This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 177.34 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 286,798 (7.9%) Reads that were too short: 14,126 (0.4%) Reads written (passing filters): 272,672 (7.5%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 60,928,221 bp (5.6%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 286798 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2760 887.3 0 2760 7 68230 887.3 0 68230 8 74159 887.3 0 74159 9 148 887.3 0 148 10 107 887.3 0 107 11 80 887.3 0 80 12 107 887.3 0 107 13 79 887.3 0 79 14 95 887.3 0 95 15 88 887.3 0 88 16 78 887.3 0 78 17 74 887.3 0 74 18 96 887.3 0 96 19 147 887.3 0 147 20 173 887.3 0 173 21 179 887.3 0 179 22 95 887.3 0 95 23 110 887.3 0 110 24 76 887.3 0 76 25 87 887.3 0 87 26 124 887.3 0 124 27 91 887.3 0 91 28 71 887.3 0 71 29 104 887.3 0 104 30 111 887.3 0 111 31 96 887.3 0 96 32 83 887.3 0 83 33 82 887.3 0 82 34 95 887.3 0 95 35 99 887.3 0 99 36 114 887.3 0 114 37 101 887.3 0 101 38 146 887.3 0 146 39 125 887.3 0 125 40 119 887.3 0 119 41 84 887.3 0 84 42 101 887.3 0 101 43 87 887.3 0 87 44 104 887.3 0 104 45 119 887.3 0 119 46 99 887.3 0 99 47 108 887.3 0 108 48 103 887.3 0 103 49 102 887.3 0 102 50 84 887.3 0 84 51 89 887.3 0 89 52 92 887.3 0 92 53 97 887.3 0 97 54 84 887.3 0 84 55 86 887.3 0 86 56 90 887.3 0 90 57 134 887.3 0 134 58 123 887.3 0 123 59 106 887.3 0 106 60 106 887.3 0 106 61 119 887.3 0 119 62 102 887.3 0 102 63 72 887.3 0 72 64 97 887.3 0 97 65 95 887.3 0 95 66 104 887.3 0 104 67 100 887.3 0 100 68 80 887.3 0 80 69 115 887.3 0 115 70 95 887.3 0 95 71 113 887.3 0 113 72 88 887.3 0 88 73 103 887.3 0 103 74 90 887.3 0 90 75 108 887.3 0 108 76 190 887.3 0 190 77 257 887.3 0 257 78 171 887.3 0 171 79 110 887.3 0 110 80 127 887.3 0 127 81 92 887.3 0 92 82 108 887.3 0 108 83 89 887.3 0 89 84 107 887.3 0 107 85 83 887.3 0 83 86 84 887.3 0 84 87 126 887.3 0 126 88 99 887.3 0 99 89 86 887.3 0 86 90 83 887.3 0 83 91 121 887.3 0 121 92 114 887.3 0 114 93 401 887.3 0 401 94 456 887.3 0 456 95 296 887.3 0 296 96 358 887.3 0 358 97 338 887.3 0 338 98 204 887.3 0 204 99 90 887.3 0 90 100 101 887.3 0 101 101 93 887.3 0 93 102 67 887.3 0 67 103 80 887.3 0 80 104 77 887.3 0 77 105 160 887.3 0 160 106 86 887.3 0 86 107 160 887.3 0 160 108 78 887.3 0 78 109 76 887.3 0 76 110 88 887.3 0 88 111 65 887.3 0 65 112 91 887.3 0 91 113 95 887.3 0 95 114 80 887.3 0 80 115 101 887.3 0 101 116 94 887.3 0 94 117 109 887.3 0 109 118 73 887.3 0 73 119 65 887.3 0 65 120 59 887.3 0 59 121 51 887.3 0 51 122 93 887.3 0 93 123 101 887.3 0 101 124 74 887.3 0 74 125 110 887.3 0 110 126 83 887.3 0 83 127 98 887.3 0 98 128 92 887.3 0 92 129 197 887.3 0 197 130 692 887.3 0 692 131 615 887.3 0 615 132 525 887.3 0 525 133 151 887.3 0 151 134 176 887.3 0 176 135 173 887.3 0 173 136 122 887.3 0 122 137 160 887.3 0 160 138 140 887.3 0 140 139 92 887.3 0 92 140 134 887.3 0 134 141 129 887.3 0 129 142 350 887.3 0 350 143 891 887.3 0 891 144 2263 887.3 0 2263 145 216 887.3 0 216 146 62 887.3 0 62 147 88 887.3 0 88 148 67 887.3 0 67 149 95 887.3 0 95 150 107 887.3 0 107 151 147 887.3 0 147 152 418 887.3 0 418 153 119 887.3 0 119 154 126 887.3 0 126 155 110 887.3 0 110 156 232 887.3 0 232 157 142 887.3 0 142 158 139 887.3 0 139 159 117 887.3 0 117 160 126 887.3 0 126 161 114 887.3 0 114 162 212 887.3 0 212 163 222 887.3 0 222 164 300 887.3 0 300 165 338 887.3 0 338 166 423 887.3 0 423 167 1306 887.3 0 1306 168 2539 887.3 0 2539 169 53211 887.3 0 53211 170 20393 887.3 0 20393 171 22380 887.3 0 22380 172 227 887.3 0 227 173 112 887.3 0 112 174 173 887.3 0 173 175 78 887.3 0 78 176 378 887.3 0 378 177 62 887.3 0 62 178 116 887.3 0 116 179 93 887.3 0 93 180 95 887.3 0 95 181 94 887.3 0 94 182 81 887.3 0 81 183 58 887.3 0 58 184 93 887.3 0 93 185 102 887.3 0 102 186 92 887.3 0 92 187 59 887.3 0 59 188 59 887.3 0 59 189 90 887.3 0 90 190 69 887.3 0 69 191 83 887.3 0 83 192 147 887.3 0 147 193 256 887.3 0 256 194 170 887.3 0 170 195 66 887.3 0 66 196 72 887.3 0 72 197 66 887.3 0 66 198 86 887.3 0 86 199 62 887.3 0 62 200 95 887.3 0 95 201 81 887.3 0 81 202 109 887.3 0 109 203 114 887.3 0 114 204 110 887.3 0 110 205 116 887.3 0 116 206 104 887.3 0 104 207 71 887.3 0 71 208 80 887.3 0 80 209 64 887.3 0 64 210 80 887.3 0 80 211 76 887.3 0 76 212 70 887.3 0 70 213 71 887.3 0 71 214 77 887.3 0 77 215 91 887.3 0 91 216 92 887.3 0 92 217 88 887.3 0 88 218 81 887.3 0 81 219 68 887.3 0 68 220 57 887.3 0 57 221 243 887.3 0 243 222 249 887.3 0 249 223 150 887.3 0 150 224 888 887.3 0 888 225 97 887.3 0 97 226 88 887.3 0 88 227 81 887.3 0 81 228 100 887.3 0 100 229 85 887.3 0 85 230 134 887.3 0 134 231 188 887.3 0 188 232 113 887.3 0 113 233 87 887.3 0 87 234 81 887.3 0 81 235 114 887.3 0 114 236 166 887.3 0 166 237 341 887.3 0 341 238 257 887.3 0 257 239 94 887.3 0 94 240 100 887.3 0 100 241 85 887.3 0 85 242 106 887.3 0 106 243 80 887.3 0 80 244 60 887.3 0 60 245 81 887.3 0 81 246 101 887.3 0 101 247 88 887.3 0 88 248 66 887.3 0 66 249 79 887.3 0 79 250 71 887.3 0 71 251 74 887.3 0 74 252 74 887.3 0 74 253 163 887.3 0 163 254 119 887.3 0 119 255 117 887.3 0 117 256 80 887.3 0 80 257 73 887.3 0 73 258 75 887.3 0 75 259 75 887.3 0 75 260 74 887.3 0 74 261 98 887.3 0 98 262 157 887.3 0 157 263 197 887.3 0 197 264 122 887.3 0 122 265 68 887.3 0 68 266 95 887.3 0 95 267 86 887.3 0 86 268 82 887.3 0 82 269 107 887.3 0 107 270 132 887.3 0 132 271 559 887.3 0 559 272 1043 887.3 0 1043 273 822 887.3 0 822 274 277 887.3 0 277 275 73 887.3 0 73 276 90 887.3 0 90 277 101 887.3 0 101 278 82 887.3 0 82 279 230 887.3 0 230 280 150 887.3 0 150 281 104 887.3 0 104 282 91 887.3 0 91 283 94 887.3 0 94 284 87 887.3 0 87 285 110 887.3 0 110 286 91 887.3 0 91 287 75 887.3 0 75 288 92 887.3 0 92 289 99 887.3 0 99 290 115 887.3 0 115 291 99 887.3 0 99 292 191 887.3 0 191 293 165 887.3 0 165 294 135 887.3 0 135 295 98 887.3 0 98 296 251 887.3 0 251 297 223 887.3 0 223 298 175 887.3 0 175 299 85 887.3 0 85 300 80 887.3 0 80 301 79 887.3 0 79 Finished in 17.73 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 272,672 Reads with adapters: 64,118 (23.5%) Reads that were too short: 315 (0.1%) Reads written (passing filters): 63,803 (23.4%) Total basepairs processed: 60,928,221 bp Total written (filtered): 17,671,363 bp (29.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 64118 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 17 0.0 3 0 0 16 1 16 477 0.0 3 0 462 15 17 63046 0.0 3 61872 1143 28 3 18 172 0.0 3 11 154 6 1 19 28 0.0 3 1 0 22 5 20 40 0.0 3 1 0 0 39 21 1 0.0 3 0 0 0 1 22 1 0.0 3 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 1 0.0 3 1 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 3 0.0 3 1 0 0 2 53 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 84 1 0.0 3 1 86 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 216 3 0.0 3 0 0 0 3 217 280 0.0 3 0 0 8 272 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 4 0.0 3 0 0 0 4 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.78 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 63,803 Reads with adapters: 216 (0.3%) Reads written (passing filters): 63,803 (100.0%) Total basepairs processed: 17,671,363 bp Total written (filtered): 17,670,700 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 216 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.5% C: 51.4% G: 18.1% T: 6.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 205 996.9 0 205 4 8 249.2 0 8 5 2 62.3 0 2 6 1 15.6 0 1