This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 172.51 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 96,020 (2.6%) Reads that were too short: 10,422 (0.3%) Reads written (passing filters): 85,598 (2.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 23,534,304 bp (2.2%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 96020 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9418 887.3 0 9418 7 13146 887.3 0 13146 8 28860 887.3 0 28860 9 19683 887.3 0 19683 10 56 887.3 0 56 11 106 887.3 0 106 12 28 887.3 0 28 13 43 887.3 0 43 14 29 887.3 0 29 15 35 887.3 0 35 16 29 887.3 0 29 17 13 887.3 0 13 18 27 887.3 0 27 19 18 887.3 0 18 20 22 887.3 0 22 21 30 887.3 0 30 22 51 887.3 0 51 23 26 887.3 0 26 24 35 887.3 0 35 25 24 887.3 0 24 26 16 887.3 0 16 27 42 887.3 0 42 28 21 887.3 0 21 29 20 887.3 0 20 30 36 887.3 0 36 31 19 887.3 0 19 32 16 887.3 0 16 33 18 887.3 0 18 34 24 887.3 0 24 35 48 887.3 0 48 36 13 887.3 0 13 37 38 887.3 0 38 38 65 887.3 0 65 39 27 887.3 0 27 40 36 887.3 0 36 41 42 887.3 0 42 42 24 887.3 0 24 43 31 887.3 0 31 44 27 887.3 0 27 45 48 887.3 0 48 46 38 887.3 0 38 47 103 887.3 0 103 48 570 887.3 0 570 49 818 887.3 0 818 50 1005 887.3 0 1005 51 54 887.3 0 54 52 22 887.3 0 22 53 27 887.3 0 27 54 23 887.3 0 23 55 17 887.3 0 17 56 23 887.3 0 23 57 29 887.3 0 29 58 28 887.3 0 28 59 30 887.3 0 30 60 31 887.3 0 31 61 27 887.3 0 27 62 19 887.3 0 19 63 17 887.3 0 17 64 17 887.3 0 17 65 18 887.3 0 18 66 19 887.3 0 19 67 25 887.3 0 25 68 29 887.3 0 29 69 20 887.3 0 20 70 39 887.3 0 39 71 25 887.3 0 25 72 14 887.3 0 14 73 19 887.3 0 19 74 32 887.3 0 32 75 37 887.3 0 37 76 57 887.3 0 57 77 32 887.3 0 32 78 25 887.3 0 25 79 27 887.3 0 27 80 63 887.3 0 63 81 158 887.3 0 158 82 199 887.3 0 199 83 517 887.3 0 517 84 37 887.3 0 37 85 36 887.3 0 36 86 46 887.3 0 46 87 29 887.3 0 29 88 21 887.3 0 21 89 301 887.3 0 301 90 63 887.3 0 63 91 42 887.3 0 42 92 74 887.3 0 74 93 52 887.3 0 52 94 24 887.3 0 24 95 33 887.3 0 33 96 89 887.3 0 89 97 155 887.3 0 155 98 475 887.3 0 475 99 27 887.3 0 27 100 23 887.3 0 23 101 15 887.3 0 15 102 11 887.3 0 11 103 26 887.3 0 26 104 34 887.3 0 34 105 23 887.3 0 23 106 33 887.3 0 33 107 14 887.3 0 14 108 28 887.3 0 28 109 24 887.3 0 24 110 26 887.3 0 26 111 15 887.3 0 15 112 34 887.3 0 34 113 191 887.3 0 191 114 51 887.3 0 51 115 185 887.3 0 185 116 30 887.3 0 30 117 49 887.3 0 49 118 116 887.3 0 116 119 74 887.3 0 74 120 102 887.3 0 102 121 29 887.3 0 29 122 45 887.3 0 45 123 25 887.3 0 25 124 20 887.3 0 20 125 23 887.3 0 23 126 20 887.3 0 20 127 47 887.3 0 47 128 25 887.3 0 25 129 35 887.3 0 35 130 41 887.3 0 41 131 25 887.3 0 25 132 36 887.3 0 36 133 39 887.3 0 39 134 31 887.3 0 31 135 32 887.3 0 32 136 170 887.3 0 170 137 116 887.3 0 116 138 85 887.3 0 85 139 22 887.3 0 22 140 24 887.3 0 24 141 31 887.3 0 31 142 54 887.3 0 54 143 44 887.3 0 44 144 50 887.3 0 50 145 84 887.3 0 84 146 57 887.3 0 57 147 141 887.3 0 141 148 79 887.3 0 79 149 50 887.3 0 50 150 32 887.3 0 32 151 32 887.3 0 32 152 61 887.3 0 61 153 56 887.3 0 56 154 65 887.3 0 65 155 106 887.3 0 106 156 45 887.3 0 45 157 164 887.3 0 164 158 104 887.3 0 104 159 27 887.3 0 27 160 18 887.3 0 18 161 23 887.3 0 23 162 36 887.3 0 36 163 28 887.3 0 28 164 35 887.3 0 35 165 27 887.3 0 27 166 18 887.3 0 18 167 20 887.3 0 20 168 43 887.3 0 43 169 41 887.3 0 41 170 35 887.3 0 35 171 33 887.3 0 33 172 45 887.3 0 45 173 26 887.3 0 26 174 24 887.3 0 24 175 24 887.3 0 24 176 34 887.3 0 34 177 32 887.3 0 32 178 55 887.3 0 55 179 35 887.3 0 35 180 38 887.3 0 38 181 98 887.3 0 98 182 71 887.3 0 71 183 56 887.3 0 56 184 20 887.3 0 20 185 35 887.3 0 35 186 30 887.3 0 30 187 37 887.3 0 37 188 36 887.3 0 36 189 66 887.3 0 66 190 87 887.3 0 87 191 186 887.3 0 186 192 68 887.3 0 68 193 184 887.3 0 184 194 259 887.3 0 259 195 717 887.3 0 717 196 941 887.3 0 941 197 584 887.3 0 584 198 35 887.3 0 35 199 34 887.3 0 34 200 21 887.3 0 21 201 40 887.3 0 40 202 31 887.3 0 31 203 25 887.3 0 25 204 28 887.3 0 28 205 30 887.3 0 30 206 23 887.3 0 23 207 28 887.3 0 28 208 30 887.3 0 30 209 51 887.3 0 51 210 30 887.3 0 30 211 33 887.3 0 33 212 30 887.3 0 30 213 53 887.3 0 53 214 89 887.3 0 89 215 116 887.3 0 116 216 92 887.3 0 92 217 50 887.3 0 50 218 55 887.3 0 55 219 47 887.3 0 47 220 34 887.3 0 34 221 53 887.3 0 53 222 34 887.3 0 34 223 39 887.3 0 39 224 29 887.3 0 29 225 43 887.3 0 43 226 39 887.3 0 39 227 22 887.3 0 22 228 18 887.3 0 18 229 63 887.3 0 63 230 59 887.3 0 59 231 62 887.3 0 62 232 45 887.3 0 45 233 50 887.3 0 50 234 63 887.3 0 63 235 96 887.3 0 96 236 127 887.3 0 127 237 46 887.3 0 46 238 79 887.3 0 79 239 77 887.3 0 77 240 119 887.3 0 119 241 134 887.3 0 134 242 89 887.3 0 89 243 453 887.3 0 453 244 351 887.3 0 351 245 398 887.3 0 398 246 68 887.3 0 68 247 73 887.3 0 73 248 76 887.3 0 76 249 114 887.3 0 114 250 96 887.3 0 96 251 131 887.3 0 131 252 210 887.3 0 210 253 169 887.3 0 169 254 138 887.3 0 138 255 90 887.3 0 90 256 105 887.3 0 105 257 81 887.3 0 81 258 63 887.3 0 63 259 181 887.3 0 181 260 130 887.3 0 130 261 222 887.3 0 222 262 211 887.3 0 211 263 144 887.3 0 144 264 68 887.3 0 68 265 66 887.3 0 66 266 63 887.3 0 63 267 43 887.3 0 43 268 77 887.3 0 77 269 170 887.3 0 170 270 152 887.3 0 152 271 115 887.3 0 115 272 50 887.3 0 50 273 44 887.3 0 44 274 45 887.3 0 45 275 50 887.3 0 50 276 41 887.3 0 41 277 54 887.3 0 54 278 58 887.3 0 58 279 56 887.3 0 56 280 46 887.3 0 46 281 71 887.3 0 71 282 99 887.3 0 99 283 80 887.3 0 80 284 146 887.3 0 146 285 110 887.3 0 110 286 175 887.3 0 175 287 132 887.3 0 132 288 144 887.3 0 144 289 244 887.3 0 244 290 127 887.3 0 127 291 230 887.3 0 230 292 201 887.3 0 201 293 116 887.3 0 116 294 108 887.3 0 108 295 117 887.3 0 117 296 127 887.3 0 127 297 400 887.3 0 400 298 322 887.3 0 322 299 281 887.3 0 281 300 158 887.3 0 158 301 71 887.3 0 71 Finished in 6.89 s (80 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 85,598 Reads with adapters: 35,431 (41.4%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 35,403 (41.4%) Total basepairs processed: 23,534,304 bp Total written (filtered): 9,579,807 bp (40.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35431 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 16 0.0 3 0 0 14 0 2 19 365 0.0 3 0 330 17 10 8 20 21721 0.0 4 20749 806 133 29 4 21 235 0.0 4 22 189 14 5 5 22 12467 0.0 4 11906 442 83 29 7 23 62 0.0 4 9 17 2 26 8 24 4 0.0 4 0 0 1 2 1 25 28 0.0 4 0 0 0 23 5 41 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 79 1 0.0 4 1 80 1 0.0 4 0 0 0 0 1 86 21 0.0 4 0 0 0 0 21 96 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 316 0.0 4 0 0 0 0 316 128 4 0.0 4 0 0 0 0 4 129 150 0.0 4 0 0 0 0 150 130 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 222 2 0.0 4 0 0 0 1 1 228 7 0.0 4 0 0 0 1 6 229 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.14 s (60 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 35,403 Reads with adapters: 7,738 (21.9%) Reads written (passing filters): 35,403 (100.0%) Total basepairs processed: 9,579,807 bp Total written (filtered): 9,493,846 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7738 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 6.1% G: 90.6% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 553.2 0 11 4 11 138.3 0 11 6 6 8.6 0 6 7 2 2.2 0 2 9 2172 0.1 0 101 2071 10 4460 0.0 1 270 4190 11 992 0.0 1 17 975 12 17 0.0 1 0 17 13 7 0.0 1 0 7 138 1 0.0 1 1 150 31 0.0 1 26 5 151 4 0.0 1 3 1 152 2 0.0 1 2 153 1 0.0 1 0 1 208 3 0.0 1 2 1 212 1 0.0 1 1 223 14 0.0 1 14 224 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.