 208 94 887.3 0 94 209 97 887.3 0 97 210 107 887.3 0 107 211 142 887.3 0 142 212 109 887.3 0 109 213 108 887.3 0 108 214 100 887.3 0 100 215 131 887.3 0 131 216 166 887.3 0 166 217 140 887.3 0 140 218 87 887.3 0 87 219 91 887.3 0 91 220 84 887.3 0 84 221 207 887.3 0 207 222 254 887.3 0 254 223 155 887.3 0 155 224 675 887.3 0 675 225 77 887.3 0 77 226 79 887.3 0 79 227 73 887.3 0 73 228 102 887.3 0 102 229 79 887.3 0 79 230 132 887.3 0 132 231 182 887.3 0 182 232 165 887.3 0 165 233 135 887.3 0 135 234 126 887.3 0 126 235 180 887.3 0 180 236 362 887.3 0 362 237 457 887.3 0 457 238 375 887.3 0 375 239 83 887.3 0 83 240 118 887.3 0 118 241 113 887.3 0 113 242 143 887.3 0 143 243 84 887.3 0 84 244 77 887.3 0 77 245 63 887.3 0 63 246 100 887.3 0 100 247 78 887.3 0 78 248 70 887.3 0 70 249 78 887.3 0 78 250 90 887.3 0 90 251 74 887.3 0 74 252 81 887.3 0 81 253 166 887.3 0 166 254 134 887.3 0 134 255 130 887.3 0 130 256 98 887.3 0 98 257 80 887.3 0 80 258 78 887.3 0 78 259 69 887.3 0 69 260 89 887.3 0 89 261 150 887.3 0 150 262 208 887.3 0 208 263 272 887.3 0 272 264 134 887.3 0 134 265 81 887.3 0 81 266 91 887.3 0 91 267 95 887.3 0 95 268 82 887.3 0 82 269 87 887.3 0 87 270 131 887.3 0 131 271 490 887.3 0 490 272 750 887.3 0 750 273 621 887.3 0 621 274 216 887.3 0 216 275 80 887.3 0 80 276 90 887.3 0 90 277 103 887.3 0 103 278 104 887.3 0 104 279 221 887.3 0 221 280 194 887.3 0 194 281 116 887.3 0 116 282 115 887.3 0 115 283 135 887.3 0 135 284 131 887.3 0 131 285 128 887.3 0 128 286 115 887.3 0 115 287 100 887.3 0 100 288 111 887.3 0 111 289 104 887.3 0 104 290 172 887.3 0 172 291 107 887.3 0 107 292 289 887.3 0 289 293 296 887.3 0 296 294 195 887.3 0 195 295 116 887.3 0 116 296 466 887.3 0 466 297 453 887.3 0 453 298 329 887.3 0 329 299 175 887.3 0 175 300 134 887.3 0 134 301 68 887.3 0 68 Finished in 17.85 s (70 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 253,654 Reads with adapters: 62,787 (24.8%) Reads that were too short: 51 (0.0%) Reads written (passing filters): 62,736 (24.7%) Total basepairs processed: 57,772,802 bp Total written (filtered): 17,062,115 bp (29.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 62787 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 28 0.0 3 0 0 21 6 1 19 715 0.0 3 0 603 58 36 18 20 60242 0.0 4 57173 2543 399 98 29 21 158 0.0 4 27 117 9 4 1 22 29 0.0 4 3 9 15 0 2 23 896 0.0 4 719 26 6 118 27 24 7 0.0 4 2 1 0 1 3 27 2 0.0 4 1 0 0 0 1 28 2 0.0 4 0 0 1 0 1 41 178 0.0 4 0 0 0 1 177 42 2 0.0 4 0 0 0 0 2 44 3 0.0 4 0 0 0 0 3 52 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 1 74 1 0.0 4 1 75 3 0.0 4 3 76 4 0.0 4 4 77 6 0.0 4 5 0 0 1 82 4 0.0 4 0 0 0 0 4 86 1 0.0 4 1 87 2 0.0 4 2 88 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 433 0.0 4 0 0 0 0 433 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 4 0.0 4 0 0 0 0 4 154 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 184 6 0.0 4 0 0 0 0 6 196 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 3 0.0 4 0 0 0 0 3 226 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 239 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.94 s (63 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 62,736 Reads with adapters: 12,971 (20.7%) Reads written (passing filters): 62,736 (100.0%) Total basepairs processed: 17,062,115 bp Total written (filtered): 16,673,006 bp (97.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 12971 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.6% C: 8.8% G: 84.3% T: 3.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 97 980.2 0 97 4 29 245.1 0 29 5 2 61.3 0 2 9 95 0.2 0 2 93 10 1553 0.1 1 66 1487 11 10134 0.0 1 189 9945 12 67 0.0 1 2 65 13 8 0.0 1 0 8 14 8 0.0 1 0 8 15 2 0.0 1 0 2 16 1 0.0 1 0 1 86 1 0.0 1 1 110 1 0.0 1 1 148 2 0.0 1 2 151 7 0.0 1 3 4 152 1 0.0 1 1 190 2 0.0 1 0 2 209 2 0.0 1 1 1 223 1 0.0 1 1 229 1 0.0 1 1 258 1 0.0 1 1 259 164 0.0 1 153 11 260 16 0.0 1 15 1 261 5 0.0 1 5 265 2 0.0 1 2 269 2 0.0 1 2 270 354 0.0 1 323 31 271 378 0.0 1 348 30 272 25 0.0 1 23 2 273 9 0.0 1 8 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.